Propaga??o e conserva??o in vitro de Martianthus leucocephalus (MART. ex BENTH.) J.F.B. PASTORE
Ano de defesa: | 2012 |
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Autor(a) principal: | |
Orientador(a): | |
Banca de defesa: | |
Tipo de documento: | Tese |
Tipo de acesso: | Acesso aberto |
Idioma: | por |
Instituição de defesa: |
Universidade Estadual de Feira de Santana
|
Programa de Pós-Graduação: |
Doutorado Acad?mico em Bot?nica
|
Departamento: |
DEPARTAMENTO DE CI?NCIAS BIOL?GICAS
|
País: |
Brasil
|
Palavras-chave em Português: | |
Palavras-chave em Inglês: | |
Área do conhecimento CNPq: | |
Link de acesso: | http://tede2.uefs.br:8080/handle/tede/1036 |
Resumo: | Martianthus leucocephalus (Mart. ex Benth.) J.F.B. Pastore (Lamiaceae) is an aromatic medicinal specie, endemic to the semiarid Northeast, has economic importance due to their pharmacological potential. This work aimed to study the in vitro propagation of the specie Martianthus leucocephalus developing a protocol for in vitro micropropagation and conservation, allowing the establishment of strategies for their conservation and sustainable. In vitro establishment were carried out: 1 - test of different culture media (MS, MS ?, WPM, Agar and Paper germtest) in vitro germination; 2 - test of different medium culture (MS, MS ? and WPM) on growth In vitro and 3 ? assessement of different volumes of culture medium (in 500mL flasks) on in vitro growth of M. leucocephalus. In the process of morphogenesis in vitro, test: 1 - different types of explants (leaf segment, segment internodal and nodal) associated with different cytokinins (BAP, KIN and TDZ) 2 - combination of different concentrations of BAP and NAA; 3 - different explants at this position as the starting material, 4 - carbon sources (sucrose, maltose, fructose, lactose and glucose); 5 - organogenic potential from petioles sheets subjected to different cytokinins (KIN, BAP , TDZ and ZEA). For acclimatization, the plants have undergone a hardening process, which were used in various closures for tubes. In the greenhouse, plants were transferred to plastic cups containing potting soil, being covered with type pet bottles with lids, three days after the covers were desenroscadas, taken in the tenth and twenty-first day the bottles were removed completely. For the experiment of induction of somatic embryogenesis, we used segments of leaves under different concentrations of 2,4-D and KIN was determined from the growth curve of the fresh callus until the 90th day of culture at intervals of nine days. Carbohydrates were identified and quantified by HPLC. For histological studies, samples were fixed 70% FAA and prepared for analysis by optical microscope, with embedment in historesin. Histological sections were stained with toluidine blue (1%). For in vitro conservation Two experiments were conducted: 1 - we used the plant growth: ancymidol or paclobutrazol (0.0, 0.85, 1.7, 3.4 and 6.8 ?M) and 2 - was evaluated different agents osmotic (sucrose - Sac, mannitol - Man and sorbitol - Sor) and different concentrations (87.64, 131.46, 176.28, 219.10 and 262.92 mM). Osmotic agents mannitol and sorbitol were used in combination with sucrose. The in vitro germination of M. leucocephalus should be performed in culture medium MS?; chemical sterilization is an effective method for the in vitro culture of two species object of study of this work, the MS medium is the most suitable for the in vitro culture of M. leucocephalus and can be used is 60mL of medium in 500 ml flasks. Only the nodal segment showed morphogenetic response, and cytokinins promoted low rate of multiplication. The multiplication of shoots can be performed with 1.34 ?M of ANA from nodal segment, sucrose should be maintained as the best carbon source for the morphogenesis of M. leucocephalus, since the alternative carbon sources according to variables not outweigh sucrose as the multiplication of shoots. Organogenesis from leaf petiole with this potential organogenic shown, but it is necessary to test other culture media to favor higher regeneration of explants. The seedlings can be acclimatized without having to go through the process of hardening. Embryogenic calli were induced with 1?M 2,4-D + 0.5 ?M KIN, it was possible to identify the five stages of callus growth during the incubation period of embryogenic calli identified three carbohydrates: glucose, fructose and sucrose . At nine days incubation verified the presence of globular embryos whose ontogeny occurred from the vascular system and detected the greatest glucose content at 9 and 18 days of incubation. The paclobutrazol and ancymidol promoted decrease in plant growth, however the rate of survival at 240 days was below the results obtained with the osmotic agents. The conservation in vitro M. leucocephalus can be carried out in culture medium supplemented with Sac + 43.82 43.82 mM mM mM Man or Sac + 65.73 65.73 mM Man, since these treatments promoted survival rate of approximately 80%, while reducing the length of shoots, number of leaves, number of senescent leaves and promoted gain in dry matter of the shoot. |
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Santana, Jos? Raniere Ferreira de90725549572http://lattes.cnpq.br/8815264894426999Nepomuceno, Cristina Ferreira2020-04-08T21:48:46Z2012-08-31NEPOMUCENO, Cristina Ferreira. Propaga??o e conserva??o in vitro de Martianthus leucocephalus (MART. ex BENTH.) J.F.B. PASTORE. 2012. 179 f. Tese (Doutorado Acad?mico em Bot?nica)- Universidade Estadual de Feira de Santana, Feira de Santana, 2012.http://tede2.uefs.br:8080/handle/tede/1036Martianthus leucocephalus (Mart. ex Benth.) J.F.B. Pastore (Lamiaceae) is an aromatic medicinal specie, endemic to the semiarid Northeast, has economic importance due to their pharmacological potential. This work aimed to study the in vitro propagation of the specie Martianthus leucocephalus developing a protocol for in vitro micropropagation and conservation, allowing the establishment of strategies for their conservation and sustainable. In vitro establishment were carried out: 1 - test of different culture media (MS, MS ?, WPM, Agar and Paper germtest) in vitro germination; 2 - test of different medium culture (MS, MS ? and WPM) on growth In vitro and 3 ? assessement of different volumes of culture medium (in 500mL flasks) on in vitro growth of M. leucocephalus. In the process of morphogenesis in vitro, test: 1 - different types of explants (leaf segment, segment internodal and nodal) associated with different cytokinins (BAP, KIN and TDZ) 2 - combination of different concentrations of BAP and NAA; 3 - different explants at this position as the starting material, 4 - carbon sources (sucrose, maltose, fructose, lactose and glucose); 5 - organogenic potential from petioles sheets subjected to different cytokinins (KIN, BAP , TDZ and ZEA). For acclimatization, the plants have undergone a hardening process, which were used in various closures for tubes. In the greenhouse, plants were transferred to plastic cups containing potting soil, being covered with type pet bottles with lids, three days after the covers were desenroscadas, taken in the tenth and twenty-first day the bottles were removed completely. For the experiment of induction of somatic embryogenesis, we used segments of leaves under different concentrations of 2,4-D and KIN was determined from the growth curve of the fresh callus until the 90th day of culture at intervals of nine days. Carbohydrates were identified and quantified by HPLC. For histological studies, samples were fixed 70% FAA and prepared for analysis by optical microscope, with embedment in historesin. Histological sections were stained with toluidine blue (1%). For in vitro conservation Two experiments were conducted: 1 - we used the plant growth: ancymidol or paclobutrazol (0.0, 0.85, 1.7, 3.4 and 6.8 ?M) and 2 - was evaluated different agents osmotic (sucrose - Sac, mannitol - Man and sorbitol - Sor) and different concentrations (87.64, 131.46, 176.28, 219.10 and 262.92 mM). Osmotic agents mannitol and sorbitol were used in combination with sucrose. The in vitro germination of M. leucocephalus should be performed in culture medium MS?; chemical sterilization is an effective method for the in vitro culture of two species object of study of this work, the MS medium is the most suitable for the in vitro culture of M. leucocephalus and can be used is 60mL of medium in 500 ml flasks. Only the nodal segment showed morphogenetic response, and cytokinins promoted low rate of multiplication. The multiplication of shoots can be performed with 1.34 ?M of ANA from nodal segment, sucrose should be maintained as the best carbon source for the morphogenesis of M. leucocephalus, since the alternative carbon sources according to variables not outweigh sucrose as the multiplication of shoots. Organogenesis from leaf petiole with this potential organogenic shown, but it is necessary to test other culture media to favor higher regeneration of explants. The seedlings can be acclimatized without having to go through the process of hardening. Embryogenic calli were induced with 1?M 2,4-D + 0.5 ?M KIN, it was possible to identify the five stages of callus growth during the incubation period of embryogenic calli identified three carbohydrates: glucose, fructose and sucrose . At nine days incubation verified the presence of globular embryos whose ontogeny occurred from the vascular system and detected the greatest glucose content at 9 and 18 days of incubation. The paclobutrazol and ancymidol promoted decrease in plant growth, however the rate of survival at 240 days was below the results obtained with the osmotic agents. The conservation in vitro M. leucocephalus can be carried out in culture medium supplemented with Sac + 43.82 43.82 mM mM mM Man or Sac + 65.73 65.73 mM Man, since these treatments promoted survival rate of approximately 80%, while reducing the length of shoots, number of leaves, number of senescent leaves and promoted gain in dry matter of the shoot.Martianthus leucocephalus (Mart. ex Benth.) J.F.B. Pastore (Lamiaceae) ? uma esp?cie medicinal arom?tica, end?mica do semi?rido nordestino, possui import?ncia econ?mica devido ao seu potencial farmacol?gico. Este trabalho teve como objetivo estudar a propaga??o in vitro da esp?cie Martianthus leucocephalus desenvolvendo um protocolo de micropropaga??o e conserva??o in vitro, permitindo o estabelecimento de estrat?gias para a sua preserva??o e explora??o sustent?vel. No estabelecimento in vitro foram testados: 1 ? testou-se diferentes meios de cultura (MS, MS?, WPM, Agar e Papel germtest) na germina??o in vitro; 2 - testou-se diferentes meio cultura (MS, MS? e WPM) no crescimento in vitro e 3 ? avaliou-se diferentes volumes de meio de cultura (em frascos de 500mL) no crescimento in vitro de M. leucocephalus. No processo de morfog?nese in vitro, testou-se: 1 - diferentes tipos de explantes (segmento de folha, segmento internodal e nodal) associado a diferentes citocininas (BAP, KIN e TDZ); 2 - combina??o de diferentes concentra??es de BAP e ANA; 3 - diferentes explantes quanto a posi??o deste no material de partida; 4 - diferentes fontes de carbono (sacarose, maltose, frutose, lactose e glicose); 5 - o potencial organog?nico a partir de folhas com pec?olos submetidas a diferentes citocininas (KIN, BAP, TDZ e ZEA). Para aclimatiza??o, as plantas passaram por um processo de rustifica??o, em que foram utilizados diferentes fechamentos dos tubos de ensaio. Na casa de vegeta??o as plantas foram transferidas para copos pl?sticos, contendo terra vegetal, sendo cobertas com garrafas tipo pet com tampa, depois de tr?s dias as tampas foram desenroscadas, no d?cimo retiradas e no vig?simo primeiro dia as garrafas foram retiradas totalmente. Para o experimento de indu??o de calos embriog?nicos, utilizou-se segmentos de folhas sob diferentes concentra??es de 2,4-D e KIN, foi determinada a curva de crescimento a partir da mat?ria fresca dos calos at? o 90? dia de cultivo, em intervalos de nove dias. Os carboidratos foram identificados e quantificados em HPLC. Para os estudos histol?gicos, as amostras foram fixadas FAA 70% e preparadas para an?lise em microsc?pia ?ptica, com emblocamento em historesina. As sec??es histol?gicas foram coradas com azul de toluidina (1%). Para conserva??o in vitro foram realizados dois experimentos: 1 ? utilizou-se os retardantes vegetais: ancimidol ou paclobutrazol (0,0; 0,85; 1,7; 3,4 e 6,8?M) e 2 ? avaliou-se diferentes agentes osm?ticos (sacarose - Sac, manitol - Man e sorbitol - Sor) e diferentes concentra??es (87,64; 131,46; 176,28; 219,10 e 262,92mM). Os agentes osm?ticos manitol e sorbitol foram utilizados combinados com a sacarose. A germina??o in vitro de M. leucocephalus deve ser realizada em meio de cultura MS?; a esteriliza??o qu?mica ? um m?todo eficiente para o cultivo in vitro das duas esp?cies objeto de estudo desse trabalho; o meio de cultura MS ? o mais indicado para o cultivo in vitro de M. leucocephalus e pode ser utilizado ? 60mL de meio em frascos de 500mL. Apenas o segmento nodal apresentou resposta morfogen?tica, sendo que as citocininas promoveram baixa taxa de multiplica??o. A multiplica??o dos brotos pode ser realizada com 1,34?M de ANA, a partir de segmento nodal, a sacarose deve ser mantida como a melhor fonte de carbono para a morfog?nese de M. leucocephalus, visto que, as fontes de carbonos alternativas de acordo as vari?veis analisadas n?o superam a sacarose quanto ? multiplica??o dos brotos. Na organog?nese a partir de folha com pec?olo, este mostrou potencial organog?nico, por?m ? necess?rio testar outros meios de cultura de forma a favorecer maior regenera??o dos explantes. As mudas obtidas podem ser aclimatizadas sem a necessidade de passar pelo processo de rustifica??o. Calos embriog?nicos foram induzidos com 1?M de 2,4-D + 0,5?M de KIN, foi poss?vel identificar as cinco fases de crescimento dos calos, durante o per?odo de incuba??o dos calos embriog?nicos, identificou-se tr?s carboidratos: glicose, frutose e sacarose. Aos noves dias de incuba??o verificou-se a presen?a de embri?es globulares, cuja ontogenia ocorreu a partir do sistema vascular e detectou-se o maior teor de glicose aos 9 e 18 dias de incuba??o. O ancimidol e o paclobutrazol promoveram decr?scimos no crescimento das plantas, contudo a taxa de sobreviv?ncia aos 240 dias foi inferior aos resultados obtidos com os agentes osm?ticos. A conserva??o in vitro de M. leucocephalus pode ser realizada em meio de cultura suplementado com 43,82mM Sac + 43,82mM Man ou 65,73mM Sac + 65,73mM Man, uma vez que estes tratamentos promoveram taxa de sobreviv?ncia em torno de 80%, al?m de reduzir o comprimento de parte a?rea, n?mero de folhas, n?mero de folhas senescentes e promoveu ganho na mat?ria seca de parte a?rea.Submitted by Ricardo Cedraz Duque Moliterno (ricardo.moliterno@uefs.br) on 2020-04-08T21:48:46Z No. of bitstreams: 1 Tese_Cristina Ferreira.pdf: 6063119 bytes, checksum: 4f406c653f56d5a9a2b44960d5bbab34 (MD5)Made available in DSpace on 2020-04-08T21:48:46Z (GMT). No. of bitstreams: 1 Tese_Cristina Ferreira.pdf: 6063119 bytes, checksum: 4f406c653f56d5a9a2b44960d5bbab34 (MD5) Previous issue date: 2012-08-31Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior - CAPESapplication/pdfporUniversidade Estadual de Feira de SantanaDoutorado Acad?mico em Bot?nicaUEFSBrasilDEPARTAMENTO DE CI?NCIAS BIOL?GICASLamiaceaeEmbriog?nese som?ticaCultura de tecidosPlanta medicinalMicropropaga??oSomatic embryogenesisFabric cultureMedicinal plantMicropropagationBOTANICA::FISIOLOGIA VEGETALPropaga??o e conserva??o in vitro de Martianthus leucocephalus (MART. ex BENTH.) J.F.B. 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dc.title.por.fl_str_mv |
Propaga??o e conserva??o in vitro de Martianthus leucocephalus (MART. ex BENTH.) J.F.B. PASTORE |
title |
Propaga??o e conserva??o in vitro de Martianthus leucocephalus (MART. ex BENTH.) J.F.B. PASTORE |
spellingShingle |
Propaga??o e conserva??o in vitro de Martianthus leucocephalus (MART. ex BENTH.) J.F.B. PASTORE Nepomuceno, Cristina Ferreira Lamiaceae Embriog?nese som?tica Cultura de tecidos Planta medicinal Micropropaga??o Somatic embryogenesis Fabric culture Medicinal plant Micropropagation BOTANICA::FISIOLOGIA VEGETAL |
title_short |
Propaga??o e conserva??o in vitro de Martianthus leucocephalus (MART. ex BENTH.) J.F.B. PASTORE |
title_full |
Propaga??o e conserva??o in vitro de Martianthus leucocephalus (MART. ex BENTH.) J.F.B. PASTORE |
title_fullStr |
Propaga??o e conserva??o in vitro de Martianthus leucocephalus (MART. ex BENTH.) J.F.B. PASTORE |
title_full_unstemmed |
Propaga??o e conserva??o in vitro de Martianthus leucocephalus (MART. ex BENTH.) J.F.B. PASTORE |
title_sort |
Propaga??o e conserva??o in vitro de Martianthus leucocephalus (MART. ex BENTH.) J.F.B. PASTORE |
author |
Nepomuceno, Cristina Ferreira |
author_facet |
Nepomuceno, Cristina Ferreira |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Santana, Jos? Raniere Ferreira de |
dc.contributor.authorID.fl_str_mv |
90725549572 |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/8815264894426999 |
dc.contributor.author.fl_str_mv |
Nepomuceno, Cristina Ferreira |
contributor_str_mv |
Santana, Jos? Raniere Ferreira de |
dc.subject.por.fl_str_mv |
Lamiaceae Embriog?nese som?tica Cultura de tecidos Planta medicinal Micropropaga??o |
topic |
Lamiaceae Embriog?nese som?tica Cultura de tecidos Planta medicinal Micropropaga??o Somatic embryogenesis Fabric culture Medicinal plant Micropropagation BOTANICA::FISIOLOGIA VEGETAL |
dc.subject.eng.fl_str_mv |
Somatic embryogenesis Fabric culture Medicinal plant Micropropagation |
dc.subject.cnpq.fl_str_mv |
BOTANICA::FISIOLOGIA VEGETAL |
description |
Martianthus leucocephalus (Mart. ex Benth.) J.F.B. Pastore (Lamiaceae) is an aromatic medicinal specie, endemic to the semiarid Northeast, has economic importance due to their pharmacological potential. This work aimed to study the in vitro propagation of the specie Martianthus leucocephalus developing a protocol for in vitro micropropagation and conservation, allowing the establishment of strategies for their conservation and sustainable. In vitro establishment were carried out: 1 - test of different culture media (MS, MS ?, WPM, Agar and Paper germtest) in vitro germination; 2 - test of different medium culture (MS, MS ? and WPM) on growth In vitro and 3 ? assessement of different volumes of culture medium (in 500mL flasks) on in vitro growth of M. leucocephalus. In the process of morphogenesis in vitro, test: 1 - different types of explants (leaf segment, segment internodal and nodal) associated with different cytokinins (BAP, KIN and TDZ) 2 - combination of different concentrations of BAP and NAA; 3 - different explants at this position as the starting material, 4 - carbon sources (sucrose, maltose, fructose, lactose and glucose); 5 - organogenic potential from petioles sheets subjected to different cytokinins (KIN, BAP , TDZ and ZEA). For acclimatization, the plants have undergone a hardening process, which were used in various closures for tubes. In the greenhouse, plants were transferred to plastic cups containing potting soil, being covered with type pet bottles with lids, three days after the covers were desenroscadas, taken in the tenth and twenty-first day the bottles were removed completely. For the experiment of induction of somatic embryogenesis, we used segments of leaves under different concentrations of 2,4-D and KIN was determined from the growth curve of the fresh callus until the 90th day of culture at intervals of nine days. Carbohydrates were identified and quantified by HPLC. For histological studies, samples were fixed 70% FAA and prepared for analysis by optical microscope, with embedment in historesin. Histological sections were stained with toluidine blue (1%). For in vitro conservation Two experiments were conducted: 1 - we used the plant growth: ancymidol or paclobutrazol (0.0, 0.85, 1.7, 3.4 and 6.8 ?M) and 2 - was evaluated different agents osmotic (sucrose - Sac, mannitol - Man and sorbitol - Sor) and different concentrations (87.64, 131.46, 176.28, 219.10 and 262.92 mM). Osmotic agents mannitol and sorbitol were used in combination with sucrose. The in vitro germination of M. leucocephalus should be performed in culture medium MS?; chemical sterilization is an effective method for the in vitro culture of two species object of study of this work, the MS medium is the most suitable for the in vitro culture of M. leucocephalus and can be used is 60mL of medium in 500 ml flasks. Only the nodal segment showed morphogenetic response, and cytokinins promoted low rate of multiplication. The multiplication of shoots can be performed with 1.34 ?M of ANA from nodal segment, sucrose should be maintained as the best carbon source for the morphogenesis of M. leucocephalus, since the alternative carbon sources according to variables not outweigh sucrose as the multiplication of shoots. Organogenesis from leaf petiole with this potential organogenic shown, but it is necessary to test other culture media to favor higher regeneration of explants. The seedlings can be acclimatized without having to go through the process of hardening. Embryogenic calli were induced with 1?M 2,4-D + 0.5 ?M KIN, it was possible to identify the five stages of callus growth during the incubation period of embryogenic calli identified three carbohydrates: glucose, fructose and sucrose . At nine days incubation verified the presence of globular embryos whose ontogeny occurred from the vascular system and detected the greatest glucose content at 9 and 18 days of incubation. The paclobutrazol and ancymidol promoted decrease in plant growth, however the rate of survival at 240 days was below the results obtained with the osmotic agents. The conservation in vitro M. leucocephalus can be carried out in culture medium supplemented with Sac + 43.82 43.82 mM mM mM Man or Sac + 65.73 65.73 mM Man, since these treatments promoted survival rate of approximately 80%, while reducing the length of shoots, number of leaves, number of senescent leaves and promoted gain in dry matter of the shoot. |
publishDate |
2012 |
dc.date.issued.fl_str_mv |
2012-08-31 |
dc.date.accessioned.fl_str_mv |
2020-04-08T21:48:46Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/doctoralThesis |
format |
doctoralThesis |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
NEPOMUCENO, Cristina Ferreira. Propaga??o e conserva??o in vitro de Martianthus leucocephalus (MART. ex BENTH.) J.F.B. PASTORE. 2012. 179 f. Tese (Doutorado Acad?mico em Bot?nica)- Universidade Estadual de Feira de Santana, Feira de Santana, 2012. |
dc.identifier.uri.fl_str_mv |
http://tede2.uefs.br:8080/handle/tede/1036 |
identifier_str_mv |
NEPOMUCENO, Cristina Ferreira. Propaga??o e conserva??o in vitro de Martianthus leucocephalus (MART. ex BENTH.) J.F.B. PASTORE. 2012. 179 f. Tese (Doutorado Acad?mico em Bot?nica)- Universidade Estadual de Feira de Santana, Feira de Santana, 2012. |
url |
http://tede2.uefs.br:8080/handle/tede/1036 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.program.fl_str_mv |
-4908229543721086191 |
dc.relation.confidence.fl_str_mv |
600 600 600 600 |
dc.relation.department.fl_str_mv |
5026123383450589282 |
dc.relation.cnpq.fl_str_mv |
-289993050269505198 |
dc.relation.sponsorship.fl_str_mv |
3590462550136975366 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Estadual de Feira de Santana |
dc.publisher.program.fl_str_mv |
Doutorado Acad?mico em Bot?nica |
dc.publisher.initials.fl_str_mv |
UEFS |
dc.publisher.country.fl_str_mv |
Brasil |
dc.publisher.department.fl_str_mv |
DEPARTAMENTO DE CI?NCIAS BIOL?GICAS |
publisher.none.fl_str_mv |
Universidade Estadual de Feira de Santana |
dc.source.none.fl_str_mv |
reponame:Biblioteca Digital de Teses e Dissertações da UEFS instname:Universidade Estadual de Feira de Santana (UEFS) instacron:UEFS |
instname_str |
Universidade Estadual de Feira de Santana (UEFS) |
instacron_str |
UEFS |
institution |
UEFS |
reponame_str |
Biblioteca Digital de Teses e Dissertações da UEFS |
collection |
Biblioteca Digital de Teses e Dissertações da UEFS |
bitstream.url.fl_str_mv |
http://tede2.uefs.br:8080/bitstream/tede/1036/2/Tese_Cristina+Ferreira.pdf http://tede2.uefs.br:8080/bitstream/tede/1036/1/license.txt |
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bitstream.checksumAlgorithm.fl_str_mv |
MD5 MD5 |
repository.name.fl_str_mv |
Biblioteca Digital de Teses e Dissertações da UEFS - Universidade Estadual de Feira de Santana (UEFS) |
repository.mail.fl_str_mv |
bcuefs@uefs.br|| bcref@uefs.br||bcuefs@uefs.br |
_version_ |
1796793640916353024 |