Qualidade e segurança alimentar do milho em diferentes acondicionamentos de armazenagem

Detalhes bibliográficos
Ano de defesa: 2014
Autor(a) principal: Domenico, Adriana Sbardelotto Di lattes
Orientador(a): Christ, Divair lattes
Banca de defesa: Coelho, Silvia Renata Machado lattes, Frata, Marcela Tostes lattes, Sousa, Fernando Carlos de lattes, Busso, Cleverson lattes
Tipo de documento: Tese
Tipo de acesso: Acesso aberto
Idioma: por
Instituição de defesa: Universidade Estadual do Oeste do Parana
Programa de Pós-Graduação: Programa de Pós-Graduação "Stricto Sensu" em Engenharia Agrícola
Departamento: Engenharia
País: BR
Palavras-chave em Português:
Palavras-chave em Inglês:
Área do conhecimento CNPq:
Link de acesso: http://tede.unioeste.br:8080/tede/handle/tede/2658
Resumo: Brazil is one of the largest producers of grains, and maize is the second most produced grain in the country. As this cereal is directly related to human and animal food basis, many matters appear on the maintenance of their post-harvest quality, especially during storage, due to the increased attention to food safety. Despite this importance, maize is often stored on farms in inappropriate conditions, exposed to quantitative and qualitative losses, the development of toxigenic fungi and aflatoxin contamination, a substance capable of causing severe damage to human and animal health, as well as many agribusiness losses. The objective of this research was evaluating storage alternatives viable to farms through two storage experiments (2012 winter crop 2012/2013 summer harvests), for assessment of the quality of stored maize in different packings for 12 months. The experimental design was a 4 x 5 factorial, whose factors were four types of packaging storage (conventional sacks, bags hermetically sealed, metallic silo and cobs) and five time periods (0, 3, 6, 9 and 12 months). Two maize hybrids (2B688RR, 30K73Hx) cultivated in the region of the town of Dois Vizinhos were used, in the southwest of Paraná. The storage was conducted in ambient conditions and the following parameters were evaluated: water content, ash, protein, fat, grains without defects, volumetric weight, one-thousand grains weight, counting of Aspergillus sp, Fusarium sp, Penicillium sp, total of molds and yeasts and occurrence of aflatoxins (B1, B2, G1 and G2). The results obtained in each experiment, when attended the presuppositions of the mathematical model, were evaluated by use of analysis of variance and differences between means (p<0.05) by Tukey test at 5% significance level, and when not attended them, by Kruskal-Wallis also at 5% significance level. Furthermore, the Principal Component Analysis (PCA) was done to determine which of the variables evaluated was responsible for the largest variations in the quality of stored maize. It was found in both storages that maize kernels placed in sealed bags presented the lowest water content. Also that regardless of packaging and hybrid maize, there were an increase in ash content of grains and the decrease of the percentage of grains without defects and volumetric weight. It was detected the incidence of Aspergillus sp. Fusarium sp. and Penicillium sp., respectively 20.37, 86.11 and 94.44% of the samples of maize season winter storage, and in 83.3, 91.6 and 90.07% of the samples from the summer season storage. Aspergillus sp. was only detected after 3 months of storage in the first experiment, while in the second since harvesting. The occurrence of Fusarium sp. tended to decrease during storage, and Penicillium sp. was higher in corn conditioning in cobs. There was no influence of types of packaging and storage time on the occurrence of Aspergillus sp. and aflatoxins in maize season winter storage. However, the conditioning in maize cobs of season storage summer had the lowest count of Aspergillus sp. and did not present aflatoxin. Aflatoxins (B1+B2+G1+G2) detected ranged from 2.77 to 14.45 &#956;g kg-1 and from 3.03 to 197.51 &#956;g kg-1 respectively in the maize storage winter and summer. Besides this, none of the samples of the first storage showed contamination higher than 20 &#956;g kg-1, while in the second 41 samples presented higher values. The results are due to the different climatic conditions of the periods of cultivation of hybrid maize (summer and winter seasons) and storage experiments, the disparity in the incidence of Aspergillus sp. and aflatoxin contamination. The conclusion is that by ACP all variables assessed in this study are important for the quality of stored maize.
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spelling Christ, DivairCPF:66206863972http://lattes.cnpq.br/6200553304840204Coelho, Silvia Renata MachadoCPF:88213943600http://lattes.cnpq.br/3554106124561773Frata, Marcela TostesCPF:00335688985http://lattes.cnpq.br/8243438180476961Sousa, Fernando Carlos deCPF:02781191906http://lattes.cnpq.br/1004312837873706Busso, CleversonCPF:02755772956http://lattes.cnpq.br/5986131313813011CPF:0376609690http://lattes.cnpq.br/7513246316553733Domenico, Adriana Sbardelotto Di2017-07-10T19:23:52Z2015-08-252014-02-28DOMENICO, Adriana Sbardelotto Di. Food quality and safety of maize in different types of storage. 2014. 156 f. Tese (Doutorado em Engenharia) - Universidade Estadual do Oeste do Parana, Cascavel, 2014.http://tede.unioeste.br:8080/tede/handle/tede/2658Brazil is one of the largest producers of grains, and maize is the second most produced grain in the country. As this cereal is directly related to human and animal food basis, many matters appear on the maintenance of their post-harvest quality, especially during storage, due to the increased attention to food safety. Despite this importance, maize is often stored on farms in inappropriate conditions, exposed to quantitative and qualitative losses, the development of toxigenic fungi and aflatoxin contamination, a substance capable of causing severe damage to human and animal health, as well as many agribusiness losses. The objective of this research was evaluating storage alternatives viable to farms through two storage experiments (2012 winter crop 2012/2013 summer harvests), for assessment of the quality of stored maize in different packings for 12 months. The experimental design was a 4 x 5 factorial, whose factors were four types of packaging storage (conventional sacks, bags hermetically sealed, metallic silo and cobs) and five time periods (0, 3, 6, 9 and 12 months). Two maize hybrids (2B688RR, 30K73Hx) cultivated in the region of the town of Dois Vizinhos were used, in the southwest of Paraná. The storage was conducted in ambient conditions and the following parameters were evaluated: water content, ash, protein, fat, grains without defects, volumetric weight, one-thousand grains weight, counting of Aspergillus sp, Fusarium sp, Penicillium sp, total of molds and yeasts and occurrence of aflatoxins (B1, B2, G1 and G2). The results obtained in each experiment, when attended the presuppositions of the mathematical model, were evaluated by use of analysis of variance and differences between means (p<0.05) by Tukey test at 5% significance level, and when not attended them, by Kruskal-Wallis also at 5% significance level. Furthermore, the Principal Component Analysis (PCA) was done to determine which of the variables evaluated was responsible for the largest variations in the quality of stored maize. It was found in both storages that maize kernels placed in sealed bags presented the lowest water content. Also that regardless of packaging and hybrid maize, there were an increase in ash content of grains and the decrease of the percentage of grains without defects and volumetric weight. It was detected the incidence of Aspergillus sp. Fusarium sp. and Penicillium sp., respectively 20.37, 86.11 and 94.44% of the samples of maize season winter storage, and in 83.3, 91.6 and 90.07% of the samples from the summer season storage. Aspergillus sp. was only detected after 3 months of storage in the first experiment, while in the second since harvesting. The occurrence of Fusarium sp. tended to decrease during storage, and Penicillium sp. was higher in corn conditioning in cobs. There was no influence of types of packaging and storage time on the occurrence of Aspergillus sp. and aflatoxins in maize season winter storage. However, the conditioning in maize cobs of season storage summer had the lowest count of Aspergillus sp. and did not present aflatoxin. Aflatoxins (B1+B2+G1+G2) detected ranged from 2.77 to 14.45 &#956;g kg-1 and from 3.03 to 197.51 &#956;g kg-1 respectively in the maize storage winter and summer. Besides this, none of the samples of the first storage showed contamination higher than 20 &#956;g kg-1, while in the second 41 samples presented higher values. The results are due to the different climatic conditions of the periods of cultivation of hybrid maize (summer and winter seasons) and storage experiments, the disparity in the incidence of Aspergillus sp. and aflatoxin contamination. The conclusion is that by ACP all variables assessed in this study are important for the quality of stored maize.O Brasil é um dos maiores produtores mundiais de grãos e o milho é o segundo grão mais produzido no país. Como este cereal está diretamente relacionado à base alimentar humana e animal, surgem muitas preocupações a respeito da manutenção de sua qualidade no pós-colheita, em especial ao longo do armazenamento, com aumento das atenções à segurança alimentar. Apesar disso, muitas vezes o milho é armazenado nas propriedades rurais, em condições inadequadas, exposto a perdas quantitativas e qualitativas, ao desenvolvimento de fungos toxigênicos e à contaminação por aflatoxinas, substância capaz de causar graves danos à saúde humana e animal, além de inúmeros prejuízos agropecuários. Assim, o objetivo deste trabalho foi avaliar alternativas de armazenagem, viáveis a propriedades rurais, através de dois experimentos de armazenagem (safra de inverno 2012 e safra de verão 2012/2013), nos quais se avaliou a qualidade do milho armazenado em diferentes acondicionamentos, por 12 meses. O delineamento experimental utilizado foi um fatorial 4 x 5, cujos fatores foram quatro tipos de acondicionamentos de armazenagem (sacarias convencionais, bolsas seladas hermeticamente, silo metálico e espigas) e cinco períodos de tempo (0, 3, 6, 9 e 12 meses). Utilizaram-se dois híbridos de milho (2B688RR, 30K73Hx) cultivados na microrregião de Dois Vizinhos, sudoeste do Paraná. A armazenagem foi realizada em condições ambientais e os parâmetros avaliados foram: teor de água, cinzas, proteínas, lipídios, grãos sem defeitos, peso volumétrico, peso de mil grãos, contagem de Aspergillus sp., Fusarium sp., Penicillium sp., total de bolores e leveduras e ocorrência de aflatoxinas (B1, B2, G1 e G2). Os resultados obtidos em cada experimento, quando atendiam às pressuposições do modelo matemático, eram avaliados pelo emprego da análise de variância e as diferenças entre as médias (p < 0,05) pelo teste de Tukey, a 5% de significância, quando não, pelo teste de Kruskal-Wallis, também a 5% de significância. Além disso, realizou-se a análise dos componentes principais (ACP) para verificar quais das variáveis avaliadas, eram responsáveis pelas maiores variações na qualidade do milho armazenado. Verificou-se, em ambos os armazenamentos, que os grãos de milho acondicionados em bolsas herméticas apresentaram o menor teor de água. Independente do acondicionamento e do híbrido de milho houve aumento do conteúdo de cinzas dos grãos, redução da percentagem de grãos sem defeitos e do peso volumétrico. Constatou-se a incidência de Aspergillus sp., Fusarium sp. e Penicillium sp., respectivamente, em 20,37, 86,11 e 94,44% das amostras de milho do armazenamento safra de inverno; em 83,3, 91,6 e 90,07% das amostras do armazenamento safra de verão, respectivamente. O Aspergillus sp. foi detectado somente aos 3 meses de armazenagem no primeiro experimento, enquanto no segundo desde a colheita. A ocorrência de Fusarium sp. tendeu a diminuir durante a armazenagem e a de Penicillium sp. foi maior no milho acondicionado em espigas. Não houve influência dos tipos de acondicionamentos e do tempo de armazenagem na ocorrência de Aspergillus sp. e de aflatoxinas no milho do armazenamento safra de inverno. Já o milho acondicionado em espigas do armazenamento safra de verão apresentou a menor contagem de Aspergillus sp. e não apresentou aflatoxinas. Os níveis de aflatoxinas (B1+B2+G1+G2) detectados variaram de 2,87 a 14,45 &#956;g kg-1 e de 3,03 a 197,51 &#956;g kg-1, respectivamente no milho dos armazenamentos safra de inverno e safra de verão, além disso, nenhuma das amostras do primeiro armazenamento apresentou contaminação acima de 20 &#956;g kg-1. No segundo armazenamento, 41 amostras apresentaram contaminação. Resultados atribuídos, principalmente, às diferentes condições climáticas dos períodos de cultivo dos híbridos de milho (safra de verão e safra de inverno) e dos experimentos de armazenagem, a disparidade na incidência de Aspergillus sp. e na contaminação por aflatoxinas. Conclui-se, pela ACP, que todas as variáveis aferidas neste trabalho têm importância na qualidade do milho armazenado.Made available in DSpace on 2017-07-10T19:23:52Z (GMT). No. of bitstreams: 1 Adriana Sbardeloto Di _Domenico.pdf: 3233687 bytes, checksum: 1957cd3e9f309d6954a03c673b9b5592 (MD5) Previous issue date: 2014-02-28application/pdfporUniversidade Estadual do Oeste do ParanaPrograma de Pós-Graduação "Stricto Sensu" em Engenharia AgrícolaUNIOESTEBREngenhariaarmazenamentomicotoxinasfungosgrãosZea mays Lpós-colheita e inocuidade dos alimentosstoragemycotoxinsfungigrainsZea mays Lpost-harvest and food safetyCNPQ::CIENCIAS AGRARIAS::ENGENHARIA AGRICOLAQualidade e segurança alimentar do milho em diferentes acondicionamentos de armazenagemFood quality and safety of maize in different types of storageinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/doctoralThesisinfo:eu-repo/semantics/openAccessreponame:Biblioteca Digital de Teses e Dissertações do UNIOESTEinstname:Universidade Estadual do Oeste do Paraná (UNIOESTE)instacron:UNIOESTEORIGINALAdriana Sbardeloto Di _Domenico.pdfapplication/pdf3233687http://tede.unioeste.br:8080/tede/bitstream/tede/2658/1/Adriana+Sbardeloto+Di+_Domenico.pdf1957cd3e9f309d6954a03c673b9b5592MD51tede/26582017-07-11 10:04:12.34oai:tede.unioeste.br:tede/2658Biblioteca Digital de Teses e Dissertaçõeshttp://tede.unioeste.br/PUBhttp://tede.unioeste.br/oai/requestbiblioteca.repositorio@unioeste.bropendoar:2017-07-11T13:04:12Biblioteca Digital de Teses e Dissertações do UNIOESTE - Universidade Estadual do Oeste do Paraná (UNIOESTE)false
dc.title.por.fl_str_mv Qualidade e segurança alimentar do milho em diferentes acondicionamentos de armazenagem
dc.title.alternative.eng.fl_str_mv Food quality and safety of maize in different types of storage
title Qualidade e segurança alimentar do milho em diferentes acondicionamentos de armazenagem
spellingShingle Qualidade e segurança alimentar do milho em diferentes acondicionamentos de armazenagem
Domenico, Adriana Sbardelotto Di
armazenamento
micotoxinas
fungos
grãos
Zea mays L
pós-colheita e inocuidade dos alimentos
storage
mycotoxins
fungi
grains
Zea mays L
post-harvest and food safety
CNPQ::CIENCIAS AGRARIAS::ENGENHARIA AGRICOLA
title_short Qualidade e segurança alimentar do milho em diferentes acondicionamentos de armazenagem
title_full Qualidade e segurança alimentar do milho em diferentes acondicionamentos de armazenagem
title_fullStr Qualidade e segurança alimentar do milho em diferentes acondicionamentos de armazenagem
title_full_unstemmed Qualidade e segurança alimentar do milho em diferentes acondicionamentos de armazenagem
title_sort Qualidade e segurança alimentar do milho em diferentes acondicionamentos de armazenagem
author Domenico, Adriana Sbardelotto Di
author_facet Domenico, Adriana Sbardelotto Di
author_role author
dc.contributor.advisor1.fl_str_mv Christ, Divair
dc.contributor.advisor1ID.fl_str_mv CPF:66206863972
dc.contributor.advisor1Lattes.fl_str_mv http://lattes.cnpq.br/6200553304840204
dc.contributor.referee1.fl_str_mv Coelho, Silvia Renata Machado
dc.contributor.referee1ID.fl_str_mv CPF:88213943600
dc.contributor.referee1Lattes.fl_str_mv http://lattes.cnpq.br/3554106124561773
dc.contributor.referee2.fl_str_mv Frata, Marcela Tostes
dc.contributor.referee2ID.fl_str_mv CPF:00335688985
dc.contributor.referee2Lattes.fl_str_mv http://lattes.cnpq.br/8243438180476961
dc.contributor.referee3.fl_str_mv Sousa, Fernando Carlos de
dc.contributor.referee3ID.fl_str_mv CPF:02781191906
dc.contributor.referee3Lattes.fl_str_mv http://lattes.cnpq.br/1004312837873706
dc.contributor.referee4.fl_str_mv Busso, Cleverson
dc.contributor.referee4ID.fl_str_mv CPF:02755772956
dc.contributor.referee4Lattes.fl_str_mv http://lattes.cnpq.br/5986131313813011
dc.contributor.authorID.fl_str_mv CPF:0376609690
dc.contributor.authorLattes.fl_str_mv http://lattes.cnpq.br/7513246316553733
dc.contributor.author.fl_str_mv Domenico, Adriana Sbardelotto Di
contributor_str_mv Christ, Divair
Coelho, Silvia Renata Machado
Frata, Marcela Tostes
Sousa, Fernando Carlos de
Busso, Cleverson
dc.subject.por.fl_str_mv armazenamento
micotoxinas
fungos
grãos
Zea mays L
pós-colheita e inocuidade dos alimentos
topic armazenamento
micotoxinas
fungos
grãos
Zea mays L
pós-colheita e inocuidade dos alimentos
storage
mycotoxins
fungi
grains
Zea mays L
post-harvest and food safety
CNPQ::CIENCIAS AGRARIAS::ENGENHARIA AGRICOLA
dc.subject.eng.fl_str_mv storage
mycotoxins
fungi
grains
Zea mays L
post-harvest and food safety
dc.subject.cnpq.fl_str_mv CNPQ::CIENCIAS AGRARIAS::ENGENHARIA AGRICOLA
description Brazil is one of the largest producers of grains, and maize is the second most produced grain in the country. As this cereal is directly related to human and animal food basis, many matters appear on the maintenance of their post-harvest quality, especially during storage, due to the increased attention to food safety. Despite this importance, maize is often stored on farms in inappropriate conditions, exposed to quantitative and qualitative losses, the development of toxigenic fungi and aflatoxin contamination, a substance capable of causing severe damage to human and animal health, as well as many agribusiness losses. The objective of this research was evaluating storage alternatives viable to farms through two storage experiments (2012 winter crop 2012/2013 summer harvests), for assessment of the quality of stored maize in different packings for 12 months. The experimental design was a 4 x 5 factorial, whose factors were four types of packaging storage (conventional sacks, bags hermetically sealed, metallic silo and cobs) and five time periods (0, 3, 6, 9 and 12 months). Two maize hybrids (2B688RR, 30K73Hx) cultivated in the region of the town of Dois Vizinhos were used, in the southwest of Paraná. The storage was conducted in ambient conditions and the following parameters were evaluated: water content, ash, protein, fat, grains without defects, volumetric weight, one-thousand grains weight, counting of Aspergillus sp, Fusarium sp, Penicillium sp, total of molds and yeasts and occurrence of aflatoxins (B1, B2, G1 and G2). The results obtained in each experiment, when attended the presuppositions of the mathematical model, were evaluated by use of analysis of variance and differences between means (p<0.05) by Tukey test at 5% significance level, and when not attended them, by Kruskal-Wallis also at 5% significance level. Furthermore, the Principal Component Analysis (PCA) was done to determine which of the variables evaluated was responsible for the largest variations in the quality of stored maize. It was found in both storages that maize kernels placed in sealed bags presented the lowest water content. Also that regardless of packaging and hybrid maize, there were an increase in ash content of grains and the decrease of the percentage of grains without defects and volumetric weight. It was detected the incidence of Aspergillus sp. Fusarium sp. and Penicillium sp., respectively 20.37, 86.11 and 94.44% of the samples of maize season winter storage, and in 83.3, 91.6 and 90.07% of the samples from the summer season storage. Aspergillus sp. was only detected after 3 months of storage in the first experiment, while in the second since harvesting. The occurrence of Fusarium sp. tended to decrease during storage, and Penicillium sp. was higher in corn conditioning in cobs. There was no influence of types of packaging and storage time on the occurrence of Aspergillus sp. and aflatoxins in maize season winter storage. However, the conditioning in maize cobs of season storage summer had the lowest count of Aspergillus sp. and did not present aflatoxin. Aflatoxins (B1+B2+G1+G2) detected ranged from 2.77 to 14.45 &#956;g kg-1 and from 3.03 to 197.51 &#956;g kg-1 respectively in the maize storage winter and summer. Besides this, none of the samples of the first storage showed contamination higher than 20 &#956;g kg-1, while in the second 41 samples presented higher values. The results are due to the different climatic conditions of the periods of cultivation of hybrid maize (summer and winter seasons) and storage experiments, the disparity in the incidence of Aspergillus sp. and aflatoxin contamination. The conclusion is that by ACP all variables assessed in this study are important for the quality of stored maize.
publishDate 2014
dc.date.issued.fl_str_mv 2014-02-28
dc.date.available.fl_str_mv 2015-08-25
dc.date.accessioned.fl_str_mv 2017-07-10T19:23:52Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
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dc.identifier.citation.fl_str_mv DOMENICO, Adriana Sbardelotto Di. Food quality and safety of maize in different types of storage. 2014. 156 f. Tese (Doutorado em Engenharia) - Universidade Estadual do Oeste do Parana, Cascavel, 2014.
dc.identifier.uri.fl_str_mv http://tede.unioeste.br:8080/tede/handle/tede/2658
identifier_str_mv DOMENICO, Adriana Sbardelotto Di. Food quality and safety of maize in different types of storage. 2014. 156 f. Tese (Doutorado em Engenharia) - Universidade Estadual do Oeste do Parana, Cascavel, 2014.
url http://tede.unioeste.br:8080/tede/handle/tede/2658
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dc.publisher.none.fl_str_mv Universidade Estadual do Oeste do Parana
dc.publisher.program.fl_str_mv Programa de Pós-Graduação "Stricto Sensu" em Engenharia Agrícola
dc.publisher.initials.fl_str_mv UNIOESTE
dc.publisher.country.fl_str_mv BR
dc.publisher.department.fl_str_mv Engenharia
publisher.none.fl_str_mv Universidade Estadual do Oeste do Parana
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