Diversidade de bactérias endofíticas em frutos de Coffea canephora em três estádios de maturação

• Ano de defesa: 2011
• Autor(a) principal: Miguel, Paulo Sérgio Balbino
• Orientador(a): Borges, Arnaldo Chaer
• Banca de defesa: Silva, Daniele Ferreira da , Zerbini, Poliane Alfenas
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso embargado
• Idioma: por
• Instituição de defesa: Universidade Federal de Viçosa
• Programa de Pós-Graduação: Mestrado em Microbiologia Agrícola
• Departamento: Associações micorrízicas; Bactérias láticas e probióticos; Biologia molecular de fungos de interesse
• País: BR
• Palavras-chave em Português: Microbiota endofítica; Filogenia molecular; Frutos de café
• Palavras-chave em Inglês: Endophytic microbiota; Molecular phylogeny; Coffee fruits
• Área do conhecimento CNPq: CNPQ::CIENCIAS AGRARIAS::AGRONOMIA::FITOSSANIDADE::MICROBIOLOGIA AGRICOLA
• Link de acesso: http://locus.ufv.br/handle/123456789/5340

Endophytic bactéria in fruits inhabits internal tissues without cause visible damage or symptons. However, there is no report of their presence and the diversity in Coffea canephora fruits. Thereby, this work aimed to determine the existence and diversity of cultivable endophytic bacteria in fruits of that specie in three maturation stages. Bacterial isolation and quantification were performed in R2A medium and resulted in 140 isolates gathered in 21 morphotypes, being 55 % of Gram-positive bacteria. The community is composed by 14 genera and 18 bacterial species belonging to phylum Actinobacteria, Firmicutes, Bacteroides, Alpha and Gamma- Proteobacteria. For the first time, Kocuria turfanensis and Pantoa vagans are identified as endophytic species. From 18 species identified as endophytic in C. canephora fruits, seven are not described as endophytic in fruits, namely: Bacillus thuringiensis, Bacillus licheniformis, Agrobacterium tumefaciens, Escherichia coli, Enterobacter hormaechei, Chryseobacterium sp. and Ochrobactrum sp.. The diversity of cultivable endophytic bacteria was distinct in the three maturation stages of C. canephora fruits, being larger in green fruits, where Bacillus subtilis was predominant. The number of Gram-positive isolates was higher than that for Gram-negative in the first two development stage, while in mature fruits the number of colonies from both Gram-positive and Gram-negative was similar. In the last maturation stage, diversity of endophytic bacteria species was smaller and Klebsiella oxytoca was the predominant specie, what was assigned to probable effects of higher caffeine and sugar concentration in the fruits.