Compara??o do efeito do plasma rico em plaquetas e fibrina rica em plaquetas no reparo do tend?o de Aquiles em ratos
Ano de defesa: | 2012 |
---|---|
Autor(a) principal: | |
Orientador(a): | |
Banca de defesa: | |
Tipo de documento: | Dissertação |
Tipo de acesso: | Acesso aberto |
Idioma: | por |
Instituição de defesa: |
Pontif?cia Universidade Cat?lica do Rio Grande do Sul
|
Programa de Pós-Graduação: |
Programa de P?s-Gradua??o em Medicina e Ci?ncias da Sa?de
|
Departamento: |
Faculdade de Medicina
|
País: |
BR
|
Palavras-chave em Português: | |
Área do conhecimento CNPq: | |
Link de acesso: | http://tede2.pucrs.br/tede2/handle/tede/1683 |
Resumo: | OBJECTIVE : To evaluate and compare the effect of using Platelet-Rich Plasma (PRP) and Platelet-Rich Fibrin (PRF) in the repair of the Achilles tendon (TA) of rats. MATERIAL and METHODS : Forty-eight adult male rats randomized into three experimental groups: PRP, PRF and Control (saline 0.9%). A 3 cm longitudinal incision was made in the right leg of each rat in order to expose the TA. Later, it was a break in the same horizontal, and the repair performed with suture Kessler-type, when the sequence was applied 50 μL of the treatment in question. After 14 and 28 days postoperatively, the targeted part of the TA was removed, thus initializing the histological processing (Hematoxylin/Eosin; Sirius Red) to obtain results. The data obtained were expressed at 5% significance level (p <0.05) with mean ? SE. RESULTS : When comparing the three groups together, as to time and area of collagen type I and III, only statistical difference between the control group (16.22%) and the PRP group (37.16%) at 14 days after the treatment, no statistically significant difference was observed in the other groups. In the same way, when compared within groups of 14 and 28 days, the PRF group showed a significant difference for collagen I (27.76% and 47.74%) and III (66.94% and 46%) respectively. Control group showed significant difference in collagen type I (14.20% and 40.90%), not observed any significant difference in the PRP group. When evaluated on Hematoxylin/Eosin staining, PRP and control were similar in the times studied, differing only in the presence of hemorrhage, that was more expressed in the PRP group. Already the group PRF has shown to be more organized, especially in time of 28 days compared to the other two treatments. CONCLUSION : The data from this study suggest that the Platelet-Rich Fibrin tends to promote the accelerated regeneration of the Achilles tendon of rats, bringing promising prospects for future use in the clinic. |
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Silva, Jefferson Luis Braga daCPF:32077491000http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4707540D6CPF:01084144000http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4463309Y7Dietrich, Franciele2015-04-14T13:35:30Z2012-05-042012-03-28DIETRICH, Franciele. Compara??o do efeito do plasma rico em plaquetas e fibrina rica em plaquetas no reparo do tend?o de Aquiles em ratos. 2012. 104 f. Disserta??o (Mestrado em Medicina e Ci?ncias da Sa?de) - Pontif?cia Universidade Cat?lica do Rio Grande do Sul, Porto Alegre, 2012.http://tede2.pucrs.br/tede2/handle/tede/1683OBJECTIVE : To evaluate and compare the effect of using Platelet-Rich Plasma (PRP) and Platelet-Rich Fibrin (PRF) in the repair of the Achilles tendon (TA) of rats. MATERIAL and METHODS : Forty-eight adult male rats randomized into three experimental groups: PRP, PRF and Control (saline 0.9%). A 3 cm longitudinal incision was made in the right leg of each rat in order to expose the TA. Later, it was a break in the same horizontal, and the repair performed with suture Kessler-type, when the sequence was applied 50 μL of the treatment in question. After 14 and 28 days postoperatively, the targeted part of the TA was removed, thus initializing the histological processing (Hematoxylin/Eosin; Sirius Red) to obtain results. The data obtained were expressed at 5% significance level (p <0.05) with mean ? SE. RESULTS : When comparing the three groups together, as to time and area of collagen type I and III, only statistical difference between the control group (16.22%) and the PRP group (37.16%) at 14 days after the treatment, no statistically significant difference was observed in the other groups. In the same way, when compared within groups of 14 and 28 days, the PRF group showed a significant difference for collagen I (27.76% and 47.74%) and III (66.94% and 46%) respectively. Control group showed significant difference in collagen type I (14.20% and 40.90%), not observed any significant difference in the PRP group. When evaluated on Hematoxylin/Eosin staining, PRP and control were similar in the times studied, differing only in the presence of hemorrhage, that was more expressed in the PRP group. Already the group PRF has shown to be more organized, especially in time of 28 days compared to the other two treatments. CONCLUSION : The data from this study suggest that the Platelet-Rich Fibrin tends to promote the accelerated regeneration of the Achilles tendon of rats, bringing promising prospects for future use in the clinic.OBJETIVO : Avaliar e comparar o efeito do uso de plasma rico em plaquetas (PRP) e fibrina rica em plaquetas (PRF) no reparo do tend?o de Aquiles (TA) de ratos. MATERIAL e M?TODOS : Foram utilizados 48 ratos machos adultos distribu?dos randomicamente em tr?s grupos experimentais: PRP, PRF e Controle (solu??o salina 0,9%). Uma incis?o longitudinal de 3 cm foi realizada na perna direita de cada rato, a fim de expor o TA. Posteriormente, fez-se uma ruptura horizontal no mesmo, sendo a repara??o realizada com sutura do tipo Kessler, onde na seq??ncia foi aplicado 50 μL do tratamento em quest?o. Passados 14 e 28 dias p?s- operat?rio, a parte segmentada do TA foi removida, inicializando assim o processo histol?gico (Hematoxilina/Eosina; Picros?rius Red) para obten??o de resultados. Os dados obtidos foram expressos ao n?vel de 5% de signific?ncia (p < 0.05) com m?dia ? EP. RESULTADOS : Ao comparar os tr?s grupos entre si, quanto ao tempo e ?rea de col?geno tipo I e III, houve diferen?a estat?stica somente entre o grupo Controle (16.22%) e o grupo PRP (37.16%) aos 14 dias ap?s o tratamento, n?o sendo observada diferen?a estatisticamente significativa nos outros grupos. Da mesma forma, quando comparados intra-grupos em 14 e 28 dias, o grupo PRF apresentou diferen?a significativa para col?geno I (27.76% e 47.74%) e III (66.94% e 46%) respectivamente. Grupo Controle demonstrou diferen?a significativa para col?geno tipo I (14.20% e 40.90%), n?o sendo observada qualquer diferen?a significativa no grupo PRP. Quando avaliados na colora??o Hematoxilina e Eosina, PRP e Controle apresentaram-se semelhantes nos tempos avaliados, diferindo apenas quanto a presen?a de hemorragia, que foi mais expressa no grupo PRP. J? o grupo PRF demonstrou-se mais organizado, principalmente no tempo de 28 dias quando comparado aos outros dois tratamentos. CONCLUS?O : Os dados obtidos neste trabalho sugerem que a Fibrina Rica em Plaquetas tem tend?ncia a promover a regenera??o acelerada do tend?o de Aquiles de ratos, trazendo perspectivas promissoras para futuras utiliza??es na cl?nica.Made available in DSpace on 2015-04-14T13:35:30Z (GMT). No. of bitstreams: 1 438171.pdf: 6920524 bytes, checksum: f5e5c6d7e177dfd60f3b3303552cc797 (MD5) Previous issue date: 2012-03-28application/pdfhttp://tede2.pucrs.br:80/tede2/retrieve/9262/438171.pdf.jpgporPontif?cia Universidade Cat?lica do Rio Grande do SulPrograma de P?s-Gradua??o em Medicina e Ci?ncias da Sa?dePUCRSBRFaculdade de MedicinaMEDICINAREGENERA??O TECIDUAL GUIADAPLASMATEND?O DO CALC?NEOCNPQ::CIENCIAS DA SAUDE::MEDICINACompara??o do efeito do plasma rico em plaquetas e fibrina rica em plaquetas no reparo do tend?o de Aquiles em ratosinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesis7620745074616285884500600-8624664729441623247info:eu-repo/semantics/openAccessreponame:Biblioteca Digital de Teses e Dissertações da PUC_RSinstname:Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS)instacron:PUC_RSTHUMBNAIL438171.pdf.jpg438171.pdf.jpgimage/jpeg3870http://tede2.pucrs.br/tede2/bitstream/tede/1683/3/438171.pdf.jpg65dae333453fc5b92dd21fe98f9ebb26MD53TEXT438171.pdf.txt438171.pdf.txttext/plain165917http://tede2.pucrs.br/tede2/bitstream/tede/1683/2/438171.pdf.txtac845c8af4705f7cd65f251038e9b35dMD52ORIGINAL438171.pdfapplication/pdf6920524http://tede2.pucrs.br/tede2/bitstream/tede/1683/1/438171.pdff5e5c6d7e177dfd60f3b3303552cc797MD51tede/16832015-04-17 17:04:42.917oai:tede2.pucrs.br:tede/1683Biblioteca Digital de Teses e Dissertaçõeshttp://tede2.pucrs.br/tede2/PRIhttps://tede2.pucrs.br/oai/requestbiblioteca.central@pucrs.br||opendoar:2015-04-17T20:04:42Biblioteca Digital de Teses e Dissertações da PUC_RS - Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS)false |
dc.title.por.fl_str_mv |
Compara??o do efeito do plasma rico em plaquetas e fibrina rica em plaquetas no reparo do tend?o de Aquiles em ratos |
title |
Compara??o do efeito do plasma rico em plaquetas e fibrina rica em plaquetas no reparo do tend?o de Aquiles em ratos |
spellingShingle |
Compara??o do efeito do plasma rico em plaquetas e fibrina rica em plaquetas no reparo do tend?o de Aquiles em ratos Dietrich, Franciele MEDICINA REGENERA??O TECIDUAL GUIADA PLASMA TEND?O DO CALC?NEO CNPQ::CIENCIAS DA SAUDE::MEDICINA |
title_short |
Compara??o do efeito do plasma rico em plaquetas e fibrina rica em plaquetas no reparo do tend?o de Aquiles em ratos |
title_full |
Compara??o do efeito do plasma rico em plaquetas e fibrina rica em plaquetas no reparo do tend?o de Aquiles em ratos |
title_fullStr |
Compara??o do efeito do plasma rico em plaquetas e fibrina rica em plaquetas no reparo do tend?o de Aquiles em ratos |
title_full_unstemmed |
Compara??o do efeito do plasma rico em plaquetas e fibrina rica em plaquetas no reparo do tend?o de Aquiles em ratos |
title_sort |
Compara??o do efeito do plasma rico em plaquetas e fibrina rica em plaquetas no reparo do tend?o de Aquiles em ratos |
author |
Dietrich, Franciele |
author_facet |
Dietrich, Franciele |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Silva, Jefferson Luis Braga da |
dc.contributor.advisor1ID.fl_str_mv |
CPF:32077491000 |
dc.contributor.advisor1Lattes.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4707540D6 |
dc.contributor.authorID.fl_str_mv |
CPF:01084144000 |
dc.contributor.authorLattes.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4463309Y7 |
dc.contributor.author.fl_str_mv |
Dietrich, Franciele |
contributor_str_mv |
Silva, Jefferson Luis Braga da |
dc.subject.por.fl_str_mv |
MEDICINA REGENERA??O TECIDUAL GUIADA PLASMA TEND?O DO CALC?NEO |
topic |
MEDICINA REGENERA??O TECIDUAL GUIADA PLASMA TEND?O DO CALC?NEO CNPQ::CIENCIAS DA SAUDE::MEDICINA |
dc.subject.cnpq.fl_str_mv |
CNPQ::CIENCIAS DA SAUDE::MEDICINA |
description |
OBJECTIVE : To evaluate and compare the effect of using Platelet-Rich Plasma (PRP) and Platelet-Rich Fibrin (PRF) in the repair of the Achilles tendon (TA) of rats. MATERIAL and METHODS : Forty-eight adult male rats randomized into three experimental groups: PRP, PRF and Control (saline 0.9%). A 3 cm longitudinal incision was made in the right leg of each rat in order to expose the TA. Later, it was a break in the same horizontal, and the repair performed with suture Kessler-type, when the sequence was applied 50 μL of the treatment in question. After 14 and 28 days postoperatively, the targeted part of the TA was removed, thus initializing the histological processing (Hematoxylin/Eosin; Sirius Red) to obtain results. The data obtained were expressed at 5% significance level (p <0.05) with mean ? SE. RESULTS : When comparing the three groups together, as to time and area of collagen type I and III, only statistical difference between the control group (16.22%) and the PRP group (37.16%) at 14 days after the treatment, no statistically significant difference was observed in the other groups. In the same way, when compared within groups of 14 and 28 days, the PRF group showed a significant difference for collagen I (27.76% and 47.74%) and III (66.94% and 46%) respectively. Control group showed significant difference in collagen type I (14.20% and 40.90%), not observed any significant difference in the PRP group. When evaluated on Hematoxylin/Eosin staining, PRP and control were similar in the times studied, differing only in the presence of hemorrhage, that was more expressed in the PRP group. Already the group PRF has shown to be more organized, especially in time of 28 days compared to the other two treatments. CONCLUSION : The data from this study suggest that the Platelet-Rich Fibrin tends to promote the accelerated regeneration of the Achilles tendon of rats, bringing promising prospects for future use in the clinic. |
publishDate |
2012 |
dc.date.available.fl_str_mv |
2012-05-04 |
dc.date.issued.fl_str_mv |
2012-03-28 |
dc.date.accessioned.fl_str_mv |
2015-04-14T13:35:30Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/masterThesis |
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publishedVersion |
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DIETRICH, Franciele. Compara??o do efeito do plasma rico em plaquetas e fibrina rica em plaquetas no reparo do tend?o de Aquiles em ratos. 2012. 104 f. Disserta??o (Mestrado em Medicina e Ci?ncias da Sa?de) - Pontif?cia Universidade Cat?lica do Rio Grande do Sul, Porto Alegre, 2012. |
dc.identifier.uri.fl_str_mv |
http://tede2.pucrs.br/tede2/handle/tede/1683 |
identifier_str_mv |
DIETRICH, Franciele. Compara??o do efeito do plasma rico em plaquetas e fibrina rica em plaquetas no reparo do tend?o de Aquiles em ratos. 2012. 104 f. Disserta??o (Mestrado em Medicina e Ci?ncias da Sa?de) - Pontif?cia Universidade Cat?lica do Rio Grande do Sul, Porto Alegre, 2012. |
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http://tede2.pucrs.br/tede2/handle/tede/1683 |
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PUCRS |
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BR |
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Pontif?cia Universidade Cat?lica do Rio Grande do Sul |
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