Morfologia das estruturas reprodutivas masculinas em Centris, Epicharis e Trypoxylon (Hymenoptera: Apoidea)

• Ano de defesa: 2011
• Autor(a) principal: Gomes, Luiz Fernando
• Orientador(a): Lino Neto, José
• Banca de defesa: Campos, Lúcio Antonio de Oliveira , Dolder, Mary Anne Heidi , Siqueira, Maria Augusta Lima , Neves, Mariana Machado
• Tipo de documento: Tese
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Federal de Viçosa
• Programa de Pós-Graduação: Doutorado em Biologia Celular e Estrutural
• Departamento: Análises quantitativas e moleculares do Genoma; Biologia das células e dos tecidos
• País: BR
• Palavras-chave em Português: Abelha; Polimofirsmo espermático; Sinapomorfia
• Palavras-chave em Inglês: Bee; Polimorfismo sperm; Synapomorphy
• Área do conhecimento CNPq: CNPQ::CIENCIAS BIOLOGICAS::BIOLOGIA GERAL
• Link de acesso: http://locus.ufv.br/handle/123456789/250

Spermiocladistics, the use of spermatozoa morphology as a tool in phylogenetic analysis, has been applied to several orders of insects, including Hymenoptera. The number of Hymenoptera species that have had the structure of the male reproductive system and spermatozoa reported is still very small, but the few studies in this area show that the morphology of these reproductive structures presents diversified traits among taxa, which can be used in systematics within the order. As a result, our objective was to report the morphology of reproductive structures in species of Apidae and Crabronidae (Apoidea), aiming to contribute with information on the reproductive biology and systematics of this important group of insects. Optical microscopy (bright field, dark field, phase contrast and epifluorescence for DAPI) and transmission electron microscopy techniques were thus used (conventional and cytochemistry with E-PTA). Although the morphology of reproductive structures of these Hymenoptera are similar to those of previously studied species, it also shows some features not yet observed. Specific traits of reproductive structures (anatomy, histology, number of spermatozoa per cyst and ultrastructure) allow, based on interspecific differences, grouping of closely related species or higher taxa. Moreover, the association of those traits to those of external, behavioral and molecular morphology potentially sustains more consistent and concise analyses on the evolutionary relationships of Hymenoptera. Production of polymorphic spermatozoa, as observed in species of Trypoxylon of the subgenus Trypargilum, arouses curiosity regarding the reproductive biology of these insects.