Efeito do resveratrol na imunoexpressão de calmodulina e amelogenina em ratos com periodontite induzida por ligadura

• Ano de defesa: 2019
• Autor(a) principal: Barbaresco, Bruna Lorena
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Positivo; Brasil; Pós-Graduação; Programa de Pós-Graduação em Odontologia Clínica; UP
• Palavras-chave em Português: Odontologia; Calmodulina; Amelogenina; Periodontite; CNPQ::CIENCIAS DA SAUDE::ODONTOLOGIA
• Link de acesso: https://repositorio.cruzeirodosul.edu.br/handle/123456789/2116

Biomaterials, drugs and some natural substances that act on calcium metabolism have been used to improve bone repair as an adjuvant to the treatment of periodontitis. Thus, the objective of the present study was to evaluate the deposition of Ca2 + in the organic matrix in the interproximal region after experimental periodontitis and treatment with resveratrol (RESV). Ligatures were placed around the right and left upper second molars in 32 Wistar rats for induction of periodontitis, for a period of 15 days, after the 15 days of ligatures medication time and control time of 14 and 30 days started. The test group (n = 16) received the 10 mg / kg / day dose of β-cyclodextrin associated with resveratrol (β-CD / RESV) and the Control Group (n = 16) also received the same dosage by gavage of β-CD. After 14 and 30 days of treatment, the animals were euthanized and the jaws removed for immunohistochemical analysis for quantification of Calmodulin (CaM) and Amelogenin proteins. The slides were photographed and analyzed in the ImageJ program considering the percentage of immunolabelled area. Data were submitted to Student's t-test with a significance level of 0.01. The results showed that the Test Group had a higher immunopigmented percentage of CaM and amelogenin (p <0.01), a condition that coincided with an evident increase in alveolar bone matrix deposition. In this context, and within the limits of this study, it is concluded that the use of RESV may be responsible for the increase of Calcium verified by the greater percentage of immunomodulated area of CaM and amelogenin, indicating that RESV may constitute a possible alternative to minimize reabsorption bone damage caused by periodontitis.