Express?o imunoistoqu?mica dos receptores Toll-like 5 e 9 e do fator de transcri??o Foxp3 em les?es orais de l?quen plano e l?pus eritematoso
Ano de defesa: | 2013 |
---|---|
Autor(a) principal: | |
Orientador(a): | |
Banca de defesa: | |
Tipo de documento: | Dissertação |
Tipo de acesso: | Acesso aberto |
Idioma: | por |
Instituição de defesa: |
Pontif?cia Universidade Cat?lica do Rio Grande do Sul
|
Programa de Pós-Graduação: |
Programa de P?s-Gradua??o em Odontologia
|
Departamento: |
Faculdade de Odontologia
|
País: |
BR
|
Palavras-chave em Português: | |
Área do conhecimento CNPq: | |
Link de acesso: | http://tede2.pucrs.br/tede2/handle/tede/1216 |
Resumo: | The present study aimed at analyzing the expression of toll-like receptors (TLR) 5 and 9 and forkhead box p3 regulatory T cells transcription factor (Foxp3) in oral lesions of lichen planus and lupus erythematosus. Twenty-one biopsy specimens of lichen planus, 21 of lupus erythematosus and 21 of inflammatory fibrous hyperplasia (control group) were subjected to immunohistochemical staining with anti-TLR5, anti-TLR-9 and anti-Foxp3, whose expressions were quantified using Image Pro Plus 4.5.1 software (Media Cybernetics). Immunostaining for Foxp3 differed significantly between the three groups, where the highest levels were shown by lichen planus and the lowest ones by controls. TLR5 expression did not differ significantly between lupus erythematosus and control group, but it was significantly greater in these two groups than in lichen planus. When we considered the two types of lupus separately, there was no significant difference of TLR5 between lichen planus and systemic lupus erythematosus, but discoid lupus erythematosus as well as the control group showed significantly greater values than lichen planus. TLR9 immunostaining did not differ significantly between the groups analyzed. A weak negative correlation was observed between Foxp3 and TLR5 (r= -0.279), while there was no other correlation. When this analysis was performed within each group, no correlation was observed between the variables. In conclusion, Foxp3 expression is increased in oral lesions of lichen planus and lupus erythematosus, whereas TLR5 and TLR9 do not show increased expression in these lesions. Foxp3 and TLR5 are inversely correlated. Further investigations analyzing Treg cells and proinflammatory cytokines in these diseases are warranted. |
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Cherubini, KarenCPF:55371400044http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4795257U2CPF:01836785089http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4471634A9Trucci, Victoria Martina2015-04-14T13:30:25Z2013-05-282013-03-22TRUCCI, Victoria Martina. Express?o imunoistoqu?mica dos receptores Toll-like 5 e 9 e do fator de transcri??o Foxp3 em les?es orais de l?quen plano e l?pus eritematoso. 2013. 97 f. Disserta??o (Mestrado em Odontologia) - Pontif?cia Universidade Cat?lica do Rio Grande do Sul, Porto Alegre, 2013.http://tede2.pucrs.br/tede2/handle/tede/1216The present study aimed at analyzing the expression of toll-like receptors (TLR) 5 and 9 and forkhead box p3 regulatory T cells transcription factor (Foxp3) in oral lesions of lichen planus and lupus erythematosus. Twenty-one biopsy specimens of lichen planus, 21 of lupus erythematosus and 21 of inflammatory fibrous hyperplasia (control group) were subjected to immunohistochemical staining with anti-TLR5, anti-TLR-9 and anti-Foxp3, whose expressions were quantified using Image Pro Plus 4.5.1 software (Media Cybernetics). Immunostaining for Foxp3 differed significantly between the three groups, where the highest levels were shown by lichen planus and the lowest ones by controls. TLR5 expression did not differ significantly between lupus erythematosus and control group, but it was significantly greater in these two groups than in lichen planus. When we considered the two types of lupus separately, there was no significant difference of TLR5 between lichen planus and systemic lupus erythematosus, but discoid lupus erythematosus as well as the control group showed significantly greater values than lichen planus. TLR9 immunostaining did not differ significantly between the groups analyzed. A weak negative correlation was observed between Foxp3 and TLR5 (r= -0.279), while there was no other correlation. When this analysis was performed within each group, no correlation was observed between the variables. In conclusion, Foxp3 expression is increased in oral lesions of lichen planus and lupus erythematosus, whereas TLR5 and TLR9 do not show increased expression in these lesions. Foxp3 and TLR5 are inversely correlated. Further investigations analyzing Treg cells and proinflammatory cytokines in these diseases are warranted.O presente estudo teve por objetivo avaliar o padr?o de express?o dos receptores toll-like (TLR) 5 e 9 e do fator de transcri??o forkhead box p3 (Foxp3) de c?lulas T regulat?rias em les?es orais de l?quen plano e l?pus eritematoso. Vinte e um esp?cimes de bi?psias de l?quen plano, 21 de l?pus eritematoso e 21 de hiperplasia fibrosa inflamat?ria (grupo-controle) foram submetidos a processamento imunoistoqu?mico com os marcadores anti-TLR5, anti-TLR9 e anti-Foxp3, e a quantifica??o da express?o desses marcadores foi realizada por meio do software Image Pro Plus 4.5.1 (Media Cybernetics). O padr?o de express?o do Foxp3 diferiu significativamente entre os tr?s grupos, sendo os maiores n?veis observados no grupo l?quen plano, e os menores, no grupo-controle. A express?o do TLR5 n?o diferiu significativamente entre os grupos l?pus eritematoso e controle, mas foi maior nesses grupos quando comparados ao grupo l?quen plano. Ao discriminarem-se os subtipos de l?pus eritematoso sist?mico e discoide, n?o foi verificada diferen?a significativa na express?o do TLR5 entre l?quen plano e l?pus sist?mico, mas foram verificados maiores n?veis desse marcador no l?pus discoide e no grupo-controle quando comparados ao grupo l?quen plano. O padr?o de express?o do TLR9 n?o diferiu significativamente entre os grupos. Foxp3 e TLR5 apresentaram correla??o negativa fraca (r= -0.279). As demais vari?veis n?o apresentaram correla??o, mesmo quando analisadas intragrupo. Os resultados do presente estudo permitem concluir que a express?o de Foxp3 est? aumentada em les?es orais de l?quen plano e l?pus eritematoso, enquanto TLR5 e TLR9 n?o exibem maior express?o nessas les?es; Foxp3 e TLR5 est?o inversamente correlacionados. Novas investiga??es sobre c?lulas T regulat?rias, receptores toll-like e citocinas pr?-inflamat?rias fazem-se necess?rias para o melhor entendimento de doen?as como l?quen plano e l?pus eritematoso.Made available in DSpace on 2015-04-14T13:30:25Z (GMT). No. of bitstreams: 1 448387.pdf: 2406290 bytes, checksum: b9c686175b2c7a33644bea96bc032d9a (MD5) Previous issue date: 2013-03-22application/pdfhttp://tede2.pucrs.br:80/tede2/retrieve/8287/448387.pdf.jpgporPontif?cia Universidade Cat?lica do Rio Grande do SulPrograma de P?s-Gradua??o em OdontologiaPUCRSBRFaculdade de OdontologiaODONTOLOGIAESTOMATOLOGIA CL?NICASISTEMA IMUNOL?GICOIMUNOISTOQU?MICAL?QUEN PLANO BUCALLUPUS ERITEMATOSO SIST?MICOCNPQ::CIENCIAS DA SAUDE::ODONTOLOGIAExpress?o imunoistoqu?mica dos receptores Toll-like 5 e 9 e do fator de transcri??o Foxp3 em les?es orais de l?quen plano e l?pus eritematosoinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesis-80965548187336651645006004673435736271820140info:eu-repo/semantics/openAccessreponame:Biblioteca Digital de Teses e Dissertações da PUC_RSinstname:Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS)instacron:PUC_RSTHUMBNAIL448387.pdf.jpg448387.pdf.jpgimage/jpeg2824http://tede2.pucrs.br/tede2/bitstream/tede/1216/3/448387.pdf.jpg17dad663072e7d1601c1c87fb4e6ea1fMD53TEXT448387.pdf.txt448387.pdf.txttext/plain138300http://tede2.pucrs.br/tede2/bitstream/tede/1216/2/448387.pdf.txtb6bf57098003a9dedac1fa68dc49ddd8MD52ORIGINAL448387.pdfapplication/pdf2406290http://tede2.pucrs.br/tede2/bitstream/tede/1216/1/448387.pdfb9c686175b2c7a33644bea96bc032d9aMD51tede/12162015-04-17 17:16:25.377oai:tede2.pucrs.br:tede/1216Biblioteca Digital de Teses e Dissertaçõeshttp://tede2.pucrs.br/tede2/PRIhttps://tede2.pucrs.br/oai/requestbiblioteca.central@pucrs.br||opendoar:2015-04-17T20:16:25Biblioteca Digital de Teses e Dissertações da PUC_RS - Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS)false |
dc.title.por.fl_str_mv |
Express?o imunoistoqu?mica dos receptores Toll-like 5 e 9 e do fator de transcri??o Foxp3 em les?es orais de l?quen plano e l?pus eritematoso |
title |
Express?o imunoistoqu?mica dos receptores Toll-like 5 e 9 e do fator de transcri??o Foxp3 em les?es orais de l?quen plano e l?pus eritematoso |
spellingShingle |
Express?o imunoistoqu?mica dos receptores Toll-like 5 e 9 e do fator de transcri??o Foxp3 em les?es orais de l?quen plano e l?pus eritematoso Trucci, Victoria Martina ODONTOLOGIA ESTOMATOLOGIA CL?NICA SISTEMA IMUNOL?GICO IMUNOISTOQU?MICA L?QUEN PLANO BUCAL LUPUS ERITEMATOSO SIST?MICO CNPQ::CIENCIAS DA SAUDE::ODONTOLOGIA |
title_short |
Express?o imunoistoqu?mica dos receptores Toll-like 5 e 9 e do fator de transcri??o Foxp3 em les?es orais de l?quen plano e l?pus eritematoso |
title_full |
Express?o imunoistoqu?mica dos receptores Toll-like 5 e 9 e do fator de transcri??o Foxp3 em les?es orais de l?quen plano e l?pus eritematoso |
title_fullStr |
Express?o imunoistoqu?mica dos receptores Toll-like 5 e 9 e do fator de transcri??o Foxp3 em les?es orais de l?quen plano e l?pus eritematoso |
title_full_unstemmed |
Express?o imunoistoqu?mica dos receptores Toll-like 5 e 9 e do fator de transcri??o Foxp3 em les?es orais de l?quen plano e l?pus eritematoso |
title_sort |
Express?o imunoistoqu?mica dos receptores Toll-like 5 e 9 e do fator de transcri??o Foxp3 em les?es orais de l?quen plano e l?pus eritematoso |
author |
Trucci, Victoria Martina |
author_facet |
Trucci, Victoria Martina |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Cherubini, Karen |
dc.contributor.advisor1ID.fl_str_mv |
CPF:55371400044 |
dc.contributor.advisor1Lattes.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4795257U2 |
dc.contributor.authorID.fl_str_mv |
CPF:01836785089 |
dc.contributor.authorLattes.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4471634A9 |
dc.contributor.author.fl_str_mv |
Trucci, Victoria Martina |
contributor_str_mv |
Cherubini, Karen |
dc.subject.por.fl_str_mv |
ODONTOLOGIA ESTOMATOLOGIA CL?NICA SISTEMA IMUNOL?GICO IMUNOISTOQU?MICA L?QUEN PLANO BUCAL LUPUS ERITEMATOSO SIST?MICO |
topic |
ODONTOLOGIA ESTOMATOLOGIA CL?NICA SISTEMA IMUNOL?GICO IMUNOISTOQU?MICA L?QUEN PLANO BUCAL LUPUS ERITEMATOSO SIST?MICO CNPQ::CIENCIAS DA SAUDE::ODONTOLOGIA |
dc.subject.cnpq.fl_str_mv |
CNPQ::CIENCIAS DA SAUDE::ODONTOLOGIA |
description |
The present study aimed at analyzing the expression of toll-like receptors (TLR) 5 and 9 and forkhead box p3 regulatory T cells transcription factor (Foxp3) in oral lesions of lichen planus and lupus erythematosus. Twenty-one biopsy specimens of lichen planus, 21 of lupus erythematosus and 21 of inflammatory fibrous hyperplasia (control group) were subjected to immunohistochemical staining with anti-TLR5, anti-TLR-9 and anti-Foxp3, whose expressions were quantified using Image Pro Plus 4.5.1 software (Media Cybernetics). Immunostaining for Foxp3 differed significantly between the three groups, where the highest levels were shown by lichen planus and the lowest ones by controls. TLR5 expression did not differ significantly between lupus erythematosus and control group, but it was significantly greater in these two groups than in lichen planus. When we considered the two types of lupus separately, there was no significant difference of TLR5 between lichen planus and systemic lupus erythematosus, but discoid lupus erythematosus as well as the control group showed significantly greater values than lichen planus. TLR9 immunostaining did not differ significantly between the groups analyzed. A weak negative correlation was observed between Foxp3 and TLR5 (r= -0.279), while there was no other correlation. When this analysis was performed within each group, no correlation was observed between the variables. In conclusion, Foxp3 expression is increased in oral lesions of lichen planus and lupus erythematosus, whereas TLR5 and TLR9 do not show increased expression in these lesions. Foxp3 and TLR5 are inversely correlated. Further investigations analyzing Treg cells and proinflammatory cytokines in these diseases are warranted. |
publishDate |
2013 |
dc.date.available.fl_str_mv |
2013-05-28 |
dc.date.issued.fl_str_mv |
2013-03-22 |
dc.date.accessioned.fl_str_mv |
2015-04-14T13:30:25Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
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masterThesis |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
TRUCCI, Victoria Martina. Express?o imunoistoqu?mica dos receptores Toll-like 5 e 9 e do fator de transcri??o Foxp3 em les?es orais de l?quen plano e l?pus eritematoso. 2013. 97 f. Disserta??o (Mestrado em Odontologia) - Pontif?cia Universidade Cat?lica do Rio Grande do Sul, Porto Alegre, 2013. |
dc.identifier.uri.fl_str_mv |
http://tede2.pucrs.br/tede2/handle/tede/1216 |
identifier_str_mv |
TRUCCI, Victoria Martina. Express?o imunoistoqu?mica dos receptores Toll-like 5 e 9 e do fator de transcri??o Foxp3 em les?es orais de l?quen plano e l?pus eritematoso. 2013. 97 f. Disserta??o (Mestrado em Odontologia) - Pontif?cia Universidade Cat?lica do Rio Grande do Sul, Porto Alegre, 2013. |
url |
http://tede2.pucrs.br/tede2/handle/tede/1216 |
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