Mapeamento de QTL para conteúdo de ácido oléico em soja utilizando marcadores SNP

• Ano de defesa: 2012
• Autor(a) principal: Matta, Lorêta Buuda da
• Orientador(a): Moreira, Maurílio Alves
• Banca de defesa: Silva, Luciano da Costa e , Arruda, Klever Márcio Antunes
• Tipo de documento: Tese
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Federal de Viçosa
• Programa de Pós-Graduação: Doutorado em Genética e Melhoramento
• Departamento: Genética animal; Genética molecular e de microrganismos; Genética quantitativa; Genética vegetal; Me
• País: BR
• Palavras-chave em Português: Ácidos graxos; Glycine max; MIM
• Palavras-chave em Inglês: Fatty acid; Glycine max; MMI
• Área do conhecimento CNPq: CNPQ::CIENCIAS AGRARIAS::AGRONOMIA::FITOTECNIA::MELHORAMENTO VEGETAL
• Link de acesso: http://locus.ufv.br/handle/123456789/1351

The primary breeding goal for soybean oil quality is to enhance the oxidative stability by increasing the level of oleic acid and concomitant reduction of linolenic and linoleic acid content. Identification of quantitative trait loci (QTL) associated with oleic acid content can advance the application of marker-assisted selection, providing the combination of favorable alleles from different genetics resources. Thus, the objective of this study was map QTL for oleic acid content in soybean using SNP markers. The following steps were performed: (1) estimation of genetic parameters and correlations between fatty acid and oil content, (2) construction of genetic linkage map with SNP markers in F2 population derived from FA22 x CS303TNKCA, (3) identifying QTL associated with oleic acid content and other unsaturated fatty acids. The oleate heritability estimates indicate that effective selection can be practiced in early generations. Results of the correlations indicate that increases in the levels of oleic acid result in reduction of other unsaturated fatty acids and soybean oil. However, the greater magnitude of these correlations were found between oleic and linoleic acids. This study revealed a novel oleate QTL on linkage group D1B, with moderate effect near the SNP marker BARC-007889-00156, which was detected in almost all environments evaluated. QTL with small and moderate effects were reported on linkage groups D1A, C1, C2, K and H. However, only the QTL on linkage group C1 was detected in two environments and the combined analyses. In most cases, linoleate QTL correspond to oleate QTL and their effects had opposite directions. A major linolenate QTL was detected on linkage group B2 explaining most of the phenotypic variation.