Utilização de fontes oleofílicas de nitrogênio e fósforo para biorremediação de petróleo em areia de praia

• Ano de defesa: 2011
• Autor(a) principal: Oliveira, Carla Gabriela Braga de
• Orientador(a): Tótola, Marcos Rogério
• Banca de defesa: Borges, Arnaldo Chaer , Parreira, Adriano Guimarães
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Federal de Viçosa
• Programa de Pós-Graduação: Mestrado em Microbiologia Agrícola
• Departamento: Associações micorrízicas; Bactérias láticas e probióticos; Biologia molecular de fungos de interesse
• País: BR
• Palavras-chave em Português: Biorremediação; Petróleo; Bioestimulação; Nutrientes
• Palavras-chave em Inglês: Bioremediation; Petroleum; Biostimulation; Nutrients
• Área do conhecimento CNPq: CNPQ::CIENCIAS AGRARIAS::AGRONOMIA::FITOSSANIDADE::MICROBIOLOGIA AGRICOLA
• Link de acesso: http://locus.ufv.br/handle/123456789/5346

Degradation of petroleum hydrocarbons by micro-organisms in coastal environments is a very interesting strategy for remediation of these compounds. However, microbial degradation is often limited by nutrients avaiability, particularly nitrogen and phosphorus. In this work, we compared the effect of addition of water-soluble (urea and KH2PO4) or oleophilic (urea-formaldehyde resin and soy lecithin) nitrogen and phosphorus sources on oil degradation by indigenous micro-organisms and bacteria in consortia in microcosms composed by beach sand contaminated with crude oil. The microcosms were submitted to daily water exchanges representing tides activities. We also analysed the retention capability of both nutrient sources in contaminated sediment. For this, we performed UFC.mL-1 counts and quantification of nitrogen and phosphorus concentrations in the water during the experiment. The remaining oil in the sand was extracted with hexane and quantified by spectrophotometry, in which the degradation corresponds to the difference between the initial and final amounts of oil. Water-soluble nutrients have been shown to be gradually washed away during the water exchange and not remained in the sediment for bacterial supply. However, an opposite pattern was verified for oleophilic nutrients, which is a desirable feature for application in environments with intense action of waves and tides. Despite the increased retention of oleophilic nutrients, there were no significant differences in microbial counts between both treatments. In addition, oil degradation occurred mainly in those microcosms supplied with water-soluble nutrients. Although the inoculated micro-organisms are able to use the oleophilic nutrient sources, more detailed studies on the concentration of nutrients to be applied and the availability of nutrients for micro-organisms are necessary in order to maximize the stimulus generated.