Fungos nematófagos predadores no controle de ancilostomatídeos de cães

• Ano de defesa: 2009
• Autor(a) principal: Maciel, Alessandro Spalenza
• Orientador(a): Araújo, Jackson Victor de
• Banca de defesa: Rodrigues, Maria de Lurdes de Azevedo , Furlong, John
• Tipo de documento: Tese
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Federal de Viçosa
• Programa de Pós-Graduação: Doutorado em Medicina Veterinária
• Departamento: Biotecnologia, diagnóstico e controle de doenças; Epidemiologia e controle de qualidade de prod. de
• País: BR
• Palavras-chave em Português: Controle biológico; Ancylostoma spp; Cães
• Palavras-chave em Inglês: Biological control; Ancylostoma spp; Dogs
• Área do conhecimento CNPq: CNPQ::CIENCIAS BIOLOGICAS::PARASITOLOGIA
• Link de acesso: http://locus.ufv.br/handle/123456789/1432

The nematodes of the genus Ancylostoma are cosmopolitan endoparasites of dogs that cause various diseases in these animals and also are zoonotic geohelminths that can infect humans from soil. The control of the adult stage of these nematodes is based on the use of anti-helminthic; however, the use of biocontrol agents may be an additional measure reducing the population of preparasitic stages in development in the soil. This is justified by the fact that stray dogs parasitized are primarily responsible for environmental infestation with hookworm eggs transmitted in faeces. Among the biocontrol organisms, it is known that nematode-trapping fungi have been effective against nematodes of domestic animals. In this context, the objective of this study was to test various fungi isolated of predacious fungi, selecting the most infective for use in the control of infective larval form (L3) of Ancylostoma spp. in soil under semi-natural conditions. The Duddingtonia flagrans fungus (Isolate CG768) was considered the most infective due to the reduction in the recovery of the target nematode L3 and by the characteristic of producing many spores of resistance that can survive in soil, prolonging the life of the fungus in the absence of prey. The infectivity of this isolate could be observed in scanning electron micrographs that showed the destruction of L3 of Ancylostoma spp. in about 48 hours after capture. To test the antagonism in the environment it was used a practical and economically viable system of mass production of fungal inoculum in grains of white rice and milled maize. The fungus was incorporated into the soil colonized in milled maize, taking as parameter the concentration of 10,000 chlamydospores/g of soil, previously effective in vitro tests. This approach probably favored the establishment of the antagonist with significant reduction of the target nematode population in untreated soil under semi-natural conditions, showing the potential regulator of this biological agent.