Expressão ectópica do gene NIK em tomateiros: Efeitos no desenvolvimento e na infecção por geminivírus

• Ano de defesa: 2007
• Autor(a) principal: Pires, Silvana Rodrigues
• Orientador(a): Fontes, Elizabeth Pacheco Batista
• Banca de defesa: Pereira, Maria Cristina Baracat , Fietto, Luciano Gomes
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Federal de Viçosa
• Programa de Pós-Graduação: Mestrado em Fisiologia Vegetal
• Departamento: Controle da maturação e senescência em órgãos perecíveis; Fisiologia molecular de plantas superiores
• País: BR
• Palavras-chave em Português: Tomate; NIK; Geminivirus; Transformação
• Palavras-chave em Inglês: Tomato; NIK; Geminivirus; Transformation
• Área do conhecimento CNPq: CNPQ::CIENCIAS BIOLOGICAS::BIOQUIMICA::BIOLOGIA MOLECULAR
• Link de acesso: http://locus.ufv.br/handle/123456789/4291

Geminivirus are plant virus packed as geminate semi- icosahedral particles and single-stranded circular DNA genomes. They can be either mono or bipartite and in the bipartite geminivirus, the genomic components are designated DNA-A and DNA-B. DNA-A encodes the proteins needed for replication, transactivation and encapsidation of the viral genome. DNA-B encodes two movement proteins, Movement Protein, MP, and Nuclear Shuttle Protein, NSP, both required for the establishment of a systemic infection. NSP transports the viral DNA between the nucleus and cytoplasm and acts cooperatively with MP to move the viral DNA cell-to-cell across the wall. The geminivirus are highly dependent on the host factors for proliferation and spread. NSP interacts with a plasma membrane receptor kinase, designated NIK (NSP- interacting kinase), from tomato, soybean and Arabidopsis. It has been demonstrated previously that inactivation of NIK gene enhances the susceptibility of the mutant to geminivirus infection. To elucidate the possible role of NIK in the protection mechanism to geminivirus infection, we investigated here the effects of NIK overexpression in the tomato response to geminivirus infection. The NIK gene from Arabidopsis thaliana was used to transform tomato. The primary transformants were confirmed by PCR, selected by RT-PCR according to the transgene expression, in vitro replicated and used for developmental analysis in vitro and in green house. NIK overexpression affected the overall developmental performance of transgenic lines which display delayed and stunted growth, with lower stem, less developed root and shoot systems and a leaf curly phenotype. These transgenic line phenotypes overexpression NIK were similar to that displayed by inhibition of Brassinosteroid synthesis or signaling. Infectivity assays in tomato with ToYSV-[MG-Bi2] revealed that overexpression of NIK caused attenuation of symptom, as the transgenic lines developed less severe symptoms in comparison with the untransformed lines. These results are consistent with a protective role of NIK against viral infection and suggest that the NIK-mediated signaling communicates to some level with the brassinosteroid signaling.