Avaliação de dois isolados do fungo nematófago Duddingtonia flagrans no controle de larvas infectantes de Ancylostoma spp. de cães

• Ano de defesa: 2013
• Autor(a) principal: Mello, Ingrid Ney Kramer de
• Orientador(a): Araújo, Jackson Victor de
• Banca de defesa: Ferraz, Silamar
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Federal de Viçosa
• Programa de Pós-Graduação: Mestrado em Medicina Veterinária
• Departamento: Biotecnologia, diagnóstico e controle de doenças; Epidemiologia e controle de qualidade de prod. de
• País: BR
• Palavras-chave em Português: Fungo predador; Ancylostoma ssp.; Cães
• Palavras-chave em Inglês: Predator fungus; Ancylostoma ssp.; Dogs
• Área do conhecimento CNPq: CNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIA
• Link de acesso: http://locus.ufv.br/handle/123456789/5129

The nematodes of the genus Ancylostoma are endoparasites of dogs and also zoonotic geohelminths that can infect humans. The control these nematodes in adult stage is based on the use of anthelmintics. However, the use of biocontrol agents may be an additional action to reduce the population in pre-parasitic stages developing in the environment. This study aimed to evaluate the Duddingtonia flagrans predator fungus for the control of the larval form (L3) of Ancylostoma spp., in beach sand. We evaluated the in vitro infectivity of two isolates of the nematophagous fungus D. flagrans (AC001 and CG768) on infective larvae (L3) of Ancylostoma spp. dogs. Vegetative structures (mycelium), reproductive (conidia) and survival (chlamydospores) were used as fungal inoculum. The interaction was evaluated at the end of 10 days of incubation in Petridishes containing agar-water 2% medium in a temperature of 25 ° C. The antagonism in semi-natural conditions was assessed by use of a mass production of fungal inoculum in grains of milled maize. The fungus was introduced into the sand in colonized milled maize at the concentration of 15,000 chlamydospores / gram of sand. This concentration was the most effective in the preliminary in vitro assay (reduction of 59.2%). The results showed the efficiency of the fungus D. flagrans in the control of infective larvae of Ancylostoma spp. in beach sand. This suggests that isolates of this fungus may be used as part of a control program of Ancylostoma spp. in the environment.