Proteômica do carcinoma epidermóide de cabeça e pescoço: identificação e validação de biomarcadores potenciais
Ano de defesa: | 2010 |
---|---|
Autor(a) principal: | |
Orientador(a): | |
Banca de defesa: | , , , |
Tipo de documento: | Tese |
Tipo de acesso: | Acesso aberto |
Idioma: | por |
Instituição de defesa: |
Faculdade de Medicina de São José do Rio Preto
|
Programa de Pós-Graduação: |
Programa de Pós-Graduação em Ciências da Saúde
|
Departamento: |
Faculdade 1::Departamento 1
|
País: |
Brasil
|
Palavras-chave em Português: | |
Palavras-chave em Inglês: | |
Área do conhecimento CNPq: | |
Link de acesso: | http://bdtd.famerp.br/handle/tede/508 |
Resumo: | Epidermoid tumors of the oral cavity, larynx and pharynx, collectively known as head and neck squamous cell carcinomas, are strongly related to tobacco and alcohol consumption and still result in high mortality rates. The development of a primary tumor antecipates a greater risk of second cancers occurring in the contiguous epithelium, which can be a consequence of the abnormal response of the microenvironment to the carcinogen exposure in the upper aerodigestive tract. The prediction of tumor behavior for patients with these carcinomas still represents a challenge for clinicians and researchers. The presence of regional lymph node metastasis is their most important prognostic factor but is limited in predicting local recidive or survival. In addition, early metastatic disease is often missed by clinical, histological and radiological analysis. This emphasizes the need for identifying biomarkers which may effectively contribute to early diagnosis and prediction of tumor progression. Objectives: In this study, we analyzed oral squamous cell carcinomas (OSCCs) from different anatomic subsites and their matched adjacent normal mucosa aiming to compare the protein expression profiles in the context of a known prognosticator, namely, the presence of neoplastic cells in regional lymph nodes and of a refined classification in regard to prognosis, grouping into two groups small but already metastatic and large non-metastatic tumors. We also aimed to investigate the proteome of the tumor microenvironment to identify markers of aggressiveness that may be relevant for prognosis and therapy. Materials and Methods: To reach these objectives, we performed one- and two-dimensional electrophoresis (1-DE and 2-DE), fluorescent two-dimensional differential in-gel electrophoresis (2-D DIGE), mass spectrometry, Western blot and immunohistochemistry to analyze the protein expression in OSCCs. Results and Discussion: A variety of factors influence the protein sample preparation and the optimization of protein solubilization and 2-DE protocols was fundamental for the acquisition of consistent results. Many protein differences between metastatic and non-metastatic tumors and between tumor and normal cells including abnormal expression of annexin A1, calgranulin-B, cofilin-1, galectin-7, glutathione Stransferase P, cytokeratin-4 and creatine kinase were observed. These proteins are involved in cell signaling, inflammatory response, apoptosis, cell motility and adhesion, development, cell differentiation and proliferation and metabolic process, and may be related to the aggressive phenotype. Tumor microenvironment may also contribute to the neoplastic process. Our results on paracrine factors synthetyzed in vitro by stromal cells showed altered gene and protein levels in neoplastic cells after treatment with conditioned medium from tumor-associated fibroblasts, and vice-versa. Conclusions: The data may help to understand the mechanisms governing aggressiveness in OSCCs at the molecular level and the contributions of the tumor microenvironment to carcinogenic process, providing new insights into signaling and metabolic pathway abnormalities that could be useful to prognosis. |
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Silva, Eloíza Helena Tajara dahttp://lattes.cnpq.br/8102755896732025Cabral, HamiltonLabate, Carlos AlbertoMolina, Fernando DrimelMattos, Luiz Carlos de29299224862http://lattes.cnpq.br/6285509899123216Polachini, Giovana Mussi2019-02-13T17:26:36Z2010-06-22Polachini, Giovana Mussi. Proteômica do carcinoma epidermóide de cabeça e pescoço: identificação e validação de biomarcadores potenciais. 2010. 165 f. Tese (Programa de Pós-Graduação em Ciências da Saúde) - Faculdade de Medicina de São José do Rio Preto, São José do Rio Preto.1014http://bdtd.famerp.br/handle/tede/508Epidermoid tumors of the oral cavity, larynx and pharynx, collectively known as head and neck squamous cell carcinomas, are strongly related to tobacco and alcohol consumption and still result in high mortality rates. The development of a primary tumor antecipates a greater risk of second cancers occurring in the contiguous epithelium, which can be a consequence of the abnormal response of the microenvironment to the carcinogen exposure in the upper aerodigestive tract. The prediction of tumor behavior for patients with these carcinomas still represents a challenge for clinicians and researchers. The presence of regional lymph node metastasis is their most important prognostic factor but is limited in predicting local recidive or survival. In addition, early metastatic disease is often missed by clinical, histological and radiological analysis. This emphasizes the need for identifying biomarkers which may effectively contribute to early diagnosis and prediction of tumor progression. Objectives: In this study, we analyzed oral squamous cell carcinomas (OSCCs) from different anatomic subsites and their matched adjacent normal mucosa aiming to compare the protein expression profiles in the context of a known prognosticator, namely, the presence of neoplastic cells in regional lymph nodes and of a refined classification in regard to prognosis, grouping into two groups small but already metastatic and large non-metastatic tumors. We also aimed to investigate the proteome of the tumor microenvironment to identify markers of aggressiveness that may be relevant for prognosis and therapy. Materials and Methods: To reach these objectives, we performed one- and two-dimensional electrophoresis (1-DE and 2-DE), fluorescent two-dimensional differential in-gel electrophoresis (2-D DIGE), mass spectrometry, Western blot and immunohistochemistry to analyze the protein expression in OSCCs. Results and Discussion: A variety of factors influence the protein sample preparation and the optimization of protein solubilization and 2-DE protocols was fundamental for the acquisition of consistent results. Many protein differences between metastatic and non-metastatic tumors and between tumor and normal cells including abnormal expression of annexin A1, calgranulin-B, cofilin-1, galectin-7, glutathione Stransferase P, cytokeratin-4 and creatine kinase were observed. These proteins are involved in cell signaling, inflammatory response, apoptosis, cell motility and adhesion, development, cell differentiation and proliferation and metabolic process, and may be related to the aggressive phenotype. Tumor microenvironment may also contribute to the neoplastic process. Our results on paracrine factors synthetyzed in vitro by stromal cells showed altered gene and protein levels in neoplastic cells after treatment with conditioned medium from tumor-associated fibroblasts, and vice-versa. Conclusions: The data may help to understand the mechanisms governing aggressiveness in OSCCs at the molecular level and the contributions of the tumor microenvironment to carcinogenic process, providing new insights into signaling and metabolic pathway abnormalities that could be useful to prognosis.Os tumores epidermóides de cavidade oral, laringe e faringe, coletivamente conhecidos como carcinomas de células escamosas de cabeça e pescoço, estão fortemente relacionados ao consumo de tabaco e álcool e ainda resultam em taxas elevadas de mortalidade. O desenvolvimento de um tumor primário representa um risco maior de tumores secundários surgirem no epitélio contíguo, uma provável consequência da resposta anormal do microambiente à exposição do trato aerodigestivo superior a carcinógenos. A previsão do comportamento tumoral ainda representa um desafio para clínicos e pesquisadores. A presença de metástase em linfonodos regionais é o fator prognóstico mais importante, porém é limitado quanto à predição de recidiva local ou de sobrevivência. Além disso, a doença metastática precoce não é frequentemente detectada pelas análises clínica, histológica e radiológica. Este fato enfatiza a necessidade de identificação de biomarcadores que possam efetivamente contribuir para o diagnóstico precoce e para a previsão da progressão tumoral. Objetivos: No presente estudo, analisamos carcinomas de células escamosas orais de diferentes subsítios anatômicos e suas mucosas adjacentes normais correspondentes com o objetivo de comparar os perfis de expressão protéica no contexto de um prognosticador conhecido, ou seja, a presença de células neoplásicas em linfonodos regionais, e de uma classificação refinada em relação a prognóstico, reunindo em dois grupos os tumores pequenos, mas já metastáticos, e os grandes não-metastáticos. Também investigamos o proteoma do microambiente do tumor para identificar marcadores de agressividade que possam ser relevantes para prognóstico e terapia. Materiais e Métodos: Para atingir tais objetivos, realizamos eletroforeses uni e bidimensional (1-DE e 2-DE), eletroforese de fluorescência diferencial em gel bidimensional (2-D DIGE), espectrometria de massas, Western blot e imunohistoquímica. Resultados e Discussão: Uma variedade de fatores influenciam na preparação de amostras protéicas e a otimização da solubilização de proteínas e de protocolos de 2-DE foi fundamental para a aquisição de resultados consistentes. Foram observadas muitas diferenças entre tumores metastáticos e não-metastáticos e entre tumores e células normais, incluindo expressões alteradas de anexina A1, calgranulina- B, cofilina-1, galectina-7, glutationa S-transferase P, citoqueratina-4 e creatina quinase. Estas proteínas estão envolvidas em sinalização, resposta inflamatória, apoptose, desenvolvimento, processos metabólicos, e adesão, motilidade, diferenciação e proliferação celulares, e podem estar relacionadas ao fenótipo agressivo. O microambiente tumoral também pode contribuir para o processo neoplásico. Os nossos resultados de fatores parácrinos sintetizados in vitro por células estromais mostraram expressão gênica e protéica alterada nas células neoplásicas após tratamento com meio condicionado de fibroblastos associados a tumor, e vice-versa. Conclusões: Os dados aqui obtidos podem ajudar a compreender os mecanismos da agressividade do carcinoma epidermóide oral no nível molecular e a influência do microambiente tumoral no processo carcinogênico. Tais genes e proteínas podem representar novos biomarcadores de prognóstico para este tipo de câncer.Submitted by Suzana Dias (suzana.dias@famerp.br) on 2019-02-13T17:26:36Z No. of bitstreams: 1 GiovanaMussi_tese.pdf: 801614 bytes, checksum: 74058e515a346345398c13899b1bf367 (MD5)Made available in DSpace on 2019-02-13T17:26:36Z (GMT). No. of bitstreams: 1 GiovanaMussi_tese.pdf: 801614 bytes, checksum: 74058e515a346345398c13899b1bf367 (MD5) Previous issue date: 2010-06-22Fundação de Amparo à Pesquisa do Estado de São Paulo - FAPESPapplication/pdfporFaculdade de Medicina de São José do Rio PretoPrograma de Pós-Graduação em Ciências da SaúdeFAMERPBrasilFaculdade 1::Departamento 1OtorrinolaringopatiasCarcinoma de Células EscamosasProteômicaAnálise EspectralOtorhinolaryngologic DiseasesCarcinoma, Squamous CellProteomicsSpectrum AnalysisCIENCIAS DA SAUDE::MEDICINAProteômica do carcinoma epidermóide de cabeça e pescoço: identificação e validação de biomarcadores potenciaisinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/doctoralThesis-6954410853678806574500500600600306626487509624506-969369452308786627-6491868300948288337info:eu-repo/semantics/openAccessreponame:Biblioteca Digital de Teses e Dissertações da FAMERPinstname:Faculdade de Medicina de São José do Rio Preto (FAMERP)instacron:FAMERPORIGINALGiovanaMussi_tese.pdfGiovanaMussi_tese.pdfapplication/pdf80161474058e515a346345398c13899b1bf367MD52LICENSElicense.txtlicense.txttext/plain; charset=utf-82165bd3efa91386c1718a7f26a329fdcb468MD51http://bdtd.famerp.br/bitstream/tede/508/2/GiovanaMussi_tese.pdfhttp://bdtd.famerp.br/bitstream/tede/508/1/license.txttede/5082019-02-13 15:26:36.852oai:localhost: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Biblioteca Digital de Teses e Dissertaçõeshttp://bdtd.famerp.br/PUBhttps://bdtd.famerp.br/oai/requestsbdc@famerp.br||joao.junior@famerp.bropendoar:47112019-02-13T17:26:36Biblioteca Digital de Teses e Dissertações da FAMERP - Faculdade de Medicina de São José do Rio Preto (FAMERP)false |
dc.title.por.fl_str_mv |
Proteômica do carcinoma epidermóide de cabeça e pescoço: identificação e validação de biomarcadores potenciais |
title |
Proteômica do carcinoma epidermóide de cabeça e pescoço: identificação e validação de biomarcadores potenciais |
spellingShingle |
Proteômica do carcinoma epidermóide de cabeça e pescoço: identificação e validação de biomarcadores potenciais Polachini, Giovana Mussi Otorrinolaringopatias Carcinoma de Células Escamosas Proteômica Análise Espectral Otorhinolaryngologic Diseases Carcinoma, Squamous Cell Proteomics Spectrum Analysis CIENCIAS DA SAUDE::MEDICINA |
title_short |
Proteômica do carcinoma epidermóide de cabeça e pescoço: identificação e validação de biomarcadores potenciais |
title_full |
Proteômica do carcinoma epidermóide de cabeça e pescoço: identificação e validação de biomarcadores potenciais |
title_fullStr |
Proteômica do carcinoma epidermóide de cabeça e pescoço: identificação e validação de biomarcadores potenciais |
title_full_unstemmed |
Proteômica do carcinoma epidermóide de cabeça e pescoço: identificação e validação de biomarcadores potenciais |
title_sort |
Proteômica do carcinoma epidermóide de cabeça e pescoço: identificação e validação de biomarcadores potenciais |
author |
Polachini, Giovana Mussi |
author_facet |
Polachini, Giovana Mussi |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Silva, Eloíza Helena Tajara da |
dc.contributor.advisor1Lattes.fl_str_mv |
http://lattes.cnpq.br/8102755896732025 |
dc.contributor.referee1.fl_str_mv |
Cabral, Hamilton |
dc.contributor.referee2.fl_str_mv |
Labate, Carlos Alberto |
dc.contributor.referee3.fl_str_mv |
Molina, Fernando Drimel |
dc.contributor.referee4.fl_str_mv |
Mattos, Luiz Carlos de |
dc.contributor.authorID.fl_str_mv |
29299224862 |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/6285509899123216 |
dc.contributor.author.fl_str_mv |
Polachini, Giovana Mussi |
contributor_str_mv |
Silva, Eloíza Helena Tajara da Cabral, Hamilton Labate, Carlos Alberto Molina, Fernando Drimel Mattos, Luiz Carlos de |
dc.subject.por.fl_str_mv |
Otorrinolaringopatias Carcinoma de Células Escamosas Proteômica Análise Espectral |
topic |
Otorrinolaringopatias Carcinoma de Células Escamosas Proteômica Análise Espectral Otorhinolaryngologic Diseases Carcinoma, Squamous Cell Proteomics Spectrum Analysis CIENCIAS DA SAUDE::MEDICINA |
dc.subject.eng.fl_str_mv |
Otorhinolaryngologic Diseases Carcinoma, Squamous Cell Proteomics Spectrum Analysis |
dc.subject.cnpq.fl_str_mv |
CIENCIAS DA SAUDE::MEDICINA |
description |
Epidermoid tumors of the oral cavity, larynx and pharynx, collectively known as head and neck squamous cell carcinomas, are strongly related to tobacco and alcohol consumption and still result in high mortality rates. The development of a primary tumor antecipates a greater risk of second cancers occurring in the contiguous epithelium, which can be a consequence of the abnormal response of the microenvironment to the carcinogen exposure in the upper aerodigestive tract. The prediction of tumor behavior for patients with these carcinomas still represents a challenge for clinicians and researchers. The presence of regional lymph node metastasis is their most important prognostic factor but is limited in predicting local recidive or survival. In addition, early metastatic disease is often missed by clinical, histological and radiological analysis. This emphasizes the need for identifying biomarkers which may effectively contribute to early diagnosis and prediction of tumor progression. Objectives: In this study, we analyzed oral squamous cell carcinomas (OSCCs) from different anatomic subsites and their matched adjacent normal mucosa aiming to compare the protein expression profiles in the context of a known prognosticator, namely, the presence of neoplastic cells in regional lymph nodes and of a refined classification in regard to prognosis, grouping into two groups small but already metastatic and large non-metastatic tumors. We also aimed to investigate the proteome of the tumor microenvironment to identify markers of aggressiveness that may be relevant for prognosis and therapy. Materials and Methods: To reach these objectives, we performed one- and two-dimensional electrophoresis (1-DE and 2-DE), fluorescent two-dimensional differential in-gel electrophoresis (2-D DIGE), mass spectrometry, Western blot and immunohistochemistry to analyze the protein expression in OSCCs. Results and Discussion: A variety of factors influence the protein sample preparation and the optimization of protein solubilization and 2-DE protocols was fundamental for the acquisition of consistent results. Many protein differences between metastatic and non-metastatic tumors and between tumor and normal cells including abnormal expression of annexin A1, calgranulin-B, cofilin-1, galectin-7, glutathione Stransferase P, cytokeratin-4 and creatine kinase were observed. These proteins are involved in cell signaling, inflammatory response, apoptosis, cell motility and adhesion, development, cell differentiation and proliferation and metabolic process, and may be related to the aggressive phenotype. Tumor microenvironment may also contribute to the neoplastic process. Our results on paracrine factors synthetyzed in vitro by stromal cells showed altered gene and protein levels in neoplastic cells after treatment with conditioned medium from tumor-associated fibroblasts, and vice-versa. Conclusions: The data may help to understand the mechanisms governing aggressiveness in OSCCs at the molecular level and the contributions of the tumor microenvironment to carcinogenic process, providing new insights into signaling and metabolic pathway abnormalities that could be useful to prognosis. |
publishDate |
2010 |
dc.date.issued.fl_str_mv |
2010-06-22 |
dc.date.accessioned.fl_str_mv |
2019-02-13T17:26:36Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/doctoralThesis |
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publishedVersion |
dc.identifier.citation.fl_str_mv |
Polachini, Giovana Mussi. Proteômica do carcinoma epidermóide de cabeça e pescoço: identificação e validação de biomarcadores potenciais. 2010. 165 f. Tese (Programa de Pós-Graduação em Ciências da Saúde) - Faculdade de Medicina de São José do Rio Preto, São José do Rio Preto. |
dc.identifier.uri.fl_str_mv |
http://bdtd.famerp.br/handle/tede/508 |
dc.identifier.doi.por.fl_str_mv |
1014 |
identifier_str_mv |
Polachini, Giovana Mussi. Proteômica do carcinoma epidermóide de cabeça e pescoço: identificação e validação de biomarcadores potenciais. 2010. 165 f. Tese (Programa de Pós-Graduação em Ciências da Saúde) - Faculdade de Medicina de São José do Rio Preto, São José do Rio Preto. 1014 |
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Faculdade de Medicina de São José do Rio Preto |
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Faculdade de Medicina de São José do Rio Preto |
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74058e515a346345398c13899b1bf367 bd3efa91386c1718a7f26a329fdcb468 |
bitstream.checksumAlgorithm.fl_str_mv |
MD5 MD5 |
repository.name.fl_str_mv |
Biblioteca Digital de Teses e Dissertações da FAMERP - Faculdade de Medicina de São José do Rio Preto (FAMERP) |
repository.mail.fl_str_mv |
sbdc@famerp.br||joao.junior@famerp.br |
_version_ |
1809113756173598720 |