Efeitos da laserterapia de baixa intensidade sobre marcadores de estresse oxidativo do m??sculo gastrocn??mio de ratos diab??ticos submetidos a exerc??cio de alta intensidade

• Ano de defesa: 2015
• Autor(a) principal: Frigero, Marcelo
• Orientador(a): Carvalho, Paulo de Tarso Camillo de
• Banca de defesa: Carvalho, Paulo de Tarso Camillo de, Leal Junior, Ernesto Cesar Pinto, Serra, Andrey Jorge, Liebano, Richard Eloin, Carvalho, Fl??vio Aimbire de
• Tipo de documento: Tese
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Nove de Julho
• Programa de Pós-Graduação: Programa de P??s-Gradua????o em Biofot??nica Aplicada ??s Ci??ncias da Sa??de
• Departamento: Sa??de
• País: Brasil
• Palavras-chave em Português: diabetes mellitus; laser baixa pot??ncia; estresse oxidativo
• Palavras-chave em Inglês: diabetes mellitus; low level laser therapy; stress oxidative
• Área do conhecimento CNPq: CIENCIAS DA SAUDE
• Link de acesso: http://bibliotecatede.uninove.br/handle/tede/1777

Purpose: The aim of the present study was to determine whether low-level laser therapy (LLLT), when used in gastrocnemius muscle of diabetic rats subjected to high-intensity exercise to fatigue, Interferes with the expression of stress markers oxidative. Materials and Methods: The total of 24 male Wistar rats (Norvergicos Albinos). Were used for this study: 24 diabetics rats. The animals were randomly divided into three groups designated as follows: Group Diabetic Fatigue - Did not receive irradiation to LLLT and performed the fatigue protocol; Group Diabetic Laser Fatigue - received irradiation to LLLT and performed the fatigue protocol and Group Non-Diabetic Control - Did not receive irradiation to LLLT and performed the fatigue protocol. Diabetes was induced in rats by streptozotocin (50 mg / kg, ip) was subsequently performed measure of blood lactate levels, LLLT laser was applied at 808 nm and 4 joules of energy to the Indicated groups and the animals were exercised groups submitted to treadmill running with speed and gradual slope until exhaustion noted by VO2m??x and lactate level. The rats were euthanized, and muscle tissue and presented the analysis of SOD markers; CAT; GPX and TBARS. Results: VO20.75m??x values in the Group Diabetic Fatigue were significantly higher from those in the control group (p <0.01) and the Group Diabetic Laser Fatigue (p <0.05). The results indicate that the activities of CAT, SOD, and GPx were higher and statistically significant (p<0.05) in the Group Diabetic Laser Fatigue than those in the diabetic groups. Outcomes for LBI group were similar than control group (p>0.05), statistically significant activities of antioxidant enzymes compared to the Group Diabetic Fatigue. The LBI group could also mitigate the concentration of TBARS (p> 0.05). Conclusion: LBI imay reduce oxidative stress, as well as increase VO20.75m??x. Furthermore, our outcomes indicate that LBI can be an alternative to maintain physical fitness in conditions where subjects aren???t able to perform exercise. Which needs to be tested in further clinical studies.