Aloenxerto meniscal de coelho: avaliações da morfologia, da vascularização e da técnica cirúrgica

• Ano de defesa: 2013
• Autor(a) principal: Vilela, Liana Mesquita
• Orientador(a): Carlo, Ricardo Junqueira Del
• Banca de defesa: Pontes, Kelly Cristine de Sousa , Duarte, Tatiana Schmitz , Monteiro, Betânia Souza , Pompermayer, Luiz Gonzaga
• Tipo de documento: Tese
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Federal de Viçosa
• Programa de Pós-Graduação: Doutorado em Medicina Veterinária
• Departamento: Biotecnologia, diagnóstico e controle de doenças; Epidemiologia e controle de qualidade de prod. de
• País: BR
• Palavras-chave em Português: Meniscectomia; Meniscos; Aloenxerto meniscal
• Palavras-chave em Inglês: Meniscectomy; Menisci; Meniscal allograft
• Área do conhecimento CNPq: CNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIA::CLINICA E CIRURGIA ANIMAL::CLINICA CIRURGICA ANIMAL
• Link de acesso: http://locus.ufv.br/handle/123456789/1459

The treatment choice for lesions on meniscus is suture, which cannot be done in all cases, allowing alternatively the meniscus injury removal, which causes degenerative joint. One option to avoid these changes is replacement the meniscus by allografts. Thus, this study aimed to assess the feasibility the technique medial meniscal allograft in rabbits, perform macro and microscopic analysis the allografts and evaluate vascularization the grafted material, comparing them with the menisci contralateral medial, not deployed. Were used 16 rabbits adult male New Zeland, who underwent left medial meniscectomy and meniscus implantation, previously preserved in 98% glycerin for at least 45 days. After 70 days the transplant was performed euthanasia in animals, and the transplanted meniscuses (left knee), called meniscuses treated (MT1 to MT16) and contralateral meniscuses (right knee), called meniscuses own (MP1 to MP16) were removed. Sixteen meniscuses, 8 treated and 8 own, were submitted to macroscopic and histomorphometric, by quantifying the types collagen fibers and evaluating their disposal, and quantification a cell types. The other sixteen meniscuses, 8 treated and 8 own, underwent vascularization study, through radiographs contrasted. The length the meniscuses own (MP) ranged from 0.9 to 1.0 cm, while the meniscuses treated (MT), two showed smaller than the own. Only a meniscus transplanted showed partial fixation, being fixed only by cranial horn, this may have occurred due to rupture or early absorption the suture, the remaining meniscuses had been completely fixed the synovial membrane. In meniscuses own (MP) and treated (MT), were found similar percentages of chondrocytes, fibroblasts and fibrocytes, with predominance a chondrocytes in the meniscal horns, while other cell types, were mainly present in the meniscal body. Were observed in treated menisci (MT), bundles of collagen fibers arranged in an organized manner in body, and disorganized, especially in the horns. This disorganization is result the process tissue remodeling and is temporary, as demonstrated in other studies. Already in MP, the fibers were organized all extension the meniscus. The concentrations of collagen type I and III present in MT were similar to those observed in MP. Was visualized vascularization in perimeniscais tissues, and peripheral edge of the meniscuses treated, being similar to own. Therefore, the allografts were invaded by vessels the host, favoring fixation.