Efeito do laser de baixa intensidade sobre células musculares submetidas à ação do veneno de serpentes do gênero bothrops: mecanismo de morte celular
Ano de defesa: | 2018 |
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Autor(a) principal: | |
Orientador(a): | |
Banca de defesa: | , , |
Tipo de documento: | Dissertação |
Tipo de acesso: | Acesso aberto |
Idioma: | por |
Instituição de defesa: |
Universidade Nove de Julho
|
Programa de Pós-Graduação: |
Programa de Mestrado em Medicina
|
Departamento: |
Saúde
|
País: |
Brasil
|
Palavras-chave em Português: | |
Palavras-chave em Inglês: | |
Área do conhecimento CNPq: | |
Link de acesso: | http://bibliotecatede.uninove.br/handle/tede/2998 |
Resumo: | The envenoming caused by snakes of the Bothrops genus is considered a neglected tropical disease and is frequently associated with severe and complex local manifestations; such as edema, intense pain, hemorrhage and myonecrosis. The specific and conventional treatment for this condition is the use of the anti-venom serum, which neutralizes the majority of the circulating venom, thus minimizing its systemic effects. However, its local action is reduced and, therefore, the patient may present permanent tissue damage and, in some cases, even amputation of the affected member. Thus, therapies are sought that can complement the action of the serum and minimize the local effects of the venom. Previous studies in our group have demonstrated that the low level laser (LLL) increases the viability of C2C12 muscle cells incubated with the venom of Bothrops jararacussu and further promotes the differentiation of these cells. Thus, the objective of this work was to evaluate the effect of LLL on C2C12 muscle cells submitted to B. jararaca, B. jararacussu and B. moojeni venoms for their mechanism of cell death (necrosis and apoptosis). To do so, the cells were incubated with the respective venoms and immediately irradiated with LLL at the wavelength of 660 nm, energy density of 2.5 J/cm2, potency of 10 mW, area of 0.045 cm2 and time of application of 20 seconds. The cells were incubated for 2 hours and then flow cytometry was performed for analysis of cell death. Cell viability was also evaluated along with the dosage of inflammatory mediators such as interleukins IL-1 β, IL-6, IL-10 and Tumor Necrosis Factor α (TNF-α). Our results showed that the application of LLL decreases cell death in the different venoms by necrosis and apoptosis; increases cell viability; decreases the release of IL-6 to all venoms and IL-1 β for the venoms of B. jararaca and B. moojeni, but does not influence the release of IL-10 and TNF-α. Thus, the LLL protects the muscle cells against the action of the venoms studied; increasing cell viability by decreasing cell death (necrosis and apoptosis) and minimizing the inflammatory process. |
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Zamuner, Stella Reginahttp://lattes.cnpq.br/1935805744318404Zamuner, Stella Reginahttp://lattes.cnpq.br/1935805744318404Oliveira, Ana Paula Ligeiro dehttp://lattes.cnpq.br/5700855351581023Silva, Carlos Albertohttp://lattes.cnpq.br/5754147808501113http://lattes.cnpq.br/5915386069437932Gouveia, Viviane Almeida2022-07-21T19:32:32Z2018-06-05Gouveia, Viviane Almeida. Efeito do laser de baixa intensidade sobre células musculares submetidas à ação do veneno de serpentes do gênero bothrops: mecanismo de morte celular. 2018. 71 f. Dissertação( Programa de Mestrado em Medicina) - Universidade Nove de Julho, São Paulo.http://bibliotecatede.uninove.br/handle/tede/2998The envenoming caused by snakes of the Bothrops genus is considered a neglected tropical disease and is frequently associated with severe and complex local manifestations; such as edema, intense pain, hemorrhage and myonecrosis. The specific and conventional treatment for this condition is the use of the anti-venom serum, which neutralizes the majority of the circulating venom, thus minimizing its systemic effects. However, its local action is reduced and, therefore, the patient may present permanent tissue damage and, in some cases, even amputation of the affected member. Thus, therapies are sought that can complement the action of the serum and minimize the local effects of the venom. Previous studies in our group have demonstrated that the low level laser (LLL) increases the viability of C2C12 muscle cells incubated with the venom of Bothrops jararacussu and further promotes the differentiation of these cells. Thus, the objective of this work was to evaluate the effect of LLL on C2C12 muscle cells submitted to B. jararaca, B. jararacussu and B. moojeni venoms for their mechanism of cell death (necrosis and apoptosis). To do so, the cells were incubated with the respective venoms and immediately irradiated with LLL at the wavelength of 660 nm, energy density of 2.5 J/cm2, potency of 10 mW, area of 0.045 cm2 and time of application of 20 seconds. The cells were incubated for 2 hours and then flow cytometry was performed for analysis of cell death. Cell viability was also evaluated along with the dosage of inflammatory mediators such as interleukins IL-1 β, IL-6, IL-10 and Tumor Necrosis Factor α (TNF-α). Our results showed that the application of LLL decreases cell death in the different venoms by necrosis and apoptosis; increases cell viability; decreases the release of IL-6 to all venoms and IL-1 β for the venoms of B. jararaca and B. moojeni, but does not influence the release of IL-10 and TNF-α. Thus, the LLL protects the muscle cells against the action of the venoms studied; increasing cell viability by decreasing cell death (necrosis and apoptosis) and minimizing the inflammatory process.O envenenamento causado por serpentes do gênero Bothrops é considerado uma doença tropical negligenciada e frequentemente está associado com manifestações locais graves e complexas; como edema, dor intensa, hemorragia e mionecrose. O tratamento convencional específico para esse agravo é a utilização do soro antibotrópico, o qual neutraliza grande parte do veneno circulante, minimizando assim seus efeitos sistêmicos. Porém, sua ação local é reduzida e, com isso, o paciente pode apresentar lesão permanente de tecidos e, em alguns casos, até mesmo a amputação do membro afetado. Assim, buscam-se terapias que possam complementar a ação do soro e minimizar os efeitos locais do veneno. Estudos anteriores do nosso grupo demonstraram que o laser de baixa intensidade (LBI) aumenta a viabilidade de células musculares C2C12 incubadas com o veneno de Bothrops jararacussu e ainda promove a diferenciação dessas células. Dessa maneira, o objetivo deste trabalho foi avaliar o efeito do LBI em células musculares C2C12 submetidas à ação dos venenos de B. jararaca, B. jararacussu e B. moojeni quanto ao seu mecanismo de morte celular (necrose e apoptose). Para tanto, as células foram incubadas com os respectivos venenos e imediatamente irradiadas com LBI no comprimento de onda de 660 nm, densidade de energia de 2,5 J/cm2, potência de 10 mW, área de 0,045 cm2 e tempo de aplicação de 20 segundos. As células foram incubadas por 2 horas e, posteriormente, foi realizada a citometria de fluxo para análise da morte celular. Também foi avaliada a viabilidade celular juntamente com a dosagem de mediadores inflamatórios como as interleucinas IL-1 β, IL-6, IL-10 e Fator de Necrose Tumoral α (TNF-α). Nossos resultados mostraram que a aplicação do LBI diminui a morte celular nos diferentes venenos por necrose e apoptose; aumenta a viabilidade celular; diminui a liberação de IL-6 para todos os venenos e de IL-1 β para os venenos de B. jararaca e B. moojeni, mas não influencia na liberação de IL-10 e TNF-α. Assim, o LBI protege as células musculares contra a ação dos venenos estudados; aumentando a viabilidade celular pela diminuição da morte celular (necrose e apoptose) e minimizando o processo inflamatório.Submitted by Nadir Basilio (nadirsb@uninove.br) on 2022-07-21T19:32:32Z No. of bitstreams: 1 Viviane Almeida Gouveia.pdf: 2295666 bytes, checksum: 3f1065693ee4138e68ad850e856b5f8b (MD5)Made available in DSpace on 2022-07-21T19:32:32Z (GMT). No. of bitstreams: 1 Viviane Almeida Gouveia.pdf: 2295666 bytes, checksum: 3f1065693ee4138e68ad850e856b5f8b (MD5) Previous issue date: 2018-06-05application/pdfporUniversidade Nove de JulhoPrograma de Mestrado em MedicinaUNINOVEBrasilSaúdecélulas musculareslaser de baixa intensidadeapoptosenecroseB. jararacaB. jararacussuB. moojenimuscle cellslow level laserapoptosisnecrosisB. jararacaB. jararacussuB. moojeniCIENCIAS DA SAUDEEfeito do laser de baixa intensidade sobre células musculares submetidas à ação do veneno de serpentes do gênero bothrops: mecanismo de morte celularEffect of low level laser on muscle cells submitted to the action of snake venom of the genes bothrops: cell death mechanisminfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesis8765449414823306929600info:eu-repo/semantics/openAccessreponame:Biblioteca Digital de Teses e Dissertações da Uninoveinstname:Universidade Nove de Julho (UNINOVE)instacron:UNINOVEORIGINALViviane Almeida Gouveia.pdfViviane Almeida Gouveia.pdfapplication/pdf2295666http://localhost:8080/tede/bitstream/tede/2998/2/Viviane+Almeida+Gouveia.pdf3f1065693ee4138e68ad850e856b5f8bMD52LICENSElicense.txtlicense.txttext/plain; charset=utf-82165http://localhost:8080/tede/bitstream/tede/2998/1/license.txtbd3efa91386c1718a7f26a329fdcb468MD51tede/29982022-07-21 16:32:32.8oai:localhost: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Biblioteca Digital de Teses e Dissertaçõeshttp://bibliotecatede.uninove.br/PRIhttp://bibliotecatede.uninove.br/oai/requestbibliotecatede@uninove.br||bibliotecatede@uninove.bropendoar:2022-07-21T19:32:32Biblioteca Digital de Teses e Dissertações da Uninove - Universidade Nove de Julho (UNINOVE)false |
dc.title.por.fl_str_mv |
Efeito do laser de baixa intensidade sobre células musculares submetidas à ação do veneno de serpentes do gênero bothrops: mecanismo de morte celular |
dc.title.alternative.eng.fl_str_mv |
Effect of low level laser on muscle cells submitted to the action of snake venom of the genes bothrops: cell death mechanism |
title |
Efeito do laser de baixa intensidade sobre células musculares submetidas à ação do veneno de serpentes do gênero bothrops: mecanismo de morte celular |
spellingShingle |
Efeito do laser de baixa intensidade sobre células musculares submetidas à ação do veneno de serpentes do gênero bothrops: mecanismo de morte celular Gouveia, Viviane Almeida células musculares laser de baixa intensidade apoptose necrose B. jararaca B. jararacussu B. moojeni muscle cells low level laser apoptosis necrosis B. jararaca B. jararacussu B. moojeni CIENCIAS DA SAUDE |
title_short |
Efeito do laser de baixa intensidade sobre células musculares submetidas à ação do veneno de serpentes do gênero bothrops: mecanismo de morte celular |
title_full |
Efeito do laser de baixa intensidade sobre células musculares submetidas à ação do veneno de serpentes do gênero bothrops: mecanismo de morte celular |
title_fullStr |
Efeito do laser de baixa intensidade sobre células musculares submetidas à ação do veneno de serpentes do gênero bothrops: mecanismo de morte celular |
title_full_unstemmed |
Efeito do laser de baixa intensidade sobre células musculares submetidas à ação do veneno de serpentes do gênero bothrops: mecanismo de morte celular |
title_sort |
Efeito do laser de baixa intensidade sobre células musculares submetidas à ação do veneno de serpentes do gênero bothrops: mecanismo de morte celular |
author |
Gouveia, Viviane Almeida |
author_facet |
Gouveia, Viviane Almeida |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Zamuner, Stella Regina |
dc.contributor.advisor1Lattes.fl_str_mv |
http://lattes.cnpq.br/1935805744318404 |
dc.contributor.referee1.fl_str_mv |
Zamuner, Stella Regina |
dc.contributor.referee1Lattes.fl_str_mv |
http://lattes.cnpq.br/1935805744318404 |
dc.contributor.referee2.fl_str_mv |
Oliveira, Ana Paula Ligeiro de |
dc.contributor.referee2Lattes.fl_str_mv |
http://lattes.cnpq.br/5700855351581023 |
dc.contributor.referee3.fl_str_mv |
Silva, Carlos Alberto |
dc.contributor.referee3Lattes.fl_str_mv |
http://lattes.cnpq.br/5754147808501113 |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/5915386069437932 |
dc.contributor.author.fl_str_mv |
Gouveia, Viviane Almeida |
contributor_str_mv |
Zamuner, Stella Regina Zamuner, Stella Regina Oliveira, Ana Paula Ligeiro de Silva, Carlos Alberto |
dc.subject.por.fl_str_mv |
células musculares laser de baixa intensidade apoptose necrose B. jararaca B. jararacussu B. moojeni |
topic |
células musculares laser de baixa intensidade apoptose necrose B. jararaca B. jararacussu B. moojeni muscle cells low level laser apoptosis necrosis B. jararaca B. jararacussu B. moojeni CIENCIAS DA SAUDE |
dc.subject.eng.fl_str_mv |
muscle cells low level laser apoptosis necrosis B. jararaca B. jararacussu B. moojeni |
dc.subject.cnpq.fl_str_mv |
CIENCIAS DA SAUDE |
description |
The envenoming caused by snakes of the Bothrops genus is considered a neglected tropical disease and is frequently associated with severe and complex local manifestations; such as edema, intense pain, hemorrhage and myonecrosis. The specific and conventional treatment for this condition is the use of the anti-venom serum, which neutralizes the majority of the circulating venom, thus minimizing its systemic effects. However, its local action is reduced and, therefore, the patient may present permanent tissue damage and, in some cases, even amputation of the affected member. Thus, therapies are sought that can complement the action of the serum and minimize the local effects of the venom. Previous studies in our group have demonstrated that the low level laser (LLL) increases the viability of C2C12 muscle cells incubated with the venom of Bothrops jararacussu and further promotes the differentiation of these cells. Thus, the objective of this work was to evaluate the effect of LLL on C2C12 muscle cells submitted to B. jararaca, B. jararacussu and B. moojeni venoms for their mechanism of cell death (necrosis and apoptosis). To do so, the cells were incubated with the respective venoms and immediately irradiated with LLL at the wavelength of 660 nm, energy density of 2.5 J/cm2, potency of 10 mW, area of 0.045 cm2 and time of application of 20 seconds. The cells were incubated for 2 hours and then flow cytometry was performed for analysis of cell death. Cell viability was also evaluated along with the dosage of inflammatory mediators such as interleukins IL-1 β, IL-6, IL-10 and Tumor Necrosis Factor α (TNF-α). Our results showed that the application of LLL decreases cell death in the different venoms by necrosis and apoptosis; increases cell viability; decreases the release of IL-6 to all venoms and IL-1 β for the venoms of B. jararaca and B. moojeni, but does not influence the release of IL-10 and TNF-α. Thus, the LLL protects the muscle cells against the action of the venoms studied; increasing cell viability by decreasing cell death (necrosis and apoptosis) and minimizing the inflammatory process. |
publishDate |
2018 |
dc.date.issued.fl_str_mv |
2018-06-05 |
dc.date.accessioned.fl_str_mv |
2022-07-21T19:32:32Z |
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publishedVersion |
dc.identifier.citation.fl_str_mv |
Gouveia, Viviane Almeida. Efeito do laser de baixa intensidade sobre células musculares submetidas à ação do veneno de serpentes do gênero bothrops: mecanismo de morte celular. 2018. 71 f. Dissertação( Programa de Mestrado em Medicina) - Universidade Nove de Julho, São Paulo. |
dc.identifier.uri.fl_str_mv |
http://bibliotecatede.uninove.br/handle/tede/2998 |
identifier_str_mv |
Gouveia, Viviane Almeida. Efeito do laser de baixa intensidade sobre células musculares submetidas à ação do veneno de serpentes do gênero bothrops: mecanismo de morte celular. 2018. 71 f. Dissertação( Programa de Mestrado em Medicina) - Universidade Nove de Julho, São Paulo. |
url |
http://bibliotecatede.uninove.br/handle/tede/2998 |
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UNINOVE |
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Brasil |
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Universidade Nove de Julho |
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