Migra????o de c??lulas precursoras miog??nicas sob a influ??ncia de sobrenadantes de macr??fagos irradiados com laser de baixa pot??ncia

Detalhes bibliográficos
Ano de defesa: 2015
Autor(a) principal: Batista, ??rika C??ssia Barroso lattes
Orientador(a): Fernandes, Kristianne Porta Santos
Banca de defesa: Fernandes, Kristianne Porta Santos, Fran??a, Cristiane Miranda, Iemma, Monica Rosas da Costa
Tipo de documento: Dissertação
Tipo de acesso: Acesso aberto
Idioma: por
Instituição de defesa: Universidade Nove de Julho
Programa de Pós-Graduação: Programa de P??s-Gradua????o em Ci??ncias da Reabilita????o
Departamento: Sa??de
País: Brasil
Palavras-chave em Português:
migra????o
c??lulas precursoras miog??nicas
macr??fagos
laser de baixa pot??ncia
Palavras-chave em Inglês:
migration
myogenic precursor cells
macrophages
low level laser
Área do conhecimento CNPq:
CIENCIAS DA SAUDE
Link de acesso: http://bibliotecatede.uninove.br/handle/tede/1802
Resumo: The activation, proliferation and migration of myogenic precursor cells are essential for muscle regeneration after injury, orchestrated by cells and local components, mainly inflammatory cells, especially macrophages. These are identified as a target for the treatment of muscle injuries. Laser therapy has shown good results in treatment of injuries and the ability to accelerate the migration of various cell types, but there are no reports on their effect on macrophage products influencing the migration of myogenic precursor cells. The aim of the study was to evaluate the effect of low level laser (LLL) on migration of myoblasts cultured with macrophage culture supernatants of different phenotypes. Therefore, C2C12 cells were cultured with supernatants from cultures of macrophages (J774) treated with LPS and IFN-?? (for the activation phenotype M1), IL-4 (M2a phenotype) and IL-10 and dexamethasone (M2c phenotype) and LLL irradiated at wavelengths of 660nm and 780nm (70mW; 17,5J / cm2; 14.3 s; 0,8J). Supernatants from macrophage cultures were harvested 24h after irradiation and transferred to myoblast cultures. Migration was assessed using the scratch assay and the results statistically analyzed. Myoblasts cultured with phenotype macrophage supernatants M2c and irradiated with LBP (660nm) showed higher migration capability that cultured with supernatants of M2C phenotype of macrophages after 12 hours of cultivation. There was no difference between the other groups. The LLL was able to modulate the migration of myoblasts C2C12 when M2c supernatants of macrophage phenotype
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spelling Fernandes, Kristianne Porta SantosFerrari, Raquel Agnelli MesquitaFernandes, Kristianne Porta SantosFran??a, Cristiane MirandaIemma, Monica Rosas da Costahttp://lattes.cnpq.br/2520841630025710Batista, ??rika C??ssia Barroso2018-06-18T22:38:09Z2015-12-16Batista, ??rika C??ssia Barroso. Migra????o de c??lulas precursoras miog??nicas sob a influ??ncia de sobrenadantes de macr??fagos irradiados com laser de baixa pot??ncia. 2015. 48 f. Disserta????o( Programa de P??s-Gradua????o em Ci??ncias da Reabilita????o) - Universidade Nove de Julho, S??o Paulo.http://bibliotecatede.uninove.br/handle/tede/1802The activation, proliferation and migration of myogenic precursor cells are essential for muscle regeneration after injury, orchestrated by cells and local components, mainly inflammatory cells, especially macrophages. These are identified as a target for the treatment of muscle injuries. Laser therapy has shown good results in treatment of injuries and the ability to accelerate the migration of various cell types, but there are no reports on their effect on macrophage products influencing the migration of myogenic precursor cells. The aim of the study was to evaluate the effect of low level laser (LLL) on migration of myoblasts cultured with macrophage culture supernatants of different phenotypes. Therefore, C2C12 cells were cultured with supernatants from cultures of macrophages (J774) treated with LPS and IFN-?? (for the activation phenotype M1), IL-4 (M2a phenotype) and IL-10 and dexamethasone (M2c phenotype) and LLL irradiated at wavelengths of 660nm and 780nm (70mW; 17,5J / cm2; 14.3 s; 0,8J). Supernatants from macrophage cultures were harvested 24h after irradiation and transferred to myoblast cultures. Migration was assessed using the scratch assay and the results statistically analyzed. Myoblasts cultured with phenotype macrophage supernatants M2c and irradiated with LBP (660nm) showed higher migration capability that cultured with supernatants of M2C phenotype of macrophages after 12 hours of cultivation. There was no difference between the other groups. The LLL was able to modulate the migration of myoblasts C2C12 when M2c supernatants of macrophage phenotypeA ativa????o, prolifera????o e migra????o das c??lulas precursoras miog??nicas s??o essenciais na regenera????o muscular ap??s les??es, orquestrados pelas c??lulas e componentes locais, principalmente pelas c??lulas inflamat??rias, em especial os macr??fagos. Estes s??o apontadas como alvo para o tratamento das les??es musculares. A laserterapia tem demonstrado bons resultados no tratamento destas les??es e na capacidade de acelerar a migra????o de v??rios tipos celulares, mas n??o existem relatos sobre seu efeito nos produtos de macr??fagos que influenciam a migra????o de c??lulas precursoras miog??nicas. O objetivo do estudo foi avaliar o efeito do laser de baixa pot??ncia (LBP) sobre a migra????o de mioblastos cultivadas com sobrenadantes de culturas de macr??fagos de diferentes fen??tipos. Para tanto, as c??lulas C2C12 foram cultivadas com sobrenadantes de culturas de macr??fagos (J774) tratadas com LPS e IFN- ?? (ativa????o para o fen??tipo M1), IL-4 (fen??tipo M2a) e IL-10 e dexametasona (fen??tipo M2c) e irradiadas com LBP nos comprimentos de onda de 660nm e 780nm (70mW; 17,5J/cm2; 14,3 s; 0,8J). Os sobrenadantes das culturas de macr??fagos foram colhidos 24h ap??s as irradia????es e transferidos para culturas de mioblastos. A migra????o foi avaliada por meio do ensaio de ferida e os resultados submetidos ?? an??lise estat??stica. Ap??s 12h de cultivo, os mioblastos cultivados com sobrenadantes de macr??fagos de fen??tipo M2c e irradiados com LBP (660nm) mostraram maior capacidade de migra????o que os cultivados com sobrenadantes de macr??fagos de fen??tipo M2c n??o irradiados. N??o houve diferen??a entre os demais grupos. O LBP foi capaz de modular a migra????o de mioblastos C2C12 quando cultivados com sobrenadantes de macr??fagos de fen??tipo M2c.Submitted by Nadir Basilio (nadirsb@uninove.br) on 2018-06-18T22:38:09Z No. of bitstreams: 1 Erika Cassia Barroso Batista.pdf: 1153764 bytes, checksum: d2f3d36a962ecb178963820d00e754fa (MD5)Made available in DSpace on 2018-06-18T22:38:09Z (GMT). No. of bitstreams: 1 Erika Cassia Barroso Batista.pdf: 1153764 bytes, checksum: d2f3d36a962ecb178963820d00e754fa (MD5) Previous issue date: 2015-12-16application/pdfporUniversidade Nove de JulhoPrograma de P??s-Gradua????o em Ci??ncias da Reabilita????oUNINOVEBrasilSa??demigra????oc??lulas precursoras miog??nicasmacr??fagoslaser de baixa pot??nciamigrationmyogenic precursor cellsmacrophageslow level laserCIENCIAS DA SAUDEMigra????o de c??lulas precursoras miog??nicas sob a influ??ncia de sobrenadantes de macr??fagos irradiados com laser de baixa pot??nciaMigration of myogenic precursor cells under the influence of macrophage supernatants irradiated with low level laserinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesis8765449414823306929600info:eu-repo/semantics/openAccessreponame:Biblioteca Digital de Teses e Dissertações da Uninoveinstname:Universidade Nove de Julho (UNINOVE)instacron:UNINOVEORIGINALErika Cassia Barroso Batista.pdfErika Cassia Barroso Batista.pdfapplication/pdf1153764http://localhost:8080/tede/bitstream/tede/1802/2/Erika+Cassia+Barroso+Batista.pdfd2f3d36a962ecb178963820d00e754faMD52LICENSElicense.txtlicense.txttext/plain; charset=utf-82165http://localhost:8080/tede/bitstream/tede/1802/1/license.txtbd3efa91386c1718a7f26a329fdcb468MD51tede/18022018-06-18 19:38:09.337oai:localhost: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Biblioteca Digital de Teses e Dissertaçõeshttp://bibliotecatede.uninove.br/PRIhttp://bibliotecatede.uninove.br/oai/requestbibliotecatede@uninove.br||bibliotecatede@uninove.bropendoar:2018-06-18T22:38:09Biblioteca Digital de Teses e Dissertações da Uninove - Universidade Nove de Julho (UNINOVE)false
dc.title.por.fl_str_mv Migra????o de c??lulas precursoras miog??nicas sob a influ??ncia de sobrenadantes de macr??fagos irradiados com laser de baixa pot??ncia
dc.title.alternative.eng.fl_str_mv Migration of myogenic precursor cells under the influence of macrophage supernatants irradiated with low level laser
title Migra????o de c??lulas precursoras miog??nicas sob a influ??ncia de sobrenadantes de macr??fagos irradiados com laser de baixa pot??ncia
spellingShingle Migra????o de c??lulas precursoras miog??nicas sob a influ??ncia de sobrenadantes de macr??fagos irradiados com laser de baixa pot??ncia
Batista, ??rika C??ssia Barroso
migra????o
c??lulas precursoras miog??nicas
macr??fagos
laser de baixa pot??ncia
migration
myogenic precursor cells
macrophages
low level laser
CIENCIAS DA SAUDE
title_short Migra????o de c??lulas precursoras miog??nicas sob a influ??ncia de sobrenadantes de macr??fagos irradiados com laser de baixa pot??ncia
title_full Migra????o de c??lulas precursoras miog??nicas sob a influ??ncia de sobrenadantes de macr??fagos irradiados com laser de baixa pot??ncia
title_fullStr Migra????o de c??lulas precursoras miog??nicas sob a influ??ncia de sobrenadantes de macr??fagos irradiados com laser de baixa pot??ncia
title_full_unstemmed Migra????o de c??lulas precursoras miog??nicas sob a influ??ncia de sobrenadantes de macr??fagos irradiados com laser de baixa pot??ncia
title_sort Migra????o de c??lulas precursoras miog??nicas sob a influ??ncia de sobrenadantes de macr??fagos irradiados com laser de baixa pot??ncia
author Batista, ??rika C??ssia Barroso
author_facet Batista, ??rika C??ssia Barroso
author_role author
dc.contributor.advisor1.fl_str_mv Fernandes, Kristianne Porta Santos
dc.contributor.advisor-co1.fl_str_mv Ferrari, Raquel Agnelli Mesquita
dc.contributor.referee1.fl_str_mv Fernandes, Kristianne Porta Santos
dc.contributor.referee2.fl_str_mv Fran??a, Cristiane Miranda
dc.contributor.referee3.fl_str_mv Iemma, Monica Rosas da Costa
dc.contributor.authorLattes.fl_str_mv http://lattes.cnpq.br/2520841630025710
dc.contributor.author.fl_str_mv Batista, ??rika C??ssia Barroso
contributor_str_mv Fernandes, Kristianne Porta Santos
Ferrari, Raquel Agnelli Mesquita
Fernandes, Kristianne Porta Santos
Fran??a, Cristiane Miranda
Iemma, Monica Rosas da Costa
dc.subject.por.fl_str_mv migra????o
c??lulas precursoras miog??nicas
macr??fagos
laser de baixa pot??ncia
topic migra????o
c??lulas precursoras miog??nicas
macr??fagos
laser de baixa pot??ncia
migration
myogenic precursor cells
macrophages
low level laser
CIENCIAS DA SAUDE
dc.subject.eng.fl_str_mv migration
myogenic precursor cells
macrophages
low level laser
dc.subject.cnpq.fl_str_mv CIENCIAS DA SAUDE
description The activation, proliferation and migration of myogenic precursor cells are essential for muscle regeneration after injury, orchestrated by cells and local components, mainly inflammatory cells, especially macrophages. These are identified as a target for the treatment of muscle injuries. Laser therapy has shown good results in treatment of injuries and the ability to accelerate the migration of various cell types, but there are no reports on their effect on macrophage products influencing the migration of myogenic precursor cells. The aim of the study was to evaluate the effect of low level laser (LLL) on migration of myoblasts cultured with macrophage culture supernatants of different phenotypes. Therefore, C2C12 cells were cultured with supernatants from cultures of macrophages (J774) treated with LPS and IFN-?? (for the activation phenotype M1), IL-4 (M2a phenotype) and IL-10 and dexamethasone (M2c phenotype) and LLL irradiated at wavelengths of 660nm and 780nm (70mW; 17,5J / cm2; 14.3 s; 0,8J). Supernatants from macrophage cultures were harvested 24h after irradiation and transferred to myoblast cultures. Migration was assessed using the scratch assay and the results statistically analyzed. Myoblasts cultured with phenotype macrophage supernatants M2c and irradiated with LBP (660nm) showed higher migration capability that cultured with supernatants of M2C phenotype of macrophages after 12 hours of cultivation. There was no difference between the other groups. The LLL was able to modulate the migration of myoblasts C2C12 when M2c supernatants of macrophage phenotype
publishDate 2015
dc.date.issued.fl_str_mv 2015-12-16
dc.date.accessioned.fl_str_mv 2018-06-18T22:38:09Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
format masterThesis
status_str publishedVersion
dc.identifier.citation.fl_str_mv Batista, ??rika C??ssia Barroso. Migra????o de c??lulas precursoras miog??nicas sob a influ??ncia de sobrenadantes de macr??fagos irradiados com laser de baixa pot??ncia. 2015. 48 f. Disserta????o( Programa de P??s-Gradua????o em Ci??ncias da Reabilita????o) - Universidade Nove de Julho, S??o Paulo.
dc.identifier.uri.fl_str_mv http://bibliotecatede.uninove.br/handle/tede/1802
identifier_str_mv Batista, ??rika C??ssia Barroso. Migra????o de c??lulas precursoras miog??nicas sob a influ??ncia de sobrenadantes de macr??fagos irradiados com laser de baixa pot??ncia. 2015. 48 f. Disserta????o( Programa de P??s-Gradua????o em Ci??ncias da Reabilita????o) - Universidade Nove de Julho, S??o Paulo.
url http://bibliotecatede.uninove.br/handle/tede/1802
dc.language.iso.fl_str_mv por
language por
dc.relation.cnpq.fl_str_mv 8765449414823306929
dc.relation.confidence.fl_str_mv 600
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade Nove de Julho
dc.publisher.program.fl_str_mv Programa de P??s-Gradua????o em Ci??ncias da Reabilita????o
dc.publisher.initials.fl_str_mv UNINOVE
dc.publisher.country.fl_str_mv Brasil
dc.publisher.department.fl_str_mv Sa??de
publisher.none.fl_str_mv Universidade Nove de Julho
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