Tratamento de defeitos críticos em calvária de camundongos com células-tronco mesenquimais associadas ou não ao plasma rico em plaquetas

• Ano de defesa: 2009
• Autor(a) principal: Monteiro, Betânia Souza
• Orientador(a): Carlo, Ricardo Junqueira Del
• Banca de defesa: Nardi, Nance Beyer , Paula, Sérgio Oliveira de
• Tipo de documento: Tese
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Federal de Viçosa
• Programa de Pós-Graduação: Doutorado em Medicina Veterinária
• Departamento: Biotecnologia, diagnóstico e controle de doenças; Epidemiologia e controle de qualidade de prod. de
• País: BR
• Palavras-chave em Português: Células-tronco mesenquimais; Reparação óssea; Camundongos
• Palavras-chave em Inglês: Mesenchymal stem cells; Bone repair; Mice
• Área do conhecimento CNPq: CNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIA::CLINICA E CIRURGIA ANIMAL
• Link de acesso: http://locus.ufv.br/handle/123456789/1431

Bone tissue is continuously subjected to dynamic remodeling processes that are important for tissue homeostasis maintenance and micro injuries repair. This repair is a complex process that involves chemotaxis and adhesion of undifferentiated and young cells to demineralized bone matrix and sequential proliferation and differentiation of osteoblasts, followed by a phase of organization of the formed tissue. Literature evidences the participation of regulatory proteins coordinating the cell and molecular biology of osteogenesis and among these proteins are the growth factor produced and released by platelet-rich plasma (PRP) and the mesenchymal stem cells (MSC). Prospects of establishing cell therapies using the PRP and the MSC to treat injured tissues and/or with little capacity for regeneration are promising and clinical studies are being conducted, showing very encouraging results. This study evaluated the contribution of MSC used isolated and associated with platelet-rich plasma in the bone repair processes of critical defects produced in calvarial of isogenic mice C57BL/6. Four experimental groups were evaluated, each group containing 12 mice that received different treatments with MSC pellet, platelets in gel, association of MSC/PRP and a group didn t received treatment. Macroscopic, statistics, microscopic and molecular evaluations were realized and demonstrated that there was positive contribution for repairing bone critical defects produced in calvarial of young mice treated with mesenchymal stem cells (MSC) derived from bone marrow of mice C57BL / 6 GFP +, isolated and associated with of PRP, and platelet in gel isolated. Among all the realized treatments the better results were obtained in the groups that used MSC alone or combined with PRP, followed by the group treated with PRP alone, and finally, the control group.