Efeito do plasma rico em plaquetas e da fibrina rica em plaquetas na cicatriza??o de feridas cut?neas em ratos
Ano de defesa: | 2013 |
---|---|
Autor(a) principal: | |
Orientador(a): | |
Banca de defesa: | |
Tipo de documento: | Dissertação |
Tipo de acesso: | Acesso aberto |
Idioma: | por |
Instituição de defesa: |
Pontif?cia Universidade Cat?lica do Rio Grande do Sul
|
Programa de Pós-Graduação: |
Programa de P?s-Gradua??o em Medicina e Ci?ncias da Sa?de
|
Departamento: |
Faculdade de Medicina
|
País: |
BR
|
Palavras-chave em Português: | |
Área do conhecimento CNPq: | |
Link de acesso: | http://tede2.pucrs.br/tede2/handle/tede/1750 |
Resumo: | Objective: To evaluate the effect of platelet-rich plasma (PRP) and platelet-rich fibrin (PRF) on cutaneous wound healing in rats by comparing treated and control wounds. Material and Methods: Forty-eight Wistar rats were used in the study. Two wounds with 1-cm diameter, of total skin thickness, were excised from the back of the animals and treated with either PRP or PRF and saline (paired model). The wounds were evaluated immediately after the procedure and after 7 and 14 days using macroscopic (photographs) and microscopic (histology) methods. The photographs were used to measure the area of wounds in order to analyse wound contraction (morphometric analysis). The histological images permitted a count-analysis of collagen concentration (histomorphometric analysis) while the histological slides providet the description of the wounds characteristics. Results: Morphometric analysis revealed statistically significant time effect (P<0.001) and treatment group effect (P=0.022) over the observation period. The rate of wound contraction was higher in PRF-treated wounds than in PRP-treated wounds (P=0.030). In the histomorphometric analysis, mean collagen concentration, on the images of the experimental groups, increased significantly (P<0.001) from day 7 to day 14. There was wide variation in the percentage of collagen concentration: 129% in the PRF group vs. 22% in the PRP group; however, this difference did not achieve statistical significance (P=0.380). Histology of the wounds suggested a more accelerated healing process in the PRF group vs. the PRP group, despite the lack of statistical significance in the characteristics analyzed. PRP-treated and PRF-treated groups were not statistically superior to their controls in any of the studied variables. Conclusion: The present results suggest that treatment with PRP and PRF were not more effective than saline for cutaneous wound healing in rats. However, PRF proved to be superior to PRP. |
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Silva, Jefferson Luis Braga daCPF:32077491000http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4707540D6CPF:50978802004http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4250720U8Camargo, Fernanda Ferreira de2015-04-14T13:35:49Z2013-11-112013-08-08CAMARGO, Fernanda Ferreira de. Efeito do plasma rico em plaquetas e da fibrina rica em plaquetas na cicatriza??o de feridas cut?neas em ratos. 2013. 184 f. Disserta??o (Mestrado em Medicina e Ci?ncias da Sa?de) - Pontif?cia Universidade Cat?lica do Rio Grande do Sul, Porto Alegre, 2013.http://tede2.pucrs.br/tede2/handle/tede/1750Objective: To evaluate the effect of platelet-rich plasma (PRP) and platelet-rich fibrin (PRF) on cutaneous wound healing in rats by comparing treated and control wounds. Material and Methods: Forty-eight Wistar rats were used in the study. Two wounds with 1-cm diameter, of total skin thickness, were excised from the back of the animals and treated with either PRP or PRF and saline (paired model). The wounds were evaluated immediately after the procedure and after 7 and 14 days using macroscopic (photographs) and microscopic (histology) methods. The photographs were used to measure the area of wounds in order to analyse wound contraction (morphometric analysis). The histological images permitted a count-analysis of collagen concentration (histomorphometric analysis) while the histological slides providet the description of the wounds characteristics. Results: Morphometric analysis revealed statistically significant time effect (P<0.001) and treatment group effect (P=0.022) over the observation period. The rate of wound contraction was higher in PRF-treated wounds than in PRP-treated wounds (P=0.030). In the histomorphometric analysis, mean collagen concentration, on the images of the experimental groups, increased significantly (P<0.001) from day 7 to day 14. There was wide variation in the percentage of collagen concentration: 129% in the PRF group vs. 22% in the PRP group; however, this difference did not achieve statistical significance (P=0.380). Histology of the wounds suggested a more accelerated healing process in the PRF group vs. the PRP group, despite the lack of statistical significance in the characteristics analyzed. PRP-treated and PRF-treated groups were not statistically superior to their controls in any of the studied variables. Conclusion: The present results suggest that treatment with PRP and PRF were not more effective than saline for cutaneous wound healing in rats. However, PRF proved to be superior to PRP.Objetivo: Avaliar o efeito do Plasma Rico em Plaquetas (PRP) e da Fibrina Rica em Plaquetas (PRF) na cicatriza??o de feridas cut?neas em ratos, comparando os tratamentos entre si e com seus respectivos grupos controle. Material e M?todos: Foram utilizados 48 ratos Wistar. Duas feridas de 1,0 cm de di?metro, de espessura total da pele, foram excisadas do dorso dos animais e tratadas com PRP ou PRF e soro fisiol?gico (modelo emparelhado). As feridas foram avaliadas no p?s-operat?rio imediato e depois de 7 e 14 dias, de forma macrosc?pica (fotografias) e microsc?pica (histologia). As fotografias forneceram medidas da ?rea das feridas, possibilitando analisar sua contra??o (an?lise morfom?trica). As imagens histol?gicas permitiram calcular a concentra??o de col?geno nas feridas (an?lise histomorfom?trica) e as l?minas histol?gicas a descrever suas caracter?sticas. Resultados: Quanto ? an?lise morfom?trica, o efeito do tempo (P<0,001) e dos grupos (P=0,022), ao longo do per?odo de observa??o, foi estatisticamente significativo. A taxa de contra??o das feridas tratadas com PRF foi maior do que as tratadas com PRP (P=0,030). Na an?lise histomorfom?trica, a m?dia da concentra??o de col?geno, nas imagens dos grupos experimentais, apresentou um aumento do 7? para o 14? dia, considerado significativo (P<0,001). Houve grande varia??o percentual na concentra??o de col?geno: 129% para a PRF contra 22% para o PRP, por?m, sem signific?ncia estat?stica (P=0,380). A descri??o histol?gica das feridas sugeriu que o grupo PRF apresentou maior acelera??o do reparo quando comparado ao grupo PRP, apesar da aus?ncia de signific?ncia estat?stica nas caracter?sticas avaliadas. Os grupos de tratamento PRP e PRF n?o foram estatisticamente superiores aos seus grupos controle nas vari?veis estudadas. Conclus?o: Os dados do presente estudo sugerem que o PRP e a PRF n?o apresentaram resultados mais eficazes do que o soro fisiol?gico na cicatriza??o de feridas cut?neas em ratos. No entanto, a PRF mostrou-se superior ao PRP.Made available in DSpace on 2015-04-14T13:35:49Z (GMT). No. of bitstreams: 1 452038.pdf: 5926182 bytes, checksum: 5375eb92e47f0a2a30ef9fa65dc549c5 (MD5) Previous issue date: 2013-08-08application/pdfhttp://tede2.pucrs.br:80/tede2/retrieve/9274/452038.pdf.jpgporPontif?cia Universidade Cat?lica do Rio Grande do SulPrograma de P?s-Gradua??o em Medicina e Ci?ncias da Sa?dePUCRSBRFaculdade de MedicinaMEDICINACICATRIZA??O DE FERIDASPLASMAPLAQUETASREGENERA??O TECIDUAL GUIADACNPQ::CIENCIAS DA SAUDE::MEDICINAEfeito do plasma rico em plaquetas e da fibrina rica em plaquetas na cicatriza??o de feridas cut?neas em ratosinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesis7620745074616285884500600-8624664729441623247info:eu-repo/semantics/openAccessreponame:Biblioteca Digital de Teses e Dissertações da PUC_RSinstname:Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS)instacron:PUC_RSTHUMBNAIL452038.pdf.jpg452038.pdf.jpgimage/jpeg3238http://tede2.pucrs.br/tede2/bitstream/tede/1750/3/452038.pdf.jpg16338fe63e0aa017743021bd6f0c8683MD53TEXT452038.pdf.txt452038.pdf.txttext/plain293564http://tede2.pucrs.br/tede2/bitstream/tede/1750/2/452038.pdf.txtcfd89b4d1c0be4ddc6c4d164fd44b109MD52ORIGINAL452038.pdfapplication/pdf5926182http://tede2.pucrs.br/tede2/bitstream/tede/1750/1/452038.pdf5375eb92e47f0a2a30ef9fa65dc549c5MD51tede/17502015-04-17 17:04:42.942oai:tede2.pucrs.br:tede/1750Biblioteca Digital de Teses e Dissertaçõeshttp://tede2.pucrs.br/tede2/PRIhttps://tede2.pucrs.br/oai/requestbiblioteca.central@pucrs.br||opendoar:2015-04-17T20:04:42Biblioteca Digital de Teses e Dissertações da PUC_RS - Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS)false |
dc.title.por.fl_str_mv |
Efeito do plasma rico em plaquetas e da fibrina rica em plaquetas na cicatriza??o de feridas cut?neas em ratos |
title |
Efeito do plasma rico em plaquetas e da fibrina rica em plaquetas na cicatriza??o de feridas cut?neas em ratos |
spellingShingle |
Efeito do plasma rico em plaquetas e da fibrina rica em plaquetas na cicatriza??o de feridas cut?neas em ratos Camargo, Fernanda Ferreira de MEDICINA CICATRIZA??O DE FERIDAS PLASMAPLAQUETAS REGENERA??O TECIDUAL GUIADA CNPQ::CIENCIAS DA SAUDE::MEDICINA |
title_short |
Efeito do plasma rico em plaquetas e da fibrina rica em plaquetas na cicatriza??o de feridas cut?neas em ratos |
title_full |
Efeito do plasma rico em plaquetas e da fibrina rica em plaquetas na cicatriza??o de feridas cut?neas em ratos |
title_fullStr |
Efeito do plasma rico em plaquetas e da fibrina rica em plaquetas na cicatriza??o de feridas cut?neas em ratos |
title_full_unstemmed |
Efeito do plasma rico em plaquetas e da fibrina rica em plaquetas na cicatriza??o de feridas cut?neas em ratos |
title_sort |
Efeito do plasma rico em plaquetas e da fibrina rica em plaquetas na cicatriza??o de feridas cut?neas em ratos |
author |
Camargo, Fernanda Ferreira de |
author_facet |
Camargo, Fernanda Ferreira de |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Silva, Jefferson Luis Braga da |
dc.contributor.advisor1ID.fl_str_mv |
CPF:32077491000 |
dc.contributor.advisor1Lattes.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4707540D6 |
dc.contributor.authorID.fl_str_mv |
CPF:50978802004 |
dc.contributor.authorLattes.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4250720U8 |
dc.contributor.author.fl_str_mv |
Camargo, Fernanda Ferreira de |
contributor_str_mv |
Silva, Jefferson Luis Braga da |
dc.subject.por.fl_str_mv |
MEDICINA CICATRIZA??O DE FERIDAS PLASMAPLAQUETAS REGENERA??O TECIDUAL GUIADA |
topic |
MEDICINA CICATRIZA??O DE FERIDAS PLASMAPLAQUETAS REGENERA??O TECIDUAL GUIADA CNPQ::CIENCIAS DA SAUDE::MEDICINA |
dc.subject.cnpq.fl_str_mv |
CNPQ::CIENCIAS DA SAUDE::MEDICINA |
description |
Objective: To evaluate the effect of platelet-rich plasma (PRP) and platelet-rich fibrin (PRF) on cutaneous wound healing in rats by comparing treated and control wounds. Material and Methods: Forty-eight Wistar rats were used in the study. Two wounds with 1-cm diameter, of total skin thickness, were excised from the back of the animals and treated with either PRP or PRF and saline (paired model). The wounds were evaluated immediately after the procedure and after 7 and 14 days using macroscopic (photographs) and microscopic (histology) methods. The photographs were used to measure the area of wounds in order to analyse wound contraction (morphometric analysis). The histological images permitted a count-analysis of collagen concentration (histomorphometric analysis) while the histological slides providet the description of the wounds characteristics. Results: Morphometric analysis revealed statistically significant time effect (P<0.001) and treatment group effect (P=0.022) over the observation period. The rate of wound contraction was higher in PRF-treated wounds than in PRP-treated wounds (P=0.030). In the histomorphometric analysis, mean collagen concentration, on the images of the experimental groups, increased significantly (P<0.001) from day 7 to day 14. There was wide variation in the percentage of collagen concentration: 129% in the PRF group vs. 22% in the PRP group; however, this difference did not achieve statistical significance (P=0.380). Histology of the wounds suggested a more accelerated healing process in the PRF group vs. the PRP group, despite the lack of statistical significance in the characteristics analyzed. PRP-treated and PRF-treated groups were not statistically superior to their controls in any of the studied variables. Conclusion: The present results suggest that treatment with PRP and PRF were not more effective than saline for cutaneous wound healing in rats. However, PRF proved to be superior to PRP. |
publishDate |
2013 |
dc.date.available.fl_str_mv |
2013-11-11 |
dc.date.issued.fl_str_mv |
2013-08-08 |
dc.date.accessioned.fl_str_mv |
2015-04-14T13:35:49Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/masterThesis |
format |
masterThesis |
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publishedVersion |
dc.identifier.citation.fl_str_mv |
CAMARGO, Fernanda Ferreira de. Efeito do plasma rico em plaquetas e da fibrina rica em plaquetas na cicatriza??o de feridas cut?neas em ratos. 2013. 184 f. Disserta??o (Mestrado em Medicina e Ci?ncias da Sa?de) - Pontif?cia Universidade Cat?lica do Rio Grande do Sul, Porto Alegre, 2013. |
dc.identifier.uri.fl_str_mv |
http://tede2.pucrs.br/tede2/handle/tede/1750 |
identifier_str_mv |
CAMARGO, Fernanda Ferreira de. Efeito do plasma rico em plaquetas e da fibrina rica em plaquetas na cicatriza??o de feridas cut?neas em ratos. 2013. 184 f. Disserta??o (Mestrado em Medicina e Ci?ncias da Sa?de) - Pontif?cia Universidade Cat?lica do Rio Grande do Sul, Porto Alegre, 2013. |
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http://tede2.pucrs.br/tede2/handle/tede/1750 |
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Pontif?cia Universidade Cat?lica do Rio Grande do Sul |
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Programa de P?s-Gradua??o em Medicina e Ci?ncias da Sa?de |
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PUCRS |
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BR |
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Pontif?cia Universidade Cat?lica do Rio Grande do Sul |
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