Efeito protetor do laser de baixa pot??ncia em diferentes momentos sobre o processo de reparo do m??sculo esquel??tico de ratos
Ano de defesa: | 2017 |
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Autor(a) principal: | |
Orientador(a): | |
Banca de defesa: | , , , , |
Tipo de documento: | Tese |
Tipo de acesso: | Acesso aberto |
Idioma: | por |
Instituição de defesa: |
Universidade Nove de Julho
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Programa de Pós-Graduação: |
Programa de P??s-Gradua????o em Ci??ncias da Reabilita????o
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Departamento: |
Sa??de
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País: |
Brasil
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Palavras-chave em Português: | |
Palavras-chave em Inglês: | |
Área do conhecimento CNPq: | |
Link de acesso: | http://bibliotecatede.uninove.br/handle/tede/1835 |
Resumo: | The low level laser therapy (LLLT) is a resource capable of modulating in a positive way the different stages of the muscle repair process. However, little has been described about its effects when applied prior to injury, associated or not with post-injury treatment. The general objective of the present study was to evaluate the effect of LLLT applied prior to the muscle injury associated or not to the post-injury application on the rat skeletal muscle repair process. The aim of article 1 was to evaluate the effect of infrared LLLT applied prior to cryoinjury on the expression of IL-6, MyoD and myogenin. The aim of article 2 was to evaluate the effect of red and infrared LLLT prior to cryoinjury associated or not with post-injury application on oxidative stress. The aim of article 3 was to evaluate the effect of infrared LLLT applied prior to injury with or without post-injury application on the gene expression and protein synthesis of inflammatory cytokines TNF-?? and IL-6, and muscle morphology. For the three studies, 205 Wistar rats were divided into the following experimental groups: Control; Injury; LLLT 660 nm prior to injury; LLLT 780 nm prior to injury; LLLT 660 nm pre- and post-injury; and LLLT 780 nm pre- and post-injury. The cryoinjury consisted of two applications of cane cooled in liquid nitrogen in the anterior tibial muscle (TA). The injured groups were evaluated at 1, 3, 7 and 14 days after injury (only for oxidative stress analysis, evaluation occurred at 1, 3, and 7 days). LLLT irradiation was performed with the AsGaAl laser (780 nm) at the parameters of 10 J/cm??; 40 mW; 10 seconds per point; 8 points; 3.2 J. At the end of the protocol, TA muscles were carefully removed for analysis of oxidative stress by lipid peroxidation (LPO) by chemiluminescence initiated by t-BOOH, protein oxidation through the carbonyl damage assay, and analysis of antioxidant enzymes: catalase (CAT), superoxide dismutase (SOD) and glutathione peroxidase (GPx). TNF-?? and IL-6 cytokines were analyzed by quantitative real-time PCR for the detection of gene expression. In addition, a morphological analysis was performed by staining with hematoxylin and eosin. TNF-?? and IL-6 cytokines were analyzed by ELISA for protein expression. Data were expressed as mean and standard error of the mean because they were parametric and the comparison between the groups was performed by ANOVA/Tukey (p<0.05). The results of article 1 demonstrate a reduction in the levels of IL-6 mRNA in the group irradiated prior to the injury, concluding that the LBP applied prior to injury was able to modulate positively this inflammatory cytokine, but had no effect on the myogenic regulatory factors MyoD and myogenin in the evaluated parameters. The results of article 2 containing the oxidative stress analysis showed that the irradiated groups presented reduction of the lipoperoxidation and positive modulation of the antioxidant enzymes during the muscle repair process, concluding the red and infrared laser were able to modulate positively the oxidative stress, being the most effective infrared laser in the modulation of antioxidant enzymes. The results of article 3 demonstrated a reduction in the gene expression of TNF-?? in the group irradiated prior to injury. In the pre-and post-injury irradiated group, IL-6 and TNF-?? mRNA levels increased during the skeletal muscle repair process, concluding that the infrared laser was able to positive modulation these inflammatory cytokines in the two forms of application. Therefore, it is concluded that the LLLT irradiated prior to injury associated or not with the post-injury application has a beneficial effect on inflammatory cytokines and oxidative stress during the repair of the skeletal muscle of rats. |
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Ferrari, Raquel Agnelli MesquitaFerrari, Raquel Agnelli MesquitaBussadori, Sandra KalilJorge, Luciana Maria Malos?? SampaioBossini, Paulo SergioPinfild, Carlos Eduardohttp://lattes.cnpq.br/2211419015181709Ribeiro, Beatriz Guimar??es2018-07-16T20:01:20Z2017-02-23Ribeiro, Beatriz Guimar??es. Efeito protetor do laser de baixa pot??ncia em diferentes momentos sobre o processo de reparo do m??sculo esquel??tico de ratos. 2017. 78 f. Tese( Programa de P??s-Gradua????o em Ci??ncias da Reabilita????o) - Universidade Nove de Julho, S??o Paulo.http://bibliotecatede.uninove.br/handle/tede/1835The low level laser therapy (LLLT) is a resource capable of modulating in a positive way the different stages of the muscle repair process. However, little has been described about its effects when applied prior to injury, associated or not with post-injury treatment. The general objective of the present study was to evaluate the effect of LLLT applied prior to the muscle injury associated or not to the post-injury application on the rat skeletal muscle repair process. The aim of article 1 was to evaluate the effect of infrared LLLT applied prior to cryoinjury on the expression of IL-6, MyoD and myogenin. The aim of article 2 was to evaluate the effect of red and infrared LLLT prior to cryoinjury associated or not with post-injury application on oxidative stress. The aim of article 3 was to evaluate the effect of infrared LLLT applied prior to injury with or without post-injury application on the gene expression and protein synthesis of inflammatory cytokines TNF-?? and IL-6, and muscle morphology. For the three studies, 205 Wistar rats were divided into the following experimental groups: Control; Injury; LLLT 660 nm prior to injury; LLLT 780 nm prior to injury; LLLT 660 nm pre- and post-injury; and LLLT 780 nm pre- and post-injury. The cryoinjury consisted of two applications of cane cooled in liquid nitrogen in the anterior tibial muscle (TA). The injured groups were evaluated at 1, 3, 7 and 14 days after injury (only for oxidative stress analysis, evaluation occurred at 1, 3, and 7 days). LLLT irradiation was performed with the AsGaAl laser (780 nm) at the parameters of 10 J/cm??; 40 mW; 10 seconds per point; 8 points; 3.2 J. At the end of the protocol, TA muscles were carefully removed for analysis of oxidative stress by lipid peroxidation (LPO) by chemiluminescence initiated by t-BOOH, protein oxidation through the carbonyl damage assay, and analysis of antioxidant enzymes: catalase (CAT), superoxide dismutase (SOD) and glutathione peroxidase (GPx). TNF-?? and IL-6 cytokines were analyzed by quantitative real-time PCR for the detection of gene expression. In addition, a morphological analysis was performed by staining with hematoxylin and eosin. TNF-?? and IL-6 cytokines were analyzed by ELISA for protein expression. Data were expressed as mean and standard error of the mean because they were parametric and the comparison between the groups was performed by ANOVA/Tukey (p<0.05). The results of article 1 demonstrate a reduction in the levels of IL-6 mRNA in the group irradiated prior to the injury, concluding that the LBP applied prior to injury was able to modulate positively this inflammatory cytokine, but had no effect on the myogenic regulatory factors MyoD and myogenin in the evaluated parameters. The results of article 2 containing the oxidative stress analysis showed that the irradiated groups presented reduction of the lipoperoxidation and positive modulation of the antioxidant enzymes during the muscle repair process, concluding the red and infrared laser were able to modulate positively the oxidative stress, being the most effective infrared laser in the modulation of antioxidant enzymes. The results of article 3 demonstrated a reduction in the gene expression of TNF-?? in the group irradiated prior to injury. In the pre-and post-injury irradiated group, IL-6 and TNF-?? mRNA levels increased during the skeletal muscle repair process, concluding that the infrared laser was able to positive modulation these inflammatory cytokines in the two forms of application. Therefore, it is concluded that the LLLT irradiated prior to injury associated or not with the post-injury application has a beneficial effect on inflammatory cytokines and oxidative stress during the repair of the skeletal muscle of rats.O laser de baixa pot??ncia (LBP) ?? um recurso capaz de modular de forma positiva as diferentes etapas do processo de reparo muscular. Contudo, pouco h?? descrito sobre seus efeitos quando aplicado previamente a uma les??o, associado ou n??o a um tratamento p??s-les??o. O objetivo geral do presente estudo foi avaliar o efeito do LBP aplicado previamente ?? les??o muscular associado ou n??o a aplica????o p??s-les??o sobre o processo de reparo musculo esquel??tico de rato. O objetivo do artigo 1 foi avaliar o efeito do LBP infravermelho aplicado previamente ?? crioles??o sobre a express??o de IL-6, MyoD e miogenina. O objetivo do artigo 2 foi avaliar o efeito do LBP vermelho e infravermelho previamente a crioles??o associado ou n??o a aplica????o p??s-les??o sobre o estresse oxidativo. O objetivo do artigo 3 foi avaliar o efeito do LBP infravermelho aplicado previamente ?? crioles??o associado ou n??o a aplica????o p??s-les??o sobre a express??o g??nica e s??ntese proteica das citocinas inflamat??rias TNF-?? e IL-6, e morfologia muscular. Para a realiza????o dos tr??s estudos, foram utilizados 205 ratos Wistar, divididos nos seguintes grupos experimentais: Controle; Somente les??o; LBP 660 nm previamente ?? les??o; LBP 780 nm previamente ?? les??o; LBP 660 nm pr?? e p??s-les??o; e LBP 780 nm pr?? e p??s-les??o. A crioles??o consistiu de duas aplica????es de bast??o resfriado em nitrog??nio l??quido no m??sculo tibial anterior (TA). Os grupos lesionados foram avaliados em 1, 3, 7 e 14 dias ap??s a les??o (somente para an??lise de estresse oxidativo, a avalia????o ocorreu em 1, 3, e 7 dias). A irradia????o com LBP foi realizada com o laser AsGaAl (780 nm) nos par??metros de 10 J/cm??; 40 mW; 10 segundos por ponto; 8 pontos; 3.2 J. Ao t??rmino do protocolo, os m??sculos TA foram retirados cuidadosamente para an??lise de estresse oxidativo pela lipoperoxida????o (LPO) por quimiluminesc??ncia iniciada por t-BOOH, pela oxida????o proteica atrav??s do ensaio de dano as carbonilas, e pela an??lise das enzimas antioxidantes: catalase (CAT), super??xido dismutase (SOD) e glutationa peroxidase (GPx). Tamb??m foram analisadas as citocinas TNF-?? e IL-6 por PCR em tempo real quantitativo para detec????o da express??o g??nica. Al??m disso, foi realizada an??lise morfol??gica por colora????o com hematoxilina e eosina. As citocinas TNF-?? e IL-6 tamb??m foram analisadas por ELISA para detec????o da express??o proteica. Os dados foram expressos em m??dia e erro-padr??o da m??dia por se apresentarem param??tricos e a compara????o entre os grupos foi realizada pelo ANOVA/ Tukey (p<0,05). Os resultados do artigo 1 demonstram redu????o nos n??veis de mRNA IL-6 no grupo irradiado previamente ?? les??o, concluindo que o LBP aplicado previamente ?? les??o foi capaz de modular positivamente essa citocina inflamat??ria, mas n??o teve efeito sobre os fatores regulat??rios miog??nicos MyoD e miogenina nos par??metros avaliados. Os resultados do artigo 2 contendo a an??lise de estresse oxidativo demonstram que os grupos irradiados apresentaram redu????o da lipoperoxida????o e modula????o positiva das enzimas antioxidantes durante o processo de reparo muscular, concluindo que tanto o laser vermelho como infravermelho foram capazes de modular positivamente o estresse oxidativo sendo o laser infravermelho mais eficaz na modula????o de enzimas antioxidantes. Os resultados do artigo 3 demonstram uma redu????o na express??o g??nica de TNF-?? no grupo irradiado previamente a les??o. J?? no grupo irradiado pr?? e p??s-les??o, houve aumento dos n??veis de mRNA IL-6 e TNF-?? durante o processo de reparo musculo esquel??tico, concluindo que o laser infravermelho foi capaz de modular de forma positiva essas citocinas inflamat??rias nas duas formas de aplica????o. Portanto, conclui-se de forma geral que o LBP utilizado previamente ?? les??o associado ou n??o a aplica????o p??s-les??o tem efeito ben??fico sobre a modula????o de citocinas inflamat??rias e do estresse oxidativo durante o reparo do m??sculo esquel??tico de ratos.Submitted by Nadir Basilio (nadirsb@uninove.br) on 2018-07-16T20:01:20Z No. of bitstreams: 1 Beatriz Guimar??es Ribeiro.pdf: 1467862 bytes, checksum: d166e20babcfe778cdc4c7cf5708fc53 (MD5)Made available in DSpace on 2018-07-16T20:01:20Z (GMT). No. of bitstreams: 1 Beatriz Guimar??es Ribeiro.pdf: 1467862 bytes, checksum: d166e20babcfe778cdc4c7cf5708fc53 (MD5) Previous issue date: 2017-02-23application/pdfporUniversidade Nove de JulhoPrograma de P??s-Gradua????o em Ci??ncias da Reabilita????oUNINOVEBrasilSa??deterapia a laser de baixa pot??nciafotobiomodula????om??sculo tibial anteriorregenera????oestresse oxidativocitocinasfototerapialow-level light therapyphotobiomodulationskeletal muscleregenerationoxidative stresscytokinesphototherapyCIENCIAS DA SAUDEEfeito protetor do laser de baixa pot??ncia em diferentes momentos sobre o processo de reparo do m??sculo esquel??tico de ratosProtective effect of the low level laser therapy at different moments on the muscle repair process in ratsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/doctoralThesis8765449414823306929600info:eu-repo/semantics/openAccessreponame:Biblioteca Digital de Teses e Dissertações da Uninoveinstname:Universidade Nove de Julho (UNINOVE)instacron:UNINOVEORIGINALBeatriz Guimar??es Ribeiro.pdfBeatriz Guimar??es Ribeiro.pdfapplication/pdf1467862http://localhost:8080/tede/bitstream/tede/1835/2/Beatriz+Guimar%C3%A3es+Ribeiro.pdfd166e20babcfe778cdc4c7cf5708fc53MD52LICENSElicense.txtlicense.txttext/plain; charset=utf-82165http://localhost:8080/tede/bitstream/tede/1835/1/license.txtbd3efa91386c1718a7f26a329fdcb468MD51tede/18352018-07-16 17:01:20.921oai:localhost: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Biblioteca Digital de Teses e Dissertaçõeshttp://bibliotecatede.uninove.br/PRIhttp://bibliotecatede.uninove.br/oai/requestbibliotecatede@uninove.br||bibliotecatede@uninove.bropendoar:2018-07-16T20:01:20Biblioteca Digital de Teses e Dissertações da Uninove - Universidade Nove de Julho (UNINOVE)false |
dc.title.por.fl_str_mv |
Efeito protetor do laser de baixa pot??ncia em diferentes momentos sobre o processo de reparo do m??sculo esquel??tico de ratos |
dc.title.alternative.eng.fl_str_mv |
Protective effect of the low level laser therapy at different moments on the muscle repair process in rats |
title |
Efeito protetor do laser de baixa pot??ncia em diferentes momentos sobre o processo de reparo do m??sculo esquel??tico de ratos |
spellingShingle |
Efeito protetor do laser de baixa pot??ncia em diferentes momentos sobre o processo de reparo do m??sculo esquel??tico de ratos Ribeiro, Beatriz Guimar??es terapia a laser de baixa pot??ncia fotobiomodula????o m??sculo tibial anterior regenera????o estresse oxidativo citocinas fototerapia low-level light therapy photobiomodulation skeletal muscle regeneration oxidative stress cytokines phototherapy CIENCIAS DA SAUDE |
title_short |
Efeito protetor do laser de baixa pot??ncia em diferentes momentos sobre o processo de reparo do m??sculo esquel??tico de ratos |
title_full |
Efeito protetor do laser de baixa pot??ncia em diferentes momentos sobre o processo de reparo do m??sculo esquel??tico de ratos |
title_fullStr |
Efeito protetor do laser de baixa pot??ncia em diferentes momentos sobre o processo de reparo do m??sculo esquel??tico de ratos |
title_full_unstemmed |
Efeito protetor do laser de baixa pot??ncia em diferentes momentos sobre o processo de reparo do m??sculo esquel??tico de ratos |
title_sort |
Efeito protetor do laser de baixa pot??ncia em diferentes momentos sobre o processo de reparo do m??sculo esquel??tico de ratos |
author |
Ribeiro, Beatriz Guimar??es |
author_facet |
Ribeiro, Beatriz Guimar??es |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Ferrari, Raquel Agnelli Mesquita |
dc.contributor.referee1.fl_str_mv |
Ferrari, Raquel Agnelli Mesquita |
dc.contributor.referee2.fl_str_mv |
Bussadori, Sandra Kalil |
dc.contributor.referee3.fl_str_mv |
Jorge, Luciana Maria Malos?? Sampaio |
dc.contributor.referee4.fl_str_mv |
Bossini, Paulo Sergio |
dc.contributor.referee5.fl_str_mv |
Pinfild, Carlos Eduardo |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/2211419015181709 |
dc.contributor.author.fl_str_mv |
Ribeiro, Beatriz Guimar??es |
contributor_str_mv |
Ferrari, Raquel Agnelli Mesquita Ferrari, Raquel Agnelli Mesquita Bussadori, Sandra Kalil Jorge, Luciana Maria Malos?? Sampaio Bossini, Paulo Sergio Pinfild, Carlos Eduardo |
dc.subject.por.fl_str_mv |
terapia a laser de baixa pot??ncia fotobiomodula????o m??sculo tibial anterior regenera????o estresse oxidativo citocinas fototerapia |
topic |
terapia a laser de baixa pot??ncia fotobiomodula????o m??sculo tibial anterior regenera????o estresse oxidativo citocinas fototerapia low-level light therapy photobiomodulation skeletal muscle regeneration oxidative stress cytokines phototherapy CIENCIAS DA SAUDE |
dc.subject.eng.fl_str_mv |
low-level light therapy photobiomodulation skeletal muscle regeneration oxidative stress cytokines phototherapy |
dc.subject.cnpq.fl_str_mv |
CIENCIAS DA SAUDE |
description |
The low level laser therapy (LLLT) is a resource capable of modulating in a positive way the different stages of the muscle repair process. However, little has been described about its effects when applied prior to injury, associated or not with post-injury treatment. The general objective of the present study was to evaluate the effect of LLLT applied prior to the muscle injury associated or not to the post-injury application on the rat skeletal muscle repair process. The aim of article 1 was to evaluate the effect of infrared LLLT applied prior to cryoinjury on the expression of IL-6, MyoD and myogenin. The aim of article 2 was to evaluate the effect of red and infrared LLLT prior to cryoinjury associated or not with post-injury application on oxidative stress. The aim of article 3 was to evaluate the effect of infrared LLLT applied prior to injury with or without post-injury application on the gene expression and protein synthesis of inflammatory cytokines TNF-?? and IL-6, and muscle morphology. For the three studies, 205 Wistar rats were divided into the following experimental groups: Control; Injury; LLLT 660 nm prior to injury; LLLT 780 nm prior to injury; LLLT 660 nm pre- and post-injury; and LLLT 780 nm pre- and post-injury. The cryoinjury consisted of two applications of cane cooled in liquid nitrogen in the anterior tibial muscle (TA). The injured groups were evaluated at 1, 3, 7 and 14 days after injury (only for oxidative stress analysis, evaluation occurred at 1, 3, and 7 days). LLLT irradiation was performed with the AsGaAl laser (780 nm) at the parameters of 10 J/cm??; 40 mW; 10 seconds per point; 8 points; 3.2 J. At the end of the protocol, TA muscles were carefully removed for analysis of oxidative stress by lipid peroxidation (LPO) by chemiluminescence initiated by t-BOOH, protein oxidation through the carbonyl damage assay, and analysis of antioxidant enzymes: catalase (CAT), superoxide dismutase (SOD) and glutathione peroxidase (GPx). TNF-?? and IL-6 cytokines were analyzed by quantitative real-time PCR for the detection of gene expression. In addition, a morphological analysis was performed by staining with hematoxylin and eosin. TNF-?? and IL-6 cytokines were analyzed by ELISA for protein expression. Data were expressed as mean and standard error of the mean because they were parametric and the comparison between the groups was performed by ANOVA/Tukey (p<0.05). The results of article 1 demonstrate a reduction in the levels of IL-6 mRNA in the group irradiated prior to the injury, concluding that the LBP applied prior to injury was able to modulate positively this inflammatory cytokine, but had no effect on the myogenic regulatory factors MyoD and myogenin in the evaluated parameters. The results of article 2 containing the oxidative stress analysis showed that the irradiated groups presented reduction of the lipoperoxidation and positive modulation of the antioxidant enzymes during the muscle repair process, concluding the red and infrared laser were able to modulate positively the oxidative stress, being the most effective infrared laser in the modulation of antioxidant enzymes. The results of article 3 demonstrated a reduction in the gene expression of TNF-?? in the group irradiated prior to injury. In the pre-and post-injury irradiated group, IL-6 and TNF-?? mRNA levels increased during the skeletal muscle repair process, concluding that the infrared laser was able to positive modulation these inflammatory cytokines in the two forms of application. Therefore, it is concluded that the LLLT irradiated prior to injury associated or not with the post-injury application has a beneficial effect on inflammatory cytokines and oxidative stress during the repair of the skeletal muscle of rats. |
publishDate |
2017 |
dc.date.issued.fl_str_mv |
2017-02-23 |
dc.date.accessioned.fl_str_mv |
2018-07-16T20:01:20Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/doctoralThesis |
format |
doctoralThesis |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
Ribeiro, Beatriz Guimar??es. Efeito protetor do laser de baixa pot??ncia em diferentes momentos sobre o processo de reparo do m??sculo esquel??tico de ratos. 2017. 78 f. Tese( Programa de P??s-Gradua????o em Ci??ncias da Reabilita????o) - Universidade Nove de Julho, S??o Paulo. |
dc.identifier.uri.fl_str_mv |
http://bibliotecatede.uninove.br/handle/tede/1835 |
identifier_str_mv |
Ribeiro, Beatriz Guimar??es. Efeito protetor do laser de baixa pot??ncia em diferentes momentos sobre o processo de reparo do m??sculo esquel??tico de ratos. 2017. 78 f. Tese( Programa de P??s-Gradua????o em Ci??ncias da Reabilita????o) - Universidade Nove de Julho, S??o Paulo. |
url |
http://bibliotecatede.uninove.br/handle/tede/1835 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.cnpq.fl_str_mv |
8765449414823306929 |
dc.relation.confidence.fl_str_mv |
600 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Nove de Julho |
dc.publisher.program.fl_str_mv |
Programa de P??s-Gradua????o em Ci??ncias da Reabilita????o |
dc.publisher.initials.fl_str_mv |
UNINOVE |
dc.publisher.country.fl_str_mv |
Brasil |
dc.publisher.department.fl_str_mv |
Sa??de |
publisher.none.fl_str_mv |
Universidade Nove de Julho |
dc.source.none.fl_str_mv |
reponame:Biblioteca Digital de Teses e Dissertações da Uninove instname:Universidade Nove de Julho (UNINOVE) instacron:UNINOVE |
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Universidade Nove de Julho (UNINOVE) |
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UNINOVE |
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UNINOVE |
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Biblioteca Digital de Teses e Dissertações da Uninove |
collection |
Biblioteca Digital de Teses e Dissertações da Uninove |
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Biblioteca Digital de Teses e Dissertações da Uninove - Universidade Nove de Julho (UNINOVE) |
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bibliotecatede@uninove.br||bibliotecatede@uninove.br |
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