Efeitos do extrato aquoso da raiz de Ouratea semiserrata (Mart.) Engl. (Ochnaceae) sobre os testículos de camundongos suíços adultos

• Ano de defesa: 2008
• Autor(a) principal: Matta, Ana Paula de Lima Florentino
• Orientador(a): Matta, Sérgio Luis Pinto da
• Banca de defesa: Neves, Clóvis Andrade , Melo, Fabiana Cristina Silveira Alves de , Fonseca, Cláudio César
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Federal de Viçosa
• Programa de Pós-Graduação: Mestrado em Biologia Celular e Estrutural
• Departamento: Análises quantitativas e moleculares do Genoma; Biologia das células e dos tecidos
• País: BR
• Palavras-chave em Português: Reprodução; Morfometria testicular; Raiz de bugre; Célula de Leydig; Fitoterapia
• Palavras-chave em Inglês: Reproduction; Testicular morphometry; Ouratea semiserrata; Leydig cell; Phytotherapy
• Área do conhecimento CNPq: CNPQ::CIENCIAS BIOLOGICAS::BIOLOGIA GERAL
• Link de acesso: http://locus.ufv.br/handle/123456789/2307

The utilization of plants with aphrodisiac action is a practice that has been increasing in the last years, a fact that awakes the interest of researchers from phytochemical, pharmacological and clinic investigations. Ouratea semiserrata is popularly used as astringent, anti- inflammatory and sexual stimulator. In this study, the goal was to evaluate the effects of aqueous extract from roots of O. semiserrata (AEROS) over the body and testicular biometry, seminiferous tubule and intertubule components of adult mice testes, also the extract being subjected to phytochemical prospection and the quantification of phenolics compounds. Twenty seven animals were used, divided into a control group (G1), which received distilled water, and two groups that received AEROS in the concentrations of 750 (G2) and 1500 (G3) mg/kg of body weight. The phytochemical prospection by thin layer chromatography revealed the presence of triterpenes/steroids, flavonoids, essential oils and tannins. The content of total polyphenols, proanthocyanidins, and flavonoids in the plant root were 3.57%, 2.07% e 0.11%, respectively. It is suggested that AEROS aphrodisiac potential is probably related to the presence of flavonoids and proanthocyanidins, compounds with vasodilatation activity. The treatment in both extract concentrations interfered in no significant way into body and testicular biometry and tubular morphometry, suggesting absence of alterations in the spermatogenic process and root toxicity. A reduction in volume proportion and connective tissue volume in both groups treated was present. The highest extract concentration promoted an increase in Leydig nuclear diameter and volume, and a decrease in macrophage proportion and blood vessels volume. Alterations in Leydig nuclear diameter and volume may be associated to increased levels of testosterone, probably, not enough to act over this testosterone-dependent reproductive organs weight.