Detecção de larvas de toxocara canis em leite de coelhas infectadas experimentalmente

Detalhes bibliográficos
Ano de defesa: 2011
Autor(a) principal: Exposto, Célia Fátima Silva lattes
Orientador(a): Santarém, Vamilton álvares lattes
Banca de defesa: O'dwyer, Lúcia Helena lattes, Giufrida, Rogério
Tipo de documento: Dissertação
Tipo de acesso: Acesso aberto
Idioma: por
Instituição de defesa: Universidade do Oeste Paulista
Programa de Pós-Graduação: Mestrado em Ciência Animal
Departamento: Ciências Agrárias
País: BR
Palavras-chave em Português:
Toxocaríase
Infecção transmamária
Ciclo
Palavras-chave em Inglês:
Toxocariasis
Transmammary infection
Cycle
Área do conhecimento CNPq:
CNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIA
Link de acesso: http://bdtd.unoeste.br:8080/tede/handle/tede/652
Resumo: The aim of this study was to evaluate the possibility of transmammary transmission of Toxocara canis larvae in rabbits. Nulipars white New Zealand female rabbits were distributed in two groups. Twelve animals were inoculated by oral route with 1.000 T. canis embryonated eggs, whereas five animals were uninfected (control group). One month following infection, the females were mated. Manual sample collections of 500µL milk were performed on +7, +15 and +21 days of lactation. ELISA test was run in order to detect serum antibodies (IgG) from blood samples collected from the nurses on the pre-infection (0) and post-infection (7, 14, 21 and 28) period as well as on the day following the wean. The recovery of larvae in milk was performed by the ether-formalin technique. The material was centrifuged at 2.000 rpm for 10 min (2.000 rpm; 697g) and the sediment was microscopically analyzed (10X). The presence of larvae was observed in milk samples released by 5 out of the 12 (41.7%) infected rabbits. Total number of recovered larvae was 20, ranging from 1 to 4 larvae by lactation period. Larva were observed on days +14 (9 larvae; 45%) and +21day (11 larvae; 55%). No significant difference by comparing the counting (p=0.7386). In conclusion, the presence of larvae in milk rabbit samples pointed out the possibility of galactogenic transmission of T. canis in paratenic hosts.
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spelling Santarém, Vamilton álvaresCPF:31873065515http://lattes.cnpq.br/2204972437815213O'dwyer, Lúcia HelenaCPF:88921859691http://lattes.cnpq.br/3523299060804582Giufrida, RogérioCPF:25801911863CPF:13715327880http://lattes.cnpq.br/0298211236139669Exposto, Célia Fátima Silva2016-07-18T17:53:08Z2012-02-172011-10-27EXPOSTO, Célia Fátima Silva. Detection of larvae of Toxocara canis in milk from experimentally infected rabbits. 2011. 49 f. Dissertação (Mestrado em Ciências Agrárias) - Universidade do Oeste Paulista, Presidente Prudente, 2011.http://bdtd.unoeste.br:8080/tede/handle/tede/652The aim of this study was to evaluate the possibility of transmammary transmission of Toxocara canis larvae in rabbits. Nulipars white New Zealand female rabbits were distributed in two groups. Twelve animals were inoculated by oral route with 1.000 T. canis embryonated eggs, whereas five animals were uninfected (control group). One month following infection, the females were mated. Manual sample collections of 500µL milk were performed on +7, +15 and +21 days of lactation. ELISA test was run in order to detect serum antibodies (IgG) from blood samples collected from the nurses on the pre-infection (0) and post-infection (7, 14, 21 and 28) period as well as on the day following the wean. The recovery of larvae in milk was performed by the ether-formalin technique. The material was centrifuged at 2.000 rpm for 10 min (2.000 rpm; 697g) and the sediment was microscopically analyzed (10X). The presence of larvae was observed in milk samples released by 5 out of the 12 (41.7%) infected rabbits. Total number of recovered larvae was 20, ranging from 1 to 4 larvae by lactation period. Larva were observed on days +14 (9 larvae; 45%) and +21day (11 larvae; 55%). No significant difference by comparing the counting (p=0.7386). In conclusion, the presence of larvae in milk rabbit samples pointed out the possibility of galactogenic transmission of T. canis in paratenic hosts.Com objetivo de estudar a possibilidade de transmissão de larvas pela via transmamária em coelhos, doze coelhas (Nova Zelândia) nulíparas foram infectadas com 1000 ovos larvados de T. canis, por via oral. Outras cinco coelhas receberam oralmente solução fisiológica e serviram como controle. Um mês após a inoculação, cada quatro coelhas foram acasaladas individualmente com um coelho. Após o nascimento dos filhotes nos dias 7, 14 e 21, foram coletados por ordenha manual 500µL de leite. Para detecção de anticorpos (IgG), amostras de sangue foram colhidas das matrizes nos momentos pré-infecção (0) e pós infecção (7, 14, 21 e 28) e um dia após o desmame da primeira gestação, pela técnica de ELISA. Para recuperação das larvas, foi adotada a técnica do formol-éter. Observou-se a presença de larvas em cinco das doze (41,7%) coelhas infectadas. O número total de larvas recuperadas foi de 20, variando de 1 a 4 por lactação. A presença das larvas foi observada apenas nos 140 (9 larvas; 45%) e 210 dias (11 larvas; 55%) de lactação, sem diferença significativa entre as contagens (p=0,7386). A presença de larvas de T. canis diretamente do leite de coelhas mostra a possibilidade de transmissão lactogênica em hospedeiros paratênicos.Made available in DSpace on 2016-07-18T17:53:08Z (GMT). No. of bitstreams: 1 Dissertacao.pdf: 565642 bytes, checksum: 0f056e2e06334bb526ddf08d78af93a3 (MD5) Previous issue date: 2011-10-27application/pdfhttp://bdtd.unoeste.br:8080/jspui/retrieve/1646/Dissertacao.pdf.jpgporUniversidade do Oeste PaulistaMestrado em Ciência AnimalUNOESTEBRCiências AgráriasToxocaríaseInfecção transmamáriaCicloToxocariasisTransmammary infectionCycleCNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIADetecção de larvas de toxocara canis em leite de coelhas infectadas experimentalmenteDetection of larvae of Toxocara canis in milk from experimentally infected rabbitsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisinfo:eu-repo/semantics/openAccessreponame:Biblioteca Digital de Teses e Dissertações da UNOESTEinstname:Universidade do Oeste Paulista (UNOESTE)instacron:UNOESTETHUMBNAILDissertacao.pdf.jpgDissertacao.pdf.jpgimage/jpeg2126http://bdtd.unoeste.br:8080/tede/bitstream/tede/652/2/Dissertacao.pdf.jpg455abedc965638dca1171919f66e681aMD52ORIGINALDissertacao.pdfapplication/pdf565642http://bdtd.unoeste.br:8080/tede/bitstream/tede/652/1/Dissertacao.pdf0f056e2e06334bb526ddf08d78af93a3MD51tede/6522016-07-19 01:01:34.056oai:bdtd.unoeste.br:tede/652Biblioteca Digital de Teses e Dissertaçõeshttp://bdtd.unoeste.br:8080/jspui/PUBhttp://bdtd.unoeste.br:8080/oai/requestbdtd@unoeste.bropendoar:2016-07-19T04:01:34Biblioteca Digital de Teses e Dissertações da UNOESTE - Universidade do Oeste Paulista (UNOESTE)false
dc.title.por.fl_str_mv Detecção de larvas de toxocara canis em leite de coelhas infectadas experimentalmente
dc.title.alternative.eng.fl_str_mv Detection of larvae of Toxocara canis in milk from experimentally infected rabbits
title Detecção de larvas de toxocara canis em leite de coelhas infectadas experimentalmente
spellingShingle Detecção de larvas de toxocara canis em leite de coelhas infectadas experimentalmente
Exposto, Célia Fátima Silva
Toxocaríase
Infecção transmamária
Ciclo
Toxocariasis
Transmammary infection
Cycle
CNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIA
title_short Detecção de larvas de toxocara canis em leite de coelhas infectadas experimentalmente
title_full Detecção de larvas de toxocara canis em leite de coelhas infectadas experimentalmente
title_fullStr Detecção de larvas de toxocara canis em leite de coelhas infectadas experimentalmente
title_full_unstemmed Detecção de larvas de toxocara canis em leite de coelhas infectadas experimentalmente
title_sort Detecção de larvas de toxocara canis em leite de coelhas infectadas experimentalmente
author Exposto, Célia Fátima Silva
author_facet Exposto, Célia Fátima Silva
author_role author
dc.contributor.advisor1.fl_str_mv Santarém, Vamilton álvares
dc.contributor.advisor1ID.fl_str_mv CPF:31873065515
dc.contributor.advisor1Lattes.fl_str_mv http://lattes.cnpq.br/2204972437815213
dc.contributor.referee1.fl_str_mv O'dwyer, Lúcia Helena
dc.contributor.referee1ID.fl_str_mv CPF:88921859691
dc.contributor.referee1Lattes.fl_str_mv http://lattes.cnpq.br/3523299060804582
dc.contributor.referee2.fl_str_mv Giufrida, Rogério
dc.contributor.referee2ID.fl_str_mv CPF:25801911863
dc.contributor.authorID.fl_str_mv CPF:13715327880
dc.contributor.authorLattes.fl_str_mv http://lattes.cnpq.br/0298211236139669
dc.contributor.author.fl_str_mv Exposto, Célia Fátima Silva
contributor_str_mv Santarém, Vamilton álvares
O'dwyer, Lúcia Helena
Giufrida, Rogério
dc.subject.por.fl_str_mv Toxocaríase
Infecção transmamária
Ciclo
topic Toxocaríase
Infecção transmamária
Ciclo
Toxocariasis
Transmammary infection
Cycle
CNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIA
dc.subject.eng.fl_str_mv Toxocariasis
Transmammary infection
Cycle
dc.subject.cnpq.fl_str_mv CNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIA
description The aim of this study was to evaluate the possibility of transmammary transmission of Toxocara canis larvae in rabbits. Nulipars white New Zealand female rabbits were distributed in two groups. Twelve animals were inoculated by oral route with 1.000 T. canis embryonated eggs, whereas five animals were uninfected (control group). One month following infection, the females were mated. Manual sample collections of 500µL milk were performed on +7, +15 and +21 days of lactation. ELISA test was run in order to detect serum antibodies (IgG) from blood samples collected from the nurses on the pre-infection (0) and post-infection (7, 14, 21 and 28) period as well as on the day following the wean. The recovery of larvae in milk was performed by the ether-formalin technique. The material was centrifuged at 2.000 rpm for 10 min (2.000 rpm; 697g) and the sediment was microscopically analyzed (10X). The presence of larvae was observed in milk samples released by 5 out of the 12 (41.7%) infected rabbits. Total number of recovered larvae was 20, ranging from 1 to 4 larvae by lactation period. Larva were observed on days +14 (9 larvae; 45%) and +21day (11 larvae; 55%). No significant difference by comparing the counting (p=0.7386). In conclusion, the presence of larvae in milk rabbit samples pointed out the possibility of galactogenic transmission of T. canis in paratenic hosts.
publishDate 2011
dc.date.issued.fl_str_mv 2011-10-27
dc.date.available.fl_str_mv 2012-02-17
dc.date.accessioned.fl_str_mv 2016-07-18T17:53:08Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
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status_str publishedVersion
dc.identifier.citation.fl_str_mv EXPOSTO, Célia Fátima Silva. Detection of larvae of Toxocara canis in milk from experimentally infected rabbits. 2011. 49 f. Dissertação (Mestrado em Ciências Agrárias) - Universidade do Oeste Paulista, Presidente Prudente, 2011.
dc.identifier.uri.fl_str_mv http://bdtd.unoeste.br:8080/tede/handle/tede/652
identifier_str_mv EXPOSTO, Célia Fátima Silva. Detection of larvae of Toxocara canis in milk from experimentally infected rabbits. 2011. 49 f. Dissertação (Mestrado em Ciências Agrárias) - Universidade do Oeste Paulista, Presidente Prudente, 2011.
url http://bdtd.unoeste.br:8080/tede/handle/tede/652
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dc.publisher.program.fl_str_mv Mestrado em Ciência Animal
dc.publisher.initials.fl_str_mv UNOESTE
dc.publisher.country.fl_str_mv BR
dc.publisher.department.fl_str_mv Ciências Agrárias
publisher.none.fl_str_mv Universidade do Oeste Paulista
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