Micropropagação e enraizamento de miniestacas de clones híbridos de Eucalyptus globulus

• Ano de defesa: 2009
• Autor(a) principal: Borges, Silvano Rodrigues
• Orientador(a): Xavier, Aloisio
• Banca de defesa: Dias, José Maria Moreira , Takahashi, Elizabete Keiko
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Federal de Viçosa
• Programa de Pós-Graduação: Mestrado em Ciência Florestal
• Departamento: Manejo Florestal; Meio Ambiente e Conservação da Natureza; Silvicultura; Tecnologia e Utilização de
• País: BR
• Palavras-chave em Português: Miniestaquia; Propagação in vitro; Clonagem
• Palavras-chave em Inglês: Mini-cuttings; In vitro propagation; Clones
• Área do conhecimento CNPq: CNPQ::CIENCIAS AGRARIAS::RECURSOS FLORESTAIS E ENGENHARIA FLORESTAL::SILVICULTURA
• Link de acesso: http://locus.ufv.br/handle/123456789/2998

The present study aimed to evaluate Eucalyptus urophylla x E. globulus and Eucalyptus grandis x E. globulus clones in relation to micropropagation by axillary shoot proliferation in the in vitro establishment and multiplication phases, and the rooting of mini-cuttings in the clonal propagation of these hybrids. In the in vitro establishment phase, contamination by fungi and bacteria, browning and nodal segment shoot inductions of 21 Eucalyptus urophylla x E. globulus clones and eight Eucalyptus grandis x E. globulus clones were evaluated in different in vitro introductions. In the in vitro multiplication phase, the multiplication rate of 21 Eucalyptus urophylla x E. globulus clones and six Eucalyptus grandis x E. globulus clones was evaluated in successive subcultures using MS and JADS culture media in different in vitro introductions. In the mini- cuttings, the survival and emission of roots at the bottom of the dibble tube in the green house and shade house was evaluated, and the rooting and number of roots of apical and intermediary mini-cuttings in the shade house, treated with IBA, in nine Eucalyptus urophylla x E. globulus clones and two Eucalyptus grandis x E. globulus clones. In the in vitro establishment phase, shoot induction varied among clones, being satisfactory for most of the genotypes, while the browning rate and bacterial and fungal contamination did not compromise the establishment of the cultures. In the in vitro multiplication phase, a differentiated response for the multiplication rate was noted, with clones presenting good in vitro multiplication rates and recalcitrant others for micropropagation. The MS and JADS culture media had positive effects, with greater multiplication rate tendencies for the MS media, where the multiplication rate of the subcultures varied with the tendency of an initial increase and subsequent fall in most of the clones and in vitro introductions. As for the mini-cuttings, there was no significant effect of IBA in any of the studied characteristics. The survival rate of the mini-cuttings was high for most of the clones and the rooting rate varied among clones and the types of mini-cuttings with superior results for apical mini- cuttings. A high variability of responses to rooting was observed among the studied clones, with clones presenting a high rate of rooting and number of roots for minicuttings, while some clones still lacked adjustments to the mini- cuttings propagation process.