Retrotransposon LTR no genoma de Moniliophthora perniciosa e Cochliobolus heterostrophus

• Ano de defesa: 2009
• Autor(a) principal: Santana, Mateus Ferreira
• Orientador(a): Queiroz, Marisa Vieira de
• Banca de defesa: Moraes, Célia Alencar de , Costa, Maurício Dutra
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Federal de Viçosa
• Programa de Pós-Graduação: Mestrado em Microbiologia Agrícola
• Departamento: Associações micorrízicas; Bactérias láticas e probióticos; Biologia molecular de fungos de interesse
• País: BR
• Palavras-chave em Português: Retrotransposon; Moniliophthora perniciosa; Cochliobolus heterostrophus
• Palavras-chave em Inglês: Retrotransposon; Moniliophthora perniciosa; Cochliobolus heterostrophus
• Área do conhecimento CNPq: CNPQ::CIENCIAS BIOLOGICAS::GENETICA::GENETICA MOLECULAR E DE MICROORGANISMOS
• Link de acesso: http://locus.ufv.br/handle/123456789/5323

Gypsy/Ty3 group retrotransposons are the main transposable elements found in the genome of pathogenic fungi. In this work, two retrotransposons termed MpSaci and Sophie were analyzed in Moniliophthora perniciosa and Cochliobolus heterostrophus, respectively. MpSaci was used to assess molecular markers based on transposable elements IRAP and REMAP. When 70 isolates of M. perniciosa were analyzed, a total of 43 loci were amplified by generating 46.51% polymorphism. Significant differences were found between populations of M. perniciosa divided in relation to biotope and the geographical origin, showing that populations are structured on the geographical origin and the host (biotype). The cluster analysis of different geographical regions of biotype C were observed two large groups that show two main entrances of the pathogen in the state of Bahia. Searches done in the database of the Genome Project of C. heterostrophus (JGI- Genome) showed the presence of DNA sequences with similarity to reverse transcriptase of Class I elements belonging to the group Gypsy/Ty3. Based on these sequences could be found seven different copies of an intact transposable element named Sophie. The analysis of 37 sequences of the reverse transcriptase gene showed a possible silencing mechanism similar to RIP. The seven copies of the element Sophie had 7426 bp 7512 bp. The retrotransposon sequences Sophie has two open reading frames (ORFs) that encode the Gag protein and the Pol polyprotein. The presence of different target sites suggests that Sophie is an element with recent activity in the genome of C. heterostrophus can have a great impact on the evolution of the genome of its host.