Desenvolvimento de diagramas bifásicos de sistemas constituídos por copolímeros-sais e líquido iônico-sais para aplicação na partição de proteínas

• Ano de defesa: 2013
• Autor(a) principal: Zeferino, Lilian Bozzi
• Orientador(a): Minim, Luis Antonio
• Banca de defesa: Eller, Monique Renon , Pires, Ana Clarissa dos Santos , Peña, Wilmer Edgard Luera
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Federal de Viçosa
• Programa de Pós-Graduação: Mestrado em Ciência e Tecnologia de Alimentos
• Departamento: Ciência de Alimentos; Tecnologia de Alimentos; Engenharia de Alimentos
• País: BR
• Palavras-chave em Português: Sistema aquoso bifásico; Líquidos iônicos; Diagrama de equilíbrio; Partição; Proteína
• Palavras-chave em Inglês: Biphasic aqueous system; Ionic liquids; Balance diagram; Partition; Protein
• Área do conhecimento CNPq: CNPQ::CIENCIAS AGRARIAS::CIENCIA E TECNOLOGIA DE ALIMENTOS::TECNOLOGIA DE ALIMENTOS
• Link de acesso: http://locus.ufv.br/handle/123456789/2934

Aqueous two phase systems (ATPS) have been used with great success in processes of separation, concentration and purification of bioactive molecules, since they are a variant of conventional liquid-liquid extraction, which favor the stability of biomolecules. As the water component present in the largest amount in both phases, these systems provide an environment for the biomolecules mild preserving thus its stability and avoiding loss of molecular biological activities. Therefore, this study was obtained new equilibrium data for systems formed by ionic liquid ([C4min] [BF4]) + Li2SO4 + water, ionic liquid ([C4min] [BF4]) + MnSO4 + water; copolymer (F68) + C4H4NaO6 + water, and copolymer (F68) + C4H4K2O6 + water at 25 ° C. For systems consisting of ionic liquid and sulfate it was observed that by changing the cation of the sulphate added to the system, there was a tendency of increased region to the biphasic curve Li2SO4 higher concentrations of salt. In the case of copolymer systems formed of organic and salt were also significant shift of the two phase region. For the system in which it was used C4H4NaO6 the region with the formation of two phases is greater. Subsequently, was used these new data in the study of partition of the whey proteins α-lactalbumin (α-la) and β-lactoglobulin (β-lg). In this step sought to partition the proteins so obtain two fractions, one rich in β-lg and the other rich in α-la. Were studied the effects of the types of salts used in training systems on the partition coefficient of the protein of interest. The systems formed by [C4min] [BF4], Li2SO4 and water, able to partition the proteins, where the highest concentration of α-la was detected in the lower phase (rich in ionic liquid). Already β-lg partitioned to saline phase (upper phase). For systems consisting of [C4min] [BF4], MnSO4 and water, exclusively for both proteins separated to lower phase. However, during the experiments it was observed protein precipitation at the interface systems. Systems already formed by copolymer (F68) C4H4NaO6 and water, and copolymer (F68) C4H4K2O6 and water separated proteins of interest only for the saline phase (lower phase), in other words, the systems were not able to separate the proteins of interest in two distinct phases.