Caracterização da área de infarto cerebral de ratos tratados com células-tronco mesenquimais após acidente vascular encefálico isquêmico experimental

• Ano de defesa: 2013
• Autor(a) principal: Abreu Júnior, Nilton de Barros
• Orientador(a): Carlo, Ricardo Junqueira Del
• Banca de defesa: Monteiro, Betânia Souza , Duarte, Tatiana Schmitz , Viloria, Marlene Isabel Vargas
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Federal de Viçosa
• Programa de Pós-Graduação: Mestrado em Medicina Veterinária
• Departamento: Biotecnologia, diagnóstico e controle de doenças; Epidemiologia e controle de qualidade de prod. de
• País: BR
• Palavras-chave em Português: AVE - AVC; Células-tronco
• Palavras-chave em Inglês: AVE - AVC; Stem cells
• Área do conhecimento CNPq: CNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIA::CLINICA E CIRURGIA ANIMAL::CLINICA CIRURGICA ANIMAL
• Link de acesso: http://locus.ufv.br/handle/123456789/5173

Vascular ischemic brain injury usually results in severe neurological deficits and mostly irreversible. Lately, new therapies represent a new strategy for treatment of these lesions, especially with the use of stem cells in preclinical studies in animal models have shown satisfactory results. The present experiment compared the injury of cerebral ischemia in mice treated and non-treated with mesenchymal stem cells (MSCs) from bone marrow . MSCs were obtained from bone marrow of rats cultured characterized marked with nanocrystals in the sixth passage and transplanted animals with cerebral ischemia. Ischemic injury was induced by temporary occlusion of the left middle cerebral artery (MCA) for 60 minutes. The rats were randomly divided into 7 experimental groups and they were underwent surgery and divided into sham, control and treatment groups. The treated groups received a transplant of cells at a concentration of 1.0 x 106 cells / mL diluted in 1 mL administered intravenously 24 hours after ischemic induction . Performed euthanasia of animals at 24 hours, 15 and 30 days post-injury. Was measured in the area of ischemia fragment of macroscopic lesions and the cell count performed. Although MSC have not been found at the site of the lesion, the histological assessment, the group treated with MSCs showed significant reduction in the area of ischemia. The results indicate that treatment of cerebral ischemia induced using MSC therapy has beneficial effects on the recovery and protection of nerve tissue. Transplanted MSCs were localized in the lungs, the therapeutic action at a distance was performed.