O corpo gorduroso de Lutzomyia longipalpis e Phlebotomus papatasi (Diptera: Psychodidae: Phlebotominae): estudo morfológico em diferentes condições

• Ano de defesa: 2013
• Autor(a) principal: Assis, Wiviane Alves de
• Orientador(a): Martins, Gustavo Ferreira
• Banca de defesa: Lisboa, Luciane Cristina Oliveira , Azevedo, Dihego de Oliveira
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Federal de Viçosa
• Programa de Pós-Graduação: Mestrado em Biologia Celular e Estrutural
• Departamento: Análises quantitativas e moleculares do Genoma; Biologia das células e dos tecidos
• País: BR
• Palavras-chave em Português: Lutzomyia longipalpis; Phlebotomus papatasi; Corpo gorduroso
• Palavras-chave em Inglês: Lutzomyia longipalpis; Phlebotomus papatasi; Fat body
• Área do conhecimento CNPq: CNPQ::CIENCIAS BIOLOGICAS::BIOLOGIA GERAL
• Link de acesso: http://locus.ufv.br/handle/123456789/2369

The Phlebolomus and Lutzomyía sandflies are found in the Old and New World, respectively. Their adult females are haematophagous and important vectors of viruses, bacteria and various species of Leíshmanía. Despite their importance, some aspects of their physiology are poorly studied yet, including the organization of your fat body (FB). The FB is a multifunctional organ and its functions include storage of nutrients (such as proteins, carbohydrates and lipids). The aim of this study was to investigate the FB of adult females of Lutzomyía longípaüaís and Phlebolomus papalasí, including the subcellular changes that occur in the FB trophocytes after blood feeding, and also study the effect of polyclonal antisera against the midgut peritrophic matrix proteins (i.e. chitinase and peritrophin) in the lipid accumulation in the trophocytes. Two groups of females were used of each species: 45h after blood feeding and the control, fed with sucrose solution (20-30%). Samples were processed for light and electron microscopy, and for morphometric analysis, trophocyte lipid droplets were considered. For the females that receive the blood diet with antibodies, the lipid droplet areas were measured 24, 48 and 72h after feeding. The FB is quite similar between the two species in terms of location, organization and cellular composition. The FBs are distributed throughout the whole insect body and are organized as parietal and perivisceral lobes. The FBs include the trophocytes and oenocytes. The trophocytes are the most abundant cells, rich in mitochondria and RER mainly in blood fed females. The oenocytes are rich in smooth endoplasmic reticulum, suggesting their role in lipid processing. Although L. longípahyís and P. papatasí are autogenous and anautogenous, respectively, they share subcellular changes observed after blood feeding, even in the former species the blood acquisition is not mandatory for the egg development. In addition, in non-blood fed and in blood fed individuals, there is no significant differences in the lipid droplet area in the trophocytes of L. longípahyís and P. papatasí. The treatment with antibodies has been shown to influence the supply of nutrients by FB. In L. longípaüaís 24h after feeding showed no significant difference. Conversely, 48h after blood feeding significant differences in the lipid droplet control is almost two times higher than the antiperitrophin and antichitinase fed females However, among the females treated with these two antibodies there is no difference in terms of lipid droplet area. Seventy two h after feeding, the droplets area in antisera fed females was almost two times higher than the control. In P. papatasí, the lipid droplets area 24h after the treatment with antiserum against chitinase is almost three-fold greater compared to the control, while 48h and 72h after feeding the area was not significantly different among females treated with antisera against the chitinase and peritrophin. Our results suggest that the antibodies were able to negatively interfere with the digestion process in L. longípalpís and P. papatasí from 48h followed the blood meal, even if they were developed from P. papatasz'. This work constitutes and provides important information for the better understanding of the sandflies FB biology.