Porphyromonas gingivalis induz remodelamento cardíaco em ratos

• Ano de defesa: 2020
• Autor(a) principal: Peron, Daniele Fernanda
• Orientador(a): Serra, Andrey Jorge
• Banca de defesa: Serra, Andrey Jorge , Prates, Renato Araujo , Pavani, Christiane , Scocate, Ana Carla Raphaelli Nahás , Quaglio, Camila
• Tipo de documento: Tese
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Nove de Julho
• Programa de Pós-Graduação: Programa de Pós-Graduação em Medicina – Biofotônica
• Departamento: Saúde
• País: Brasil
• Palavras-chave em Português: porphyromonas gingivalis; coração; doenças cardiovasculares; inflamação
• Palavras-chave em Inglês: porphyromonas gingivalis; heart; cardiovascular diseases; inflammation
• Área do conhecimento CNPq: CIENCIAS DA SAUDE
• Link de acesso: http://bibliotecatede.uninove.br/handle/tede/2650

Porphyromonas gingivalis are present in various forms of periodontal diseases. Although an association between the synergistic action of P.gingivalis and cardiovascular diseases has been suggested, the effects of P. gingivalis bacteria as a cause have not yet been clarified. The aim of the present study was to evaluate whether the systemic inoculation of P. gingivalis could induce cardiac remodeling in rats. 33 rats of Wistar lineage were used, the animals were distributed in the following experimental groups: Without inoculation of P. gingivalis: Inoculation of 1x108 UFC / mL of P. gingivalis: Inoculation of 3x108 UFC / mL of P. gingivalis. Initially, they performed a basal blood collection and an initial echocardiogram in all animals, and performed a blood collection sixty minutes after the first and second inoculation, on the fifteenth day the inoculated animals received the second inoculation of P. gingivalis. On the twenty-ninth day, the animals underwent the last blood collection and on the last echocardiogram, on the thirty-th day, hemodynamics and tissue collection were performed in all experimental groups. The results showed that animals inoculated with a higher bacterial concentration 3x108 CFU / mL showed an increase in rectal temperature, a reduction in TRIV (Isovolumetric relaxation time), water content in the lung, exhibited a lower -dP / dt and a greater PD2, an increase of myocardial necrosis by CK-MB and hematosin-eosin histological data, a collagen content by trichome staining, intensification of the “ladder” pattern of DNA fragmentation, increased levels of MDA (Malondialdehyde) and oxidized proteins in the myocardium and the presence of macrophage expression in the myocardium. They concluded that animals inoculated with 3x108 UFC/ mL evolved with LV systolic and diastolic dysfunction, necrosis, apoptosis and myocardial fibrosis, increased oxidative stress and inflammatory infiltrate of macrophages in the myocardium.