Estratégia de superação da autoincompatibilidade e herança da sensibilidade da indução floral ao fotoperíodo em maracujazeiro

• Ano de defesa: 2013
• Autor(a) principal: Lira Júnior, José Severino de
• Orientador(a): Bruckner, Claudio Horst
• Banca de defesa: Pereira, Messias Gonzaga , Moura, Waldênia de Melo
• Tipo de documento: Tese
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Federal de Viçosa
• Programa de Pós-Graduação: Doutorado em Genética e Melhoramento
• Departamento: Genética animal; Genética molecular e de microrganismos; Genética quantitativa; Genética vegetal; Me
• País: BR
• Palavras-chave em Português: Passiflora edulis; Autofecundação; Mutante; Epistasia
• Palavras-chave em Inglês: Passiflora edulis; Selfing; Mutant; Epistasis
• Área do conhecimento CNPq: CNPQ::CIENCIAS AGRARIAS::AGRONOMIA::FITOTECNIA::MELHORAMENTO VEGETAL
• Link de acesso: http://locus.ufv.br/handle/123456789/1360

Selfing procedures in passion fruit (Passiflora edulis) are inefficient. The reaction of self-incompatibility inhibits the pollen tube growth from the same plant or flowers of incompatible plants. The passion fruit has limitations for obtaining inbred lines, essential to the production of hybrid and for studies of inheritance among parents homozygous and contrasting for interest characteristics. Mutant plants of Passiflora edulis, able to bloom under reduced photoperiod, were identified in the breeding program of passion fruit of the Federal University of Viçosa (UFV), State of Minas Gerais, Brazil. Understanding the genetic control of photoperiod insensibility facilitates the incorporation of this phenotype in hybrids or cultivars, recommended to cultivation in higher latitudes. Furthermore, these mutants allow studies on understanding the genetic control of floral induction insensibility in passion fruit. We propose a new methodology of selfing for the passion fruit. Self-pollination was proposed at anthesis with the stigma excised, compared with self-pollination at floral buds and anthesis stage. There were also evaluated three times of self-pollination (13 h, 17 h and 13:17 h). These procedures and schedules were evaluated in two genotypes (M7 and N9). There was significance (p<0.05) of the procedures and schedules interaction. The self-pollinations without stigmas at anthesis, at 13h and repeated the same day at 17h, reached fruit set percentages of 83.33% (M7) and 80.56% (N9). There was no fruit set at self-pollinations at anthesis, to 13h or 17h, but also at anthesis without stigma to 17 h. The proposed new methodology, with one self-pollination (13h) at anthesis without stigma, is efficient in the production of passion fruit seeds. In inheritance study, the objectives were to determine the minimum number of loci and the type of interaction intra-loco and inter-locos, involved in controlling early blooming of a mutant genotype of passion fruit, insensitive to changes in photoperiod, under latitude 20 ° 45'14'' S. In selfing progeny of the mutant insensitive parent (M7), the hypothesis of three loci was not rejected by the chi-square statistical test. His expected phenotypic ratio, 27:37 (insensitive: normal), and the number of plants observed, 13:19 (insensitive: normal), reached the highest associated probability of 85.79%. In sum value of the hybridization among insensitive parent (M7) and normal parent (N9), and its reciprocal, the three loci hypothesis was not rejected by the chi-square statistical test. His expected phenotypic ratio, 1:7 (insensitive:normal), and the number of plants observed, 7:56 (insensitive:normal), obtained the associated probability of 73.88%. The insensibility to photoperiod (PI), exhibited by parent M7, is probably controlled by at a minimum three loci in heterozygous (PI1, PI2 and PI3), with complete dominance relationship between alleles.