Impacto da exposição á misturas químicas de desreguladores endócrinos desde a gestação até a idade adulta no coração de ratos Wistar
Ano de defesa: | 2023 |
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Autor(a) principal: | |
Orientador(a): | |
Banca de defesa: | , |
Tipo de documento: | Dissertação |
Tipo de acesso: | Acesso aberto |
Idioma: | por |
Instituição de defesa: |
Universidade do Oeste Paulista
|
Programa de Pós-Graduação: |
Mestrado em Ciências da Saúde
|
Departamento: |
Mestrado em Ciências da Saúde
|
País: |
Brasil
|
Palavras-chave em Português: | |
Palavras-chave em Inglês: | |
Área do conhecimento CNPq: | |
Link de acesso: | http://bdtd.unoeste.br:8080/jspui/handle/jspui/1539 |
Resumo: | Endocrine disruptors (ED) are substances that alter hormonal biosynthesis and promote toxic effects, causing cardiovascular diseases. However, the long-term effects of association of several ND on cardiac remodeling have been elucidated. The aim of this study was to evaluate the chronic effects of a DE mixture on cardiac remodeling in aspects related to structure, function and oxidative stress. Pregnant females of the Sprague-Dawley strain were divided into 2 experimental groups: CT Group (vehicle: corn oil, by gavage) and DE Mix Group: received 32.11mg/kg/day of a mixture consisting of twelve DE (phthalates, pesticides, u.v. filters, bisphenol A, butylparaben) diluted in corn oil (2ml/kg), by gavage. Pregnant or lactating rats received the treatment from gestational day 7 (DG7) until postnatal day 21 (DPN21). After weaning, at DPN22, the male offspring of the F1 generation continued to receive the DE mixture until they were 220 days old, after which they were euthanized. After this period, echocardiographic and histopathological analysis and analysis of oxidative stress were performed. There were no changes in the systolic (endocardial shortening: CT- 44.44 ± 3.12 % vs. DE Mix- 44.70 ± 3.32) and diastolic function of the left ventricle (E/A wave: CT- 3.37 ± 1.15 vs. DE Mix- 3.68 ± 1.20). It was possible to observe a significant decrease in the area of cardiomyocytes (CT- 347 ± 91.62 µm2 vs. DE Mix- 202 ± 72.70 µm2, p < 0.01) and there were no changes in the fractal dimension. Lipid peroxidation levels were reduced in the DE Mix Group (CT- 5.73 ± 0.36 vs. DE Mix- 5.17 ± 0.60 nmol/mg of protein, p = 0.008). The enzyme superoxide dismutase (SOD, CT- 10.18 ± 9.25 vs. DE Mix- 4.93 ± 1.48 U/ mg of protein, p= 0.10) and reduced Glutathione (GSH, CT- 28 .47 ± 15.17 vs. DE Mix- 39.66 ± 43.85 µM/mg protein, p= 0.53) did not change. There was a decrease in Glutathione -S- Transferase (GST, CT- 138.3 ± 28.44 vs. DE Mix- 113.8 ± 48.44, mM/mg of protein, p= 0.01). The chronic mixture of ED, since pregnancy, had a negative impact on the cardiac microenvironment, promoting cardiac atrophy and changes in oxidative stress markers, without promoting functional changes. |
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Pacagnelli, Francis Lopeshttps://orcid.org/0000-0003-3712-5854http://lattes.cnpq.br/2275399100240086Fernandes , Glaura Scantamburlo Alveshttps://orcid.org/0000-0002-6043-1131http://lattes.cnpq.br/0503733087170121Santarém , Cecília Laposyhttps://orcid.org/0000-0002-4775-6142http://lattes.cnpq.br/6243360005165063https://orcid.org/0000-0002-4523-9256http://lattes.cnpq.br/1920402734936471Rodrigues, Alessandra Gomes Duarte2023-12-01T18:52:49Z2023-03-06RODRIGUES, Alessandra Gomes Duarte. Impacto da exposição á misturas químicas de desreguladores endócrinos desde a gestação até a idade adulta no coração de ratos Wistar. 2023. Dissertação (Mestrado em Ciências da Saúde) - Universidade do Oeste Paulista, Presidente Prudente, SP, 2023.http://bdtd.unoeste.br:8080/jspui/handle/jspui/1539Endocrine disruptors (ED) are substances that alter hormonal biosynthesis and promote toxic effects, causing cardiovascular diseases. However, the long-term effects of association of several ND on cardiac remodeling have been elucidated. The aim of this study was to evaluate the chronic effects of a DE mixture on cardiac remodeling in aspects related to structure, function and oxidative stress. Pregnant females of the Sprague-Dawley strain were divided into 2 experimental groups: CT Group (vehicle: corn oil, by gavage) and DE Mix Group: received 32.11mg/kg/day of a mixture consisting of twelve DE (phthalates, pesticides, u.v. filters, bisphenol A, butylparaben) diluted in corn oil (2ml/kg), by gavage. Pregnant or lactating rats received the treatment from gestational day 7 (DG7) until postnatal day 21 (DPN21). After weaning, at DPN22, the male offspring of the F1 generation continued to receive the DE mixture until they were 220 days old, after which they were euthanized. After this period, echocardiographic and histopathological analysis and analysis of oxidative stress were performed. There were no changes in the systolic (endocardial shortening: CT- 44.44 ± 3.12 % vs. DE Mix- 44.70 ± 3.32) and diastolic function of the left ventricle (E/A wave: CT- 3.37 ± 1.15 vs. DE Mix- 3.68 ± 1.20). It was possible to observe a significant decrease in the area of cardiomyocytes (CT- 347 ± 91.62 µm2 vs. DE Mix- 202 ± 72.70 µm2, p < 0.01) and there were no changes in the fractal dimension. Lipid peroxidation levels were reduced in the DE Mix Group (CT- 5.73 ± 0.36 vs. DE Mix- 5.17 ± 0.60 nmol/mg of protein, p = 0.008). The enzyme superoxide dismutase (SOD, CT- 10.18 ± 9.25 vs. DE Mix- 4.93 ± 1.48 U/ mg of protein, p= 0.10) and reduced Glutathione (GSH, CT- 28 .47 ± 15.17 vs. DE Mix- 39.66 ± 43.85 µM/mg protein, p= 0.53) did not change. There was a decrease in Glutathione -S- Transferase (GST, CT- 138.3 ± 28.44 vs. DE Mix- 113.8 ± 48.44, mM/mg of protein, p= 0.01). The chronic mixture of ED, since pregnancy, had a negative impact on the cardiac microenvironment, promoting cardiac atrophy and changes in oxidative stress markers, without promoting functional changes.Os desreguladores endócrinos (DE) são substâncias que alteram a biossíntese hormonal e promovem efeitos tóxicos ocasionando doenças cardiovasculares. Entretanto, os efeitos da associação de vários DE a longo prazo na remodelação cardíaca não foram elucidados. O objetivo desse estudo foi avaliar os efeitos crônicos de uma mistura de DE na remodelação cardíaca de ratos nos aspectos relacionados a estrutura, função e nos marcadores de estresse oxidativo. Fêmeas prenhes da linhagem Sprague-Dawley foram divididas em 2 grupos experimentais: Grupo CT (veículo: óleo de milho, por gavagem) e Grupo DE Mix: receberam 32,11mg/kg/dia da mistura constituída de doze DE (ftalatos, pesticidas, filtros u.v., bisfenol A, butilparabeno) diluídos em óleo de milho (2ml/kg), por gavagem. As ratas prenhes ou lactentes receberam o tratamento do dia gestacional 7 (DG7) até o dia pós-natal 21 (DPN21). Após o desmame, no DPN22, os filhotes machos da geração F1 continuaram a receber a mistura de DE até os 220 dias de idade, e após foram eutanasiados. Após esse período foram realizadas a análise ecocardiográfica, histopatológica e análise do estresse oxidativo. Não houve alterações da função sistólica (encurtamento do endocárdio: CT- 44,44 ± 3,12 % vs. DE Mix- 44,70 ± 3,32%) e diastólica do ventrículo esquerdo (Onda E/A: CT- 3,37 ± 1,15 vs. DE Mix- 3,68 ± 1,20). Foi possível observar uma diminuição significativa na área dos cardiomiócitos (CT- 347 ± 91,62 µm2 vs. DE Mix- 202 ± 72,70 µm2, p < 0,01) e não houve alterações da dimensão fractal. Os níveis de peroxidação lipídica estavam reduzidos no Grupo DE Mix (CT- 5,73 ± 0,36 vs. DE Mix- 5,17 ± 0,60 nmol/mg de proteína, p = 0,008). As enzimas superóxido dismutase (SOD, CT- 10,18 ± 9,25 vs. DE Mix- 4,93 ± 1,48 U/ mg de proteína, p= 0,10) e a Glutationa reduzida (GSH, CT- 28,47 ± 15,17 vs. DE Mix- 39,66 ± 43,85 µM/ mg de proteína, p= 0,53) não se alteraram. Houve diminuição da enzima Glutationa -S- Transferase (GST, CT- 138,3 ± 28,44 vs. DE Mix- 113,8 ± 48,44, mM/ mg de proteína, p= 0,01). A mistura de DE de forma crônica, desde a gestação, teve impacto negativo no microambiente cardíaco, promovendo atrofia cardíaca e alterações nos marcadores do estresse oxidativo, sem promover modificações funcionais.Submitted by Jakeline Ortega (jakortega@unoeste.br) on 2023-12-01T18:52:49Z No. of bitstreams: 1 Alessandra Gomes Duarte Rodrigues.pdf: 1317448 bytes, checksum: 4b92dc7aad20295b82fe5b08b0fdc52d (MD5)Made available in DSpace on 2023-12-01T18:52:49Z (GMT). No. of bitstreams: 1 Alessandra Gomes Duarte Rodrigues.pdf: 1317448 bytes, checksum: 4b92dc7aad20295b82fe5b08b0fdc52d (MD5) Previous issue date: 2023-03-06application/pdfhttp://bdtd.unoeste.br:8080/jspui/retrieve/5394/Alessandra%20Gomes%20Duarte%20Rodrigues.pdf.jpgporUniversidade do Oeste PaulistaMestrado em Ciências da SaúdeUNOESTEBrasilMestrado em Ciências da SaúdeAtrofia cardíacaDesregulador endócrinoEstresse oxidativoSistema cardiovascularFractalCardiac atrophyEndocrine disruptorOxidative stressCardiovascular systemCIENCIAS DA SAUDE::MEDICINAImpacto da exposição á misturas químicas de desreguladores endócrinos desde a gestação até a idade adulta no coração de ratos WistarThe impact of a mixture endocrine-disrupting chemical exposure during gestational and adult period in the heart in wistar ratsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesis45484740625200741685005006004548474062520074168-969369452308786627info:eu-repo/semantics/openAccessreponame:Biblioteca Digital de Teses e Dissertações da UNOESTEinstname:Universidade do Oeste Paulista (UNOESTE)instacron:UNOESTETHUMBNAILAlessandra Gomes Duarte Rodrigues.pdf.jpgAlessandra Gomes Duarte Rodrigues.pdf.jpgimage/jpeg2179http://bdtd.unoeste.br:8080/tede/bitstream/jspui/1539/4/Alessandra+Gomes+Duarte+Rodrigues.pdf.jpgfdfdb7c62faf6021f2cb1443d679c269MD54TEXTAlessandra Gomes Duarte Rodrigues.pdf.txtAlessandra Gomes Duarte Rodrigues.pdf.txttext/plain72779http://bdtd.unoeste.br:8080/tede/bitstream/jspui/1539/3/Alessandra+Gomes+Duarte+Rodrigues.pdf.txt4dfe1fd47ab1cf7d56fdd9392c847f73MD53ORIGINALAlessandra Gomes Duarte Rodrigues.pdfAlessandra Gomes Duarte Rodrigues.pdfapplication/pdf1317448http://bdtd.unoeste.br:8080/tede/bitstream/jspui/1539/2/Alessandra+Gomes+Duarte+Rodrigues.pdf4b92dc7aad20295b82fe5b08b0fdc52dMD52LICENSElicense.txtlicense.txttext/plain; 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dc.title.por.fl_str_mv |
Impacto da exposição á misturas químicas de desreguladores endócrinos desde a gestação até a idade adulta no coração de ratos Wistar |
dc.title.alternative.eng.fl_str_mv |
The impact of a mixture endocrine-disrupting chemical exposure during gestational and adult period in the heart in wistar rats |
title |
Impacto da exposição á misturas químicas de desreguladores endócrinos desde a gestação até a idade adulta no coração de ratos Wistar |
spellingShingle |
Impacto da exposição á misturas químicas de desreguladores endócrinos desde a gestação até a idade adulta no coração de ratos Wistar Rodrigues, Alessandra Gomes Duarte Atrofia cardíaca Desregulador endócrino Estresse oxidativo Sistema cardiovascular Fractal Cardiac atrophy Endocrine disruptor Oxidative stress Cardiovascular system CIENCIAS DA SAUDE::MEDICINA |
title_short |
Impacto da exposição á misturas químicas de desreguladores endócrinos desde a gestação até a idade adulta no coração de ratos Wistar |
title_full |
Impacto da exposição á misturas químicas de desreguladores endócrinos desde a gestação até a idade adulta no coração de ratos Wistar |
title_fullStr |
Impacto da exposição á misturas químicas de desreguladores endócrinos desde a gestação até a idade adulta no coração de ratos Wistar |
title_full_unstemmed |
Impacto da exposição á misturas químicas de desreguladores endócrinos desde a gestação até a idade adulta no coração de ratos Wistar |
title_sort |
Impacto da exposição á misturas químicas de desreguladores endócrinos desde a gestação até a idade adulta no coração de ratos Wistar |
author |
Rodrigues, Alessandra Gomes Duarte |
author_facet |
Rodrigues, Alessandra Gomes Duarte |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Pacagnelli, Francis Lopes |
dc.contributor.advisor1ID.fl_str_mv |
https://orcid.org/0000-0003-3712-5854 |
dc.contributor.advisor1Lattes.fl_str_mv |
http://lattes.cnpq.br/2275399100240086 |
dc.contributor.referee1.fl_str_mv |
Fernandes , Glaura Scantamburlo Alves |
dc.contributor.referee1ID.fl_str_mv |
https://orcid.org/0000-0002-6043-1131 |
dc.contributor.referee1Lattes.fl_str_mv |
http://lattes.cnpq.br/0503733087170121 |
dc.contributor.referee2.fl_str_mv |
Santarém , Cecília Laposy |
dc.contributor.referee2ID.fl_str_mv |
https://orcid.org/0000-0002-4775-6142 |
dc.contributor.referee2Lattes.fl_str_mv |
http://lattes.cnpq.br/6243360005165063 |
dc.contributor.authorID.fl_str_mv |
https://orcid.org/0000-0002-4523-9256 |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/1920402734936471 |
dc.contributor.author.fl_str_mv |
Rodrigues, Alessandra Gomes Duarte |
contributor_str_mv |
Pacagnelli, Francis Lopes Fernandes , Glaura Scantamburlo Alves Santarém , Cecília Laposy |
dc.subject.por.fl_str_mv |
Atrofia cardíaca Desregulador endócrino Estresse oxidativo Sistema cardiovascular Fractal |
topic |
Atrofia cardíaca Desregulador endócrino Estresse oxidativo Sistema cardiovascular Fractal Cardiac atrophy Endocrine disruptor Oxidative stress Cardiovascular system CIENCIAS DA SAUDE::MEDICINA |
dc.subject.eng.fl_str_mv |
Cardiac atrophy Endocrine disruptor Oxidative stress Cardiovascular system |
dc.subject.cnpq.fl_str_mv |
CIENCIAS DA SAUDE::MEDICINA |
description |
Endocrine disruptors (ED) are substances that alter hormonal biosynthesis and promote toxic effects, causing cardiovascular diseases. However, the long-term effects of association of several ND on cardiac remodeling have been elucidated. The aim of this study was to evaluate the chronic effects of a DE mixture on cardiac remodeling in aspects related to structure, function and oxidative stress. Pregnant females of the Sprague-Dawley strain were divided into 2 experimental groups: CT Group (vehicle: corn oil, by gavage) and DE Mix Group: received 32.11mg/kg/day of a mixture consisting of twelve DE (phthalates, pesticides, u.v. filters, bisphenol A, butylparaben) diluted in corn oil (2ml/kg), by gavage. Pregnant or lactating rats received the treatment from gestational day 7 (DG7) until postnatal day 21 (DPN21). After weaning, at DPN22, the male offspring of the F1 generation continued to receive the DE mixture until they were 220 days old, after which they were euthanized. After this period, echocardiographic and histopathological analysis and analysis of oxidative stress were performed. There were no changes in the systolic (endocardial shortening: CT- 44.44 ± 3.12 % vs. DE Mix- 44.70 ± 3.32) and diastolic function of the left ventricle (E/A wave: CT- 3.37 ± 1.15 vs. DE Mix- 3.68 ± 1.20). It was possible to observe a significant decrease in the area of cardiomyocytes (CT- 347 ± 91.62 µm2 vs. DE Mix- 202 ± 72.70 µm2, p < 0.01) and there were no changes in the fractal dimension. Lipid peroxidation levels were reduced in the DE Mix Group (CT- 5.73 ± 0.36 vs. DE Mix- 5.17 ± 0.60 nmol/mg of protein, p = 0.008). The enzyme superoxide dismutase (SOD, CT- 10.18 ± 9.25 vs. DE Mix- 4.93 ± 1.48 U/ mg of protein, p= 0.10) and reduced Glutathione (GSH, CT- 28 .47 ± 15.17 vs. DE Mix- 39.66 ± 43.85 µM/mg protein, p= 0.53) did not change. There was a decrease in Glutathione -S- Transferase (GST, CT- 138.3 ± 28.44 vs. DE Mix- 113.8 ± 48.44, mM/mg of protein, p= 0.01). The chronic mixture of ED, since pregnancy, had a negative impact on the cardiac microenvironment, promoting cardiac atrophy and changes in oxidative stress markers, without promoting functional changes. |
publishDate |
2023 |
dc.date.accessioned.fl_str_mv |
2023-12-01T18:52:49Z |
dc.date.issued.fl_str_mv |
2023-03-06 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
RODRIGUES, Alessandra Gomes Duarte. Impacto da exposição á misturas químicas de desreguladores endócrinos desde a gestação até a idade adulta no coração de ratos Wistar. 2023. Dissertação (Mestrado em Ciências da Saúde) - Universidade do Oeste Paulista, Presidente Prudente, SP, 2023. |
dc.identifier.uri.fl_str_mv |
http://bdtd.unoeste.br:8080/jspui/handle/jspui/1539 |
identifier_str_mv |
RODRIGUES, Alessandra Gomes Duarte. Impacto da exposição á misturas químicas de desreguladores endócrinos desde a gestação até a idade adulta no coração de ratos Wistar. 2023. Dissertação (Mestrado em Ciências da Saúde) - Universidade do Oeste Paulista, Presidente Prudente, SP, 2023. |
url |
http://bdtd.unoeste.br:8080/jspui/handle/jspui/1539 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.program.fl_str_mv |
4548474062520074168 |
dc.relation.confidence.fl_str_mv |
500 500 600 |
dc.relation.department.fl_str_mv |
4548474062520074168 |
dc.relation.cnpq.fl_str_mv |
-969369452308786627 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade do Oeste Paulista |
dc.publisher.program.fl_str_mv |
Mestrado em Ciências da Saúde |
dc.publisher.initials.fl_str_mv |
UNOESTE |
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Brasil |
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Mestrado em Ciências da Saúde |
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Universidade do Oeste Paulista |
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UNOESTE |
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UNOESTE |
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Biblioteca Digital de Teses e Dissertações da UNOESTE |
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Biblioteca Digital de Teses e Dissertações da UNOESTE - Universidade do Oeste Paulista (UNOESTE) |
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bdtd@unoeste.br |
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