Caracteriza????o dos pept??deos indicadores de degrada????o do col??geno, N-acetyl-PGP e PGP, na saliva e urina de pacientes com Doen??a Pulmonar Obstrutiva Cr??nica
Ano de defesa: | 2011 |
---|---|
Autor(a) principal: | |
Orientador(a): | |
Banca de defesa: | , |
Tipo de documento: | Dissertação |
Tipo de acesso: | Acesso aberto |
Idioma: | por |
Instituição de defesa: |
Universidade Nove de Julho
|
Programa de Pós-Graduação: |
Programa de P??s-Gradua????o em Ci??ncias da Reabilita????o
|
Departamento: |
Sa??de
|
País: |
BR
|
Palavras-chave em Português: | |
Palavras-chave em Inglês: | |
Área do conhecimento CNPq: | |
Link de acesso: | http://bibliotecatede.uninove.br/tede/handle/tede/840 |
Resumo: | The Chronic Obstructive Pulmonary Disease (COPD) is characterized by a chronic limitation of the airflow and an abnormal inflammatory lung response to particles or harmful gases. The COPD is associated with an intense degradation of the extracellular matrix components (ECM) such as the Collagen fibers and Elastin, caused by the imbalance of enzymes in the parenchymal pulmonary. Reports show that the Collagen Hydrolysis produce peptides that are biologically actives (N-acetyl-PGP and PGP) that induces the neutrophil chemo-taxes through the interaction among the receptors CXCR1 and CXCR2 in the bronchoalveolar lavage fluid, and in the blood of the patients with COPD. Objective: To analyze the presence of the N-acetyl-PGP and PGP peptides as specifics indicators of the collagen degradation on the patients with COPD versus the healthy individuals. Methods: The Experiment was done with 25 individuals, grouped under patients with COPD (n=16) and healthy individuals (n=9). After the initial procedures for this experiment, all participants were submitted to the Spectrometric Test. The six-minute walking test is followed by urine and saliva gathering. The qualitative and comparative analysis of the N-acetyl-PGP and PGP in the saliva and urine of the participants on the experiment was made by the signals founded on the mass obtained by the MALDI-TOF/LC-MS/MS. Results: The degradation of the peptides of the collagen, N-acetyl-PGP and PGP, were identified on the samples of urine, 81.2% and 68.7% of the patients with COPD, respectively. Therefore, on the control group, the presence of 11.1% of N-acetyl-PGP and the absence of PGP were identified in the urine of the participants. In the samples of the peptide fractions of the saliva of both groups, the N-acetyl-PGP and PGP were not identified. Conclusion: The presence of N-acetyl-PGP and PGP in the urine of patients with COPD support the hypothesis of the existence of other products of degradation of the components of the ECM yet not identified. Probably only founded in patients with COPD that would help to add information on the diagnostic for the COPD. |
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Silva, Carlos AlbertoCPF:00000000000Costa, Marcilia Silvahttp://lattes.cnpq.br/6704543089433679Bussadori, Sandra KalilCPF:00000000000http://lattes.cnpq.br/4110914499694913CPF:70823057372Rodrigues, Sara Fernanda Albuquerque2015-04-22T17:20:29Z2011-08-062011-01-20RODRIGUES, Sara Fernanda Albuquerque. Caracteriza????o dos pept??deos indicadores de degrada????o do col??geno, n-acetyl-pgp e pgp, na saliva e urina de pacientes com doen??a pulmonar obstrutiva cr??nica.. 2011. 71 f. Disserta????o (Mestrado em Sa??de) - Universidade Nove de Julho, S??o Paulo, 2011.http://bibliotecatede.uninove.br/tede/handle/tede/840The Chronic Obstructive Pulmonary Disease (COPD) is characterized by a chronic limitation of the airflow and an abnormal inflammatory lung response to particles or harmful gases. The COPD is associated with an intense degradation of the extracellular matrix components (ECM) such as the Collagen fibers and Elastin, caused by the imbalance of enzymes in the parenchymal pulmonary. Reports show that the Collagen Hydrolysis produce peptides that are biologically actives (N-acetyl-PGP and PGP) that induces the neutrophil chemo-taxes through the interaction among the receptors CXCR1 and CXCR2 in the bronchoalveolar lavage fluid, and in the blood of the patients with COPD. Objective: To analyze the presence of the N-acetyl-PGP and PGP peptides as specifics indicators of the collagen degradation on the patients with COPD versus the healthy individuals. Methods: The Experiment was done with 25 individuals, grouped under patients with COPD (n=16) and healthy individuals (n=9). After the initial procedures for this experiment, all participants were submitted to the Spectrometric Test. The six-minute walking test is followed by urine and saliva gathering. The qualitative and comparative analysis of the N-acetyl-PGP and PGP in the saliva and urine of the participants on the experiment was made by the signals founded on the mass obtained by the MALDI-TOF/LC-MS/MS. Results: The degradation of the peptides of the collagen, N-acetyl-PGP and PGP, were identified on the samples of urine, 81.2% and 68.7% of the patients with COPD, respectively. Therefore, on the control group, the presence of 11.1% of N-acetyl-PGP and the absence of PGP were identified in the urine of the participants. In the samples of the peptide fractions of the saliva of both groups, the N-acetyl-PGP and PGP were not identified. Conclusion: The presence of N-acetyl-PGP and PGP in the urine of patients with COPD support the hypothesis of the existence of other products of degradation of the components of the ECM yet not identified. Probably only founded in patients with COPD that would help to add information on the diagnostic for the COPD.A doen??a pulmonar obstrutiva cr??nica (DPOC) ?? caracterizada pela limita????o cr??nica do fluxo a??reo associado a uma resposta inflamat??ria anormal dos pulm??es a part??culas ou gases nocivos. A DPOC est?? associada ?? intensa degrada????o dos componentes da matriz extracelular (MEC), como as fibras col??genas e el??sticas, pelo desequil??brio enzim??tico no par??nquima pulmonar. H?? relatos de que a hidr??lise de col??geno produz pept??deos biologicamente ativos (N-acetyl-PGP e PGP) que induzem quimiotaxia de neutr??filos pela via de intera????o com os receptores CXCR1 e CXCR2 no lavado broncoalveolar e sangue de pacientes com DPOC. Objetivos: Analisar a presen??a dos pept??deos N-acetyl-PGP e PGP, como indicadores espec??ficos da degrada????o do col??geno, na urina e saliva de pacientes com DPOC em compara????o ao grupo de indiv??duos saud??veis. M??todos: A amostra foi composta por 25 volunt??rios, agrupados em pacientes com DPOC (n=16) e indiv??duos saud??veis (n=9). Ap??s a triagem inicial, todos realizaram o teste espirom??trico, TC6 e posterior a coleta de urina e saliva. A an??lise qualitativa e comparativa do N-acetyl-PGP e PGP na urina e saliva de cada indiv??duo foram realizadas por sinais de massa obtidas por espectrometria de massas (MALDI-TOF/LC-MS/MS). Resultados: Os pept??deos de degrada????o do col??geno, N-acetyl-PGP e PGP, foram identificados nas amostras de urina de 81,2% e 68,7% dos pacientes com DPOC, respectivamente. Entretanto, verificou-se a presen??a de N-acetyl-PGP em 11,1% nas amostras de urina do grupo controle, mas aus??ncia de PGP. Nas amostras da fra????o pept??dica da saliva de ambos os grupos n??o foram identificados o N-acetyl-PGP e o PGP. Conclus??o: As presen??as de N-acetyl-PGP e PGP na urina de pacientes com DPOC contribuem com a hip??tese de que existem outros produtos de degrada????o dos componentes da MEC, ainda n??o caracterizados, provavelmente, espec??ficos da DPOC e que poderiam auxiliar ou complementar o diagn??stico da doen??a.Made available in DSpace on 2015-04-22T17:20:29Z (GMT). No. of bitstreams: 1 Sara Fernanda Albuquerque Rodrigues.pdf: 3535627 bytes, checksum: be1cfd1cecb88d0f6343449c323f15d6 (MD5) Previous issue date: 2011-01-20application/pdfporUniversidade Nove de JulhoPrograma de P??s-Gradua????o em Ci??ncias da Reabilita????oUNINOVEBRSa??deDPOCPept??deoSalivaUrina.COPDPept??deSalivaUrine.CIENCIAS DA SAUDECaracteriza????o dos pept??deos indicadores de degrada????o do col??geno, N-acetyl-PGP e PGP, na saliva e urina de pacientes com Doen??a Pulmonar Obstrutiva Cr??nicainfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesis8765449414823306929600info:eu-repo/semantics/openAccessreponame:Biblioteca Digital de Teses e Dissertações da Uninoveinstname:Universidade Nove de Julho (UNINOVE)instacron:UNINOVEORIGINALSara Fernanda Albuquerque Rodrigues.pdfapplication/pdf3535627http://localhost:8080/tede/bitstream/tede/840/1/Sara+Fernanda+Albuquerque+Rodrigues.pdfbe1cfd1cecb88d0f6343449c323f15d6MD51tede/8402018-06-15 12:41:31.625oai:localhost:tede/840Biblioteca Digital de Teses e Dissertaçõeshttp://bibliotecatede.uninove.br/PRIhttp://bibliotecatede.uninove.br/oai/requestbibliotecatede@uninove.br||bibliotecatede@uninove.bropendoar:2018-06-15T15:41:31Biblioteca Digital de Teses e Dissertações da Uninove - Universidade Nove de Julho (UNINOVE)false |
dc.title.por.fl_str_mv |
Caracteriza????o dos pept??deos indicadores de degrada????o do col??geno, N-acetyl-PGP e PGP, na saliva e urina de pacientes com Doen??a Pulmonar Obstrutiva Cr??nica |
title |
Caracteriza????o dos pept??deos indicadores de degrada????o do col??geno, N-acetyl-PGP e PGP, na saliva e urina de pacientes com Doen??a Pulmonar Obstrutiva Cr??nica |
spellingShingle |
Caracteriza????o dos pept??deos indicadores de degrada????o do col??geno, N-acetyl-PGP e PGP, na saliva e urina de pacientes com Doen??a Pulmonar Obstrutiva Cr??nica Rodrigues, Sara Fernanda Albuquerque DPOC Pept??deo Saliva Urina. COPD Pept??de Saliva Urine. CIENCIAS DA SAUDE |
title_short |
Caracteriza????o dos pept??deos indicadores de degrada????o do col??geno, N-acetyl-PGP e PGP, na saliva e urina de pacientes com Doen??a Pulmonar Obstrutiva Cr??nica |
title_full |
Caracteriza????o dos pept??deos indicadores de degrada????o do col??geno, N-acetyl-PGP e PGP, na saliva e urina de pacientes com Doen??a Pulmonar Obstrutiva Cr??nica |
title_fullStr |
Caracteriza????o dos pept??deos indicadores de degrada????o do col??geno, N-acetyl-PGP e PGP, na saliva e urina de pacientes com Doen??a Pulmonar Obstrutiva Cr??nica |
title_full_unstemmed |
Caracteriza????o dos pept??deos indicadores de degrada????o do col??geno, N-acetyl-PGP e PGP, na saliva e urina de pacientes com Doen??a Pulmonar Obstrutiva Cr??nica |
title_sort |
Caracteriza????o dos pept??deos indicadores de degrada????o do col??geno, N-acetyl-PGP e PGP, na saliva e urina de pacientes com Doen??a Pulmonar Obstrutiva Cr??nica |
author |
Rodrigues, Sara Fernanda Albuquerque |
author_facet |
Rodrigues, Sara Fernanda Albuquerque |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Silva, Carlos Alberto |
dc.contributor.advisor1ID.fl_str_mv |
CPF:00000000000 |
dc.contributor.referee1.fl_str_mv |
Costa, Marcilia Silva |
dc.contributor.referee1Lattes.fl_str_mv |
http://lattes.cnpq.br/6704543089433679 |
dc.contributor.referee2.fl_str_mv |
Bussadori, Sandra Kalil |
dc.contributor.referee2ID.fl_str_mv |
CPF:00000000000 |
dc.contributor.referee2Lattes.fl_str_mv |
http://lattes.cnpq.br/4110914499694913 |
dc.contributor.authorID.fl_str_mv |
CPF:70823057372 |
dc.contributor.author.fl_str_mv |
Rodrigues, Sara Fernanda Albuquerque |
contributor_str_mv |
Silva, Carlos Alberto Costa, Marcilia Silva Bussadori, Sandra Kalil |
dc.subject.por.fl_str_mv |
DPOC Pept??deo Saliva Urina. |
topic |
DPOC Pept??deo Saliva Urina. COPD Pept??de Saliva Urine. CIENCIAS DA SAUDE |
dc.subject.eng.fl_str_mv |
COPD Pept??de Saliva Urine. |
dc.subject.cnpq.fl_str_mv |
CIENCIAS DA SAUDE |
description |
The Chronic Obstructive Pulmonary Disease (COPD) is characterized by a chronic limitation of the airflow and an abnormal inflammatory lung response to particles or harmful gases. The COPD is associated with an intense degradation of the extracellular matrix components (ECM) such as the Collagen fibers and Elastin, caused by the imbalance of enzymes in the parenchymal pulmonary. Reports show that the Collagen Hydrolysis produce peptides that are biologically actives (N-acetyl-PGP and PGP) that induces the neutrophil chemo-taxes through the interaction among the receptors CXCR1 and CXCR2 in the bronchoalveolar lavage fluid, and in the blood of the patients with COPD. Objective: To analyze the presence of the N-acetyl-PGP and PGP peptides as specifics indicators of the collagen degradation on the patients with COPD versus the healthy individuals. Methods: The Experiment was done with 25 individuals, grouped under patients with COPD (n=16) and healthy individuals (n=9). After the initial procedures for this experiment, all participants were submitted to the Spectrometric Test. The six-minute walking test is followed by urine and saliva gathering. The qualitative and comparative analysis of the N-acetyl-PGP and PGP in the saliva and urine of the participants on the experiment was made by the signals founded on the mass obtained by the MALDI-TOF/LC-MS/MS. Results: The degradation of the peptides of the collagen, N-acetyl-PGP and PGP, were identified on the samples of urine, 81.2% and 68.7% of the patients with COPD, respectively. Therefore, on the control group, the presence of 11.1% of N-acetyl-PGP and the absence of PGP were identified in the urine of the participants. In the samples of the peptide fractions of the saliva of both groups, the N-acetyl-PGP and PGP were not identified. Conclusion: The presence of N-acetyl-PGP and PGP in the urine of patients with COPD support the hypothesis of the existence of other products of degradation of the components of the ECM yet not identified. Probably only founded in patients with COPD that would help to add information on the diagnostic for the COPD. |
publishDate |
2011 |
dc.date.available.fl_str_mv |
2011-08-06 |
dc.date.issued.fl_str_mv |
2011-01-20 |
dc.date.accessioned.fl_str_mv |
2015-04-22T17:20:29Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/masterThesis |
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masterThesis |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
RODRIGUES, Sara Fernanda Albuquerque. Caracteriza????o dos pept??deos indicadores de degrada????o do col??geno, n-acetyl-pgp e pgp, na saliva e urina de pacientes com doen??a pulmonar obstrutiva cr??nica.. 2011. 71 f. Disserta????o (Mestrado em Sa??de) - Universidade Nove de Julho, S??o Paulo, 2011. |
dc.identifier.uri.fl_str_mv |
http://bibliotecatede.uninove.br/tede/handle/tede/840 |
identifier_str_mv |
RODRIGUES, Sara Fernanda Albuquerque. Caracteriza????o dos pept??deos indicadores de degrada????o do col??geno, n-acetyl-pgp e pgp, na saliva e urina de pacientes com doen??a pulmonar obstrutiva cr??nica.. 2011. 71 f. Disserta????o (Mestrado em Sa??de) - Universidade Nove de Julho, S??o Paulo, 2011. |
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http://bibliotecatede.uninove.br/tede/handle/tede/840 |
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