Análise de Helicobacter pylori na placa dental e saliva e biópsia gástrica
Ano de defesa: | 2004 |
---|---|
Autor(a) principal: | |
Orientador(a): | , |
Banca de defesa: | |
Tipo de documento: | Dissertação |
Tipo de acesso: | Acesso aberto |
Idioma: | por |
Instituição de defesa: |
Universidade do Grande Rio
|
Programa de Pós-Graduação: |
Programa de Pós-Graduação em Odontologia
|
Departamento: |
Unigranrio::Odontologia
|
País: |
Brasil
|
Palavras-chave em Português: | |
Área do conhecimento CNPq: | |
Link de acesso: | http://localhost:8080/tede/handle/tede/36 |
Resumo: | Helicobacter pylori is a gram-negative bacterium, mobile, microaerophilic, associated with chronic gastritis, peptic ulcers, duodenal ulcers and consists of a risk factor for gastric cancer. The objective of this study was to evaluate the presence Helicobacter pylori in gastric biopsy samples, dental plaque and Saliva samples are collected from 26 subjects in this study were be examined by endoscopy. The colonization of this bacterium in biopsy Gastric was assessed by using three detection methods: histological analysis, rapid urease test and PCR method. For the detection of Helicobacter pylori Dental plaque and saliva was used the PCR method. In the PCR reaction was used a pair of synthetic primer-gene 16S r-RNA Helicobacter pylori: the HP1 primer: 5'-TGGAATCAGCGTCAGGTAATG-3 'and primer HP2: 5'- GCTAAGAGATCAGCCTATGTCC -3 '. The PCR amplification product was detected by electrophoresis on 1.5% agarose gel. First, it performed gastroscopy which ranked the 26 patients into two groups: 20 with gastric inflammation (pangastritis) and 6 considered normal. Then, the 20 Gastric biopsies of subjects with pangastritis were subjected to the three Helicobacter pylori detection methods selected for this study. THE Histopathological analysis identified 15 positive individuals Helicobacter pylori (75%, 15/20), rapid urease test identified 14 individuals (70%, 14/20) and PCR method identified 17 patients (85%, 17/20). The samples of dental plaque and the saliva, by PCR method suggests the absence of the bacterium Helicobacter pylori. Results from Helicobacter pylori detection methods in gastric samples from 6 subjects with no gastric inflammation resulted in a 17% (1/6) for histopathology, 33% (2/6) for the rapid urease test and 67% (4/6) for the PCR method. The PCR analysis in samples of dental plaque and saliva of these 6 patients suggests the absence of Helicobacter pylori. The presence of Helicobacter pylori was detected in gastric biopsies by histological methods, rapid urease test and PCR, the latter method had a higher sensitivity. The PCR analysis in dental plaque and saliva showed no presence of H. pylori in the population studied. |
id |
UGRI_d6e00f2104fec2afbd81dc29731467f8 |
---|---|
oai_identifier_str |
oai:localhost:tede/36 |
network_acronym_str |
UGRI |
network_name_str |
Biblioteca Digital de Teses e Dissertações da UNIGRANRIO |
repository_id_str |
|
spelling |
Tinoco, Eduardo M. B.Teixeira, Henrique G.C.Monteiro, Rosimere dos Santos Bastos2016-06-14T22:34:17Z2004-12-16Monteiro, Rosimere dos Santos Bastos. Análise de Helicobacter pylori na placa dental e saliva e biópsia gástrica. 2004. 48 f. Dissertação( Programa de Pós-Graduação em Odontologia) - Universidade do Grande Rio, Duque de Caxias.http://localhost:8080/tede/handle/tede/36Helicobacter pylori is a gram-negative bacterium, mobile, microaerophilic, associated with chronic gastritis, peptic ulcers, duodenal ulcers and consists of a risk factor for gastric cancer. The objective of this study was to evaluate the presence Helicobacter pylori in gastric biopsy samples, dental plaque and Saliva samples are collected from 26 subjects in this study were be examined by endoscopy. The colonization of this bacterium in biopsy Gastric was assessed by using three detection methods: histological analysis, rapid urease test and PCR method. For the detection of Helicobacter pylori Dental plaque and saliva was used the PCR method. In the PCR reaction was used a pair of synthetic primer-gene 16S r-RNA Helicobacter pylori: the HP1 primer: 5'-TGGAATCAGCGTCAGGTAATG-3 'and primer HP2: 5'- GCTAAGAGATCAGCCTATGTCC -3 '. The PCR amplification product was detected by electrophoresis on 1.5% agarose gel. First, it performed gastroscopy which ranked the 26 patients into two groups: 20 with gastric inflammation (pangastritis) and 6 considered normal. Then, the 20 Gastric biopsies of subjects with pangastritis were subjected to the three Helicobacter pylori detection methods selected for this study. THE Histopathological analysis identified 15 positive individuals Helicobacter pylori (75%, 15/20), rapid urease test identified 14 individuals (70%, 14/20) and PCR method identified 17 patients (85%, 17/20). The samples of dental plaque and the saliva, by PCR method suggests the absence of the bacterium Helicobacter pylori. Results from Helicobacter pylori detection methods in gastric samples from 6 subjects with no gastric inflammation resulted in a 17% (1/6) for histopathology, 33% (2/6) for the rapid urease test and 67% (4/6) for the PCR method. The PCR analysis in samples of dental plaque and saliva of these 6 patients suggests the absence of Helicobacter pylori. The presence of Helicobacter pylori was detected in gastric biopsies by histological methods, rapid urease test and PCR, the latter method had a higher sensitivity. The PCR analysis in dental plaque and saliva showed no presence of H. pylori in the population studied.Helicobacter pylori é uma bactéria gram-negativa, móvel, microaerofílica, associada à gastrite crônica, úlceras pépticas, úlceras duodenais e consiste em um fator de risco para o câncer gástrico. O objetivo deste trabalho foi avaliar a presença de Helicobacter pylori em amostras de biópsias gástricas, na placa dental e na saliva, sendo coletadas amostras de 26 indivíduos neste estudo que foram submetidos ao exame de endoscopia. A colonização desta bactéria na biópsia gástrica foi avaliada pelo uso de três métodos de detecção: a análise histológica, o teste rápido de urease e o método de PCR. Para detecção do Helicobacter pylori na placa dental e na saliva foi utilizado o método de PCR. Na reação de PCR foi utilizado um par de primer sintético do gen 16S-r-RNA do Helicobacter pylori: o primer HP1: 5’-TGGAATCAGCGTCAGGTAATG-3’ e o primer HP2: 5’- GCTAAGAGATCAGCCTATGTCC -3’. A amplificação dos produtos por PCR foi detectada por eletroforese em gel de agarose 1,5%. Primeiramente, foi realizada endoscopia gástrica que classificou os 26 pacientes em dois grupos: 20 com inflamação gástrica (pangastrite) e 6 considerados normais. Em seguida, as 20 biópsias gástricas dos indivíduos com pangastrite foram submetidos aos três métodos de detecção de Helicobacter pylori selecionados para este estudo. A análise histopatológica identificou 15 indivíduos positivos para Helicobacter pylori (75%, 15/20), o teste rápido de urease identificou 14 indivíduos (70%, 14/20) e o método de PCR identificou 17 indivíduos (85%, 17/20). As amostras da placa dental e da saliva destes pacientes, pelo método de PCR, sugerem a ausência da bactéria Helicobacter pylori. Os resultados dos métodos de detecção de Helicobacter pylori nas amostras gástricas dos 6 indivíduos sem inflamação gástrica resultaram em 17% (1/6) para o exame histopatológico, 33% (2/6) para o teste rápido de urease e 67% (4/6) para o método de PCR. A análise de PCR nas amostras de placa dental e de saliva destes 6 pacientes sugere a ausência de Helicobacter pylori. A presença de Helicobacter pylori foi detectada nas biópsias gástricas pelos métodos de histologia, teste rápido de urease e PCR, sendo que este último método apresentou uma maior sensibilidade. A análise de PCR na placa dental e na saliva não evidenciou a presença de H.pylori na população estudada.Submitted by Patricia Vieira Silva (patricia.silva@unigranrio.com.br) on 2016-06-14T22:34:17Z No. of bitstreams: 1 Rosimere dos Santos Bastos Monteiro.pdf: 225581 bytes, checksum: db0d380ebe424c863b68d1b49c50975a (MD5)Made available in DSpace on 2016-06-14T22:34:17Z (GMT). No. of bitstreams: 1 Rosimere dos Santos Bastos Monteiro.pdf: 225581 bytes, checksum: db0d380ebe424c863b68d1b49c50975a (MD5) Previous issue date: 2004-12-16application/pdfporUniversidade do Grande RioPrograma de Pós-Graduação em OdontologiaUNIGRANRIOBrasilUnigranrio::OdontologiaOdontologiaPeriodontiaHelicobacter pyloriTécnicas de diagnóstico e procedimentosEstômago - Doenças - DiagnósticoPlacas dentárias - MicrobiologiaSaliva - MicrobiologiaODONTOLOGIA::PERIODONTIAAnálise de Helicobacter pylori na placa dental e saliva e biópsia gástricainfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesis-80965548187336651645005006008586829874625042951-6742610990284825032info:eu-repo/semantics/openAccessreponame:Biblioteca Digital de Teses e Dissertações da UNIGRANRIOinstname:Universidade do Grande Rio (UNIGRANRIO)instacron:UNIGRANRIOLICENSElicense.txtlicense.txttext/plain; charset=utf-81982http://localhost:8080/tede/bitstream/tede/36/1/license.txt4a50535e8405f611398e6e2d408dbd1bMD51ORIGINALRosimere dos Santos Bastos Monteiro.pdfRosimere dos Santos Bastos Monteiro.pdfapplication/pdf225581http://localhost:8080/tede/bitstream/tede/36/2/Rosimere+dos+Santos+Bastos+Monteiro.pdfdb0d380ebe424c863b68d1b49c50975aMD52tede/362016-06-14 19:34:17.894oai:localhost: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Biblioteca Digital de Teses e Dissertaçõeshttp://tede.unigranrio.edu.br/PUBhttp://tede.unigranrio.edu.br/oai/requestrepositorio@instituicao.br||repositorio@instituicao.bropendoar:2016-06-14T22:34:17Biblioteca Digital de Teses e Dissertações da UNIGRANRIO - Universidade do Grande Rio (UNIGRANRIO)false |
dc.title.por.fl_str_mv |
Análise de Helicobacter pylori na placa dental e saliva e biópsia gástrica |
title |
Análise de Helicobacter pylori na placa dental e saliva e biópsia gástrica |
spellingShingle |
Análise de Helicobacter pylori na placa dental e saliva e biópsia gástrica Monteiro, Rosimere dos Santos Bastos Odontologia Periodontia Helicobacter pylori Técnicas de diagnóstico e procedimentos Estômago - Doenças - Diagnóstico Placas dentárias - Microbiologia Saliva - Microbiologia ODONTOLOGIA::PERIODONTIA |
title_short |
Análise de Helicobacter pylori na placa dental e saliva e biópsia gástrica |
title_full |
Análise de Helicobacter pylori na placa dental e saliva e biópsia gástrica |
title_fullStr |
Análise de Helicobacter pylori na placa dental e saliva e biópsia gástrica |
title_full_unstemmed |
Análise de Helicobacter pylori na placa dental e saliva e biópsia gástrica |
title_sort |
Análise de Helicobacter pylori na placa dental e saliva e biópsia gástrica |
author |
Monteiro, Rosimere dos Santos Bastos |
author_facet |
Monteiro, Rosimere dos Santos Bastos |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Tinoco, Eduardo M. B. |
dc.contributor.advisor2.fl_str_mv |
Teixeira, Henrique G.C. |
dc.contributor.author.fl_str_mv |
Monteiro, Rosimere dos Santos Bastos |
contributor_str_mv |
Tinoco, Eduardo M. B. Teixeira, Henrique G.C. |
dc.subject.por.fl_str_mv |
Odontologia Periodontia Helicobacter pylori Técnicas de diagnóstico e procedimentos Estômago - Doenças - Diagnóstico Placas dentárias - Microbiologia Saliva - Microbiologia |
topic |
Odontologia Periodontia Helicobacter pylori Técnicas de diagnóstico e procedimentos Estômago - Doenças - Diagnóstico Placas dentárias - Microbiologia Saliva - Microbiologia ODONTOLOGIA::PERIODONTIA |
dc.subject.cnpq.fl_str_mv |
ODONTOLOGIA::PERIODONTIA |
description |
Helicobacter pylori is a gram-negative bacterium, mobile, microaerophilic, associated with chronic gastritis, peptic ulcers, duodenal ulcers and consists of a risk factor for gastric cancer. The objective of this study was to evaluate the presence Helicobacter pylori in gastric biopsy samples, dental plaque and Saliva samples are collected from 26 subjects in this study were be examined by endoscopy. The colonization of this bacterium in biopsy Gastric was assessed by using three detection methods: histological analysis, rapid urease test and PCR method. For the detection of Helicobacter pylori Dental plaque and saliva was used the PCR method. In the PCR reaction was used a pair of synthetic primer-gene 16S r-RNA Helicobacter pylori: the HP1 primer: 5'-TGGAATCAGCGTCAGGTAATG-3 'and primer HP2: 5'- GCTAAGAGATCAGCCTATGTCC -3 '. The PCR amplification product was detected by electrophoresis on 1.5% agarose gel. First, it performed gastroscopy which ranked the 26 patients into two groups: 20 with gastric inflammation (pangastritis) and 6 considered normal. Then, the 20 Gastric biopsies of subjects with pangastritis were subjected to the three Helicobacter pylori detection methods selected for this study. THE Histopathological analysis identified 15 positive individuals Helicobacter pylori (75%, 15/20), rapid urease test identified 14 individuals (70%, 14/20) and PCR method identified 17 patients (85%, 17/20). The samples of dental plaque and the saliva, by PCR method suggests the absence of the bacterium Helicobacter pylori. Results from Helicobacter pylori detection methods in gastric samples from 6 subjects with no gastric inflammation resulted in a 17% (1/6) for histopathology, 33% (2/6) for the rapid urease test and 67% (4/6) for the PCR method. The PCR analysis in samples of dental plaque and saliva of these 6 patients suggests the absence of Helicobacter pylori. The presence of Helicobacter pylori was detected in gastric biopsies by histological methods, rapid urease test and PCR, the latter method had a higher sensitivity. The PCR analysis in dental plaque and saliva showed no presence of H. pylori in the population studied. |
publishDate |
2004 |
dc.date.issued.fl_str_mv |
2004-12-16 |
dc.date.accessioned.fl_str_mv |
2016-06-14T22:34:17Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
Monteiro, Rosimere dos Santos Bastos. Análise de Helicobacter pylori na placa dental e saliva e biópsia gástrica. 2004. 48 f. Dissertação( Programa de Pós-Graduação em Odontologia) - Universidade do Grande Rio, Duque de Caxias. |
dc.identifier.uri.fl_str_mv |
http://localhost:8080/tede/handle/tede/36 |
identifier_str_mv |
Monteiro, Rosimere dos Santos Bastos. Análise de Helicobacter pylori na placa dental e saliva e biópsia gástrica. 2004. 48 f. Dissertação( Programa de Pós-Graduação em Odontologia) - Universidade do Grande Rio, Duque de Caxias. |
url |
http://localhost:8080/tede/handle/tede/36 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.program.fl_str_mv |
-8096554818733665164 |
dc.relation.confidence.fl_str_mv |
500 500 600 |
dc.relation.department.fl_str_mv |
8586829874625042951 |
dc.relation.cnpq.fl_str_mv |
-6742610990284825032 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade do Grande Rio |
dc.publisher.program.fl_str_mv |
Programa de Pós-Graduação em Odontologia |
dc.publisher.initials.fl_str_mv |
UNIGRANRIO |
dc.publisher.country.fl_str_mv |
Brasil |
dc.publisher.department.fl_str_mv |
Unigranrio::Odontologia |
publisher.none.fl_str_mv |
Universidade do Grande Rio |
dc.source.none.fl_str_mv |
reponame:Biblioteca Digital de Teses e Dissertações da UNIGRANRIO instname:Universidade do Grande Rio (UNIGRANRIO) instacron:UNIGRANRIO |
instname_str |
Universidade do Grande Rio (UNIGRANRIO) |
instacron_str |
UNIGRANRIO |
institution |
UNIGRANRIO |
reponame_str |
Biblioteca Digital de Teses e Dissertações da UNIGRANRIO |
collection |
Biblioteca Digital de Teses e Dissertações da UNIGRANRIO |
bitstream.url.fl_str_mv |
http://localhost:8080/tede/bitstream/tede/36/1/license.txt http://localhost:8080/tede/bitstream/tede/36/2/Rosimere+dos+Santos+Bastos+Monteiro.pdf |
bitstream.checksum.fl_str_mv |
4a50535e8405f611398e6e2d408dbd1b db0d380ebe424c863b68d1b49c50975a |
bitstream.checksumAlgorithm.fl_str_mv |
MD5 MD5 |
repository.name.fl_str_mv |
Biblioteca Digital de Teses e Dissertações da UNIGRANRIO - Universidade do Grande Rio (UNIGRANRIO) |
repository.mail.fl_str_mv |
repositorio@instituicao.br||repositorio@instituicao.br |
_version_ |
1797240305127260160 |