Análise in vitro da desinfecção promovida por diferentes protocolos de limpeza do canal radicular com o uso do ultrassom em dentes humanos infectados por Enterococcus faecalis

• Ano de defesa: 2011
• Autor(a) principal: Silva, Kathrein Tapia da
• Orientador(a): Figueiredo, José Antonio Poli de
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Pontifícia Universidade Católica do Rio Grande do Sul; Porto Alegre
• Palavras-chave em Português: ODONTOLOGIA; ENDODONTIA; MICROSCOPIA ELETRÔNICA; CLOREXIDINA
• Link de acesso: http://hdl.handle.net/10923/359

Introduction: This study aims at analyzing, in vitro, the ultrasonic action on microbial agents (sodium hypochlorite 2. 5% and chlorhexidine 2%) in human teeth infected by enterococcus faecalis.Methodology: Sixty human premolars were used, which were contaminated with an E. faecalis pure culture with 3. 9 to 6. 3 x 107(CFU/ml) during a period of 60 days to form bacterial biofilm. Teeth were randomly divided in 8 groups, whereby those in which ultrasonic was used had a n= 10, the control groups (without ultrasound) had a n=5. Group 1- ultrasonic irrigation with sodium hypochlorite 2. 5%; group 2- ultrasonic irrigation with chlorhexidine liquid 2%; group 3- ultrasonic irrigation with chlorhexidine gel 2%; group 4- ultrasonic irrigation with distilled water; group 5- conventional irrigation with sodium hypochlorite 2. 5%; group 6- conventional irrigation with chlorhexidine liquid 2%; group 7- conventional irrigation with chlorhexidine gel 2% and group 8- conventional irrigation with distilled water. An analysis with a scanning electron microscopy (SEM) was performed.Results: In the SEM analysis with a 2000x magnification in backscattering, it was analyzed the removal of the smear layer and there was a significant difference among the groups in all the root portions. Groups 1 and 2 showed better cleaning capacity and were similar in their results. The group which showed the worst performance was group 7. In the 10000X magnfication in backscattering it was analyzed the presence of bacteria in the root canal and there was again statistic difference among the groups in all the root portions. The groups which showed the smallest bacterial biofilm were groups 1 and 2, and they were similar in their results. The group which showed the worst performance in the apical third was group 8, and in the medium and cervical third group 7.Conclusion: Regardless of the solution, ultrasonic irrigation proved to be superior to the conventional irrigation; we believe that ultrasonic can be used as an excellent auxiliary to the root canal cleaningness and disinfection.