Proliferação, morte celular e ação enzimática no intestino médio de abelhas (Hymenoptera, Apidae) durante a metamorfose

• Ano de defesa: 2012
• Autor(a) principal: Cruz, Lilian Cota
• Orientador(a): Neves, Clóvis Andrade
• Banca de defesa: Fialho, Maria do Carmo Queiroz , Santos, Conceição Aparecida dos , Oliveira, Leandro Licursi de , Lino Neto, José
• Tipo de documento: Tese
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Federal de Viçosa
• Programa de Pós-Graduação: Doutorado em Biologia Celular e Estrutural
• Departamento: Análises quantitativas e moleculares do Genoma; Biologia das células e dos tecidos
• País: BR
• Palavras-chave em Português: Abelha; Mitose; Morte celular; Proteases; Intestino médio
• Palavras-chave em Inglês: Bee; Mitosis; Cell death; Proteases; Bowel average
• Área do conhecimento CNPq: CNPQ::CIENCIAS BIOLOGICAS::BIOLOGIA GERAL
• Link de acesso: http://locus.ufv.br/handle/123456789/259

Bees are holometabolus insects, since they present complete metamorphosis. During the metamorphosis of the insects the gut is completely remodeled. Few studies emphasize aspects of the renewal of the epithelium of bee s midgut during metamorphosis. Studies that describe digestive cell proliferation are lacking in images of mitosis. Little is known also about the changing enzyme that occurs in bees during this period. The genome of A. mellifera has recently been sequenced and several serine proteases genes identified. However, only a trypsin-like enzyme was purified and biochemically characterized in adult workers. This research is a contribution to the understanding of Hymenoptera biology in relation to some aspects of digestive physiology during metamorphosis of social insects. We proved that increasing the number of cells and the restoration of regenerative cell nests at the end of metamorphosis is a consequence of cell divisions in the black eyed pupae. The proliferation that occurs at this stage plays an important role in the renewal of the digestive cells of adult bees. We also observed that, during metamorphosis, death of the midgut epithelial cells of M. quadrifasciata anthidioides occurs by apoptosis and is accompanied by autophagy. Furthermore, this study observed different trypsin-like enzymes in the midgut from larvae and adults of A. mellifera.