Prevalência de adenovírus como agente causador de conjuntivite em clínica médica oftalmológica especializada nos anos de 2009 e 2010

• Ano de defesa: 2011
• Autor(a) principal: Rosado Filho, Euldes Nei
• Orientador(a): Oliveira, Leandro Licursi de
• Banca de defesa: Silva, Eduardo de Almeida Marques da , Silva, Cynthia Canedo da
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Federal de Viçosa
• Programa de Pós-Graduação: Mestrado em Biologia Celular e Estrutural
• Departamento: Análises quantitativas e moleculares do Genoma; Biologia das células e dos tecidos
• País: BR
• Palavras-chave em Português: Adenovírus; Conjuntivite; PCR
• Palavras-chave em Inglês: Adenovirus; Conjunctivitis; PCR
• Área do conhecimento CNPq: CNPQ::CIENCIAS BIOLOGICAS::BIOLOGIA GERAL
• Link de acesso: http://locus.ufv.br/handle/123456789/2339

The adenovirus (AdV) is one of the most common etiological agents of conjunctivitis, being associated with outbreaks and epidemics of fast widespread conjunctivitis and might also cause upper respiratory tract infections, cystitis, gastroenteritis and scenarios of more serious infections such as pneumonia and myocarditis. The viral conjunctivitis shows up as redness in the eyes, tearing, watery eye, photophobia, ocular discomfort and often also as visual impairment due to keratitis/keratopathy. In this study, it had been sought to assess the prevalence of the adenovirus as the etiological agent of conjunctivitis in an ophthalmological clinic in the city of Viçosa, Minas Gerais, Brazil, from January 2009 to December 2010. Serotypes present in 11 positive randomly selected samples have been characterized by sequencing and genotyping. In order to define the frequency of adenovirus as the etiological agent of conjunctivitis, samples were collected from the conjunctival secretion of 91patients. After DNA extraction, samples were subjected to PCR with primers specific for Adenovirus. Samples that had the adenovirus, there has been an amplification of a band of 301 bp, corresponding to a part of the structural gene II protein, hexoncapsid, common to multiple adenoviruses. Based on the findings of evolution and ophthalmological examination with slit-lamp serial biomicroscopy, there was clinical suspicion of Adenovirus prevalence of 43.68%. The results of the PCR test showed a prevalence of Adenovirus in 36.3% of the samples. Eleven PCR + samples were sequenced, revealing the presence of serotypes 8, 4, 7, 3, 34 and one unclassified in group D. The presence of serotypes 8, 4, 3 and 34 was not surprising, however, of serotype 7 (group B) was unexpected, because there are reports associated with cases of unfavourable and fatal infection evolution, caused by variants of this type, due pneumonia and myocarditis. The presence of adenovirus in conjunctival secretion indentified through PCR allows a precise diagnosis, and avoiding unnecessary and expensive antibiotics, permitting restrictive measures of contact and dissemination and warning of the presence of more aggressive serotypes causing epidemic keratoconjunctivitis, among other etiological agents of pneumonia, myocarditis and gastroenteritis.