Avaliação das atividades angiogênica/antiangiogênica, genotóxica/antigenotóxica do óleo de cártamo (Carthamus tinctorius)

• Ano de defesa: 2017
• Autor(a) principal: Sampaio, Lucas Rodrigues
• Orientador(a): Reis, Paulo Roberto de Melo
• Banca de defesa: Moura, Katia Karina Verolli de Oliveira , Gonçalves, Alberto Magno
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Pontifícia Universidade Católica de Goiás
• Programa de Pós-Graduação: Programa de Pós-Graduação STRICTO SENSU em Genética
• Departamento: Escola de Ciências Agrárias e Biológicas::Curso de Biologia Bacharelado
• País: Brasil
• Palavras-chave em Português: Carthamus tinctorius, Óleo de cártamo, Angiogênese, Genotoxicidade, Mutagênese, Teste de micronúcleo.
• Palavras-chave em Inglês: Carthamus tinctorius, Safflower oil, Angiogenesis, Genotoxicity, Mutagenesis, Micronucleus test.
• Área do conhecimento CNPq: CIENCIAS BIOLOGICAS::GENETICA
• Link de acesso: https://tede2.pucgoias.edu.br/handle/tede/3712

The study of traditional uses of plants and their products has gradually increased during the last years, which has resulted in a significant number of publications in this area. Phytotherapics correspond to a significant principle of biologically ative natural products, many of which constituted models for the synthesis of a large number of drugs. Therefore, inserts it the Carthamus tinctorius, a plant that is part of the family Asteraceae, presenting themselves as a crop adapted to adverse conditions, being cultivated worldwide due to its medicinal properties. The Carthamus tinctorius contains a high quality oil rich in polyunsaturated fatty acids (PUFAs). The herb has potential for improvement through molecular breeding programs due to the availability of significant genetic and phenotypic diversity. In order to evaluate the angiogenic and genotoxic activities of safflower oil, angiogenesis tests were carried out on the chorioallantoid membrane (MCA) of embryonated chicken egg and micronucleus test on hematopoietic bone marrow of mice, where the results of the test in MCA Indicated that the oil caused a significant increase of the vascular network (p <0.05) in relation to the neutral and inhibitory controls. In the micronucleus test, the doses of 500, 1,000 and 1,500 mg.kg-1 were used. No genotoxic activity was observed at doses of 500 and 1,000, since the number of micronuclei in relation positive control doxorubicin (DXR) towas lower (p <0.05). However at the dose of 1,500, the genotoxic effect was observed, since the micronucleus values detected were similar to those of the positive control. In view of the techniques and conditions employed, the conclusions of this study reinforce that safflower oil presents therapeutic potential, with angiogenic activity and absence of genotoxicity in doses of 500 and 1,000 mg.kg-1. However, it should be consumed moderately because of the mutagenic activity found in high doses.