Efeito de nanocarreadores de drogas antifúngicas sobre espécies de Candida em biofilmes microcosmos
Ano de defesa: | 2021 |
---|---|
Autor(a) principal: | |
Orientador(a): | |
Banca de defesa: | , |
Tipo de documento: | Dissertação |
Tipo de acesso: | Acesso aberto |
Idioma: | por |
Instituição de defesa: |
Universidade do Oeste Paulista
|
Programa de Pós-Graduação: |
Mestrado em Odontologia
|
Departamento: |
Mestrado em Odontologia
|
País: |
Brasil
|
Palavras-chave em Português: | |
Palavras-chave em Inglês: | |
Área do conhecimento CNPq: | |
Link de acesso: | http://bdtd.unoeste.br:8080/jspui/handle/jspui/1393 |
Resumo: | Resistance of Candida species to conventional therapies has motivated the development of antifungal nanocarriers based on iron oxide nanoparticles (IONPs) coated with chitosan (CS). This study evaluated the effects of IONPs-CS as carriers of miconazole (MCZ) or fluconazole (FLZ) on microcosm biofilms. Pooled saliva from two healthy volunteers supplemented with Candida albicans and Candida glabrata was the inoculum for biofilm formation. Biofilms were formed for 96 h on coverslips using the Amsterdam Active Attachment model, followed by 24 h treatment with nanocarriers containing different concentrations of each antifungal (78 and 156 µg/mL). MCZ or FLZ (156 µg/mL), and untreated biofilms were considered as controls. Anti-biofilm effects were evaluated by enumeration of colony-forming units (CFUs), composition of the extracellular matrix, lactic acid production, and structure and live/dead biofilm cells (confocal laser scanning microscopy - CLSM). Data were analyzed by 1-way ANOVA and Fisher LSD’s test (α = 0.05). IONPs-CS carrying MCZ or FLZ were the most effective treatments in reducing CFUs compared to either antifungal agent alone for C. albicans and MCZ for C. glabrata. Significant reductions in mutans streptococci and Lactobacillus spp. were shown, though mainly for the MCZ nanocarrier. Antifungals and their nanocarriers also showed significantly higher proportions of dead cells compared to untreated biofilm by CLSM (p < 0.001), and promoted significant reductions in lactic acid, while simultaneously showing increases in some components of the extracellular matrix. These findings reinforce the use of nanocarriers as effective alternatives to fight oral fungal infections. |
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Monteiro, Douglas Robertohttp://lattes.cnpq.br/2795875575479712Batista, Victor Eduardo de Souzahttp://lattes.cnpq.br/3172143203186731Pessan, Juliano Pelimhttp://lattes.cnpq.br/2591135229049859http://lattes.cnpq.br/7890610182758459CALDEIRÃO, Anne Caroline Morais2022-04-07T18:12:28Z2021-03-19CALDEIRÃO, Anne Caroline Morais. Efeito de nanocarreadores de drogas antifúngicas sobre espécies de Candida em biofilmes microcosmos. 2021. Dissertação (Mestrado em Odontologia) - Universidade do Oeste Paulista, Presidente Prudente, SP, 2021.http://bdtd.unoeste.br:8080/jspui/handle/jspui/1393Resistance of Candida species to conventional therapies has motivated the development of antifungal nanocarriers based on iron oxide nanoparticles (IONPs) coated with chitosan (CS). This study evaluated the effects of IONPs-CS as carriers of miconazole (MCZ) or fluconazole (FLZ) on microcosm biofilms. Pooled saliva from two healthy volunteers supplemented with Candida albicans and Candida glabrata was the inoculum for biofilm formation. Biofilms were formed for 96 h on coverslips using the Amsterdam Active Attachment model, followed by 24 h treatment with nanocarriers containing different concentrations of each antifungal (78 and 156 µg/mL). MCZ or FLZ (156 µg/mL), and untreated biofilms were considered as controls. Anti-biofilm effects were evaluated by enumeration of colony-forming units (CFUs), composition of the extracellular matrix, lactic acid production, and structure and live/dead biofilm cells (confocal laser scanning microscopy - CLSM). Data were analyzed by 1-way ANOVA and Fisher LSD’s test (α = 0.05). IONPs-CS carrying MCZ or FLZ were the most effective treatments in reducing CFUs compared to either antifungal agent alone for C. albicans and MCZ for C. glabrata. Significant reductions in mutans streptococci and Lactobacillus spp. were shown, though mainly for the MCZ nanocarrier. Antifungals and their nanocarriers also showed significantly higher proportions of dead cells compared to untreated biofilm by CLSM (p < 0.001), and promoted significant reductions in lactic acid, while simultaneously showing increases in some components of the extracellular matrix. These findings reinforce the use of nanocarriers as effective alternatives to fight oral fungal infections.A resistência das espécies de Candida às terapias convencionais tem motivado o desenvolvimento de nanocarreadores antifúngicos baseados em nanopartículas de óxido de ferro (NPsOF) revestidas com quitosana (QTS). Este estudo avaliou os efeitos de NPsOF-QTS como carreadores de miconazol (MCZ) ou fluconazol (FLZ) sobre biofilmes microcosmos salivares. Pool de saliva de dois voluntários saudáveis suplementada com Candida albicans e Candida glabrata foi o inóculo para a formação de biofilmes. Os biofilmes foram formados por 96 horas sobre discos de vidro no Amsterdam Active Attachment Model e tratados por 24 horas com os nanocarreadores contendo diferentes concentrações de cada antifúngico (78 e 156 µg/mL). MCZ ou FLZ (156 µg/mL) e biofilmes não tratados foram considerados como controles. Os efeitos antibiofilme foram avaliados pela enumeração das unidades formadoras de colônias (UFCs), composição da matriz extracelular, produção de ácido lático, além da análise da estrutura e das células vivas/mortas do biofilme por microscopia confocal de varredura a laser (MCVL). Os dados foram analisados por ANOVA a um critério e teste de Fisher LSD (α = 0,05). NPsOF-QTS carreando MCZ ou FLZ foram os tratamentos mais eficazes na redução de UFCs em comparação com qualquer agente antifúngico sozinho para C. albicans e com MCZ para C. glabrata. Reduções significativas em estreptococos do grupo mutans e Lactobacillus spp. foram encontradas, principalmente para o nanocarreador de MCZ. Os antifúngicos e seus nanocarreadores também mostraram proporções significativamente maiores de células mortas em comparação ao biofilme não tratado por MCVL (p < 0,001), promoveram reduções significativas na produção de ácido lático e aumentaram significativamente alguns componentes da matriz extracelular. Esses resultados reforçam o uso de nanocarreadores como alternativas eficazes para combater infecções fúngicas orais.Submitted by Jakeline Ortega (jakortega@unoeste.br) on 2022-04-07T18:12:28Z No. of bitstreams: 2 Anne Caroline Morais Caldeirão.pdf: 4707061 bytes, checksum: 512a846bb4873367ee06b663811b9c7e (MD5) license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5)Made available in DSpace on 2022-04-07T18:12:28Z (GMT). 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dc.title.por.fl_str_mv |
Efeito de nanocarreadores de drogas antifúngicas sobre espécies de Candida em biofilmes microcosmos |
dc.title.alternative.eng.fl_str_mv |
Effect of nanocarriers of antifungal drugs on Candida species in microcosm biofilms |
title |
Efeito de nanocarreadores de drogas antifúngicas sobre espécies de Candida em biofilmes microcosmos |
spellingShingle |
Efeito de nanocarreadores de drogas antifúngicas sobre espécies de Candida em biofilmes microcosmos CALDEIRÃO, Anne Caroline Morais Antifúngicos Biofilmes Candida Nanocarreadores Nanopartículas de óxido de ferro Antifungals Biofilms Candida Nanocarriers Iron oxide nanoparticles CIENCIAS DA SAUDE::ODONTOLOGIA |
title_short |
Efeito de nanocarreadores de drogas antifúngicas sobre espécies de Candida em biofilmes microcosmos |
title_full |
Efeito de nanocarreadores de drogas antifúngicas sobre espécies de Candida em biofilmes microcosmos |
title_fullStr |
Efeito de nanocarreadores de drogas antifúngicas sobre espécies de Candida em biofilmes microcosmos |
title_full_unstemmed |
Efeito de nanocarreadores de drogas antifúngicas sobre espécies de Candida em biofilmes microcosmos |
title_sort |
Efeito de nanocarreadores de drogas antifúngicas sobre espécies de Candida em biofilmes microcosmos |
author |
CALDEIRÃO, Anne Caroline Morais |
author_facet |
CALDEIRÃO, Anne Caroline Morais |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Monteiro, Douglas Roberto |
dc.contributor.advisor1Lattes.fl_str_mv |
http://lattes.cnpq.br/2795875575479712 |
dc.contributor.referee1.fl_str_mv |
Batista, Victor Eduardo de Souza |
dc.contributor.referee1Lattes.fl_str_mv |
http://lattes.cnpq.br/3172143203186731 |
dc.contributor.referee2.fl_str_mv |
Pessan, Juliano Pelim |
dc.contributor.referee2Lattes.fl_str_mv |
http://lattes.cnpq.br/2591135229049859 |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/7890610182758459 |
dc.contributor.author.fl_str_mv |
CALDEIRÃO, Anne Caroline Morais |
contributor_str_mv |
Monteiro, Douglas Roberto Batista, Victor Eduardo de Souza Pessan, Juliano Pelim |
dc.subject.por.fl_str_mv |
Antifúngicos Biofilmes Candida Nanocarreadores Nanopartículas de óxido de ferro |
topic |
Antifúngicos Biofilmes Candida Nanocarreadores Nanopartículas de óxido de ferro Antifungals Biofilms Candida Nanocarriers Iron oxide nanoparticles CIENCIAS DA SAUDE::ODONTOLOGIA |
dc.subject.eng.fl_str_mv |
Antifungals Biofilms Candida Nanocarriers Iron oxide nanoparticles |
dc.subject.cnpq.fl_str_mv |
CIENCIAS DA SAUDE::ODONTOLOGIA |
description |
Resistance of Candida species to conventional therapies has motivated the development of antifungal nanocarriers based on iron oxide nanoparticles (IONPs) coated with chitosan (CS). This study evaluated the effects of IONPs-CS as carriers of miconazole (MCZ) or fluconazole (FLZ) on microcosm biofilms. Pooled saliva from two healthy volunteers supplemented with Candida albicans and Candida glabrata was the inoculum for biofilm formation. Biofilms were formed for 96 h on coverslips using the Amsterdam Active Attachment model, followed by 24 h treatment with nanocarriers containing different concentrations of each antifungal (78 and 156 µg/mL). MCZ or FLZ (156 µg/mL), and untreated biofilms were considered as controls. Anti-biofilm effects were evaluated by enumeration of colony-forming units (CFUs), composition of the extracellular matrix, lactic acid production, and structure and live/dead biofilm cells (confocal laser scanning microscopy - CLSM). Data were analyzed by 1-way ANOVA and Fisher LSD’s test (α = 0.05). IONPs-CS carrying MCZ or FLZ were the most effective treatments in reducing CFUs compared to either antifungal agent alone for C. albicans and MCZ for C. glabrata. Significant reductions in mutans streptococci and Lactobacillus spp. were shown, though mainly for the MCZ nanocarrier. Antifungals and their nanocarriers also showed significantly higher proportions of dead cells compared to untreated biofilm by CLSM (p < 0.001), and promoted significant reductions in lactic acid, while simultaneously showing increases in some components of the extracellular matrix. These findings reinforce the use of nanocarriers as effective alternatives to fight oral fungal infections. |
publishDate |
2021 |
dc.date.issued.fl_str_mv |
2021-03-19 |
dc.date.accessioned.fl_str_mv |
2022-04-07T18:12:28Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
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masterThesis |
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publishedVersion |
dc.identifier.citation.fl_str_mv |
CALDEIRÃO, Anne Caroline Morais. Efeito de nanocarreadores de drogas antifúngicas sobre espécies de Candida em biofilmes microcosmos. 2021. Dissertação (Mestrado em Odontologia) - Universidade do Oeste Paulista, Presidente Prudente, SP, 2021. |
dc.identifier.uri.fl_str_mv |
http://bdtd.unoeste.br:8080/jspui/handle/jspui/1393 |
identifier_str_mv |
CALDEIRÃO, Anne Caroline Morais. Efeito de nanocarreadores de drogas antifúngicas sobre espécies de Candida em biofilmes microcosmos. 2021. Dissertação (Mestrado em Odontologia) - Universidade do Oeste Paulista, Presidente Prudente, SP, 2021. |
url |
http://bdtd.unoeste.br:8080/jspui/handle/jspui/1393 |
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por |
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por |
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Mestrado em Odontologia |
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Universidade do Oeste Paulista |
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