Taxonomia polif?sica de bact?rias diazotr?ficas do g?nero Sphingomonas spp. e efeito da inocula??o em plantas de arroz
Ano de defesa: | 2008 |
---|---|
Autor(a) principal: | |
Orientador(a): | |
Banca de defesa: | |
Tipo de documento: | Dissertação |
Tipo de acesso: | Acesso aberto |
Idioma: | por |
Instituição de defesa: |
Universidade Federal Rural do Rio de Janeiro
|
Programa de Pós-Graduação: |
Programa de P?s-Gradua??o em Agronomia - Ci?ncia do Solo
|
Departamento: |
Instituto de Agronomia
|
País: |
Brasil
|
Palavras-chave em Português: | |
Palavras-chave em Inglês: | |
Área do conhecimento CNPq: | |
Link de acesso: | https://tede.ufrrj.br/jspui/handle/tede/270 |
Resumo: | Rice (Oryza sativa L.) is a widely consumed food on the planet, suppling more than 50% of the world's food. Approximately 150 million hectares are used for its cultivation, and of these, 32% grow in upland conditions. Rice is usually grown in N-deficient soils, demanding that the element be supplied to the field by commercially available N fertilizers. Unfortunately, a substantial amount of the N applied as fertilizers is lost through different mechanisms, causing environmental pollution and economical problems. Utilization of biological N2 fixation (BNF) technology may decrease the application of N fertilizers, reducing environmental and economical risks. Among the wide variety of bacteria that are associated with rice plants, Sphingomonas spp. are often found; although the first nitrogen-fixing specie has been reported only recently. Due the large number of Sphingomonas spp. obtained from rice plants grown in the experimental field of Embrapa Agrobiologia, this study aimed to study the genetic diversity and identify these isolates through molecular, physiological and quimiotaxonomic methods, as well as assess the behavior of the population and the efficiency of some isolated in growth-promoting of upland condition rice plants cultivated in a greenhouse. The results showed that only 50% of the isolates presented in vitro nitrogenase activity and the gene related to BNF. All diazotrophic isolates were identified as Sphingomonas spp., showing little genetic diversity among themselves and greater similarity to Sphingomonas azotifigens. The population of diazotrophic bacteria showed great numbers present in the rice roots. There was an increase in grain yield of rice by 48% when inoculated with some strains of Sphingomonas spp. and Herbaspirillum seropedicae (ZAE94) on the treatment without inoculation and without nitrogen. |
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Baldani, Vera L?cia DivanCPF: 443.249.287-20http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4788918P8Ara?jo, Jean Luiz Sim?es deCPF: 728.000.464-49http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4793441D6CPF: 092.655.887-05http://lattes.cnpq.br/1627896730005167Videira, Sandy Sampaio2016-04-26T19:36:55Z2009-09-142008-02-27VIDEIRA, Sandy Sampaio. Taxonomia polif?sica de bact?rias diazotr?ficas do g?nero Sphingomonas spp. e efeito da inocula??o em plantas de arroz. 2008. 140 f. Disserta??o (Mestrado em Agronomia, Ci?ncia do Solo) - Instituto de Agronomia,Universidade Federal Rural do Rio de Janeiro, Serop?dica - RJ, 2008.https://tede.ufrrj.br/jspui/handle/tede/270Rice (Oryza sativa L.) is a widely consumed food on the planet, suppling more than 50% of the world's food. Approximately 150 million hectares are used for its cultivation, and of these, 32% grow in upland conditions. Rice is usually grown in N-deficient soils, demanding that the element be supplied to the field by commercially available N fertilizers. Unfortunately, a substantial amount of the N applied as fertilizers is lost through different mechanisms, causing environmental pollution and economical problems. Utilization of biological N2 fixation (BNF) technology may decrease the application of N fertilizers, reducing environmental and economical risks. Among the wide variety of bacteria that are associated with rice plants, Sphingomonas spp. are often found; although the first nitrogen-fixing specie has been reported only recently. Due the large number of Sphingomonas spp. obtained from rice plants grown in the experimental field of Embrapa Agrobiologia, this study aimed to study the genetic diversity and identify these isolates through molecular, physiological and quimiotaxonomic methods, as well as assess the behavior of the population and the efficiency of some isolated in growth-promoting of upland condition rice plants cultivated in a greenhouse. The results showed that only 50% of the isolates presented in vitro nitrogenase activity and the gene related to BNF. All diazotrophic isolates were identified as Sphingomonas spp., showing little genetic diversity among themselves and greater similarity to Sphingomonas azotifigens. The population of diazotrophic bacteria showed great numbers present in the rice roots. There was an increase in grain yield of rice by 48% when inoculated with some strains of Sphingomonas spp. and Herbaspirillum seropedicae (ZAE94) on the treatment without inoculation and without nitrogen.O arroz (Oryza sativa L.) ? um alimento extensamente consumido no planeta, suprindo mais de 50% da alimenta??o mundial. Aproximadamente 150 milh?es de hectares s?o utilizados para seu cultivo, e destes, 32% crescem em condi??es de terras altas. O arroz ? normalmente cultivado em solos com defici?ncia de N, exigindo que este elemento seja fornecido por meio de fertilizantes nitrogenados comercialmente dispon?veis. Infelizmente, uma quantidade significativa do N aplicado como fertilizantes perde-se atrav?s de diferentes mecanismos, causando problemas de polui??o ambiental e aumento no custo de produ??o. A utiliza??o da tecnologia de fixa??o biol?gica de N2 (FBN) pode diminuir a aplica??o de N-fertilizantes, reduzindo os elevados custos econ?micos e ambientais. Dentre a grande diversidade de bact?rias que se associam com plantas de arroz, bact?rias do g?nero Sphingomonas s?o frequentemente encontradas, entretanto a primeira esp?cie fixadora de nitrog?nio s? foi descrita recentemente. Devido ao grande n?mero de bact?rias deste g?nero obtidas de plantas de arroz cultivadas no campo experimental da Embrapa Agrobiologia, o presente estudo visou estudar a diversidade gen?tica e identificar estes isolados, atrav?s de m?todos moleculares, fisiol?gicos e quimiotaxon?micos, assim como avaliar o comportamento populacional e a efici?ncia de alguns isolados na promo??o de crescimento vegetal de plantas de arroz de terras altas cultivadas em casa de vegeta??o. Os resultados obtidos mostraram que somente 50% dos isolados apresentaram atividade da nitrogenase in vitro e o gene relacionado a FBN. Todos os isolados diazotr?ficos foram identificados como Sphingomonas spp., apresentando pouca diversidade gen?tica entre si e maior similaridade com a esp?cie Sphingomonas azotifigens. A popula??o de bact?rias diazotr?ficas revelou maior n?mero nas ra?zes. Verificou-se um incremento no rendimento de gr?os do arroz de at? 48% quando inoculado com algumas estirpes de Sphingomonas spp. e Herbaspirillum seropedicae (ZAE94) sobre o tratamento sem inocula??o e sem aduba??o nitrogenada.Made available in DSpace on 2016-04-26T19:36:55Z (GMT). No. of bitstreams: 1 2008 - Sandy Sampaio Videira.pdf: 4433575 bytes, checksum: 8970bad6b98de1356e8201e44cb868fa (MD5) Previous issue date: 2008-02-27FAPERJ - Funda??o Carlos Chagas Filho de Amparo ? Pesquisa do Estado do Rio de Janeiroapplication/pdfhttps://tede.ufrrj.br/retrieve/3814/2008%20-%20Sandy%20Sampaio%20Videira.pdf.jpghttps://tede.ufrrj.br/retrieve/18064/2008%20-%20Sandy%20Sampaio%20Videira.pdf.jpghttps://tede.ufrrj.br/retrieve/24403/2008%20-%20Sandy%20Sampaio%20Videira.pdf.jpghttps://tede.ufrrj.br/retrieve/30751/2008%20-%20Sandy%20Sampaio%20Videira.pdf.jpghttps://tede.ufrrj.br/retrieve/37131/2008%20-%20Sandy%20Sampaio%20Videira.pdf.jpghttps://tede.ufrrj.br/retrieve/43521/2008%20-%20Sandy%20Sampaio%20Videira.pdf.jpghttps://tede.ufrrj.br/retrieve/49923/2008%20-%20Sandy%20Sampaio%20Videira.pdf.jpghttps://tede.ufrrj.br/retrieve/56344/2008%20-%20Sandy%20Sampaio%20Videira.pdf.jpgporUniversidade Federal Rural do Rio de JaneiroPrograma de P?s-Gradua??o em Agronomia - Ci?ncia do SoloUFRRJBrasilInstituto de Agronomiafixa??o biol?gica de nitrog?nionitrogenaseOryza sativabiological nitrogen fixationnitrogenaseAgronomiaTaxonomia polif?sica de bact?rias diazotr?ficas do g?nero Sphingomonas spp. e efeito da inocula??o em plantas de arrozPoliphasic taxonomy of Sphingomonas spp. diazotrophic bacteria and effect of inoculation in rice plantsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisinfo:eu-repo/semantics/openAccessreponame:Biblioteca Digital de Teses e Dissertações da UFRRJinstname:Universidade Federal Rural do Rio de Janeiro (UFRRJ)instacron:UFRRJTEXT2008 - Sandy Sampaio Videira.pdf.txt2008 - Sandy Sampaio Videira.pdf.txttext/plain340236http://localhost:8080/tede/bitstream/tede/270/16/2008+-+Sandy+Sampaio+Videira.pdf.txta0af76f716d8e49c36a79f77ddcc2de7MD516ORIGINAL2008 - Sandy Sampaio Videira.pdf2008 - Sandy Sampaio Videira.pdfapplication/pdf4440597http://localhost:8080/tede/bitstream/tede/270/3/2008+-+Sandy+Sampaio+Videira.pdf9362fb7b9fe93b2812b762af28e177b3MD53THUMBNAIL2008 - Sandy Sampaio Videira.pdf.jpg2008 - Sandy Sampaio Videira.pdf.jpgimage/jpeg1943http://localhost:8080/tede/bitstream/tede/270/17/2008+-+Sandy+Sampaio+Videira.pdf.jpgcc73c4c239a4c332d642ba1e7c7a9fb2MD517tede/2702022-02-14 21:29:26.643oai:localhost:tede/270Biblioteca Digital de Teses e Dissertaçõeshttps://tede.ufrrj.br/PUBhttps://tede.ufrrj.br/oai/requestbibliot@ufrrj.br||bibliot@ufrrj.bropendoar:2022-02-14T23:29:26Biblioteca Digital de Teses e Dissertações da UFRRJ - Universidade Federal Rural do Rio de Janeiro (UFRRJ)false |
dc.title.por.fl_str_mv |
Taxonomia polif?sica de bact?rias diazotr?ficas do g?nero Sphingomonas spp. e efeito da inocula??o em plantas de arroz |
dc.title.alternative.eng.fl_str_mv |
Poliphasic taxonomy of Sphingomonas spp. diazotrophic bacteria and effect of inoculation in rice plants |
title |
Taxonomia polif?sica de bact?rias diazotr?ficas do g?nero Sphingomonas spp. e efeito da inocula??o em plantas de arroz |
spellingShingle |
Taxonomia polif?sica de bact?rias diazotr?ficas do g?nero Sphingomonas spp. e efeito da inocula??o em plantas de arroz Videira, Sandy Sampaio fixa??o biol?gica de nitrog?nio nitrogenase Oryza sativa biological nitrogen fixation nitrogenase Agronomia |
title_short |
Taxonomia polif?sica de bact?rias diazotr?ficas do g?nero Sphingomonas spp. e efeito da inocula??o em plantas de arroz |
title_full |
Taxonomia polif?sica de bact?rias diazotr?ficas do g?nero Sphingomonas spp. e efeito da inocula??o em plantas de arroz |
title_fullStr |
Taxonomia polif?sica de bact?rias diazotr?ficas do g?nero Sphingomonas spp. e efeito da inocula??o em plantas de arroz |
title_full_unstemmed |
Taxonomia polif?sica de bact?rias diazotr?ficas do g?nero Sphingomonas spp. e efeito da inocula??o em plantas de arroz |
title_sort |
Taxonomia polif?sica de bact?rias diazotr?ficas do g?nero Sphingomonas spp. e efeito da inocula??o em plantas de arroz |
author |
Videira, Sandy Sampaio |
author_facet |
Videira, Sandy Sampaio |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Baldani, Vera L?cia Divan |
dc.contributor.advisor1ID.fl_str_mv |
CPF: 443.249.287-20 |
dc.contributor.advisor1Lattes.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4788918P8 |
dc.contributor.advisor-co1.fl_str_mv |
Ara?jo, Jean Luiz Sim?es de |
dc.contributor.advisor-co1ID.fl_str_mv |
CPF: 728.000.464-49 |
dc.contributor.advisor-co1Lattes.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4793441D6 |
dc.contributor.authorID.fl_str_mv |
CPF: 092.655.887-05 |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/1627896730005167 |
dc.contributor.author.fl_str_mv |
Videira, Sandy Sampaio |
contributor_str_mv |
Baldani, Vera L?cia Divan Ara?jo, Jean Luiz Sim?es de |
dc.subject.por.fl_str_mv |
fixa??o biol?gica de nitrog?nio nitrogenase Oryza sativa |
topic |
fixa??o biol?gica de nitrog?nio nitrogenase Oryza sativa biological nitrogen fixation nitrogenase Agronomia |
dc.subject.eng.fl_str_mv |
biological nitrogen fixation nitrogenase |
dc.subject.cnpq.fl_str_mv |
Agronomia |
description |
Rice (Oryza sativa L.) is a widely consumed food on the planet, suppling more than 50% of the world's food. Approximately 150 million hectares are used for its cultivation, and of these, 32% grow in upland conditions. Rice is usually grown in N-deficient soils, demanding that the element be supplied to the field by commercially available N fertilizers. Unfortunately, a substantial amount of the N applied as fertilizers is lost through different mechanisms, causing environmental pollution and economical problems. Utilization of biological N2 fixation (BNF) technology may decrease the application of N fertilizers, reducing environmental and economical risks. Among the wide variety of bacteria that are associated with rice plants, Sphingomonas spp. are often found; although the first nitrogen-fixing specie has been reported only recently. Due the large number of Sphingomonas spp. obtained from rice plants grown in the experimental field of Embrapa Agrobiologia, this study aimed to study the genetic diversity and identify these isolates through molecular, physiological and quimiotaxonomic methods, as well as assess the behavior of the population and the efficiency of some isolated in growth-promoting of upland condition rice plants cultivated in a greenhouse. The results showed that only 50% of the isolates presented in vitro nitrogenase activity and the gene related to BNF. All diazotrophic isolates were identified as Sphingomonas spp., showing little genetic diversity among themselves and greater similarity to Sphingomonas azotifigens. The population of diazotrophic bacteria showed great numbers present in the rice roots. There was an increase in grain yield of rice by 48% when inoculated with some strains of Sphingomonas spp. and Herbaspirillum seropedicae (ZAE94) on the treatment without inoculation and without nitrogen. |
publishDate |
2008 |
dc.date.issued.fl_str_mv |
2008-02-27 |
dc.date.available.fl_str_mv |
2009-09-14 |
dc.date.accessioned.fl_str_mv |
2016-04-26T19:36:55Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
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publishedVersion |
dc.identifier.citation.fl_str_mv |
VIDEIRA, Sandy Sampaio. Taxonomia polif?sica de bact?rias diazotr?ficas do g?nero Sphingomonas spp. e efeito da inocula??o em plantas de arroz. 2008. 140 f. Disserta??o (Mestrado em Agronomia, Ci?ncia do Solo) - Instituto de Agronomia,Universidade Federal Rural do Rio de Janeiro, Serop?dica - RJ, 2008. |
dc.identifier.uri.fl_str_mv |
https://tede.ufrrj.br/jspui/handle/tede/270 |
identifier_str_mv |
VIDEIRA, Sandy Sampaio. Taxonomia polif?sica de bact?rias diazotr?ficas do g?nero Sphingomonas spp. e efeito da inocula??o em plantas de arroz. 2008. 140 f. Disserta??o (Mestrado em Agronomia, Ci?ncia do Solo) - Instituto de Agronomia,Universidade Federal Rural do Rio de Janeiro, Serop?dica - RJ, 2008. |
url |
https://tede.ufrrj.br/jspui/handle/tede/270 |
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por |
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Universidade Federal Rural do Rio de Janeiro |
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Programa de P?s-Gradua??o em Agronomia - Ci?ncia do Solo |
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UFRRJ |
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Brasil |
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Instituto de Agronomia |
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Universidade Federal Rural do Rio de Janeiro |
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