Ceratocystis fimbriata Ellis & Halsted em espécies florestais no Rio Grande do Sul: comportamento e controle biológico
Ano de defesa: | 2014 |
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Autor(a) principal: | |
Orientador(a): | |
Banca de defesa: | , |
Tipo de documento: | Dissertação |
Tipo de acesso: | Acesso aberto |
Idioma: | por |
Instituição de defesa: |
Universidade Federal de Santa Maria
|
Programa de Pós-Graduação: |
Programa de Pós-Graduação em Engenharia Florestal
|
Departamento: |
Recursos Florestais e Engenharia Florestal
|
País: |
BR
|
Palavras-chave em Português: | |
Palavras-chave em Inglês: | |
Área do conhecimento CNPq: | |
Link de acesso: | http://repositorio.ufsm.br/handle/1/8734 |
Resumo: | Pathogens of the genus Ceratocystis are polyphagous, attacking several species of economic importance, such as black wattle, eucalyptus, mango and cocoa, these have wide variability even within a single genus or species, the result of adaptations to the environment they live in and the hosts available. Study the behavior of these pathogens such as Ceratocystis fimbriata in forest species is important to understand the ways of survival variability and fungus resistance mainly native species. The use of biocontrol as of the genera Trichoderma and Bacillus, have been widely studied as an alternative for the biological control of soil borne pathogens. Hence, the present work aims to study the behavior of different isolates of C. fimbriata obtained from kiwi (Actinidia deliciosa) in forest species as black wattle (Acacia mearnsii), guava (Psidium cattleianum), araucaria (Araucaria angustifolia), cherry (Eugenia involucrata) and inga (Inga marginata); potential biocontrol in vitro Trichoderma spp. and Bacillus subtilis about C. fimbriata and the in vivo behavior of B. subtilis in seedlings of black wattle , against C. fimbriata as an alternative control . For this purpose, we used isolates of C. fimbriata obtained and identified in kiwi plantations. In the pathogenicity test isolates were evaluated for their pathogenic variability and behavior on seedlings of black wattle, guava, araucaria, cherry and inga. Through direct confrontation testing was analyzed in vitro antagonistic potential of Trichoderma spp. and B. subtilis on the pathogen. For the evaluation of the in vivo behavior of B. subtilis black wattle seedlings were inoculated with product Rizolyptus®, seven days before and seven days after pathogen inoculation. Seedlings inoculated with native species C. fimbriata showed no sign of wilting or discoloration of the tissues. However, in seedlings of Acacia mearnsii isolates of C. fimbriata exhibited severity percentage ranging from 44.15 to 100%. In direct confrontation testing isolates of Trichoderma spp. and B. subtilis demonstrated to be effective in vitro on biocontrol isolates of C. fimbriata with percentages of inhibition ranging from 46.48 to 57.76 and from 14.12 to 32.20 % respectively. In biocontrol in vivo test, the Rizolyptus® product was not effective in controlling C. fimbriata and all black wattle seedlings inoculated with the pathogen showed symptoms of wilting and death. |
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2014-11-102014-11-102014-02-25MEZZOMO, Ricardo. Ceratocystis fimbriata Ellis & Halsted in forest species in Rio Grande do Sul state: behavior and biological control. 2014. 78 f. Dissertação (Mestrado em Recursos Florestais e Engenharia Florestal) - Universidade Federal de Santa Maria, Santa Maria, 2014.http://repositorio.ufsm.br/handle/1/8734Pathogens of the genus Ceratocystis are polyphagous, attacking several species of economic importance, such as black wattle, eucalyptus, mango and cocoa, these have wide variability even within a single genus or species, the result of adaptations to the environment they live in and the hosts available. Study the behavior of these pathogens such as Ceratocystis fimbriata in forest species is important to understand the ways of survival variability and fungus resistance mainly native species. The use of biocontrol as of the genera Trichoderma and Bacillus, have been widely studied as an alternative for the biological control of soil borne pathogens. Hence, the present work aims to study the behavior of different isolates of C. fimbriata obtained from kiwi (Actinidia deliciosa) in forest species as black wattle (Acacia mearnsii), guava (Psidium cattleianum), araucaria (Araucaria angustifolia), cherry (Eugenia involucrata) and inga (Inga marginata); potential biocontrol in vitro Trichoderma spp. and Bacillus subtilis about C. fimbriata and the in vivo behavior of B. subtilis in seedlings of black wattle , against C. fimbriata as an alternative control . For this purpose, we used isolates of C. fimbriata obtained and identified in kiwi plantations. In the pathogenicity test isolates were evaluated for their pathogenic variability and behavior on seedlings of black wattle, guava, araucaria, cherry and inga. Through direct confrontation testing was analyzed in vitro antagonistic potential of Trichoderma spp. and B. subtilis on the pathogen. For the evaluation of the in vivo behavior of B. subtilis black wattle seedlings were inoculated with product Rizolyptus®, seven days before and seven days after pathogen inoculation. Seedlings inoculated with native species C. fimbriata showed no sign of wilting or discoloration of the tissues. However, in seedlings of Acacia mearnsii isolates of C. fimbriata exhibited severity percentage ranging from 44.15 to 100%. In direct confrontation testing isolates of Trichoderma spp. and B. subtilis demonstrated to be effective in vitro on biocontrol isolates of C. fimbriata with percentages of inhibition ranging from 46.48 to 57.76 and from 14.12 to 32.20 % respectively. In biocontrol in vivo test, the Rizolyptus® product was not effective in controlling C. fimbriata and all black wattle seedlings inoculated with the pathogen showed symptoms of wilting and death.Patógenos do gênero Ceratocystis são polífagos, atacando diversas espécies com importância econômica, como a acácia-negra, eucalipto, mangueira e cacau, estes possuem ampla variabilidade, mesmo dentro de um único gênero ou espécie, resultado de adaptações ao meio em que vivem e aos hospedeiros disponíveis. Estudar o comportamento destes patógenos, como Ceratocystis fimbriata, em espécies florestais é importante para compreender as formas de sobrevivência, variabilidade e resistência do fungo principalmente em espécies nativas. O uso de biocontroladores como, dos gêneros Trichoderma e Bacillus, tem sido largamente estudado como alternativa para o controle biológico de patógenos de solo. Diante disto, o presente trabalho tem como objetivo estudar o comportamento de diferentes isolados de C. fimbriata obtidos de kiwi (Actinidia deliciosa) em espécies florestais como, acácia-negra (Acacia mearnsii), araçá (Psidium cattleianum), araucária (Araucaria angustifolia), cerejeira (Eugenia involucrata) e ingá (Inga marginata); o potencial de biocontrole in vitro de Trichoderma spp. e Bacillus subtilis sobre C. fimbriata e o comportamento in vivo de B. subtilis em mudas de acácia-negra, frente a C. fimbriata, como alternativa de controle. Para tanto, foram utilizados isolados de C. fimbriata obtidos e identificados em plantios de kiwi. No teste de patogenicidade cruzada os isolados foram avaliados quanto à sua variabilidade patogênica e o comportamento sobre mudas de acácia-negra, araçá, araucária, cerejeira e ingá. Através de testes de confrontação direta foi analisado o potencial antagônico in vitro de Trichoderma spp. e B. subtilis sobre o patógeno. Para a avaliação do comportamento in vivo de B. subtilis as mudas de acácia-negra foram inoculadas com o produto Rizolyptus®, sete dias antes e após a inoculação do patógeno. As mudas de espécies nativas inoculadas com C. fimbriata não exibiram sintoma de murcha ou descoloração dos tecidos. Entretanto, em mudas de acácia-negra os isolados de C. fimbriata exibiram percentuais de severidade que variam entre 44,15 a 100%. Nos testes de confrontação direta os isolados de Trichoderma spp. e B. subtilis demostraram-se eficientes no biocontrole in vitro sobre os isolados de C. fimbriata com percentuais de inibição variando de 46,48 a 57,76 e 14,12 a 32,20% respectivamente. No teste de biocontrole in vivo, o produto Rizolyptus® não foi eficiente no controle de C. fimbriata e todas as mudas de acácia-negra inoculadas com o patógeno apresentaram sintomas de murcha e morte.Coordenação de Aperfeiçoamento de Pessoal de Nível Superiorapplication/pdfporUniversidade Federal de Santa MariaPrograma de Pós-Graduação em Engenharia FlorestalUFSMBRRecursos Florestais e Engenharia FlorestalMurcha-de-ceratocystisPatogenicidadeEspécies nativasCeratocystis wiltBiological controlNative speciesCNPQ::CIENCIAS AGRARIAS::RECURSOS FLORESTAIS E ENGENHARIA FLORESTALCeratocystis fimbriata Ellis & Halsted em espécies florestais no Rio Grande do Sul: comportamento e controle biológicoCeratocystis fimbriata Ellis & Halsted in forest species in Rio Grande do Sul state: behavior and biological controlinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisMuniz, Marlove Fatima Briãohttp://lattes.cnpq.br/3148312031889388Kulczynski, Stela Marishttp://lattes.cnpq.br/0604511258822899Poletto, Igorhttp://lattes.cnpq.br/5851156257793439http://lattes.cnpq.br/9324287744602947Mezzomo, Ricardo500200000003400500300500500e8de5014-a63f-4739-8254-6b23c08bcd59ea9f69c6-ce05-44bb-a3c6-ad627f974e05846f6a13-7123-4ed9-b484-6800cf12b2f35dfc8864-aaf7-44f5-b487-910c74a8a17ainfo:eu-repo/semantics/openAccessreponame:Manancial - Repositório Digital da UFSMinstname:Universidade Federal de Santa Maria (UFSM)instacron:UFSMORIGINALMEZZOMO, RICARDO.pdfapplication/pdf1119184http://repositorio.ufsm.br/bitstream/1/8734/1/MEZZOMO%2c%20RICARDO.pdf2900ccd419e0591c0056f8e519aeb9a0MD51TEXTMEZZOMO, RICARDO.pdf.txtMEZZOMO, RICARDO.pdf.txtExtracted texttext/plain139772http://repositorio.ufsm.br/bitstream/1/8734/2/MEZZOMO%2c%20RICARDO.pdf.txt1dea99a22683b03babca73666e60bb9bMD52THUMBNAILMEZZOMO, RICARDO.pdf.jpgMEZZOMO, RICARDO.pdf.jpgIM Thumbnailimage/jpeg4974http://repositorio.ufsm.br/bitstream/1/8734/3/MEZZOMO%2c%20RICARDO.pdf.jpg085ee11e434fa8c86abb616039d31e33MD531/87342022-07-12 10:55:00.808oai:repositorio.ufsm.br:1/8734Repositório Institucionalhttp://repositorio.ufsm.br/PUBhttp://repositorio.ufsm.br/oai/requestopendoar:39132022-07-12T13:55Manancial - Repositório Digital da UFSM - Universidade Federal de Santa Maria (UFSM)false |
dc.title.por.fl_str_mv |
Ceratocystis fimbriata Ellis & Halsted em espécies florestais no Rio Grande do Sul: comportamento e controle biológico |
dc.title.alternative.eng.fl_str_mv |
Ceratocystis fimbriata Ellis & Halsted in forest species in Rio Grande do Sul state: behavior and biological control |
title |
Ceratocystis fimbriata Ellis & Halsted em espécies florestais no Rio Grande do Sul: comportamento e controle biológico |
spellingShingle |
Ceratocystis fimbriata Ellis & Halsted em espécies florestais no Rio Grande do Sul: comportamento e controle biológico Mezzomo, Ricardo Murcha-de-ceratocystis Patogenicidade Espécies nativas Ceratocystis wilt Biological control Native species CNPQ::CIENCIAS AGRARIAS::RECURSOS FLORESTAIS E ENGENHARIA FLORESTAL |
title_short |
Ceratocystis fimbriata Ellis & Halsted em espécies florestais no Rio Grande do Sul: comportamento e controle biológico |
title_full |
Ceratocystis fimbriata Ellis & Halsted em espécies florestais no Rio Grande do Sul: comportamento e controle biológico |
title_fullStr |
Ceratocystis fimbriata Ellis & Halsted em espécies florestais no Rio Grande do Sul: comportamento e controle biológico |
title_full_unstemmed |
Ceratocystis fimbriata Ellis & Halsted em espécies florestais no Rio Grande do Sul: comportamento e controle biológico |
title_sort |
Ceratocystis fimbriata Ellis & Halsted em espécies florestais no Rio Grande do Sul: comportamento e controle biológico |
author |
Mezzomo, Ricardo |
author_facet |
Mezzomo, Ricardo |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Muniz, Marlove Fatima Brião |
dc.contributor.advisor1Lattes.fl_str_mv |
http://lattes.cnpq.br/3148312031889388 |
dc.contributor.referee1.fl_str_mv |
Kulczynski, Stela Maris |
dc.contributor.referee1Lattes.fl_str_mv |
http://lattes.cnpq.br/0604511258822899 |
dc.contributor.referee2.fl_str_mv |
Poletto, Igor |
dc.contributor.referee2Lattes.fl_str_mv |
http://lattes.cnpq.br/5851156257793439 |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/9324287744602947 |
dc.contributor.author.fl_str_mv |
Mezzomo, Ricardo |
contributor_str_mv |
Muniz, Marlove Fatima Brião Kulczynski, Stela Maris Poletto, Igor |
dc.subject.por.fl_str_mv |
Murcha-de-ceratocystis Patogenicidade Espécies nativas |
topic |
Murcha-de-ceratocystis Patogenicidade Espécies nativas Ceratocystis wilt Biological control Native species CNPQ::CIENCIAS AGRARIAS::RECURSOS FLORESTAIS E ENGENHARIA FLORESTAL |
dc.subject.eng.fl_str_mv |
Ceratocystis wilt Biological control Native species |
dc.subject.cnpq.fl_str_mv |
CNPQ::CIENCIAS AGRARIAS::RECURSOS FLORESTAIS E ENGENHARIA FLORESTAL |
description |
Pathogens of the genus Ceratocystis are polyphagous, attacking several species of economic importance, such as black wattle, eucalyptus, mango and cocoa, these have wide variability even within a single genus or species, the result of adaptations to the environment they live in and the hosts available. Study the behavior of these pathogens such as Ceratocystis fimbriata in forest species is important to understand the ways of survival variability and fungus resistance mainly native species. The use of biocontrol as of the genera Trichoderma and Bacillus, have been widely studied as an alternative for the biological control of soil borne pathogens. Hence, the present work aims to study the behavior of different isolates of C. fimbriata obtained from kiwi (Actinidia deliciosa) in forest species as black wattle (Acacia mearnsii), guava (Psidium cattleianum), araucaria (Araucaria angustifolia), cherry (Eugenia involucrata) and inga (Inga marginata); potential biocontrol in vitro Trichoderma spp. and Bacillus subtilis about C. fimbriata and the in vivo behavior of B. subtilis in seedlings of black wattle , against C. fimbriata as an alternative control . For this purpose, we used isolates of C. fimbriata obtained and identified in kiwi plantations. In the pathogenicity test isolates were evaluated for their pathogenic variability and behavior on seedlings of black wattle, guava, araucaria, cherry and inga. Through direct confrontation testing was analyzed in vitro antagonistic potential of Trichoderma spp. and B. subtilis on the pathogen. For the evaluation of the in vivo behavior of B. subtilis black wattle seedlings were inoculated with product Rizolyptus®, seven days before and seven days after pathogen inoculation. Seedlings inoculated with native species C. fimbriata showed no sign of wilting or discoloration of the tissues. However, in seedlings of Acacia mearnsii isolates of C. fimbriata exhibited severity percentage ranging from 44.15 to 100%. In direct confrontation testing isolates of Trichoderma spp. and B. subtilis demonstrated to be effective in vitro on biocontrol isolates of C. fimbriata with percentages of inhibition ranging from 46.48 to 57.76 and from 14.12 to 32.20 % respectively. In biocontrol in vivo test, the Rizolyptus® product was not effective in controlling C. fimbriata and all black wattle seedlings inoculated with the pathogen showed symptoms of wilting and death. |
publishDate |
2014 |
dc.date.accessioned.fl_str_mv |
2014-11-10 |
dc.date.available.fl_str_mv |
2014-11-10 |
dc.date.issued.fl_str_mv |
2014-02-25 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
MEZZOMO, Ricardo. Ceratocystis fimbriata Ellis & Halsted in forest species in Rio Grande do Sul state: behavior and biological control. 2014. 78 f. Dissertação (Mestrado em Recursos Florestais e Engenharia Florestal) - Universidade Federal de Santa Maria, Santa Maria, 2014. |
dc.identifier.uri.fl_str_mv |
http://repositorio.ufsm.br/handle/1/8734 |
identifier_str_mv |
MEZZOMO, Ricardo. Ceratocystis fimbriata Ellis & Halsted in forest species in Rio Grande do Sul state: behavior and biological control. 2014. 78 f. Dissertação (Mestrado em Recursos Florestais e Engenharia Florestal) - Universidade Federal de Santa Maria, Santa Maria, 2014. |
url |
http://repositorio.ufsm.br/handle/1/8734 |
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openAccess |
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Universidade Federal de Santa Maria |
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Programa de Pós-Graduação em Engenharia Florestal |
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UFSM |
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BR |
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Recursos Florestais e Engenharia Florestal |
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Universidade Federal de Santa Maria |
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