Morfologia das glândulas acessórias masculinas e dos espermatozoides em Mischocyttarus (Hymenoptera, Vespidae)

• Ano de defesa: 2011
• Autor(a) principal: Silva, Edalton dos Reis
• Orientador(a): Lino Neto, José
• Banca de defesa: Araújo, Vinícius Albano , Souza, Edmilson Amaral de , Zama, Uyrá dos Santos , Lisboa, Luciane Cristina Oliveira
• Tipo de documento: Tese
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Federal de Viçosa
• Programa de Pós-Graduação: Doutorado em Biologia Celular e Estrutural
• Departamento: Análises quantitativas e moleculares do Genoma; Biologia das células e dos tecidos
• País: BR
• Palavras-chave em Português: Ultraestrutura; Sistema reprodutor; Epitélio
• Palavras-chave em Inglês: Ultrastructure; Reproductive system; Epithelium
• Área do conhecimento CNPq: CNPQ::CIENCIAS BIOLOGICAS::BIOLOGIA GERAL
• Link de acesso: http://locus.ufv.br/handle/123456789/246

Accessory glands and sperm of species Mischocyttarus were subjected to techniques of light microscopy, conventional transmission electron microscopy and ultrastructural cytochemistry. The glandular epithelium of M. rotundicollis, M. injucundus, M. punctatus and M. cerberus is simple, consisting of low cells with rounded to elongated nuclei with decondensed chromatin and evident nucleoli. The cytoplasm of M. rotundicollis and M. injucundus is rich in rough endoplasmic reticulum with many dilated cisterns and free ribosomes. In M. injucundus, free ribosomes and mitochondria were also seen. Granules with shapes, different sizes and different degrees of electron-are seen in M. injucundus, M. rotundicolis and M. cerberus and secretory vesicles with material inside electrondense M. injucundus. In M. punctatus few secretory vesicles were seen in glandular epithelium. In four species, numerous granules of varying sizes totally electron-were observed in the epithelium and are eliminated into the lumen of glands. In glandular cells of M. cerberus, numerous granules composed of carbohydrates were detected in the cytoplasm of glandular epithelial cells. The content of secretory granules showed negative reaction to the test of basic proteins, indicating that the mixed nature. Secretory vesicles in the cytoplasm of glandular cells of M. cerberus were negative for both cytochemical tests used, indicating that its content consists of different chemicals. Sperm M. attramentarius, M. cerberus, M. injucundus and M. punctatus follow the pattern of the sperm of insects. They are long, thin, linear, measuring between 108 μm is 137μm and the head between 11 μm and 16 μm in length. They have conical acrosome, the Pentra perforatorium a cavity at the end of the core. This presents electrondense with condensed chromatin. The axoneme has the 9 +9 +2 microtubule pattern. The mitochondrial derivatives of all species in cross-sections are asymmetric. Toward the posterior region of the flagellum, there is a gradual disorganization of the axoneme, with microtubule pairs of the first disappear, followed by the central pair microtubules were left with just accessories. Data of morphology and ultrastructure of the accessory glands and sperm are an important tool that can help in understanding the reproductive process of the social wasps of the genus Mischocyttarus as well as In future phylogenetic analysis.