Transformações do corpo gorduroso durante a metamorfose de Melipona quadrifasciata (HYMENOPTERA: APIDAE)

• Ano de defesa: 2013
• Autor(a) principal: Santos, Douglas Elias
• Orientador(a): Serrão, José Eduardo
• Banca de defesa: Martins, Gustavo Ferreira , Santana, Weyder Cristiano
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Federal de Viçosa
• Programa de Pós-Graduação: Mestrado em Biologia Celular e Estrutural
• Departamento: Análises quantitativas e moleculares do Genoma; Biologia das células e dos tecidos
• País: BR
• Palavras-chave em Português: Morte celular programada; Melipona quadrifasciata; Corpo gorduroso; Metamorfose
• Palavras-chave em Inglês: Programmed cell death; Melipona quadrifasciata; Greasy body; Metamorphosis
• Área do conhecimento CNPq: CNPQ::CIENCIAS BIOLOGICAS::BIOLOGIA GERAL
• Link de acesso: http://locus.ufv.br/handle/123456789/2366

The development of the bee Melipona quadrifasciata comprises five days of embryonic development, 15 days of larval stage and 18 days of pupal stage. In this period several tissues and organs undergo changes. The fat body is an organ composed basically trophocytes, which suply energy during insect metamorphosis. During metamorphosis the fate of fat body can be diffetent: they can be renewal after destruction of the larval and they can are recycled and relocated to form the fat body the adult insect. The aim of this study was to identify the occurence j of programmed cell death in the fat body cells during metamorphosis of M. quadrifasciata. Post-defecating larvae, white-, pink-, brown- and black-eye pupae had the in fat body dissected and subjected to immunodetection for cleaved caspase-3, DNA integrity, ultrastructural analysis, detection of autophagy by means of RT-PCR for the gene Atg1. Few apoptotic cells were seen in the fat body of M. quadrifasciata by there are fewer cleaved caspase-3 positive cells and absence of fragmented DNA. So corroborating, under electron microscope these cells showed an increasing in the number of mitochondria and free ribosomes in the cytoplasm as well as the presence of nucleus with descondensed chromatin throughout metamorphosis. The fat body cells of post-defecating larvae of M. quadrifasciata express higher amounts of Atg1 mRNA than pupae. These results suggest that there are few fat body cells of M. quadrifasciata that undergo cell death by apoptosis during the process of metamorphosis, but with evidence of increased autophagy during post-defecating larvae stage.