Efeito de diferentes doses de L-arginina em ratos após a quimioterapia com 5-Fluorouracil

Detalhes bibliográficos
Ano de defesa: 2017
Autor(a) principal: ARAÚJO, Eloisa Ortega Nazário de lattes
Orientador(a): REIS, Luis Souza Lima de Souza lattes
Banca de defesa: SANCHES, Osimar de Carvalho lattes, BREMER NETO, Hermann lattes
Tipo de documento: Dissertação
Tipo de acesso: Acesso aberto
Idioma: por
Instituição de defesa: Universidade do Oeste Paulista
Programa de Pós-Graduação: Mestrado em Ciência Animal
Departamento: Mestrado em Ciência Animal
País: Brasil
Palavras-chave em Português:
Suplementação alimentar, micronúcleo, aminoácido, cromossomo
Palavras-chave em Inglês:
Food supplementation, micronucleus, amino acid, chromosome
Área do conhecimento CNPq:
CIENCIAS AGRARIAS::MEDICINA VETERINARIA
Link de acesso: http://bdtd.unoeste.br:8080/jspui/handle/jspui/1039
Resumo: L-arginine has been used as a dietary supplement to minimize the side effects of chemotherapy, but it is not fully elucidated its actions and nor the ideal dose that can minimize the side effects. The objective of this study was to evaluate the effect of L-arginine supplementation on the formation of micronuclei in 70 Wistar rats after chemotherapy with 5-FU divided into 7 batches (10 male rats weighing 200 g/batch): LC water ad libitum; LArg2% and LArg4% mice fed commercial feed and 2% and 4% of L-arginine in water ad libitum, respectively; LC rats fed commercial feed, water ad libitum and a dose of 50 mg cyclophosphamide/kg; L5-FU rats fed commercial feed, water ad libitum and a dose of 200 mg of 5-FU/kg was applied; LArg2%+5-FU and LArg4%+5-FU rats fed commercial feed, 2% and 4% L-arginine were added in water ad libitum, respectively, and a dose of 200 mg of 5-FU/Kg. The mice were sacrificed 72 hours after the application of the chemotherapeutics to evaluate the formation of micronuclei. In the data analysis, one-way ANOVA was applied and followed by the Tukey test at 5%. Mice supplemented ad libitum with 2% and 4% L-arginine showed a reduction (P < 0.05) in the formation of micronuclei in bone marrow cells after chemotherapy, indicating that these supplements minimize the mutagenic effect of 5-FU.
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spelling REIS, Luis Souza Lima de Souza11079865837http://lattes.cnpq.br/3555257260011109SANCHES, Osimar de Carvalho39447960200http://lattes.cnpq.br/2472163128986353BREMER NETO, Hermann46621695934http://lattes.cnpq.br/653268465483717337780299830http://lattes.cnpq.br/0037213367230070ARAÚJO, Eloisa Ortega Nazário de2017-08-15T19:37:52Z2017-05-23ARAÚJO, Eloisa Ortega Nazário de. Efeito de diferentes doses de L-arginina em ratos após a quimioterapia com 5-Fluorouracil. 2017. 35 f. Dissertação (Mestrado em Ciência Animal) - Universidade do Oeste Paulista, Presidente Prudente, 2017.http://bdtd.unoeste.br:8080/jspui/handle/jspui/1039L-arginine has been used as a dietary supplement to minimize the side effects of chemotherapy, but it is not fully elucidated its actions and nor the ideal dose that can minimize the side effects. The objective of this study was to evaluate the effect of L-arginine supplementation on the formation of micronuclei in 70 Wistar rats after chemotherapy with 5-FU divided into 7 batches (10 male rats weighing 200 g/batch): LC water ad libitum; LArg2% and LArg4% mice fed commercial feed and 2% and 4% of L-arginine in water ad libitum, respectively; LC rats fed commercial feed, water ad libitum and a dose of 50 mg cyclophosphamide/kg; L5-FU rats fed commercial feed, water ad libitum and a dose of 200 mg of 5-FU/kg was applied; LArg2%+5-FU and LArg4%+5-FU rats fed commercial feed, 2% and 4% L-arginine were added in water ad libitum, respectively, and a dose of 200 mg of 5-FU/Kg. The mice were sacrificed 72 hours after the application of the chemotherapeutics to evaluate the formation of micronuclei. In the data analysis, one-way ANOVA was applied and followed by the Tukey test at 5%. Mice supplemented ad libitum with 2% and 4% L-arginine showed a reduction (P < 0.05) in the formation of micronuclei in bone marrow cells after chemotherapy, indicating that these supplements minimize the mutagenic effect of 5-FU.A suplementação com L-arginina tem sido utilizada para minimizar os efeitos colaterais da quimioterapia, no entanto, ainda não está totalmente elucidado seus benefícios e nem determinado uma dose ideal de suplementação que pode minimizar esses efeitos. Objetivou avaliar o efeito da suplementação com L-arginina na formação de micronúcleos em 70 ratos Wistar após a aplicação da 5-FU. Estes ratos foram divididos em sete lotes (10 ratos/lote): Lc ratos alimentados com ração comercial e água ad libitum; LArg2% e LArg4% ratos alimentados com ração comercial e adicionou-se 2% e 4% de L-arginina na água ad libitum, respectivamente; Lciclo ratos alimentados com ração comercial, água ad libitum e aplicou-se uma dose de 50 mg de ciclofosfamida/Kg; L5-FU ratos alimentados com ração comercial, água ad libitum e aplicou-se uma dose de 200 mg de 5-FU/Kg; LArg2%+5-FU e LArg4%+5-FU ratos alimentados com ração comercial, adicionou-se 2% e 4% de L-arginina na água ad libitum, respectivamente, e aplicou-se uma dose de 200 mg de 5-FU/Kg. A contagem de micronúcleos nos eritrócitos policromáticos dos ratos foi realizada 72 horas após a aplicação dos quimioterápicos. Os resultados obtidos foram analisados pela ANOVA one-way seguido do teste de Tukey a 5%. Os ratos dos lotes LArg2%+5-FU e LArg4%+5-FU apresentaram redução (P < 0,05) na formação de micronúcleos nos eritrócitos policromáticos após a quimioterapia, indicando que estas suplementações com L-arginina minimizou o efeito mutagênico da 5-FU.Submitted by Adriana Martinez (amartinez@unoeste.br) on 2017-08-15T19:37:52Z No. of bitstreams: 2 license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) Eloisa.pdf: 351801 bytes, checksum: 5d8bb006aa699c1f75f27ffc6a85479b (MD5)Made available in DSpace on 2017-08-15T19:37:52Z (GMT). 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dc.title.por.fl_str_mv Efeito de diferentes doses de L-arginina em ratos após a quimioterapia com 5-Fluorouracil
dc.title.alternative.eng.fl_str_mv Effect of different doses of L-arginine in rats after chemotherapy with 5-Fluorouracil
title Efeito de diferentes doses de L-arginina em ratos após a quimioterapia com 5-Fluorouracil
spellingShingle Efeito de diferentes doses de L-arginina em ratos após a quimioterapia com 5-Fluorouracil
ARAÚJO, Eloisa Ortega Nazário de
Suplementação alimentar, micronúcleo, aminoácido, cromossomo
Food supplementation, micronucleus, amino acid, chromosome
CIENCIAS AGRARIAS::MEDICINA VETERINARIA
title_short Efeito de diferentes doses de L-arginina em ratos após a quimioterapia com 5-Fluorouracil
title_full Efeito de diferentes doses de L-arginina em ratos após a quimioterapia com 5-Fluorouracil
title_fullStr Efeito de diferentes doses de L-arginina em ratos após a quimioterapia com 5-Fluorouracil
title_full_unstemmed Efeito de diferentes doses de L-arginina em ratos após a quimioterapia com 5-Fluorouracil
title_sort Efeito de diferentes doses de L-arginina em ratos após a quimioterapia com 5-Fluorouracil
author ARAÚJO, Eloisa Ortega Nazário de
author_facet ARAÚJO, Eloisa Ortega Nazário de
author_role author
dc.contributor.advisor1.fl_str_mv REIS, Luis Souza Lima de Souza
dc.contributor.advisor1ID.fl_str_mv 11079865837
dc.contributor.advisor1Lattes.fl_str_mv http://lattes.cnpq.br/3555257260011109
dc.contributor.referee1.fl_str_mv SANCHES, Osimar de Carvalho
dc.contributor.referee1ID.fl_str_mv 39447960200
dc.contributor.referee1Lattes.fl_str_mv http://lattes.cnpq.br/2472163128986353
dc.contributor.referee2.fl_str_mv BREMER NETO, Hermann
dc.contributor.referee2ID.fl_str_mv 46621695934
dc.contributor.referee2Lattes.fl_str_mv http://lattes.cnpq.br/6532684654837173
dc.contributor.authorID.fl_str_mv 37780299830
dc.contributor.authorLattes.fl_str_mv http://lattes.cnpq.br/0037213367230070
dc.contributor.author.fl_str_mv ARAÚJO, Eloisa Ortega Nazário de
contributor_str_mv REIS, Luis Souza Lima de Souza
SANCHES, Osimar de Carvalho
BREMER NETO, Hermann
dc.subject.por.fl_str_mv Suplementação alimentar, micronúcleo, aminoácido, cromossomo
topic Suplementação alimentar, micronúcleo, aminoácido, cromossomo
Food supplementation, micronucleus, amino acid, chromosome
CIENCIAS AGRARIAS::MEDICINA VETERINARIA
dc.subject.eng.fl_str_mv Food supplementation, micronucleus, amino acid, chromosome
dc.subject.cnpq.fl_str_mv CIENCIAS AGRARIAS::MEDICINA VETERINARIA
description L-arginine has been used as a dietary supplement to minimize the side effects of chemotherapy, but it is not fully elucidated its actions and nor the ideal dose that can minimize the side effects. The objective of this study was to evaluate the effect of L-arginine supplementation on the formation of micronuclei in 70 Wistar rats after chemotherapy with 5-FU divided into 7 batches (10 male rats weighing 200 g/batch): LC water ad libitum; LArg2% and LArg4% mice fed commercial feed and 2% and 4% of L-arginine in water ad libitum, respectively; LC rats fed commercial feed, water ad libitum and a dose of 50 mg cyclophosphamide/kg; L5-FU rats fed commercial feed, water ad libitum and a dose of 200 mg of 5-FU/kg was applied; LArg2%+5-FU and LArg4%+5-FU rats fed commercial feed, 2% and 4% L-arginine were added in water ad libitum, respectively, and a dose of 200 mg of 5-FU/Kg. The mice were sacrificed 72 hours after the application of the chemotherapeutics to evaluate the formation of micronuclei. In the data analysis, one-way ANOVA was applied and followed by the Tukey test at 5%. Mice supplemented ad libitum with 2% and 4% L-arginine showed a reduction (P < 0.05) in the formation of micronuclei in bone marrow cells after chemotherapy, indicating that these supplements minimize the mutagenic effect of 5-FU.
publishDate 2017
dc.date.accessioned.fl_str_mv 2017-08-15T19:37:52Z
dc.date.issued.fl_str_mv 2017-05-23
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dc.identifier.citation.fl_str_mv ARAÚJO, Eloisa Ortega Nazário de. Efeito de diferentes doses de L-arginina em ratos após a quimioterapia com 5-Fluorouracil. 2017. 35 f. Dissertação (Mestrado em Ciência Animal) - Universidade do Oeste Paulista, Presidente Prudente, 2017.
dc.identifier.uri.fl_str_mv http://bdtd.unoeste.br:8080/jspui/handle/jspui/1039
identifier_str_mv ARAÚJO, Eloisa Ortega Nazário de. Efeito de diferentes doses de L-arginina em ratos após a quimioterapia com 5-Fluorouracil. 2017. 35 f. Dissertação (Mestrado em Ciência Animal) - Universidade do Oeste Paulista, Presidente Prudente, 2017.
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