Baccharis dracunculifolia D.C: diversidade genética e química de populações

• Ano de defesa: 2011
• Autor(a) principal: Rigotti, Marcelo [UNESP]
• Tipo de documento: Tese
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Estadual Paulista (Unesp)
• Palavras-chave em Português: Diversidade genética; Plantas aromaticas; Marcadores moleculares; Genetica de populações; Populations genetics
• Link de acesso: http://hdl.handle.net/11449/133997; http://www.athena.biblioteca.unesp.br/exlibris/bd/cathedra/03-02-2016/000858227.pdf

The species Baccharis dracunculifolia, popularly known as rosemary-of-field, is widely used in folk medicine. The form of infusion of its leaves is used for liver problems, stomach disorders and as anti-inflammatory. Studies have reported the use of medical and religious rosemary-of-field sold in markets and fairs in Rio de Janeiro, as well as the use of leaves for wounds and the use of branches as decoction as antipyretic. This study aimed to characterize the chemical composition of essential oils and genetic diversity of native species B. dracunculifolia DC (Asteraceae) in the Cerrado biome. Were collected, on average, 30 individuals from four natural populations, one in Botucatu, in the state of São Paulo, a city of Delfinópolis in the state of Minas Gerais and two in Dourados and Itahum, state of Mato Grosso do Sul. The chemical composition of essential oil extracted by hydrodistillation, was analyzed by gas chromatography coupled with mass spectrometer. The majority of accessions collected substances were trans-nerolidol, cis-β-guaieno, germacrene-D, trans-caryophyllene, limonene, α-pinene, β-myrcene, delta-cadinene, β-pinene and spathulenol. These components amounted to an overall average of 80.97% of the chemical composition of essential oils in four locations. According to the analysis results of the substance trans-nerolidol was the most outstanding among which the present compounds in the essential oil of the species B. dracunculifolia in all populations. However, the accessions were collected in Itahum spathulenol as major components followed by the trans-nerolidol. The study of genetic diversity was performed by analyzing the DNA polymorphism using microsatellite markers. Five microsatellite loci were tested for the species revealed 48 alleles for the four populations ...