Sele??o de bact?rias diazotr?ficas solubilizadoras de f?sforo e seu efeito no desenvolvimento de plantas de arroz

Detalhes bibliográficos
Ano de defesa: 2011
Autor(a) principal: Estrada Bonilla, German Andres lattes
Orientador(a): Baldani, Jos? Ivo lattes
Banca de defesa: Não Informado pela instituição
Tipo de documento: Dissertação
Tipo de acesso: Acesso aberto
Idioma: por
Instituição de defesa: Universidade Federal Rural do Rio de Janeiro
Programa de Pós-Graduação: Programa de P?s-Gradua??o em Fitotecnia
Departamento: Instituto de Agronomia
País: Brasil
Palavras-chave em Português:
Compostos ind?licos, Fixa??o biol?gica de nitrog?nio, Bact?rias promotoras do crescimento vegetal
Palavras-chave em Inglês:
Indolic compounds, Biological nitrogen fixation, Plant growth promoting bacteria.
Área do conhecimento CNPq:
Agronomia
Link de acesso: https://tede.ufrrj.br/jspui/handle/jspui/1166
Resumo: Rice (Oryza sativa L.) is one of the major world crops, being widely consumed among people from five continents. In recent years research have increased in relation to the use of plant growth promoting diazotrophic bacteria (PGPB) as a possible solution to reduce the use of chemical nitrogen and phosphate fertilizers. Thus, this study aimed to evaluate the physiological potential and inorganic phosphate (IP) solubilization capacity of different diazotrophic strains isolated from rice plants grown in different regions of Brazil as well as to evaluate their role in the development of rice plants. Firstly, the culture media employed to test the phosphate solubilization in petri dishes were standardized. In this study 49 strains were tested for their capability of solibilizing tricalcium phosphate in NBRIP and GL solid media. Afterwards, the soluble P, pH, population and the index of solubilization (IS) were quantified for 7 IP solubilizing strains grown in the NBRIP liquid medium. The IP solubilizing isolates were also tested for the production of indolic compounds (IAA) and the nitrogen fixation capacity through the acetylene reduction activity (ARA). The isolates were taxonomical identified through the amplification and partial sequencing of 16S RNAr and nifH genes. Three of these strains and the controls H. seropedicae ZAE94, G diazotrophicus PAL5 and A.chroochoccum AC1 were tested in greenhouse conditions in association with rice plants. All strains that solubilized IP in NBRIP liquid medium decreased the medium pH while the bacterial population remained around 108 cfu ml-1 for up to 15 days. All strains were capable to produce indole compounds and reduced acetylene. The partial analysis of 16S RNAr and nifH gene indicated the identification of the species Herbaspirillum seropedicae, Burkholderia vietnamiensis and B. kururiensis species. All strains showed an increase in the range of 33 and 47% in grain yield when tricalcium phosphate (PTC) was applied and between 18 and 44% when PSS (simple superphosphate) was used. In general, all of the strains stimulated an increase of total P in the grain and a greater harvest index. The use of these strains as bioinculant is promising and therefore it is necessary to test in the field conditions
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spelling Baldani, Jos? Ivo53886445887http://lattes.cnpq.br/8391182235603982Baldani, Vera Lucia Divan44324928720http://lattes.cnpq.br/74459966397986246096062717http://lattes.cnpq.br/4285048067075293Estrada Bonilla, German Andres2016-08-17T14:23:26Z2011-06-09ESTRADA BONILLA, German Andres. Sele??o de bact?rias diazotr?ficas solubilizadoras de f?sforo e seu efeito no desenvolvimento de plantas de arroz. 2011. 70 f. Disserta??o (Mestrado em Fitotecnia) - Instituto de Agronomia, Universidade Federal Rural do Rio de Janeiro, Serop?dica, 2011.https://tede.ufrrj.br/jspui/handle/jspui/1166Rice (Oryza sativa L.) is one of the major world crops, being widely consumed among people from five continents. In recent years research have increased in relation to the use of plant growth promoting diazotrophic bacteria (PGPB) as a possible solution to reduce the use of chemical nitrogen and phosphate fertilizers. Thus, this study aimed to evaluate the physiological potential and inorganic phosphate (IP) solubilization capacity of different diazotrophic strains isolated from rice plants grown in different regions of Brazil as well as to evaluate their role in the development of rice plants. Firstly, the culture media employed to test the phosphate solubilization in petri dishes were standardized. In this study 49 strains were tested for their capability of solibilizing tricalcium phosphate in NBRIP and GL solid media. Afterwards, the soluble P, pH, population and the index of solubilization (IS) were quantified for 7 IP solubilizing strains grown in the NBRIP liquid medium. The IP solubilizing isolates were also tested for the production of indolic compounds (IAA) and the nitrogen fixation capacity through the acetylene reduction activity (ARA). The isolates were taxonomical identified through the amplification and partial sequencing of 16S RNAr and nifH genes. Three of these strains and the controls H. seropedicae ZAE94, G diazotrophicus PAL5 and A.chroochoccum AC1 were tested in greenhouse conditions in association with rice plants. All strains that solubilized IP in NBRIP liquid medium decreased the medium pH while the bacterial population remained around 108 cfu ml-1 for up to 15 days. All strains were capable to produce indole compounds and reduced acetylene. The partial analysis of 16S RNAr and nifH gene indicated the identification of the species Herbaspirillum seropedicae, Burkholderia vietnamiensis and B. kururiensis species. All strains showed an increase in the range of 33 and 47% in grain yield when tricalcium phosphate (PTC) was applied and between 18 and 44% when PSS (simple superphosphate) was used. In general, all of the strains stimulated an increase of total P in the grain and a greater harvest index. The use of these strains as bioinculant is promising and therefore it is necessary to test in the field conditionsO arroz (Oryza sativa L.) ? uma das principais culturas cultivadas, sendo amplamente consumida nos cinco continentes. Nos ?ltimos anos as pesquisas t?m aumentado em rela??o ao uso de bact?rias diazotr?ficas e promotoras de crescimento vegetal (BPCV), como uma poss?vel solu??o para diminuir o uso de fertilizantes qu?micos nitrogenados e fosfatados. Desta forma, este trabalho teve por objetivo avaliar o potencial fisiol?gico e a capacidade de solubiliza??o de fosfato inorg?nico (PI) de diferentes estirpes de bact?rias diazotr?ficas oriundas de plantas de arroz cultivadas em diferentes regi?es do Brasil e seu papel no desenvolvimento de plantas de arroz. Primeiramente foi padronizado o meio de cultura a ser empregado nos ensaios de solubiliza??o de fosfato inorg?nico em placas de petri. Neste estudo foram testadas 49 estirpes em quanto sua capacidade de solubilizar fosfato tric?lcico (PTC) nos meios s?lidos NBRIP e GL. Em seguida foi quantificado o P sol?vel, pH, a popula??o de bact?rias e ?ndice de solubiliza??o (IS) das 7 estirpes solubilizadoras de PI em meio l?quido NBRIP. As estirpes solubilizadoras de PI tamb?m foram testadas quanto ? produ??o de compostos ind?licos (AIA) e capacidade de fixar nitrog?nio atrav?s da atividade de redu??o e acetileno (ARA). As estirpes foram identificadas atrav?s da amplifica??o e do sequenciamento parcial dos genes 16S RNAr e gene nifH. Tr?s destas estirpes foram testadas em condi??es de casa de vegeta??o junto com os controles Herbaspirillum seropedicae BR11417 (ZAE94), Gluconacetobacter diazotophicus BR11281 (PAL5) e Azotobacter Chroochoccum AC1 quanto ? capacidade de aumentar o desenvolvimento e produ??o de plantas de arroz. Todas as estirpes que solubilizaram PI no meio de cultura NBRIP l?quido influenciaram na diminui??o do pH do meio enquanto que a popula??o se manteve em torno de 108 ufc.ml-1 por at? 15 dias. Todas as estirpes apresentaram capacidade de produzir compostos ind?licos e foram capazes de reduzir acetileno. A an?lise parcial do gene 16S RNAr e do gene nifH das sete estirpes possibilitou a identifica??o das esp?cies Herbaspirillum seropedicae, Burkholderia vietnamiensis e B. kururiensis. Todas as estirpes promoveram um aumento na produ??o de massa seca nos gr?os entre 33 e 47% quando foi utilizado o PTC e de 18 a 44% quando foi utilizado o fosfato super simples (PSS). Em geral, todas as estirpes estimularam um maior acumulo de N e P total no gr?o e um maior ?ndice de colheita. O uso dessas estirpes como bioinoculante ? bastante promissor e portanto torna-se necess?rio test?-las em condi??es de campo.Submitted by Sandra Pereira (srpereira@ufrrj.br) on 2016-08-17T14:23:26Z No. of bitstreams: 1 2011- German Andres Estrada Bonilla.pdf: 1652819 bytes, checksum: 24a4d4664a65dfad88ccdfe17b4a0c02 (MD5)Made available in DSpace on 2016-08-17T14:23:26Z (GMT). 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dc.title.por.fl_str_mv Sele??o de bact?rias diazotr?ficas solubilizadoras de f?sforo e seu efeito no desenvolvimento de plantas de arroz
title Sele??o de bact?rias diazotr?ficas solubilizadoras de f?sforo e seu efeito no desenvolvimento de plantas de arroz
spellingShingle Sele??o de bact?rias diazotr?ficas solubilizadoras de f?sforo e seu efeito no desenvolvimento de plantas de arroz
Estrada Bonilla, German Andres
Indolic compounds, Biological nitrogen fixation, Plant growth promoting bacteria.
Compostos ind?licos, Fixa??o biol?gica de nitrog?nio, Bact?rias promotoras do crescimento vegetal
Agronomia
title_short Sele??o de bact?rias diazotr?ficas solubilizadoras de f?sforo e seu efeito no desenvolvimento de plantas de arroz
title_full Sele??o de bact?rias diazotr?ficas solubilizadoras de f?sforo e seu efeito no desenvolvimento de plantas de arroz
title_fullStr Sele??o de bact?rias diazotr?ficas solubilizadoras de f?sforo e seu efeito no desenvolvimento de plantas de arroz
title_full_unstemmed Sele??o de bact?rias diazotr?ficas solubilizadoras de f?sforo e seu efeito no desenvolvimento de plantas de arroz
title_sort Sele??o de bact?rias diazotr?ficas solubilizadoras de f?sforo e seu efeito no desenvolvimento de plantas de arroz
author Estrada Bonilla, German Andres
author_facet Estrada Bonilla, German Andres
author_role author
dc.contributor.advisor1.fl_str_mv Baldani, Jos? Ivo
dc.contributor.advisor1ID.fl_str_mv 53886445887
dc.contributor.advisor1Lattes.fl_str_mv http://lattes.cnpq.br/8391182235603982
dc.contributor.advisor-co1.fl_str_mv Baldani, Vera Lucia Divan
dc.contributor.advisor-co1ID.fl_str_mv 44324928720
dc.contributor.advisor-co1Lattes.fl_str_mv http://lattes.cnpq.br/7445996639798624
dc.contributor.authorID.fl_str_mv 6096062717
dc.contributor.authorLattes.fl_str_mv http://lattes.cnpq.br/4285048067075293
dc.contributor.author.fl_str_mv Estrada Bonilla, German Andres
contributor_str_mv Baldani, Jos? Ivo
Baldani, Vera Lucia Divan
dc.subject.eng.fl_str_mv Indolic compounds, Biological nitrogen fixation, Plant growth promoting bacteria.
topic Indolic compounds, Biological nitrogen fixation, Plant growth promoting bacteria.
Compostos ind?licos, Fixa??o biol?gica de nitrog?nio, Bact?rias promotoras do crescimento vegetal
Agronomia
dc.subject.por.fl_str_mv Compostos ind?licos, Fixa??o biol?gica de nitrog?nio, Bact?rias promotoras do crescimento vegetal
dc.subject.cnpq.fl_str_mv Agronomia
description Rice (Oryza sativa L.) is one of the major world crops, being widely consumed among people from five continents. In recent years research have increased in relation to the use of plant growth promoting diazotrophic bacteria (PGPB) as a possible solution to reduce the use of chemical nitrogen and phosphate fertilizers. Thus, this study aimed to evaluate the physiological potential and inorganic phosphate (IP) solubilization capacity of different diazotrophic strains isolated from rice plants grown in different regions of Brazil as well as to evaluate their role in the development of rice plants. Firstly, the culture media employed to test the phosphate solubilization in petri dishes were standardized. In this study 49 strains were tested for their capability of solibilizing tricalcium phosphate in NBRIP and GL solid media. Afterwards, the soluble P, pH, population and the index of solubilization (IS) were quantified for 7 IP solubilizing strains grown in the NBRIP liquid medium. The IP solubilizing isolates were also tested for the production of indolic compounds (IAA) and the nitrogen fixation capacity through the acetylene reduction activity (ARA). The isolates were taxonomical identified through the amplification and partial sequencing of 16S RNAr and nifH genes. Three of these strains and the controls H. seropedicae ZAE94, G diazotrophicus PAL5 and A.chroochoccum AC1 were tested in greenhouse conditions in association with rice plants. All strains that solubilized IP in NBRIP liquid medium decreased the medium pH while the bacterial population remained around 108 cfu ml-1 for up to 15 days. All strains were capable to produce indole compounds and reduced acetylene. The partial analysis of 16S RNAr and nifH gene indicated the identification of the species Herbaspirillum seropedicae, Burkholderia vietnamiensis and B. kururiensis species. All strains showed an increase in the range of 33 and 47% in grain yield when tricalcium phosphate (PTC) was applied and between 18 and 44% when PSS (simple superphosphate) was used. In general, all of the strains stimulated an increase of total P in the grain and a greater harvest index. The use of these strains as bioinculant is promising and therefore it is necessary to test in the field conditions
publishDate 2011
dc.date.issued.fl_str_mv 2011-06-09
dc.date.accessioned.fl_str_mv 2016-08-17T14:23:26Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
format masterThesis
status_str publishedVersion
dc.identifier.citation.fl_str_mv ESTRADA BONILLA, German Andres. Sele??o de bact?rias diazotr?ficas solubilizadoras de f?sforo e seu efeito no desenvolvimento de plantas de arroz. 2011. 70 f. Disserta??o (Mestrado em Fitotecnia) - Instituto de Agronomia, Universidade Federal Rural do Rio de Janeiro, Serop?dica, 2011.
dc.identifier.uri.fl_str_mv https://tede.ufrrj.br/jspui/handle/jspui/1166
identifier_str_mv ESTRADA BONILLA, German Andres. Sele??o de bact?rias diazotr?ficas solubilizadoras de f?sforo e seu efeito no desenvolvimento de plantas de arroz. 2011. 70 f. Disserta??o (Mestrado em Fitotecnia) - Instituto de Agronomia, Universidade Federal Rural do Rio de Janeiro, Serop?dica, 2011.
url https://tede.ufrrj.br/jspui/handle/jspui/1166
dc.language.iso.fl_str_mv por
language por
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