Limiar de detecção de larvas de Toxocara canis em leite bovino experimentalmente contaminado
Ano de defesa: | 2015 |
---|---|
Autor(a) principal: | |
Orientador(a): | |
Banca de defesa: | , |
Tipo de documento: | Dissertação |
Tipo de acesso: | Acesso aberto |
Idioma: | por |
Instituição de defesa: |
Universidade do Oeste Paulista
|
Programa de Pós-Graduação: |
Mestrado em Ciência Animal
|
Departamento: |
Ciências Agrárias
|
País: |
BR
|
Palavras-chave em Português: | |
Palavras-chave em Inglês: | |
Área do conhecimento CNPq: | |
Link de acesso: | http://bdtd.unoeste.br:8080/tede/handle/tede/317 |
Resumo: | This study aimed to evaluate the limit of detection of Toxocara canis larvae in experimentally contaminated commercial bovine milk, based on a centrifuge-sedimentation technique. Firstly, bovine milk (integral and skim) samples were contaminated with 50 T. canis larvae, in order to evaluate the interference of milk fat on the recovery of the larvae. The second step of the study was to verify the influence of formalin 10% (100 µL), ether (100 µL) and combination of both solutions. The third step consisted of studying the limit of detection of larvae, using the best solution (step 2) for degreasing the milk. Samples were contaminated with aliquots of 1; 5; 10; 25 and 50 larvae. For each milk sample (1.0 mL), 15 repetitions were analyzed. It was observed that the recovery of the larvae from the skim milk was higher (p= 0.0031) than those from the integral milk. There was no significant difference (p= 0.5681) regarding the percentage of recovered larvae in comparing the degreasing solutions. Nevertheless, the formalin-ether combination was more efficient to recover the larvae (73.1%) in comparison to the ether (71.9%), the pure integral milk (70.0%) as well as the formalin (67.6%). Concerned to the limit of detection (using formalin-ether), all the samples contaminated with 5; 10; 25 and 50 larvae were positive (minimum: 62.7%). The samples contaminated with a single larva, 66.7% were positive. The technique may be useful for recovering Toxocara spp. larvae in milk samples of a wide range of animal species, either naturally or experimentally contaminated. |
id |
UOES_c20951ba36ce0ebc4bb259fa2a8740fb |
---|---|
oai_identifier_str |
oai:bdtd.unoeste.br:tede/317 |
network_acronym_str |
UOES |
network_name_str |
Biblioteca Digital de Teses e Dissertações da UNOESTE |
repository_id_str |
|
spelling |
Santarém, Vamilton álvaresCPF:31873065515http://lattes.cnpq.br/2204972437815213Gonçalves, Daniela DibCPF:27754042860http://lattes.cnpq.br/9047414677271009Nogueira, Rosa Maria BarilliCPF:06991647888http://lattes.cnpq.br/7556647746768615CPF:32678794857http://lattes.cnpq.br/3546829235685725Pereira, Edlayne Larissa Gretter Machado2016-01-26T18:55:43Z2015-08-042015-03-27PEREIRA, Edlayne Larissa Gretter Machado. Limit of detection of Toxocara canis larvae in experimentally contaminated bovine milk. 2015. 32 f. Dissertação (Mestrado em Ciências Agrárias) - Universidade do Oeste Paulista, Presidente Prudente, 2015.http://bdtd.unoeste.br:8080/tede/handle/tede/317This study aimed to evaluate the limit of detection of Toxocara canis larvae in experimentally contaminated commercial bovine milk, based on a centrifuge-sedimentation technique. Firstly, bovine milk (integral and skim) samples were contaminated with 50 T. canis larvae, in order to evaluate the interference of milk fat on the recovery of the larvae. The second step of the study was to verify the influence of formalin 10% (100 µL), ether (100 µL) and combination of both solutions. The third step consisted of studying the limit of detection of larvae, using the best solution (step 2) for degreasing the milk. Samples were contaminated with aliquots of 1; 5; 10; 25 and 50 larvae. For each milk sample (1.0 mL), 15 repetitions were analyzed. It was observed that the recovery of the larvae from the skim milk was higher (p= 0.0031) than those from the integral milk. There was no significant difference (p= 0.5681) regarding the percentage of recovered larvae in comparing the degreasing solutions. Nevertheless, the formalin-ether combination was more efficient to recover the larvae (73.1%) in comparison to the ether (71.9%), the pure integral milk (70.0%) as well as the formalin (67.6%). Concerned to the limit of detection (using formalin-ether), all the samples contaminated with 5; 10; 25 and 50 larvae were positive (minimum: 62.7%). The samples contaminated with a single larva, 66.7% were positive. The technique may be useful for recovering Toxocara spp. larvae in milk samples of a wide range of animal species, either naturally or experimentally contaminated.O objetivo do estudo foi avaliar o limiar de detecção de larvas de Toxocara canis em leite bovino comercial contaminado experimentalmente, a partir de uma técnica de centrifugo-sedimentação. Primeiramente, amostras de leite bovino comercial (integral e desnatado) foram contaminadas com 50 larvas de T. canis, para avaliação da interferência da gordura sobre a recuperação das larvas. Em uma segunda etapa, foi avaliada a ação de formalina 10% (100 µL), éter (100 µL) e combinação das soluções. A terceira etapa consistiu da verificação do limite de detecção de larvas, com uso da melhor solução desengordurante (etapa 2), em amostras de leite (1,0 mL) contendo 1; 5; 10; 25 e 50 larvas. Para cada análise de detecção do leite (1,0 mL), foram realizadas 15 repetições. Foi observado que o percentual de recuperação de larvas no leite desnatado foi significativamente maior (p= 0,0031) que o observado no leite integral. Na comparação das soluções, não houve diferença significativa (p= 0,5681) no percentual de larvas recuperadas. Entretanto, houve uma maior recuperação quando do emprego da combinação de formol-éter (73,1%) em relação ao éter (71,9%), leite integral puro (70,0%) e ao formol (67,6%). Em relação ao limite de detecção, com uso de formalina-éter, todas as alíquotas apresentaram resultado positivo (mínimo de 62,7% de larvas recuperadas), para as amostras com 5, 10, 25 e 50 larvas. Nas amostras contaminadas com uma larva, 66,7% das alíquotas foram positivas. A técnica apresentada poderá servir para recuperação de larvas de Toxocara spp. em amostras de leite de várias espécies animais, contaminados naturalmente ou experimentalmente.Made available in DSpace on 2016-01-26T18:55:43Z (GMT). No. of bitstreams: 1 Edlayne Larissa Gretter Machado Periera.pdf: 352357 bytes, checksum: a92ace5bfa90617b05e3cdaa7e4de894 (MD5) Previous issue date: 2015-03-27application/pdfhttp://bdtd.unoeste.br:8080/tede/retrieve/847/Edlayne%20Larissa%20Gretter%20Machado%20Periera.pdf.jpgporUniversidade do Oeste PaulistaMestrado em Ciência AnimalUNOESTEBRCiências Agráriastoxocaríaserecuperação de larvasdiagnósticotransmissãotoxocariasisrecovery of larvaediagnosistransmissionCNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIALimiar de detecção de larvas de Toxocara canis em leite bovino experimentalmente contaminadoLimit of detection of Toxocara canis larvae in experimentally contaminated bovine milkinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesis1886725639743614135500info:eu-repo/semantics/openAccessreponame:Biblioteca Digital de Teses e Dissertações da UNOESTEinstname:Universidade do Oeste Paulista (UNOESTE)instacron:UNOESTEORIGINALEdlayne Larissa Gretter Machado Periera.pdfapplication/pdf352357http://bdtd.unoeste.br:8080/tede/bitstream/tede/317/1/Edlayne+Larissa+Gretter+Machado+Periera.pdfa92ace5bfa90617b05e3cdaa7e4de894MD51THUMBNAILEdlayne Larissa Gretter Machado Periera.pdf.jpgEdlayne Larissa Gretter Machado Periera.pdf.jpgimage/jpeg2179http://bdtd.unoeste.br:8080/tede/bitstream/tede/317/2/Edlayne+Larissa+Gretter+Machado+Periera.pdf.jpg01a3bf5869d25756f643066aef98b98bMD52tede/3172016-01-27 01:04:03.402oai:bdtd.unoeste.br:tede/317Biblioteca Digital de Teses e Dissertaçõeshttp://bdtd.unoeste.br:8080/jspui/PUBhttp://bdtd.unoeste.br:8080/oai/requestbdtd@unoeste.bropendoar:2016-01-27T03:04:03Biblioteca Digital de Teses e Dissertações da UNOESTE - Universidade do Oeste Paulista (UNOESTE)false |
dc.title.por.fl_str_mv |
Limiar de detecção de larvas de Toxocara canis em leite bovino experimentalmente contaminado |
dc.title.alternative.eng.fl_str_mv |
Limit of detection of Toxocara canis larvae in experimentally contaminated bovine milk |
title |
Limiar de detecção de larvas de Toxocara canis em leite bovino experimentalmente contaminado |
spellingShingle |
Limiar de detecção de larvas de Toxocara canis em leite bovino experimentalmente contaminado Pereira, Edlayne Larissa Gretter Machado toxocaríase recuperação de larvas diagnóstico transmissão toxocariasis recovery of larvae diagnosis transmission CNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIA |
title_short |
Limiar de detecção de larvas de Toxocara canis em leite bovino experimentalmente contaminado |
title_full |
Limiar de detecção de larvas de Toxocara canis em leite bovino experimentalmente contaminado |
title_fullStr |
Limiar de detecção de larvas de Toxocara canis em leite bovino experimentalmente contaminado |
title_full_unstemmed |
Limiar de detecção de larvas de Toxocara canis em leite bovino experimentalmente contaminado |
title_sort |
Limiar de detecção de larvas de Toxocara canis em leite bovino experimentalmente contaminado |
author |
Pereira, Edlayne Larissa Gretter Machado |
author_facet |
Pereira, Edlayne Larissa Gretter Machado |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Santarém, Vamilton álvares |
dc.contributor.advisor1ID.fl_str_mv |
CPF:31873065515 |
dc.contributor.advisor1Lattes.fl_str_mv |
http://lattes.cnpq.br/2204972437815213 |
dc.contributor.referee1.fl_str_mv |
Gonçalves, Daniela Dib |
dc.contributor.referee1ID.fl_str_mv |
CPF:27754042860 |
dc.contributor.referee1Lattes.fl_str_mv |
http://lattes.cnpq.br/9047414677271009 |
dc.contributor.referee2.fl_str_mv |
Nogueira, Rosa Maria Barilli |
dc.contributor.referee2ID.fl_str_mv |
CPF:06991647888 |
dc.contributor.referee2Lattes.fl_str_mv |
http://lattes.cnpq.br/7556647746768615 |
dc.contributor.authorID.fl_str_mv |
CPF:32678794857 |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/3546829235685725 |
dc.contributor.author.fl_str_mv |
Pereira, Edlayne Larissa Gretter Machado |
contributor_str_mv |
Santarém, Vamilton álvares Gonçalves, Daniela Dib Nogueira, Rosa Maria Barilli |
dc.subject.por.fl_str_mv |
toxocaríase recuperação de larvas diagnóstico transmissão |
topic |
toxocaríase recuperação de larvas diagnóstico transmissão toxocariasis recovery of larvae diagnosis transmission CNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIA |
dc.subject.eng.fl_str_mv |
toxocariasis recovery of larvae diagnosis transmission |
dc.subject.cnpq.fl_str_mv |
CNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIA |
description |
This study aimed to evaluate the limit of detection of Toxocara canis larvae in experimentally contaminated commercial bovine milk, based on a centrifuge-sedimentation technique. Firstly, bovine milk (integral and skim) samples were contaminated with 50 T. canis larvae, in order to evaluate the interference of milk fat on the recovery of the larvae. The second step of the study was to verify the influence of formalin 10% (100 µL), ether (100 µL) and combination of both solutions. The third step consisted of studying the limit of detection of larvae, using the best solution (step 2) for degreasing the milk. Samples were contaminated with aliquots of 1; 5; 10; 25 and 50 larvae. For each milk sample (1.0 mL), 15 repetitions were analyzed. It was observed that the recovery of the larvae from the skim milk was higher (p= 0.0031) than those from the integral milk. There was no significant difference (p= 0.5681) regarding the percentage of recovered larvae in comparing the degreasing solutions. Nevertheless, the formalin-ether combination was more efficient to recover the larvae (73.1%) in comparison to the ether (71.9%), the pure integral milk (70.0%) as well as the formalin (67.6%). Concerned to the limit of detection (using formalin-ether), all the samples contaminated with 5; 10; 25 and 50 larvae were positive (minimum: 62.7%). The samples contaminated with a single larva, 66.7% were positive. The technique may be useful for recovering Toxocara spp. larvae in milk samples of a wide range of animal species, either naturally or experimentally contaminated. |
publishDate |
2015 |
dc.date.available.fl_str_mv |
2015-08-04 |
dc.date.issued.fl_str_mv |
2015-03-27 |
dc.date.accessioned.fl_str_mv |
2016-01-26T18:55:43Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
PEREIRA, Edlayne Larissa Gretter Machado. Limit of detection of Toxocara canis larvae in experimentally contaminated bovine milk. 2015. 32 f. Dissertação (Mestrado em Ciências Agrárias) - Universidade do Oeste Paulista, Presidente Prudente, 2015. |
dc.identifier.uri.fl_str_mv |
http://bdtd.unoeste.br:8080/tede/handle/tede/317 |
identifier_str_mv |
PEREIRA, Edlayne Larissa Gretter Machado. Limit of detection of Toxocara canis larvae in experimentally contaminated bovine milk. 2015. 32 f. Dissertação (Mestrado em Ciências Agrárias) - Universidade do Oeste Paulista, Presidente Prudente, 2015. |
url |
http://bdtd.unoeste.br:8080/tede/handle/tede/317 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.program.fl_str_mv |
1886725639743614135 |
dc.relation.confidence.fl_str_mv |
500 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade do Oeste Paulista |
dc.publisher.program.fl_str_mv |
Mestrado em Ciência Animal |
dc.publisher.initials.fl_str_mv |
UNOESTE |
dc.publisher.country.fl_str_mv |
BR |
dc.publisher.department.fl_str_mv |
Ciências Agrárias |
publisher.none.fl_str_mv |
Universidade do Oeste Paulista |
dc.source.none.fl_str_mv |
reponame:Biblioteca Digital de Teses e Dissertações da UNOESTE instname:Universidade do Oeste Paulista (UNOESTE) instacron:UNOESTE |
instname_str |
Universidade do Oeste Paulista (UNOESTE) |
instacron_str |
UNOESTE |
institution |
UNOESTE |
reponame_str |
Biblioteca Digital de Teses e Dissertações da UNOESTE |
collection |
Biblioteca Digital de Teses e Dissertações da UNOESTE |
bitstream.url.fl_str_mv |
http://bdtd.unoeste.br:8080/tede/bitstream/tede/317/1/Edlayne+Larissa+Gretter+Machado+Periera.pdf http://bdtd.unoeste.br:8080/tede/bitstream/tede/317/2/Edlayne+Larissa+Gretter+Machado+Periera.pdf.jpg |
bitstream.checksum.fl_str_mv |
a92ace5bfa90617b05e3cdaa7e4de894 01a3bf5869d25756f643066aef98b98b |
bitstream.checksumAlgorithm.fl_str_mv |
MD5 MD5 |
repository.name.fl_str_mv |
Biblioteca Digital de Teses e Dissertações da UNOESTE - Universidade do Oeste Paulista (UNOESTE) |
repository.mail.fl_str_mv |
bdtd@unoeste.br |
_version_ |
1785359465408626688 |