Efeito da terapia com Laser de Baixa Intensidade (LBI) em modelo experimental de inflamação alérgica pulmonar induzida por House Dust Mite (HDM) - estudo de dosimetria
Ano de defesa: | 2018 |
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Autor(a) principal: | |
Orientador(a): | |
Banca de defesa: | , , , , |
Tipo de documento: | Tese |
Tipo de acesso: | Acesso aberto |
Idioma: | por |
Instituição de defesa: |
Universidade Nove de Julho
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Programa de Pós-Graduação: |
Programa de Pós-Graduação em Biofotônica Aplicada às Ciências da Saúde
|
Departamento: |
Saúde
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País: |
Brasil
|
Palavras-chave em Português: | |
Palavras-chave em Inglês: | |
Área do conhecimento CNPq: | |
Link de acesso: | http://bibliotecatede.uninove.br/handle/tede/2666 |
Resumo: | Asthma is characterized by chronic inflammation in the airways. It presents high prevalence, economic and social cost. Several models have been proposed aiming the discovery of new therapies. Low Level Laser Therapy (LLLT) is relatively new and effective, of very low cost, with no side effects. As for laser therapy, there is still no consensus on the optimal dose to be used. In this sense, the objective of the present study was to evaluate the effect of LLLT with different energies on chronic lung inflammation in an experimental model of asthma induced by House Dust Mite (HDM). We used Balb/c mice, divided into 10 groups: Basal, HDM, Laser (1J, 3J, 5J and 7,5J) and HDM+Laser (1J, 3J, 5J and 7,5J). The LLLT used was diode (660 nm, 100 mW) and for each energy, we used different times (10s, 30s, 50s and 75s) in three points: trachea, left lobe and right. For the experimental model, the animals received orotracheal administration of 100ug/animal HDM on days 0, 14, followed by 3 weekly administrations by day 56. After 24 hours, we studied functional and structural parameters, such as pulmonary inflammation through bronchoalveolar lavage fluid (BALF), total and differential cell count, quantification of CD11b, LY6G, Syglec F, CD3+, CD4+ and CD8+ in the lung, the percentage of 7AAD and ANEXINA V, as well as CD4+, STAT6+, GATA3+ and IL-4+ lymphocytes in BALF by flow cytometry, cytokines in BALF and pulmonary homogenate (IL-4, IL-5, IL-10 and IL-13) by ELISA, morphometric analysis of eosinophilic peribronchial infiltrate (LUNA), mucus (PAS), collagen (PSR), bronchoconstriction index, bronchial and muscular thickening (HE), pulmonary mechanics (pulmonary elastance) and pulmonary reactivity by the Power Lab method. Results showed that LLLT was able to modulate observed pulmonary inflammation by reducing the number of cells in the BALF, as well as reducing the percentage of neutrophils, eosinophils and T lymphocytes (CD4+ and CD8+). In addition, it decreased CD4+ STAT6+ and GATA3+ lymphocytes, as well as apoptosis and cell necrosis. On the other hand, laser therapy increased the percentage of macrophages and IL-10 and reduced levels of IL-4, IL-5 and IL-13 in the BALF and pulmonary homogenate. LLLT was able to reduce mucus production. Regarding the structural alterations, we observed a significant reduction in the quantification of peribronchial eosinophils, collagen deposition, bronchoconstriction index, bronchial and muscular 9 thickening in the airways, after LLLT treatment. Finally, in functional analysis, the evaluation of tracheal reactivity to methacholine and evaluation of pulmonary mechanics presented a decrease in all groups submitted to laser therapy. We conclude that the use of laser therapy in the treatment of chronic inflammation of the airways attenuated the inflammatory process, functional parameters and pulmonary structure. We emphasize in general that the 3J laser presented better results. Thus, photobiomodulation can be considered a promising tool for the treatment of chronic pulmonary allergic inflammation observed in asthma. |
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Oliveira, Ana Paula Ligeiro dehttp://lattes.cnpq.br/5700855351581023Oliveira, Ana Paula Ligeiro dehttp://lattes.cnpq.br/5700855351581023Franco, Adriana Lino dos Santoshttp://lattes.cnpq.br/7295388304888384Marcos, Rodrigo Labathttp://lattes.cnpq.br/2964112163504080Carvalho, Flávio Aimbire Soares dehttp://lattes.cnpq.br/8216583445045638Romanholo, Beatriz Mangueira Saraivahttp://lattes.cnpq.br/6037246361594215http://lattes.cnpq.br/6492329271471517Oliveira, Nicole Cristine Rigonato de2021-11-04T17:45:08Z2018-07-31Oliveira, Nicole Cristine Rigonato de. Efeito da terapia com Laser de Baixa Intensidade (LBI) em modelo experimental de inflamação alérgica pulmonar induzida por House Dust Mite (HDM) - estudo de dosimetria. 2018. 96 f. Tese( Programa de Pós-Graduação em Biofotônica Aplicada às Ciências da Saúde) - Universidade Nove de Julho, São Paulo.http://bibliotecatede.uninove.br/handle/tede/2666Asthma is characterized by chronic inflammation in the airways. It presents high prevalence, economic and social cost. Several models have been proposed aiming the discovery of new therapies. Low Level Laser Therapy (LLLT) is relatively new and effective, of very low cost, with no side effects. As for laser therapy, there is still no consensus on the optimal dose to be used. In this sense, the objective of the present study was to evaluate the effect of LLLT with different energies on chronic lung inflammation in an experimental model of asthma induced by House Dust Mite (HDM). We used Balb/c mice, divided into 10 groups: Basal, HDM, Laser (1J, 3J, 5J and 7,5J) and HDM+Laser (1J, 3J, 5J and 7,5J). The LLLT used was diode (660 nm, 100 mW) and for each energy, we used different times (10s, 30s, 50s and 75s) in three points: trachea, left lobe and right. For the experimental model, the animals received orotracheal administration of 100ug/animal HDM on days 0, 14, followed by 3 weekly administrations by day 56. After 24 hours, we studied functional and structural parameters, such as pulmonary inflammation through bronchoalveolar lavage fluid (BALF), total and differential cell count, quantification of CD11b, LY6G, Syglec F, CD3+, CD4+ and CD8+ in the lung, the percentage of 7AAD and ANEXINA V, as well as CD4+, STAT6+, GATA3+ and IL-4+ lymphocytes in BALF by flow cytometry, cytokines in BALF and pulmonary homogenate (IL-4, IL-5, IL-10 and IL-13) by ELISA, morphometric analysis of eosinophilic peribronchial infiltrate (LUNA), mucus (PAS), collagen (PSR), bronchoconstriction index, bronchial and muscular thickening (HE), pulmonary mechanics (pulmonary elastance) and pulmonary reactivity by the Power Lab method. Results showed that LLLT was able to modulate observed pulmonary inflammation by reducing the number of cells in the BALF, as well as reducing the percentage of neutrophils, eosinophils and T lymphocytes (CD4+ and CD8+). In addition, it decreased CD4+ STAT6+ and GATA3+ lymphocytes, as well as apoptosis and cell necrosis. On the other hand, laser therapy increased the percentage of macrophages and IL-10 and reduced levels of IL-4, IL-5 and IL-13 in the BALF and pulmonary homogenate. LLLT was able to reduce mucus production. Regarding the structural alterations, we observed a significant reduction in the quantification of peribronchial eosinophils, collagen deposition, bronchoconstriction index, bronchial and muscular 9 thickening in the airways, after LLLT treatment. Finally, in functional analysis, the evaluation of tracheal reactivity to methacholine and evaluation of pulmonary mechanics presented a decrease in all groups submitted to laser therapy. We conclude that the use of laser therapy in the treatment of chronic inflammation of the airways attenuated the inflammatory process, functional parameters and pulmonary structure. We emphasize in general that the 3J laser presented better results. Thus, photobiomodulation can be considered a promising tool for the treatment of chronic pulmonary allergic inflammation observed in asthma.A asma é caracterizada por inflamação crônica das vias aéreas. Apresenta alta prevalência, custo econômico e social. Vários modelos têm sido propostos, objetivando a descoberta de novas terapias. A terapia com Laser de Baixa Intensidade (LBI) é relativamente nova e eficaz, de baixíssimo custo, sem efeitos colaterais. Quanto à laserterapia, ainda não existe um consenso sobre a dose ideal a ser utilizada. Nesse sentido, o objetivo do presente trabalho foi avaliar o efeito do LBI com diferentes energias sobre a inflamação pulmonar crônica em modelo experimental de asma induzida por House Dust Mite (HDM). Utilizamos camundongos Balb/c, divididos em 10 grupos: Basal, HDM, Laser (1J, 3J, 5J e 7,5J) e HDM+Laser (1J, 3J, 5J e 7,5J). O LBI utilizado foi o de diodo (660 nm, 100 mW) e para cada energia, utilizamos diferentes tempos (10s, 30s, 50s e 75s), em três pontos: traquéia, lobo esquerdo e direito. Para o modelo experimental, os animais receberam administração orotraqueal de 100ug/animal de HDM nos dias 0, 14, seguidos de 3 administrações semanais até o dia 56. Após 24 horas, estudamos parâmetros funcionais e estruturais, como a inflamação pulmonar através do fluído do lavado broncoalveolar (FLBA), contagem de células totais e diferenciais, quantificação de CD11b, LY6G, Siglec-F, CD3+, CD4+ e CD8+ no pulmão, a porcentagem de 7AAD e ANEXINA V, bem como de linfócitos CD4+, STAT6+, GATA3+ e IL-4+ no FLBA por citometria de fluxo, citocinas no FLBA e homogenato pulmonar (IL-4, IL-5, IL-10 e IL-13) por ELISA, análise morfométrica do infiltrado peribrônquico eosinofílico (LUNA), de muco (PAS), colágeno (PSR), índice de broncoconstrição, espessamento brônquico e muscular (HE), mecânica pulmonar (elastância pulmonar) e reatividade pulmonar pelo método Power Lab. Os resultados evidenciaram que o LBI foi capaz de modular a inflamação pulmonar observado pela redução do número de células no FLBA, bem como reduziu a porcentagem de neutrófilos, eosinófilos e linfócitos T (CD4+ e CD8+). Além disso, diminuiu os linfócitos CD4+STAT 6+ e GATA3+, bem como apoptose e necrose celular. Por outro lado, a laserterapia aumentou a porcentagem de macrófagos e de IL-10 e reduziu os níveis de IL-4, IL-5 e IL-13 no FLBA e homogenato pulmonar. O LBI foi capaz de reduzir a produção de muco. Com relação às alterações estruturais, observamos importante redução na quantificação de eosinófilos peribrônquicos, deposição de 7 colágeno, índice de broncoconstrição, espessamento brônquico e muscular nas vias aéreas, após o tratamento com LBI. Por fim, em análise funcional, a avaliação da reatividade traqueal frente à metacolina e avaliação da mecânica pulmonar apresentou diminuição em todos os grupos submetidos à laserterapia. Concluímos assim, que o uso da laserterapia no tratamento da inflamação crônica das vias aéreas, atenuou o processo inflamatório, os parâmetros funcionais e estruturais pulmonares. Destacamos de maneira geral que o laser com 3J, apresentou melhores resultados. Dessa maneira, a fotobiomodulação pode ser considerada uma ferramenta promissora para o tratamento da inflamação alérgica pulmonar crônica, observada na asma.Submitted by Nadir Basilio (nadirsb@uninove.br) on 2021-11-04T17:45:08Z No. of bitstreams: 1 Nicole Cristine Rigonato de Oliveira.pdf: 4456662 bytes, checksum: 3a624e1a888b774a7945fffb2eaba24e (MD5)Made available in DSpace on 2021-11-04T17:45:08Z (GMT). No. of bitstreams: 1 Nicole Cristine Rigonato de Oliveira.pdf: 4456662 bytes, checksum: 3a624e1a888b774a7945fffb2eaba24e (MD5) Previous issue date: 2018-07-31application/pdfporUniversidade Nove de JulhoPrograma de Pós-Graduação em Biofotônica Aplicada às Ciências da SaúdeUNINOVEBrasilSaúdeasmalaser de baixa intensidadedosimetriaimunologiacitocinasasthmalow intensity laserdosimetryimmunologycytokinesCIENCIAS DA SAUDEEfeito da terapia com Laser de Baixa Intensidade (LBI) em modelo experimental de inflamação alérgica pulmonar induzida por House Dust Mite (HDM) - estudo de dosimetriainfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/doctoralThesis8765449414823306929600info:eu-repo/semantics/openAccessreponame:Biblioteca Digital de Teses e Dissertações da Uninoveinstname:Universidade Nove de Julho (UNINOVE)instacron:UNINOVEORIGINALNicole Cristine Rigonato de Oliveira.pdfNicole Cristine Rigonato de Oliveira.pdfapplication/pdf4456662http://localhost:8080/tede/bitstream/tede/2666/2/Nicole+Cristine+Rigonato+de+Oliveira.pdf3a624e1a888b774a7945fffb2eaba24eMD52LICENSElicense.txtlicense.txttext/plain; charset=utf-82165http://localhost:8080/tede/bitstream/tede/2666/1/license.txtbd3efa91386c1718a7f26a329fdcb468MD51tede/26662021-11-04 15:45:08.81oai:localhost: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Biblioteca Digital de Teses e Dissertaçõeshttp://bibliotecatede.uninove.br/PRIhttp://bibliotecatede.uninove.br/oai/requestbibliotecatede@uninove.br||bibliotecatede@uninove.bropendoar:2021-11-04T17:45:08Biblioteca Digital de Teses e Dissertações da Uninove - Universidade Nove de Julho (UNINOVE)false |
dc.title.por.fl_str_mv |
Efeito da terapia com Laser de Baixa Intensidade (LBI) em modelo experimental de inflamação alérgica pulmonar induzida por House Dust Mite (HDM) - estudo de dosimetria |
title |
Efeito da terapia com Laser de Baixa Intensidade (LBI) em modelo experimental de inflamação alérgica pulmonar induzida por House Dust Mite (HDM) - estudo de dosimetria |
spellingShingle |
Efeito da terapia com Laser de Baixa Intensidade (LBI) em modelo experimental de inflamação alérgica pulmonar induzida por House Dust Mite (HDM) - estudo de dosimetria Oliveira, Nicole Cristine Rigonato de asma laser de baixa intensidade dosimetria imunologia citocinas asthma low intensity laser dosimetry immunology cytokines CIENCIAS DA SAUDE |
title_short |
Efeito da terapia com Laser de Baixa Intensidade (LBI) em modelo experimental de inflamação alérgica pulmonar induzida por House Dust Mite (HDM) - estudo de dosimetria |
title_full |
Efeito da terapia com Laser de Baixa Intensidade (LBI) em modelo experimental de inflamação alérgica pulmonar induzida por House Dust Mite (HDM) - estudo de dosimetria |
title_fullStr |
Efeito da terapia com Laser de Baixa Intensidade (LBI) em modelo experimental de inflamação alérgica pulmonar induzida por House Dust Mite (HDM) - estudo de dosimetria |
title_full_unstemmed |
Efeito da terapia com Laser de Baixa Intensidade (LBI) em modelo experimental de inflamação alérgica pulmonar induzida por House Dust Mite (HDM) - estudo de dosimetria |
title_sort |
Efeito da terapia com Laser de Baixa Intensidade (LBI) em modelo experimental de inflamação alérgica pulmonar induzida por House Dust Mite (HDM) - estudo de dosimetria |
author |
Oliveira, Nicole Cristine Rigonato de |
author_facet |
Oliveira, Nicole Cristine Rigonato de |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Oliveira, Ana Paula Ligeiro de |
dc.contributor.advisor1Lattes.fl_str_mv |
http://lattes.cnpq.br/5700855351581023 |
dc.contributor.referee1.fl_str_mv |
Oliveira, Ana Paula Ligeiro de |
dc.contributor.referee1Lattes.fl_str_mv |
http://lattes.cnpq.br/5700855351581023 |
dc.contributor.referee2.fl_str_mv |
Franco, Adriana Lino dos Santos |
dc.contributor.referee2Lattes.fl_str_mv |
http://lattes.cnpq.br/7295388304888384 |
dc.contributor.referee3.fl_str_mv |
Marcos, Rodrigo Labat |
dc.contributor.referee3Lattes.fl_str_mv |
http://lattes.cnpq.br/2964112163504080 |
dc.contributor.referee4.fl_str_mv |
Carvalho, Flávio Aimbire Soares de |
dc.contributor.referee4Lattes.fl_str_mv |
http://lattes.cnpq.br/8216583445045638 |
dc.contributor.referee5.fl_str_mv |
Romanholo, Beatriz Mangueira Saraiva |
dc.contributor.referee5Lattes.fl_str_mv |
http://lattes.cnpq.br/6037246361594215 |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/6492329271471517 |
dc.contributor.author.fl_str_mv |
Oliveira, Nicole Cristine Rigonato de |
contributor_str_mv |
Oliveira, Ana Paula Ligeiro de Oliveira, Ana Paula Ligeiro de Franco, Adriana Lino dos Santos Marcos, Rodrigo Labat Carvalho, Flávio Aimbire Soares de Romanholo, Beatriz Mangueira Saraiva |
dc.subject.por.fl_str_mv |
asma laser de baixa intensidade dosimetria imunologia citocinas |
topic |
asma laser de baixa intensidade dosimetria imunologia citocinas asthma low intensity laser dosimetry immunology cytokines CIENCIAS DA SAUDE |
dc.subject.eng.fl_str_mv |
asthma low intensity laser dosimetry immunology cytokines |
dc.subject.cnpq.fl_str_mv |
CIENCIAS DA SAUDE |
description |
Asthma is characterized by chronic inflammation in the airways. It presents high prevalence, economic and social cost. Several models have been proposed aiming the discovery of new therapies. Low Level Laser Therapy (LLLT) is relatively new and effective, of very low cost, with no side effects. As for laser therapy, there is still no consensus on the optimal dose to be used. In this sense, the objective of the present study was to evaluate the effect of LLLT with different energies on chronic lung inflammation in an experimental model of asthma induced by House Dust Mite (HDM). We used Balb/c mice, divided into 10 groups: Basal, HDM, Laser (1J, 3J, 5J and 7,5J) and HDM+Laser (1J, 3J, 5J and 7,5J). The LLLT used was diode (660 nm, 100 mW) and for each energy, we used different times (10s, 30s, 50s and 75s) in three points: trachea, left lobe and right. For the experimental model, the animals received orotracheal administration of 100ug/animal HDM on days 0, 14, followed by 3 weekly administrations by day 56. After 24 hours, we studied functional and structural parameters, such as pulmonary inflammation through bronchoalveolar lavage fluid (BALF), total and differential cell count, quantification of CD11b, LY6G, Syglec F, CD3+, CD4+ and CD8+ in the lung, the percentage of 7AAD and ANEXINA V, as well as CD4+, STAT6+, GATA3+ and IL-4+ lymphocytes in BALF by flow cytometry, cytokines in BALF and pulmonary homogenate (IL-4, IL-5, IL-10 and IL-13) by ELISA, morphometric analysis of eosinophilic peribronchial infiltrate (LUNA), mucus (PAS), collagen (PSR), bronchoconstriction index, bronchial and muscular thickening (HE), pulmonary mechanics (pulmonary elastance) and pulmonary reactivity by the Power Lab method. Results showed that LLLT was able to modulate observed pulmonary inflammation by reducing the number of cells in the BALF, as well as reducing the percentage of neutrophils, eosinophils and T lymphocytes (CD4+ and CD8+). In addition, it decreased CD4+ STAT6+ and GATA3+ lymphocytes, as well as apoptosis and cell necrosis. On the other hand, laser therapy increased the percentage of macrophages and IL-10 and reduced levels of IL-4, IL-5 and IL-13 in the BALF and pulmonary homogenate. LLLT was able to reduce mucus production. Regarding the structural alterations, we observed a significant reduction in the quantification of peribronchial eosinophils, collagen deposition, bronchoconstriction index, bronchial and muscular 9 thickening in the airways, after LLLT treatment. Finally, in functional analysis, the evaluation of tracheal reactivity to methacholine and evaluation of pulmonary mechanics presented a decrease in all groups submitted to laser therapy. We conclude that the use of laser therapy in the treatment of chronic inflammation of the airways attenuated the inflammatory process, functional parameters and pulmonary structure. We emphasize in general that the 3J laser presented better results. Thus, photobiomodulation can be considered a promising tool for the treatment of chronic pulmonary allergic inflammation observed in asthma. |
publishDate |
2018 |
dc.date.issued.fl_str_mv |
2018-07-31 |
dc.date.accessioned.fl_str_mv |
2021-11-04T17:45:08Z |
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publishedVersion |
dc.identifier.citation.fl_str_mv |
Oliveira, Nicole Cristine Rigonato de. Efeito da terapia com Laser de Baixa Intensidade (LBI) em modelo experimental de inflamação alérgica pulmonar induzida por House Dust Mite (HDM) - estudo de dosimetria. 2018. 96 f. Tese( Programa de Pós-Graduação em Biofotônica Aplicada às Ciências da Saúde) - Universidade Nove de Julho, São Paulo. |
dc.identifier.uri.fl_str_mv |
http://bibliotecatede.uninove.br/handle/tede/2666 |
identifier_str_mv |
Oliveira, Nicole Cristine Rigonato de. Efeito da terapia com Laser de Baixa Intensidade (LBI) em modelo experimental de inflamação alérgica pulmonar induzida por House Dust Mite (HDM) - estudo de dosimetria. 2018. 96 f. Tese( Programa de Pós-Graduação em Biofotônica Aplicada às Ciências da Saúde) - Universidade Nove de Julho, São Paulo. |
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http://bibliotecatede.uninove.br/handle/tede/2666 |
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por |
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8765449414823306929 |
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600 |
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Universidade Nove de Julho |
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Programa de Pós-Graduação em Biofotônica Aplicada às Ciências da Saúde |
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UNINOVE |
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Brasil |
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Saúde |
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Universidade Nove de Julho |
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Biblioteca Digital de Teses e Dissertações da Uninove |
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