Estudo morfológico e morfométrico das fibras musculares e junções neuromusculares do músculo extensor longo dos dedos de ratos cujas mães foram submetidas à restrição proteica
Ano de defesa: | 2013 |
---|---|
Autor(a) principal: | |
Orientador(a): | |
Banca de defesa: | |
Tipo de documento: | Dissertação |
Tipo de acesso: | Acesso aberto |
Idioma: | por |
Instituição de defesa: |
Universidade Estadual do Oeste do Parana
|
Programa de Pós-Graduação: |
Programa de Pós-Graduação Stricto Sensu em Biociências e Saúde
|
Departamento: |
Biologia, processo saúde-doença e políticas da saúde
|
País: |
BR
|
Palavras-chave em Português: | |
Palavras-chave em Inglês: | |
Área do conhecimento CNPq: | |
Link de acesso: | http://tede.unioeste.br:8080/tede/handle/tede/638 |
Resumo: | Protein energy malnutrition causes changes in several organs and systems and the earlier malnutrition occurs, more intense and permanent will be its effects. This study aimed to evaluate the morphological and morphometric aspects of the muscle fibers and neuromuscular junctions (end-plates) of the extensor digitorum longus (EDL) of rats subjected to maternal protein restriction during the periods of gestation and lactation. Wistar rats were divided into four groups: 1) control group 21 days - the offspring of mothers fed during gestation and lactation a normal protein diet (17 %); 2) restricted group 21 days - the offspring of mothers fed during gestation and lactation with a low protein diet (6 %); 3) control group 365 days - the offspring of mothers fed during gestation and lactation on a normal protein diet (17 %) until the day of weaning, and after this period the diet was standard diet until age 365 days; and 4) restricted group 365 days - the offspring of mothers fed during gestation and lactation with a low protein diet (6 %) until the day of weaning, and after this period the diet was standard diet until age 365 days. After the periods of 21 and 365 days, the male rats of each group were weighed and euthanized for the removal of the EDL muscle. The hematoxylin-eosin (HE) technique was performed for the histological analysis of the muscle fibers and histoenzymological analysis was performed using nicotinamide adenine dinucleotide tetrazolium reductase (NADH - TR). The ultrastructural study of the EDL muscle fibers was conducted using a transmission electron microscope. For the end-plates staining, muscle samples were subjected to nonspecific esterase reaction. Restricted animals with 21 or 365 days old showed a significant decrease in body weight. Histological analysis of the fibers in the restricted animals aged 21 days showed an increased amount of fetal fibers, myotubes and centered nuclei, compared with the respective control group. Histoenzymological analysis revealed a significant decrease in the area of type IIa fibers in the animals of the restricted group aged 365 days compared with the control group of the same age. Regarding the ultrastructural study, the EDL muscle of restricted animals with 21 days old showed dissolution of the Z line, the presence of lipid droplets in the midst of myofibrils, myelin figures in the subsarcolemmal and intramiofibrilar regions and nuclei in the central position in the muscle fiber line were frequent. Disorganization of the Z line and the myofibrils was also found in animals from the restricted group aged 365 days. The analysis of the end-plates showed a significant decrease in the area, and in the major and minor diameters of the restricted animals aged 21 days compared to their respective controls. These results suggest that maternal protein restriction during gestation and lactation alters the development and morphology of the offspring skeletal muscle |
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Torrejais, Marcia MirandaCPF:81287364934http://lattes.cnpq.br/6079757636423339CPF:28416529809http://lattes.cnpq.br/5560332676203402Jeronimo, Léslie Cazetta2017-07-10T14:17:05Z2014-06-262013-08-16JERONIMO, Léslie Cazetta. Morphological and morphometric study of muscle fibers and neuromuscular junctions of the extensor digitorum rats whose mothers were submitted to protein restriction. 2013. 64 f. Dissertação (Mestrado em Biologia, processo saúde-doença e políticas da saúde) - Universidade Estadual do Oeste do Parana, Cascavel, 2013.http://tede.unioeste.br:8080/tede/handle/tede/638Protein energy malnutrition causes changes in several organs and systems and the earlier malnutrition occurs, more intense and permanent will be its effects. This study aimed to evaluate the morphological and morphometric aspects of the muscle fibers and neuromuscular junctions (end-plates) of the extensor digitorum longus (EDL) of rats subjected to maternal protein restriction during the periods of gestation and lactation. Wistar rats were divided into four groups: 1) control group 21 days - the offspring of mothers fed during gestation and lactation a normal protein diet (17 %); 2) restricted group 21 days - the offspring of mothers fed during gestation and lactation with a low protein diet (6 %); 3) control group 365 days - the offspring of mothers fed during gestation and lactation on a normal protein diet (17 %) until the day of weaning, and after this period the diet was standard diet until age 365 days; and 4) restricted group 365 days - the offspring of mothers fed during gestation and lactation with a low protein diet (6 %) until the day of weaning, and after this period the diet was standard diet until age 365 days. After the periods of 21 and 365 days, the male rats of each group were weighed and euthanized for the removal of the EDL muscle. The hematoxylin-eosin (HE) technique was performed for the histological analysis of the muscle fibers and histoenzymological analysis was performed using nicotinamide adenine dinucleotide tetrazolium reductase (NADH - TR). The ultrastructural study of the EDL muscle fibers was conducted using a transmission electron microscope. For the end-plates staining, muscle samples were subjected to nonspecific esterase reaction. Restricted animals with 21 or 365 days old showed a significant decrease in body weight. Histological analysis of the fibers in the restricted animals aged 21 days showed an increased amount of fetal fibers, myotubes and centered nuclei, compared with the respective control group. Histoenzymological analysis revealed a significant decrease in the area of type IIa fibers in the animals of the restricted group aged 365 days compared with the control group of the same age. Regarding the ultrastructural study, the EDL muscle of restricted animals with 21 days old showed dissolution of the Z line, the presence of lipid droplets in the midst of myofibrils, myelin figures in the subsarcolemmal and intramiofibrilar regions and nuclei in the central position in the muscle fiber line were frequent. Disorganization of the Z line and the myofibrils was also found in animals from the restricted group aged 365 days. The analysis of the end-plates showed a significant decrease in the area, and in the major and minor diameters of the restricted animals aged 21 days compared to their respective controls. These results suggest that maternal protein restriction during gestation and lactation alters the development and morphology of the offspring skeletal muscleA desnutrição energético proteica (DEP) causa alterações em diversos sistemas e órgãos, sendo seus efeitos mais intensos e permanentes quanto mais precocemente ocorrer a desnutrição. O presente trabalho buscou avaliar os aspectos morfológicos e morfométricos das fibras musculares e junções neuromusculares (JNMs) do músculo extensor longo dos dedos (EDL) de ratos submetidos à restrição proteica materna, durante os períodos de gestação e lactação. Ratos da linhagem Wistar foram divididos em quatro grupos: Grupo controle 21 dias - prole de mães alimentadas durante a gestação e lactação com ração normoproteica (17%); Grupo restrito 21 dias - prole de mães alimentadas durante a gestação e lactação com ração hipoproteica (6%); Grupo controle 365 dias - prole de mães alimentadas durante a gestação e lactação com ração normoproteica (17%) até o dia do desmame e, após este período, a dieta fornecida para a prole foi ração normoproteica até a idade de 365 dias e Grupo restrito 365 dias - prole de mães alimentadas durante a gestação e lactação com ração hipoproteica (6%) até o dia do desmame e, após este período, a dieta fornecida foi ração normoproteica até a idade de 365 dias. Após os períodos 21 e 365 dias, os ratos machos de cada grupo foram pesados e eutanasiados para a retirada do músculo EDL. Para o estudo histológico das fibras musculares foi realizado a técnica Hematoxilina-Eosina (HE) e para análise histoenzimológica foi feita a marcação com o Nicotinamida Adenina Dinucleotídeo - Tetrazolium Reductase - (NADH-TR). O estudo ultraestrutural das fibras musculares do EDL foi conduzido no microscópio eletrônico de transmissão. Para a marcação das JNMs, amostras do músculo foram submetidas à reação Esterase Inespecífica. Os resultados mostraram diminuição do peso corpóreo nos animais restritos com 21 e 365 dias de idade. A análise histológica das fibras musculares nos animais restrito com 21 dias mostrou maior quantidade de fibras fetais, miotubos e núcleos centralizados, quando comparado com seu respectivo grupo controle. O estudo histoenzimológico revelou uma diminuição significativa da área das fibras tipo IIa nos animais do grupo restrito 365 dias de idade em relação ao grupo controle de mesma idade. Quanto ao estudo ultraestrutural, o músculo EDL nos animais do grupo restrito 21 dias apresentou dissolução da linha Z, presença de gotículas de lipídios em meio às miofibrilas e figuras de mielina nas regiões subsarcolemal e intramiofibrilar e núcleos em posição central na fibra muscular foram frequentes. No grupo restrito 365 dias também foi verificado uma desorganização da linha Z e das miofibrilas. A análise das JNMs mostrou que houve diminuição significativa da área e dos diâmetros maior e menor nos animais restritos com 21 dias quando comparado com seu respectivo grupo controle. Esses resultados sugerem que restrição proteica materna durante a gestação e lactação altera o desenvolvimento e morfologia do músculo estriado esquelético da proleMade available in DSpace on 2017-07-10T14:17:05Z (GMT). No. of bitstreams: 1 Leslie Jeronimo.pdf: 1310262 bytes, checksum: 31b9beed076ffaa86c2f666ef6e3d8f6 (MD5) Previous issue date: 2013-08-16application/pdfporUniversidade Estadual do Oeste do ParanaPrograma de Pós-Graduação Stricto Sensu em Biociências e SaúdeUNIOESTEBRBiologia, processo saúde-doença e políticas da saúdemorfologiamorfometriarestrição proteicamúsculo extensor longo dos dedosratomorphologymorphometryprotein restrictionextensor digitorum longus muscleratCNPQ::CIENCIAS DA SAUDEEstudo morfológico e morfométrico das fibras musculares e junções neuromusculares do músculo extensor longo dos dedos de ratos cujas mães foram submetidas à restrição proteicaMorphological and morphometric study of muscle fibers and neuromuscular junctions of the extensor digitorum rats whose mothers were submitted to protein restrictioninfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisinfo:eu-repo/semantics/openAccessreponame:Biblioteca Digital de Teses e Dissertações do UNIOESTEinstname:Universidade Estadual do Oeste do Paraná (UNIOESTE)instacron:UNIOESTEORIGINALLeslie Jeronimo.pdfapplication/pdf1310262http://tede.unioeste.br:8080/tede/bitstream/tede/638/1/Leslie+Jeronimo.pdf31b9beed076ffaa86c2f666ef6e3d8f6MD51tede/6382017-07-10 11:17:05.906oai:tede.unioeste.br:tede/638Biblioteca Digital de Teses e Dissertaçõeshttp://tede.unioeste.br/PUBhttp://tede.unioeste.br/oai/requestbiblioteca.repositorio@unioeste.bropendoar:2017-07-10T14:17:05Biblioteca Digital de Teses e Dissertações do UNIOESTE - Universidade Estadual do Oeste do Paraná (UNIOESTE)false |
dc.title.por.fl_str_mv |
Estudo morfológico e morfométrico das fibras musculares e junções neuromusculares do músculo extensor longo dos dedos de ratos cujas mães foram submetidas à restrição proteica |
dc.title.alternative.eng.fl_str_mv |
Morphological and morphometric study of muscle fibers and neuromuscular junctions of the extensor digitorum rats whose mothers were submitted to protein restriction |
title |
Estudo morfológico e morfométrico das fibras musculares e junções neuromusculares do músculo extensor longo dos dedos de ratos cujas mães foram submetidas à restrição proteica |
spellingShingle |
Estudo morfológico e morfométrico das fibras musculares e junções neuromusculares do músculo extensor longo dos dedos de ratos cujas mães foram submetidas à restrição proteica Jeronimo, Léslie Cazetta morfologia morfometria restrição proteica músculo extensor longo dos dedos rato morphology morphometry protein restriction extensor digitorum longus muscle rat CNPQ::CIENCIAS DA SAUDE |
title_short |
Estudo morfológico e morfométrico das fibras musculares e junções neuromusculares do músculo extensor longo dos dedos de ratos cujas mães foram submetidas à restrição proteica |
title_full |
Estudo morfológico e morfométrico das fibras musculares e junções neuromusculares do músculo extensor longo dos dedos de ratos cujas mães foram submetidas à restrição proteica |
title_fullStr |
Estudo morfológico e morfométrico das fibras musculares e junções neuromusculares do músculo extensor longo dos dedos de ratos cujas mães foram submetidas à restrição proteica |
title_full_unstemmed |
Estudo morfológico e morfométrico das fibras musculares e junções neuromusculares do músculo extensor longo dos dedos de ratos cujas mães foram submetidas à restrição proteica |
title_sort |
Estudo morfológico e morfométrico das fibras musculares e junções neuromusculares do músculo extensor longo dos dedos de ratos cujas mães foram submetidas à restrição proteica |
author |
Jeronimo, Léslie Cazetta |
author_facet |
Jeronimo, Léslie Cazetta |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Torrejais, Marcia Miranda |
dc.contributor.advisor1ID.fl_str_mv |
CPF:81287364934 |
dc.contributor.advisor1Lattes.fl_str_mv |
http://lattes.cnpq.br/6079757636423339 |
dc.contributor.authorID.fl_str_mv |
CPF:28416529809 |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/5560332676203402 |
dc.contributor.author.fl_str_mv |
Jeronimo, Léslie Cazetta |
contributor_str_mv |
Torrejais, Marcia Miranda |
dc.subject.por.fl_str_mv |
morfologia morfometria restrição proteica músculo extensor longo dos dedos rato |
topic |
morfologia morfometria restrição proteica músculo extensor longo dos dedos rato morphology morphometry protein restriction extensor digitorum longus muscle rat CNPQ::CIENCIAS DA SAUDE |
dc.subject.eng.fl_str_mv |
morphology morphometry protein restriction extensor digitorum longus muscle rat |
dc.subject.cnpq.fl_str_mv |
CNPQ::CIENCIAS DA SAUDE |
description |
Protein energy malnutrition causes changes in several organs and systems and the earlier malnutrition occurs, more intense and permanent will be its effects. This study aimed to evaluate the morphological and morphometric aspects of the muscle fibers and neuromuscular junctions (end-plates) of the extensor digitorum longus (EDL) of rats subjected to maternal protein restriction during the periods of gestation and lactation. Wistar rats were divided into four groups: 1) control group 21 days - the offspring of mothers fed during gestation and lactation a normal protein diet (17 %); 2) restricted group 21 days - the offspring of mothers fed during gestation and lactation with a low protein diet (6 %); 3) control group 365 days - the offspring of mothers fed during gestation and lactation on a normal protein diet (17 %) until the day of weaning, and after this period the diet was standard diet until age 365 days; and 4) restricted group 365 days - the offspring of mothers fed during gestation and lactation with a low protein diet (6 %) until the day of weaning, and after this period the diet was standard diet until age 365 days. After the periods of 21 and 365 days, the male rats of each group were weighed and euthanized for the removal of the EDL muscle. The hematoxylin-eosin (HE) technique was performed for the histological analysis of the muscle fibers and histoenzymological analysis was performed using nicotinamide adenine dinucleotide tetrazolium reductase (NADH - TR). The ultrastructural study of the EDL muscle fibers was conducted using a transmission electron microscope. For the end-plates staining, muscle samples were subjected to nonspecific esterase reaction. Restricted animals with 21 or 365 days old showed a significant decrease in body weight. Histological analysis of the fibers in the restricted animals aged 21 days showed an increased amount of fetal fibers, myotubes and centered nuclei, compared with the respective control group. Histoenzymological analysis revealed a significant decrease in the area of type IIa fibers in the animals of the restricted group aged 365 days compared with the control group of the same age. Regarding the ultrastructural study, the EDL muscle of restricted animals with 21 days old showed dissolution of the Z line, the presence of lipid droplets in the midst of myofibrils, myelin figures in the subsarcolemmal and intramiofibrilar regions and nuclei in the central position in the muscle fiber line were frequent. Disorganization of the Z line and the myofibrils was also found in animals from the restricted group aged 365 days. The analysis of the end-plates showed a significant decrease in the area, and in the major and minor diameters of the restricted animals aged 21 days compared to their respective controls. These results suggest that maternal protein restriction during gestation and lactation alters the development and morphology of the offspring skeletal muscle |
publishDate |
2013 |
dc.date.issued.fl_str_mv |
2013-08-16 |
dc.date.available.fl_str_mv |
2014-06-26 |
dc.date.accessioned.fl_str_mv |
2017-07-10T14:17:05Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
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masterThesis |
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publishedVersion |
dc.identifier.citation.fl_str_mv |
JERONIMO, Léslie Cazetta. Morphological and morphometric study of muscle fibers and neuromuscular junctions of the extensor digitorum rats whose mothers were submitted to protein restriction. 2013. 64 f. Dissertação (Mestrado em Biologia, processo saúde-doença e políticas da saúde) - Universidade Estadual do Oeste do Parana, Cascavel, 2013. |
dc.identifier.uri.fl_str_mv |
http://tede.unioeste.br:8080/tede/handle/tede/638 |
identifier_str_mv |
JERONIMO, Léslie Cazetta. Morphological and morphometric study of muscle fibers and neuromuscular junctions of the extensor digitorum rats whose mothers were submitted to protein restriction. 2013. 64 f. Dissertação (Mestrado em Biologia, processo saúde-doença e políticas da saúde) - Universidade Estadual do Oeste do Parana, Cascavel, 2013. |
url |
http://tede.unioeste.br:8080/tede/handle/tede/638 |
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por |
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Universidade Estadual do Oeste do Parana |
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Programa de Pós-Graduação Stricto Sensu em Biociências e Saúde |
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UNIOESTE |
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BR |
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Biologia, processo saúde-doença e políticas da saúde |
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Universidade Estadual do Oeste do Parana |
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