Impacto de Tetranychus evansi, Tetranychus urticae e Tuta absoluta sobre a via das lipoxigenases do tomateiro e as proteases digestivas destes herbívoros

• Ano de defesa: 2014
• Autor(a) principal: Vargas, Manuel Antônio Solís
• Orientador(a): Oliveira, Maria Goreti de Almeida
• Banca de defesa: Pereira, Eliseu José Guedes , Silva, Camila Rocha da , Ferreira, João Alfredo Marinho
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Federal de Viçosa
• Programa de Pós-Graduação: Mestrado em Entomologia
• Departamento: Ciência entomológica; Tecnologia entomológica
• País: BR
• Palavras-chave em Português: Relação inseto-planta; Tetranychus; Tuta absoluta; Lioxigenases; Inibidores de proteases
• Palavras-chave em Inglês: Insect-plant relationship; Tetranychus; Tuta absoluta; Lioxigenases; Protease inhibitors
• Área do conhecimento CNPq: CNPQ::CIENCIAS BIOLOGICAS::BIOQUIMICA::ENZIMOLOGIA
• Link de acesso: http://locus.ufv.br/handle/123456789/3990

The spider mite Tetranychus evansi interferes in the lipoxygenases pathway of plant defenses respond where are produced jasmonic acid that activates expressing genes of proteinase inhibitors which affect the capability of digest proteins in herbivores. The not PI induction ability of T. evansi promotes his performance and reproduction but other species too like the spider mite T. urticae. Until now, there has not been study how digestive enzyme activity of these and other herbivores could be affected by T. evansi interference in tomato defense respond neither which digestive enzymes are present in this particular plant- herbivore relation what became in our research objective. Tomato plants were infested with T. evansi, T. urticae or moth larvae Tuta absoluta, while some plants were infested with a combination of T.evansi with each one of the other species simultaneously. When T. evansi was in the same plant with T. urticae the defense respond by lipoxygenases pathway were induced and the proteinase inhibitors were equal that plants attacked by only T.urticae, and plants attacked by T. evansi and T. absoluta simultaneously. T. evansi oviposition rate were only affected in plants previously attacked by T. urticae. In plants previously attacked by the combination of herbivores or just T. absoluta the oviposition rate doesn t differs of rates in clean plants, despite of high PI concentration. This suggests that T. evansi performance is affected by PI but for T. urticae plant injuries and traces and T. evansi has to overcome more factors involved in food competition. Digestive enzymes profile suggest a higher serine than cysteine proteinase activity been mainly tripsyn-like proteinases. In spider mites, tripsyn-like proteinases increase when PI were higher, possible to overcome PI toxic effects plus a higher aminoacids demand in T. evansi when competes with T. urticae for food resources. The quimotripsyn-like proteinases seems to be sensible to PI and decrease with high tripsyn-like ativity.