Histomorfometria e expressão imunoistoquímica de RANKL em fêmur e vértebra de ratos com osteoporose secundária

• Ano de defesa: 2007
• Autor(a) principal: Valente, Fabrício Luciani
• Orientador(a): Viloria, Marlene Isabel Vargas
• Banca de defesa: Serakides, Rogéria , Borges, Andrea Pacheco Batista
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Federal de Viçosa
• Programa de Pós-Graduação: Mestrado em Medicina Veterinária
• Departamento: Biotecnologia, diagnóstico e controle de doenças; Epidemiologia e controle de qualidade de prod. de
• País: BR
• Palavras-chave em Português: Osteoporose; RANKL; Rato
• Palavras-chave em Inglês: Osteoporosis; RANKL; Rats
• Área do conhecimento CNPq: CNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIA::PATOLOGIA ANIMAL::PATOLOGIA CLINICA ANIMAL
• Link de acesso: http://locus.ufv.br/handle/123456789/4972

Osteoporosis is a common human disease affecting both men and women, and it can classified as (1) primary, related to sexual hormones deficiency or senility, or (2) secondary, for what the most common example is the chronic therapies with glucocorticoids. Whatever is its cause, the osteoporosis outcomes are bone loss and increased fracture risk. Although osteoporosis is considered a systemic condition, bone loss seen in osteoporosis is not homogeneous throughout the skeleton. RANKL is a cytokine able to activate the osteoclasts function and its expression is inducible in osteoblasts and T cells by a range of stimuli. Function of RANKL has been considered a possible therapeutic target for the treatment of osteoporosis. To evaluate the trabecular bone loss related to the RANKL expression, immunochemistry and histomorphometric assays were used in femur and vertebra of castrated and/or glucocorticoid-treated male and female rats, at the day 56 after induction. RANKL expression was evident only in the castrated group, both male and female, but not in the group of castrated rats that also received glucocorticoid therapy. But histomorphometric data showed that bone loss was similar in both groups. That could happen because glucocorticoid can inhibit osteoblast metabolism. Histomorphometry also reveals that trabecular bone mass in male is similar to female, and bone loss is not homogenous between distal and proximal femur and vertebra body. At the day 56 after induction, bone loss, in femur and vertebra, both male and female, was compatible to osteoporosis.