Identificação da atividade antimicrobiana no veneno da serpente Bothrops moojeni em bactérias gram negativas

• Ano de defesa: 2010
• Autor(a) principal: Queiroz, Silvio José de
• Orientador(a): Carmo Filho, José Rodrigues do
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Pontifícia Universidade Católica de Goiás
• Programa de Pós-Graduação: Programa de Pós-Graduação STRICTO SENSU em Ciências Ambientais e Saúde
• Departamento: Escola de Ciências Médicas, Famacêuticas e Biomédicas::Curso de Biomedicina
• País: Brasil
• Palavras-chave em Português: Resistência bacteriana; Bothrops moojeni; serpentes; cromatografia; L-aminoácido oxidase;
• Palavras-chave em Inglês: Bacterial resistance; Bothrops moojeni; snakes; chromatography; Lamino acid oxidase;
• Área do conhecimento CNPq: CIENCIAS DA SAUDE
• Link de acesso: https://tede2.pucgoias.edu.br/handle/tede/3410

Infections caused by multiresistant microorganisms are associated with bacteria acquired in the hospital environment. The selective pressure of antimicrobials is an important factor in the selection and spread of resistance genes in the environment. The use of different molecules produced by living organisms has demonstrated activity against microorganisms. OBJECTIVES: To assess the activity of the crude venom of Bothrops moojeni on gram-negative bacteria producing and non producing metallo- -lactamase and -lactamases with wide spectrum, to identify component (s) of the poison with activity against gram-negative bacteria producing and non producing metallo- -lactamase and -lactamases with wide spectrum and to identify the antibacterial activity of crude venom of the snake B. moojeni in gram negative non-fermentative and fermentative glucose producing different enzymes to degrade -lactams. METHODOLOGY: The poison used was extracted from snake B. moojeni kept on the collection of the Center for Biological Studies and Research (CEPB) Catholic University of Goias Micro-organisms used in this study were obtained from the American Type Culture Collection and Bank of Microorganisms, Laboratory of Clinical Microbiology and MSc in Environmental and Health Sciences at the Catholic University of Goias, including Acinetobacter baumannii (blaIMP2), Pseudomonas aeruginosa (ATCC 27853), Pseudomonas aeruginosa blaIMP1, blaVIM1, blaVIM2, blaSPM1 and producing carbapenemase No. 278 bank) Escherichia coli (ATCC 25922 and ATCC 35 218), Klebisiella pneumoniae (ATCC 700603). The test was conducted with the crude venom applied on disk diffusion in different dilutions. Protein concetration was determined for each venon sample, buy using the Bradford. RESULTS: The microbiological test showed that the venom demonstrated antimicrobial activity, both for producing bacteria and non-producing -lactamase and -lactamases bactérias with wide spectrum and that this activity is probably due to the action of L-amino acid oxidase. CONCLUSIONS: Different concentrations of crude venom of the snake B. moojeni inhibited growth of gram-negative bactéria producing ond non producing metallobeta- lactamase and extende spectrum beta-lactamese ond the size of the allo of growth inhibited diminished when decreased concentration of the crud venon and consequentley the decrese of concentration of protein, demonstrating that the effect of crude venom was dose dependent.